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Complementary approaches to diagnosing marine diseases: a union of the modern and the classic ArchiMer
Burge, Colleen A.; Friedman, Carolyn S.; Getchell, Rodman; House, Marcia; Lafferty, Kevin D.; Mydlarz, Laura D.; Prager, Katherine C.; Sutherland, Kathryn P.; Renault, Tristan; Kiryu, Ikunari; Vega-thurber, Rebecca.
Linking marine epizootics to a specific etiology is notoriously difficult. Recent diagnostic successes show that marine disease diagnosis requires both modern, cutting-edge technology (e.g. metagenomics, quantitative realtime PCR) and more classic methods (e.g. transect surveys, histopathology and cell culture). Here, we discuss how this combination of traditional and modern approaches is necessary for rapid and accurate identification of marine diseases, and emphasize how sole reliance on any one technology or technique may lead disease investigations astray. We present diagnostic approaches at different scales, from the macro (environment, community, population and organismal scales) to the micro (tissue, organ, cell and genomic scales). We use disease...
Tipo: Text Palavras-chave: Marine disease; Aetiology; Diagnostics; Marine epizootics.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00314/42536/41911.pdf
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First evaluation of resistance to both a California OsHV-1 variant and a French OsHV-1 microvariant in Pacific oysters ArchiMer
Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Dégremont, Lionel; Burge, Colleen A.; Mancilla Cortez, Daniel; Friedman, Carolyn S.; Fleener, Gary B.; Dumbauld, Brett R.; Langdon, Chris.
Background Variants of the Ostreid herpesvirus 1 (OsHV-1) cause high losses of Pacific oysters globally, including in Tomales Bay, California, USA. A suite of new variants, the OsHV-1 microvariants (μvars), cause very high mortalities of Pacific oysters in major oyster-growing regions outside of the United States. There are currently no known Pacific oysters in the United States that are resistant to OsHV-1 as resistance has yet to be evaluated in these oysters. As part of an effort to begin genetic selection for resistance to OsHV-1, 71 families from the Molluscan Broodstock Program, a US West Coast Pacific oyster breeding program, were screened for survival after exposure to OsHV-1 in Tomales Bay. They were also tested in a quarantine laboratory in...
Tipo: Text Palavras-chave: Ostreid herpesvirus 1; Crassostrea gigas; Heritability; Breeding.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00598/70974/69206.pdf
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