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Registros recuperados: 19
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A white spot disease-like syndrome in the Pacific blue shrimp (Litopenaeus stylirostris) as a form of bacterial shell disease ArchiMer
Goarant, Cyrille; Brizard, Raphael; Marteau, Anne-laure.
In May 1997, some white lesions evoking the white spot syndrome disease were observed in Litopenaeus stylirostris broodstock in New Caledonia. The occurrence of these lesions was neither associated with mortality, nor with histological evidence of white spot syndrome baculovirus (WSBV), The evidence suggests that these lesions result from a form of bacterial disease and are associated with an increased bacterial flora on the outer surface of the cuticle, as well as an increased incidence throughout the molt cycle. A microscopic observation of these lesions allows them to be differentiated from WSBV-associated lesions.
Tipo: Text Palavras-chave: New Caledonia; White spot syndrome; Aquaculture; Pathogenic bacteria; Shrimp culture; Crustaceans; Diseases; Litopenaeus stylirostris.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-373.pdf
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Acquisition of susceptibility to Vibrio penaeicida in Penaeus stylirostris postlarvae and juveniles ArchiMer
Goarant, Cyrille; Regnier, France; Brizard, Raphael; Marteau, Anne-laure.
Vibriosis is a major disease problem in shrimp aquaculture, affecting all developmental stages, from larvae in hatchery tanks to juveniles and broodstock in growout ponds. However, bacterial strains responsible for vibriosis in the successive stages are usually considered to be different, and virulence specificity has been reported both at the species and at the stage levels. The so-called « Syndrome 93 » is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Penaeus stylirostris in growout ponds and broodstock tanks in New Caledonia. This pathology does not cause any mortality in hatchery or nursery phases. An experimental infection design using balneation of postlarvae and early juveniles in V. penaeicida suspensions was used to...
Tipo: Text Palavras-chave: Penaeus stylirostris; Vibrio sp.; Diseases and their control crustaceans.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/1998/publication-383.pdf
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Caractérisation des sédiments des fonds de bassin des différentes fermes aquacoles de Nouvelle-Calédonie ArchiMer
Lemonnier, Hugues; Brizard, Raphael.
Le but de celle fiche biotechnique est de présenter l'ensemble des données concernant l'étude de la caractérisation des sédiments aquacoles. Ce travail! a été mené pendant l'année 1996 et a abouti à un inventaire des sédiments sur l 'ensemble des sites de production du Territoire. Il s'agit d'un premier rapport descriptif permettant une première présentation des principaux résultats aux producteurs du Territoire, un second rapport comprenant les interprétations et les conclusions est actuellement en cours de rédaction.
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Ano: 1997 URL: http://archimer.ifremer.fr/doc/00274/38545/37066.pdf
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Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm ArchiMer
Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P..
Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation...
Tipo: Text Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf
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Compte rendu final du projet Triplofimer : Fiabilité et qualité de la production de naissains triploïdes d'huître creuse (Crassostrea gigas) ArchiMer
Suquet, Marc; Agogue, Julie; Brizard, Raphael; Haffray, Pierrick; Labbe, Catherine; Le Coz, Jean-rene; Pepin, Jean-francois; Rault, Paul; Saulnier, Denis.
Ce rapport fait état d'une étude réalisée au laboratoire de Génétique et Pathologie de l'IFREMER dans le cadre de l'analyse d'épisodes de mortalité anormale survenue en écloserie privée de crevettes pénéides. Plusieurs hypothèses ont été avancées. La présence de Vibrio harveyi a amené à constituer une collection de 40 souches à partir desquelles une identification bactériologique a été effectuée. Elle montre en effet une dominance de souches appartenant à un groupe homogène de Vibrio harveyi. La technique de ribotypage (digestion d'ADN génomique, transfert de Southem-blot et hybridation avec une sonde spécifique) est alors mise en place. Plusieurs difficultés techniques ont ralenti l'analyse et n'ont donc pas permis d'avoir des résultats assez avancés pour...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Tétraploïdes; Sperme; Conservation; Diagnostic zoo-sanitaire; Flore bactérienne; Herpès virus..
Ano: 2007 URL: http://archimer.ifremer.fr/doc/00304/41554/40762.pdf
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Differential Activities of DNA Polymerases in Processing Ribonucleotides during DNA Synthesis in Archaea ArchiMer
Lemor, Melanie; Kong, Ziqing; Henry, Etienne; Brizard, Raphael; Laurent, Sebastien; Bosse, Audrey; Henneke, Ghislaine.
Consistent with the fact that ribonucleotides (rNTPs) are in excess over deoxyribonucleotides (dNTPs) in vivo, recent findings indicate that replicative DNA polymerases (DNA Pols) are able to insert ribonucleotides (rNMPs) during DNA synthesis, raising crucial questions about the fidelity of DNA replication in both Bacteria and Eukarya. Here, we report that the level of rNTPs is 20-fold higher than that of dNTPs in Pyrococcus abyssi cells. Using dNTP and rNTP concentrations present in vivo, we recorded rNMP incorporation in a template-specific manner during in vitro synthesis, with the family-D DNA Pol (PolD) having the highest propensity compared with the family-B DNA Pol and the p41/p46 complex. We also showed that ribonucleotides accumulate at a...
Tipo: Text Palavras-chave: Archaea; DNA replication and repair; DNA polymerase; Nucleotide pool; Translesion synthesis.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00464/57603/59796.pdf
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Effets des renouvellements en eau sur l'écosystème des bassins d'élevages semi-intensifs de crevettes en Nouvelle-Calédonie durant la saison froide ArchiMer
Lemonnier, Hugues; Brizard, Raphael.
Une expérience a été réalisée en conditions hivernales dans six bassins de terre pour déterminer l'effet des taux de renouvellement sur la production de crevettes Penaeus stylirostris, sur les caractéristiques de l'eau et du sédiment des enceintes d'élevage. Les budgets d'azote ont été établis en prenant en compte les différentes formes azotées dans l'eau, dans le sédiment, dans l'aliment et dans les crevettes. Un épisode de mortalité a été obserVé en cours d'élevage et peut être attribué à une epizootie dénommée "syndrome 93». Ce dernier était concomitant à une phase de croissance phytoplantonique et a été d'autant plus important que la biomasse phytoplanctonique dans le bassin d'élevage était élevée. Bien qu'une dilution importante de la colonne d'eau se...
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Ano: 1998 URL: http://archimer.ifremer.fr/doc/00079/19066/16661.pdf
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Evolution of shrimp production and pond bottom in a semi-intensive system ArchiMer
Lemonnier, Hugues; Brizard, Raphael; Mugnier, Chantal; Herlin, Jose.
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Ano: 2002 URL: http://archimer.ifremer.fr/doc/00354/46558/46369.pdf
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Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis ArchiMer
Tirape, Ana; Bacque, Coralie; Brizard, Raphael; Vandenbulcke, Franck; Boulo, Viviane.
The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigar. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval...
Tipo: Text Palavras-chave: Rel/NF kappa B pathway; Cell proliferation; Hematopoiesis; Mollusk immunity; Hemocytes; Bivalve mollusk.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2612.pdf
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Impact de la dissémination accidentelle d'huîtres tétraploïdes : potentiel de dispersion et succès reproducteur ArchiMer
Lapegue, Sylvie; Heurtebise, Serge; Cornette, Florence; Brizard, Raphael; Sharbel, Tim; Boudry, Pierre.
L'ostréiculture française réalise une production très largement extensive, basée sur le recrutement de juvéniles dans le milieu naturel et la production sur des sites côtiers. Ainsi, l’huître est l’une des rares espèces cultivées à être encore considéré par le consommateur comme un produit alimentaire totalement « naturel ». L'utilisation de la polyploïdisation a permis d'ouvrir une nouvelle voie dans cette filière, en permettant d'obtenir des animaux à croissance plus rapide, plus homogènes et stériles. Cette dernière caractéristique est un gage de qualité gustative et permet aussi de positionner ce produit en priorité sur les mois en "r" traditionnellement bannis à cause de la présence de laitance. Le but principal de la triploïdisation a été atteint:...
Tipo: Text Palavras-chave: Génétique; Triploïdisation; Tétraploïde; Impact; Environnement; Bivalvia; Crassostrea gigas.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00043/15460/12835.pdf
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Mise au point d'outils de gestion des gamètes mâles d'huîtres creuses Crassostrea gigas diploïdes et tétraploïdes à des fins de conservation et de diffusion ArchiMer
Brizard, Raphael; Boudry, Pierre; Haffray, Patrick; Labbe, C.; Suquet, Marc; Robert, Rene; Masse, G.; Pepin, Jean-francois; Saulnier, Denis.
In order to improve oyster culture, many research programs try and propose new methods aiming at improving genetically the product performances and quality (selection, polyploidisation). As for terrestrial animal production, the continuity of the different works as well as the spreading of the genetical progress would be made easier by the development of conservation techniques of the selected genotypes. Cryopreservation, which is often used in selection programs of terrestrial animals or fish farming species, is not always available for bivalves. This technique would enable the conservation of the gametes of individuals selected for their high growth potential, their resistance to diseases and/or their quality for a smaller cost and less risks than the...
Tipo: Text Palavras-chave: Tetraploïde; Diploïde; Concervation; Spermatozoides; Cryoconservation; Génétique; Crassostrea gigas; Huîtres.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3339.pdf
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Procédures d'échantillonnage pour la conservation des espèces aquacoles dans la Cryobanque Nationale ArchiMer
Vandeputte, Marc; Labbe, Catherine; Suquet, Marc; Fauvel, Christian; Goardon, Lionel; Duclos, Delphine; Danchin-burge, Coralie; Vergnet, Alain; Rault, Paul; Degremont, Lionel; Benabdelmouna, Abdellah; Brizard, Raphael; Haffray, Pierrick.
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20975/18597.pdf
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Projet CRYOYSTER: Optimisation, standardisation et validation de la congélation de laitance d'huître creuse Crassostrea gigas à des fins de conservation et de diffusion génétique - Appel d'offre de l'OFIMER du 8 Novembre 2001 - Rapport final ArchiMer
Brizard, Raphael; Bernardi, Maud; Boudry, Pierre; Haffray, Pierrick; Labbe, Catherine; Maisse, Gérard; Maurouard, Elise; Robert, Rene; Roger, Jean-luc.
Afin d'appuyer la filière ostréicole, les programmes de recherche actuels visent à proposer des méthodes d'amélioration génétique des rendements et de la qualité du produit (sélection, polyploïdisation). A l'image des productions animales terrestres, la continuation de ces travaux et la diffusion du progrès génétique dans la filière seraient grandement facilitée par le développement de techniques de conservation des génotypes sélectionnés. La cryopréservation qui est utilisée fréquemment dans les programmes de sélection des animaux terrestres et des espèces piscicoles n'est toujours pas disponible pour les mollusques bivalves. Cette technique présente les atouts qui peuvent pourraient satisfaire les différents acteurs de la filière ostréicole car elle...
Tipo: Text Palavras-chave: Congélation sperme; Standardisation; Procédure; Ecloserie expérimentale; Fécondation; Cryopréservation; Sperme cryopréservé.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00049/16056/13516.pdf
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Rapport de mission à Florianopolis 20-27/10/2002 (Etat de Santa Catarina, Brésil) ArchiMer
Le Moine, Olivier; Brizard, Raphael; Jeanneret, Helene; Gonzales, Ramiro; Riou, Philippe.
Cette mission a été réalisée sous l'égide de la Mairie de Florianopolis, capitale de l'état de Santa Catarina. Cet état est considéré comme un site pilote au niveau du Brésil pour le développement de l'aquaculture. Cette mission s'inscrit dans une coopération Franco-Brésilienne débutée en 2001 avec la visite de Madame le Maire de Florianopolis à Marennes-Oléron, suivie d'une autre mission Brésilienne et du séjour de 3 ostréiculteurs de Florianopolis. Une mission Française a également eu lieu en octobre 2001 à Florianopolis lors de la fête nationale de l'huître "Fenaostra". Cette manifestation regroupe chaque année des participants de nombreux pays étrangers. En 2002 elle a accueilli 60000 visiteurs en 4 jours, et a donné lieu à une vingtaine...
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Ano: 2002 URL: http://archimer.ifremer.fr/doc/00413/52484/53273.pdf
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Sediment accumulation in earthen shrimp ponds ArchiMer
Lemonnier, Hugues; Brizard, Raphael; Herlin, Jose; Mugnier, Chantal.
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Ano: 2001 URL: http://archimer.ifremer.fr/doc/00354/46556/46367.pdf
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Setting of a procedure for experimental incubation of Pacific oyster (Crassostrea gigas) embryos ArchiMer
Suquet, Marc; Amourda, Christopher; Mingant, Christian; Queau, Isabelle; Lebrun, Luc; Brizard, Raphael.
Standardized experimental protocols designed to study Pacific oyster (Crassostrea gigas) gamete quality have not been previously published. Gamete sampling variations and confounding effect of interacting factors result in large variations between replicates, decreasing the effects of the studied factors. This work aims at defining a standardized procedure for incubation of oyster embryos designed for experimental purposes. In a first phase, four experiments were developed to improve embryo sampling and handling. They showed that a minimum of 50 embryos must be counted to decrease the variation between counting replicates. For reliable results, sampling must be carried out from 0 to 7 min after a careful agitation of seawater containing embryos...
Tipo: Text Palavras-chave: Crassostrea gigas; Pacific oyster; Experimental; Embryo; Incubation.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3593.pdf
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Study of genetic damage in the japanese oyster induced by an environmentally-relevant exposure to diuron: evidence of vertical transmission of dna damage ArchiMer
Barranger, Audrey; Akcha, Farida; Rouxel, Julien; Brizard, Raphael; Maurouard, Elise; Pallud, Marie; Menard, Dominique; Tapie, N.; Budzinski, Helene; Burgeot, Thierry; Benabdelmouna, Abdellah.
Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland. The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg...
Tipo: Text Palavras-chave: Herbicide; Diuron; Oyster; Genotoxicity; Comet assay; Cytogenetics.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00162/27311/25541.pdf
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Toxic factors of Vibrio strains pathogenic to shrimp ArchiMer
Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B.
Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida...
Tipo: Text Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf
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Validation in commercial conditions of the response to selection of the European flat oyster Ostrea edulis for resistance to Bonamia ostreae ArchiMer
Bedier, Edouard; Langlade, Aime; Angeri, Stéphane; Brizard, Raphael; Nerlovic, V.; Glize, Philippe; Haffray, Philippe.
Since the outbreaks of the parasitosis with Marteilia refringens and Bonamia ostreae in the last 60's, the French production of the European flat oyster dropped from 20000 mT to less than 1500 mT currently, leaving the French shellfish farming in situation of virtually monoculture of the Pacific cupped oyster Crassostrea gigas. To sustain this traditional culture, Ifremer has developed, at the experimental level, a genetic selection for a better tolerance to Bonamia ostreae which led to an improvement of survivals. The transfer of these results to the business scale requires the control of the reproduction in hatchery and the adaptation of the cycle of production to the constraints of seedlings in deep water as practised in Brittany (France). That's why a...
Tipo: Text Palavras-chave: Selection; Genetic; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3313.pdf
Registros recuperados: 19
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