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Registros recuperados: 7
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Modes of phagocytosis of Gram-positive and Gram-negative bacteria by Spodoptera littoralis granular haemocytes Inra
Costa, S.C.P.; Ribeiro, C.; Girard, P.A.; Zumbihl, R.; Brehélin, M..
Haemocytes are the main immunocompetent cells in insect cellular immune reactions. Here, we show that in Spodoptera littoralis, granular haemocytes are the primary phagocyte haemocytes, both in vivo and in vitro. The "trigger" and "zipper" modes of engulfment known in mammal macrophages are active, in vivo, in S. littoralis granular haemocytes, together with macropinocytosis. Lipopolysaccharide as well as lipoteichoic acid inhibit the binding of both Gram-positive (Corynebacterium xerosis) and Gramnegative (Escherichia coli) bacteria on granular haemocytes. In addition, different ligands can inhibit the binding of E coli. Most of these inhibitors are known as ligands of scavenger receptors in mammal macrophages and we hypothesise that one of the receptors...
Tipo: Journal Article Palavras-chave: SPODOPTERA LITTORALIS; NOCTUELLE MACROPINOCYTOSIS; TRIGGER; ZIPPER; RECEPTOR; INSECT.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201069bfa10d&uri=/notices/prodinra1/2011/05/
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Recent insight into the pathogenicity mechanisms of the emergent pathogen Photorhabdus asymbiotica Inra
Costa, S.C.P.; Chavez, C.V.; Jubelin, G.; Givaudan, A.; Escoubas, J.M.; Brehélin, M.; Zumbihl, R..
Photorhabdus asymbiotica is unique among the entomopathogenic bacteria of this genus in also being able to infect humans, leading to its isolation from some clinical samples. Recent comparative genomics data and the results of studies of interactions between bacteria and cells provide insight into the adaptation of this bacterium to its new niche, the human body
Tipo: Journal Article Palavras-chave: PHOTORABDUS ASYMBIOTICA; EMERGENT PATHOGEN; INSECT; MAN; COMPARATIVE GENOMICS; PHAGOCYTOSIS; APOPTOSIS; VIRULENCE FACTORS.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010764fee76&uri=/notices/prodinra1/2010/10/
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Site-specific antiphagocytic function of the Photorhabdus luminescens type III secretion system during insect colonization Inra
Brugirard-Ricaud, K.; Duchaud, E.; Givaudan, A.; Girard, P.A.; Kunst, F.; Boemare, N.; Brehélin, M.; Zumbihl, R..
Photorhabdus is an entomopathogenic bacterium belonging to the Enterobacteriaceae. The genome of the TT01 strain of Photorhabdus luminescens was recently sequenced and a large number of toxin-encoding genes were found. Genomic analysis predicted the presence on the chromosome of genes encoding a type three secretion system (TTSS), the main role of which is the delivery of effector proteins directly into eukaryotic host cells. We report here the functional characterization of the TTSS. The locus identified encodes the secretion/translocation apparatus, gene expression regulators and an effector protein - LopT - homologous to the Yersinia cysteine protease cytotoxin YopT. Heterologous expression in Yersinia demonstrated that LopT was translocated into mammal...
Tipo: Journal Article Palavras-chave: ENTOMOPATHOGENIC BACTERIUM; GENE EXPRESSION; ENTOMOPATHOGENIC NEMATODE; INSECT CELL MEMBRANE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008e8fb85eb&uri=/notices/prodinra1/2009/03/
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Spodoptera frugiperda X-tox protein, an immune related defensin rosary, has lost the function ancestral defensins Inra
Destoumieux-Garzon, D.; Brehélin, M.; Bulet, B.; Boublik, Y.; Girard, P.A.; Baghdiguian, S.; Zumbihl, R.; Escoubas, J.M..
Background: X-tox proteins are a family of immune-related proteins only found in Lepidoptera and characterized by imperfectly conserved tandem repeats of several defensin-like motifs. Previous phylogenetic analysis of X-tox genes supported the hypothesis that X-tox have evolved from defensins in a lineage-specific gene evolution restricted to Lepidoptera. In this paper, we performed a protein study in which we asked whether X-tox proteins have conserved the antimicrobial functions of their ancestral defensins and have evolved as defensin reservoirs.Methodology/Principal Findings: We followed the outcome of Spod-11-tox, an X-tox protein characterized in Spodoptera frugiperda, in bacteria-challenged larvae using both immunochemistry and antimicrobial assays....
Tipo: Journal Article Palavras-chave: LEPIDOPTERE X-TOX PROTEIN; LEPIDOPTERA; IMMUNE-RELATED PROTEIN; ANCESTRAL DEFENSINS; SPODOPTERA FRUGIPERDA.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201095cf8004&uri=/notices/prodinra1/2010/10/
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Stages of infection during the tripartite interaction between Xenorhabdus nematophila, its nematode vector, and insect hosts Inra
Sicard, M.; Brugirard-Ricaud, K.; Pages, S.; Lanois, A.; Boemare, N.; Brehélin, M.; Givaudan, A..
Bacteria of the genus Xenorhabdus are mutually associated with entomopathogenic nematodes of the genus Steinernema and are pathogenic to a broad spectrum of insects. The nematodes act as vectors, transmitting the bacteria to insect larvae, which die within a few days of infection. We characterized the early stages of bacterial infection in the insects by constructing a constitutive green fluorescent protein (GFP)-labeled Xenorhabdus nematophila strain. We injected the GFP-labeled bacteria into insects and monitored infection. We found that the bacteria had an extracellular life cycle in the hemolymph and rapidly colonized the anterior midgut region in Spodoptera littoralis larvae. Electron microscopy showed that the bacteria occupied the extracellular...
Tipo: Journal Article Palavras-chave: XENORHABDUS; ENTOMOPATHOGENIC NEMATODE; STEINERNEMA; GFP-LABELED; INTESTINAL VESICLE; SPODOPTERA.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008345034c5&uri=/notices/prodinra1/2009/03/
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The emerging human pathogen Photorhabdus asymbiotica is a facultative intracellular bacterium and induces apoptosis of macrophage-like cells Inra
Costa, S.C.P.; Girard, P.A.; Brehélin, M.; Zumbihl, R..
Photorhabdus species are gram-negative entomopathogenic bacteria of the family Enterobacteriaceae. Among the different members of the genus, one species, Photorhabdus asymbiotica, is a pathogen of both insects and humans. The pathogenicity mechanisms of this bacterium are unknown. Here we show that P. asymbiotica is a facultative intracellular pathogen that is able to replicate inside human macrophage-like cells. Furthermore, P. asymbiotica was shown for the first time in an intracellular location after insect infection. We also demonstrated that among Australian and American clinical isolates, only the Australian strains were able to invade nonphagocytic human cells. In cell culture infection experiments, Australian clinical isolates as well as cell-free...
Tipo: Journal Article Palavras-chave: PHOTORHABDUS; MACROPHAGE-LIKE CELL; PATHOGENICITY MECHANISM; CELL CULTURE; PATHOGEN INSECT; PATHOGEN HUMAN.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010d0f9e94c&uri=/notices/prodinra1/2010/10/
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The xaxAB genes encoding a new apoptotic toxin from the insect pathogen Xenorhabdus nematophila are present in plant and human pathogens Inra
Vigneux, F.; Zumbihl, R.; Jubelin, G.; Ribeiro, C.; Poncet, J.; Baghdiguian, S.; Givaudan, A.; Brehélin, M..
Xenorhabdus nematophila, a member of the Enterobacteriaceae, kills many species of insects by strongly depressing the immune system and colonizing the entire body. A peptide cytotoxin has been purified from X. nematophila broth growth, and the cytolytic effect on insect immunocytes and hemolytic effect on mammalian red blood cells of this toxin have been described (Ribeiro, C., Vignes, M., and Brehelin, M. (2003) J. Mal. Chem. 278, 3030-3039). We show here that this toxin, Xenorhabdus alpha-xenorhabdolysin (Xax), triggers apoptosis in both insect and mammalian cells. We also report the cloning and sequencing of two genes, xaxAB, encoding this toxin in X. nematophila. The expression of both genes in recombinant Escherichia coli led to the production of...
Tipo: Journal Article Palavras-chave: IMMUNE SYSTEM; ENTOMOPATHOGENIC BACTERIA; CYTOTOXIN.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008d7f6e01&uri=/notices/prodinra1/2010/11/
Registros recuperados: 7
Primeira ... 1 ... Última
 

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