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Registros recuperados: 57
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A novel "Integrated Biomarker Response" calculation based on reference deviation concept ArchiMer
Sanchez, Wilfried; Burgeot, Thierry; Porcher, Jean-marc.
Multi-biomarker approaches are used to assess ecosystem health and identify impacts of environmental stress on organisms. However, exploration of large datasets by environmental managers represents a major challenge for regulatory application of this tool. Several integrative tools were developed to summarize biomarker responses. The aim of the present paper is to update calculation of the "Integrated Biological Response" (IBR) described by Beliaeff and Burgeot (Environ Toxicol Chem 21:1316-1322, 2002) to avoid weaknesses of this integrative tool. In the present paper, a novel index named "Integrated Biological Responses version 2" based on the reference deviation concept is presented. It allows a clear discrimination of sampling sites as for the IBR, but...
Tipo: Text Palavras-chave: Biomarkers; Data analysis; Graphic tools; Three-spined stickleback.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00139/25069/23191.pdf
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Assessment of contaminant concentrations in sediments, fish and mussels sampled from the North Atlantic and European regional seas within the ICON project ArchiMer
Robinson, Craig D.; Webster, Lynda; Martinez-gomez, Concepcion; Burgeot, Thierry; Gubbins, Matthew J.; Thain, John E.; Vethaak, A. Dick; Mcintosh, Alistair D.; Hylland, Ketil.
Understanding the status of contaminants in the marine environment is a requirement of European Union Directives and the Regional Seas Conventions, so that measures to reduce pollution can be identified and their efficacy assessed. The international ICON workshop (Hylland et al., in this issue) was developed in order to test an integrated approach to assessing both contaminant concentrations and their effects. This paper describes and assesses the concentrations of trace metals, polycyclic aromatic hydrocarbons, and polychlorinated biphenyls in sediments, mussels, and fish collected from estuarine, coastal and offshore waters from Iceland to the Mediterranean Sea. For organic contaminants, concentrations progressively increased from Iceland, to the...
Tipo: Text Palavras-chave: Integrated assessment; ICES; North Atlantic; North Sea; Baltic Sea; Mediterranean Sea; Polycyclic aromatic hydrocarbons (PAHs); Polychlorinated biphenyls (PCBs); Trace metals; Environmental monitoring.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00324/43520/43051.pdf
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Bioindicators of pollutant exposure in the northwestern Mediterranean sea ArchiMer
Burgeot, Thierry; Bocquene, Gilles; Porte, C; Dimeet, Joel; Santella, Rm; Garcia De La Parra, L.m.; Pihol-leszkowicz, A.; Raoux, C.; Galgani, Francois.
Several bioindicators were used to evaluate the biological and genotoxic effects of marine pollutants near large coastal cities in the northwestern part of the Mediterranean Sea. Three target species of teleosts were selected: red mullet Mullus barbatus and 2 types of comber (Serranus hepatus and S. cabrilla). Induction of ethoxyresorufin-O-deethylase (EROD) activity specific for polycyclic aromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) was measured in the livers of the fish, and inhibition of acetylcholinesterase (AChE) by organophosphorus insecticides and carbamates was measured in their muscle tissues. Maximal EROD activities (16.8 +/- 2.7 to 19.4 +/- 4.2 pmol min(-1) mg protein(-1)) recorded in red muller near Barcelona (Spain),...
Tipo: Text Palavras-chave: EROD; GST; AChE; Antioxidant enzymes; Biomonitoring; DNA adducts; PAH; Mullus barbatus.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00183/29410/27779.pdf
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Biological effects of the "Erika" oil spill on the common mussel (Mytilus edulis) ArchiMer
Bocquene, Gilles; Chantereau, Sebastien; Clerendeau, Christelle; Beausir, Emilie; Menard, Dominique; Raffin, Bernard; Minier, Christophe; Burgeot, Thierry; Leszkowicz, Annie Pfohl; Narbonne, Jean-françois.
A 3-year survey was made of several biological markers in mussels (Mytilus edulis) exposed in situ to the oil that came ashore after the wreck of the "Erika" tanker on the Brittany (France) coast in December 1999. The mussel response was assessed using a set of 7 biomarkers, most of them related to the metabolism of organic contaminants. After a series of validation tests, data was evaluated for only 5 biomarkers: acety1cholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT), malondialdehyde (MDA) and deoxyribonucleic acid (DNA) adducts. No significant reductions in GST or CAT levels were observed, levels of DNA adducts and MDA were high during the 6 months immediately following the accident and levels of AChE were significantly lower during...
Tipo: Text Palavras-chave: Mytilus edulis; Mussel; Biomarkers; Monitoring; Survey; PAH; Polycyclic aromatic hydrocarbon; Oil spill.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-403.pdf
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Caractérisation des facteurs de risques associés au mortalités estivales - Synthèse du thème 3 - Risque associé au stress environnemental - 2002-2005 ArchiMer
Burgeot, Thierry; Gagnaire, Beatrice; Renault, Tristan; Haure, Joel; Moraga, D.; David, E.; Boutet, L.; Sauriau, P.g.; Malet, Nathalie; Bouchet, V.; Lapegue, Sylvie; Bouilly, Karine; Le Moullac, Gilles; Arzul, Genevieve; Knoery, Joel; Quiniou, Francoise; Bacher, Cedric; Soletchnik, Patrick.
Les mortalités d'huîtres observées sur les côtes françaises apparaissent principalement à proximité du sédiment et pendant la gamétogenèse. Les interactions de facteurs environnementaux sur les écosystèmes conchylicoles comme la température, les apports des bassins versants et le compartiment sédimentaire illustrent un risque de mortalité lié à différentes sources de stress (Thème 1). Les variations de la qualité de la ressource trophique pendant l'effort de gamétogenèse favorise une baisse de l'énergie disponible (Thème 2) et contraint l'organisme fragilisé à fournir un effort d'adaptation (Fent 2004). L'apparition d'un stress pendant cette période sensible des huîtres pourrait constituer un facteur aggravant de mortalité. L'objectif de cette étude est...
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Ano: 2006 URL: http://archimer.ifremer.fr/doc/00304/41538/40746.pdf
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Changes in the sediment trace metal contamination after the commissioning of a municipal wastewater treatment plant in the Souss estuary (South Morocco) ArchiMer
Moukrim, A; Chiffoleau, Jean-francois; Cheggour, M; Burgeot, Thierry.
Monthly samples of sediment were collected from the Souss estuary, to determine the changes which have occurred in the concentrations of ten metals between 2001 when the low estuary was receiving the wastewaters from Agadir city and 2003 after stopping the sewage dumping. The annual contents of Cu, Fe, Zn (in 2001) and those of Cr, Cu, Fe, Mn, Ni, Pb, and Zn (in 2003) showed significant correlations with those of Al, indicating that fluctuations of their concentrations were only due to those of the aluminosilicate fraction of the clayey sediment. After normalization of these metallic concentrations in relation to those of Al, the contents of the different metals showed insignificant correlations between both years, except for Zn which significantly dropped...
Tipo: Text Palavras-chave: Souss estuary; Wastewater; Sediment; Metal contamination.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-5923.pdf
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Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects ArchiMer
Larcher, Thibaut; Perrichon, Prescilla; Vignet, Caroline; Ledevin, Mireille; Le Menach, Karyn; Lyphout, Laura; Landi, Laure; Clerandeau, Christelle; Lebihanic, F.; Menard, Dominique; Burgeot, Thierry; Budzinski, Helene; Akcha, Farida; Cachot, J.; Cousin, Xavier.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All...
Tipo: Text Palavras-chave: Polycyclic aromatic hydrocarbon; Zebrafish; Carcinogenesis; Genotoxicity; Neoplasia; Carcinoma; Toxicological pathology.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00242/35329/34108.pdf
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Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: implication of reactive oxygen species production ArchiMer
Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida.
Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore...
Tipo: Text Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf
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Conditions d'apparition de densités accrues de dinoflagellés Dinophysis dans le pertuis d'Antioche et les eaux adjacentes. Campagnes Dinopertuis 89 ArchiMer
Delmas, Daniel; Herbland, Alain; Maestrini, Serge; Mornet, Francoise; Morand, Philippe; Spano, Anna-maria; Burgeot, Thierry; Margat, Sylvie; Charpentier, Gabriel; Fillon, Alain; Leguay, Didier; Thomas, Gerard; Bacher, Cedric; Heral, Maurice; Prou, Jean.
Environmental and species features which lead to increases in cell density of the D.S.P. Dinoflagellate Dinophysis in inshore and offshore waters of the île de Ré - île d'Oléron area, France (cruises "Dinopertuis89"). Four weekly sampling series have been carried out, within 10 May – 31 May 1989, at 24 stations located from inshore to offshore waters of the "île de Ré" - "île d’Oléron" area, with the aim to investigate (i) whether Dinophysis increases in cell density occur first in offshore waters and then, eventually, are pushed to the coast, or develop simultaneously in the whole area, and (ii) whether there are some environmental conditions which are related to Dinophysis blooms. Depths of sampling have been stated on the bas is of STD profiles. All...
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Ano: 1990 URL: http://archimer.ifremer.fr/doc/00174/28532/26916.pdf
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Contaminant exposure and ecotoxicological impacts in estuaries ArchiMer
Burgeot, Thierry; Gagne, Francois.
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00124/23568/22482.pdf
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Défi MOREST - Mortalités etivales de l'huître creuse Crassostrea gigas - Caractérisation des facteurs de risques associés aux mortalités estivales - Synthèse du thème 4B - Caractérisation phénotypique des souches R Résistantes et S Susceptibles à la mortalité estivale. (La Rochelle 14-15 mars 2006) ArchiMer
Huvet, Arnaud; Royer, J.; Moal, J.; Burgeot, Thierry; Lapègue, Sylvie; Boulo, Viviane; Nicolas, Jean-louis; Lambert, C..
Les résultats du programme MOREST démontrent l'existence d'une composante génétique à la survie estivale du naissain et nous permettent de disposer aujourd'hui d'huTtres « résistantes» (R) et « susceptibles» (S) aux mortalités estivales (Dégremont et aL, 2002, 2005). La forte héritabilité estimée face à un tel système multifactoriel permet de suspecter que le caractère sélectionné puisse être gouverné par un ou un trés petit nombre de gènes sur une région chromosomique donnée. D'après le modèle d'interaction suspecté entre l'environnement, l'huître et des pathogènes opportunistes, une hiérarchie des facteurs, conduisant aux mortalités, permet de montrer qu'aucun d'entre eux ne peut provoquer à lui seul les mortalités mais que leur combinaison est...
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Ano: 2006 URL: http://archimer.ifremer.fr/doc/00306/41706/40917.pdf
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Descriptif des stations d'épuration ayant un rejet à la mer. Proposition d'un système de contrôle définissant la qualité du milieu marin ArchiMer
Burgeot, Thierry; Fillon, Alain.
La surveillance et le suivi de la qualité du milieu est une des missions essentielles confiées au Laboratoire CSRU. Au Laboratoire de La Rochelle, le problème de l'assainissement et en particulier des rejets en mer, dans les zones conchylicoles des effluents des stations d'épuration fait l'objet, depuis plusieurs années, d'un contrôle régulier. Chaque contrôleur assure cette tâche par des prélèvements, effectués dans les différentes stations de leur circonscription.
Tipo: Text Palavras-chave: Station épuration; Rejet; Milieu marin; Effluent; Système contrôle.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/00149/26029/24130.pdf
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DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas ArchiMer
Hylland, Ketil; Skei, Bjorn Borge; Brunborg, Gunnar; Lang, Thomas; Gubbins, Matthew J.; Le Goff, Jeremie; Burgeot, Thierry.
Dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrations. DNA strand breaks were at background levels in dab from the two Iceland locations and from the Dogger Bank. The highest levels were observed in dab from the Firth of Forth, Ekofisk and the German Bight. Hepatic DNA adducts in dab were highest at Ekofisk, in the Baltic and Dogger Bank, below detection limit in dab from Iceland and low in dab from the Firth of Forth and German Bight. There was large variation in DNA strand breaks between locations and...
Tipo: Text Palavras-chave: ICON; Comet; DNA adducts; Genotoxicity; Haddock; Dab; European seas.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00309/42064/44971.pdf
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Effect of in vivo pesticide exposure and injection of bacteria on immune gene expression in the Pacific oyster, Crassostrea gigas ArchiMer
Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry.
Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and...
Tipo: Text Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf
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Effects of increasing temperatures on biomarker responses and accumulation of hazardous substances in rope mussels (Mytilus galloprovincialis) from Bizerte lagoon ArchiMer
Kamel, Naouel; Burgeot, Thierry; Banni, Mohamed; Chalghaf, Mohamed; Devin, Simon; Minier, Christophe; Boussetta, Hamadi.
This study examined the influence of increasing temperatures in spring and summer on biochemical biomarkers in Mytilus galloprovincialis mussels sampled from Bizerte lagoon (northern Tunisia). Spatial and seasonal variations in a battery of seven biomarkers were analyzed in relation to environmental parameters (temperature, salinity and pH), physiological status (condition and gonad indexes), stress on stress (SoS) and chemical contaminant levels (heavy metals, PAHs and PCBs) in digestive glands. Integrated Biological Response (IBR) was calculated using seven biomarkers (AChE, BPH, MXR, GST, CAT, MDA and MT). Seasonal variations in biological response were determined during a critical period between spring and summer at two sites, where chemical...
Tipo: Text Palavras-chave: Biomarkers; Mussels; Lagoon biomonitoring; Integrated biomarker response.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00171/28224/26471.pdf
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Effects of water accommodated fractions of crude oils and diesel on a suite of biomarkers in Atlantic cod (Gadus morhua) ArchiMer
Holth, T. F.; Eidsvoll, D. P.; Farmen, E.; Sanders, M. B.; Martinez-gomez, C.; Budzinski, H.; Burgeot, Thierry; Guilhermino, L.; Hylland, K..
The aim of this study was to characterise concentration- and time-dependent responses in juvenile Atlantic cod (Gadus morhua) following exposure for one and three weeks to the water-soluble fraction (WAF) of three weathered oils: Arabian Light crude oil (ALC), North Sea crude oil (NSC) and ship-diesel. The sum of polycyclic aromatic hydrocarbons (PAH) in water was highest after one week of exposure and within environmentally relevant concentrations. PAH metabolites in bile confirmed exposure to and uptake of PAHs. Hepatic cytochrome P450 1A (CYP1A) gene expression (here and later the phrase gene expression is used synonymously to gene transcription, although it is acknowledged that gene expression is also regulated by, e.g., translation and protein...
Tipo: Text Palavras-chave: Atlantic cod; Biomarkers; Weathered crude oil; Ship-diesel; Water accommodated fraction; Time-course.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00190/30131/28593.pdf
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Effets de la contamination chimique : des organismes en danger ? ArchiMer
Poisson, Emeline; Fisson, Cédric; Amiard-triquet, Claude; Burgeot, Thierry; Couteau, Jérôme; Dur, Gael; Durand, Fabrice; Forget-leray, Joelle; Letendre, Julie; Souissi, Sami; Xuereb, Benoit.
Le présent fascicule a pour objectif d’apporter les éléments de réponse disponibles pour l’estuaire de la Seine sur ce questionnement. Dans la première partie écotoxicologie. Les effets de la contamination chimique à différents niveaux d’organisation biologique, sur les organismes présents dans l’estuaire de la Seine, seront présentés dans la deuxième partie puis synthétisés dans notion d’analyse du risque environnemental.
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00319/43019/42534.pdf
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Effets d'un mélange de pesticides sur les hémocytes d'huître creuse, Crassostrea gigas. ArchiMer
Gagnaire, Beatrice; Geret, Florence; Haure, Joel; Martin, Jean-louis; Burgeot, Thierry; Thomas-guyon, Hélène; Renault, Tristan.
Shellfish industry is often realised in estuarine areas, subjected to pollutions due to agricultural activities. The harmful effects of pesticides on animals inhabiting these estuarine zones are poorly documented. Bivalve molluscs represent an interesting model because they are sedentary and they filter water intensively. Effects of pesticides on haemocytes functions were tested in Pacific oyster, Crassostrea gigas and analysed by flow cytometry. A combination of pesticide at environmental concentrations was tested in vivo. Animals used corresponded to two genetically selected populations produced in hatchery.
Tipo: Text Palavras-chave: Haemocytes; Flow cytometry; Pesticides; Crassostrea gigas.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-924.pdf
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Environmental stress in the pacific oyster Crassostrea gigas during gametogenesis : role of herbicides? ArchiMer
Burgeot, Thierry; Quirion, Sophie; Quiniou, Francoise; Knoery, Joel; Soletchnik, Patrick; Gagnaire, Beatrice; Renault, Tristan; Osada, M.; Geret, F.; Communal, P. Y.; Jadas-hecart, A.; Gagne, F..
In the Atlantic French coast, significant mortalities were observed in oyster communities during gametogenesis. These deaths possibly result from the cumulative effects of various stressors such as seasonal temperature variations, pesticide application and the release of contaminants from the resuspension of sediments. A field survey, based on aquaculture conditions at 15 and 70 cm from the sediment bed with genetically-resistant (R) and -sensitive (S) lines, showed a stress condition. Significant weight loss was observed during May 14th through June 10th for both R and S lines. On one hand, the levels of the egg yolk vitellin in females were decreased at May 14th for both R and S lines. On the other hand, a significant increase in vitellin production was...
Tipo: Text Palavras-chave: Mortalities; Herbicides; Sediment; Biomarkers; Gametogenesis; Crassostrea gigas; Pacific oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3384.pdf
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Essai de décontamination à l'échelle professionnelle de moules rendues toxiques par le phytoplancton ArchiMer
Masson, Daniel; Ratiskol, Gilles; Burgeot, Thierry; Margat, Sylvie.
Les secteurs conchylicoles atteints par les efflorescences phytoplanctoniques toxiques sont fermés par l'Administration dès que le taux de contamination devient dangereux pour la consommation humaine. Cette fermeture peut durer plusieurs mois empêchant les conchyliculteurs de commercialiser leurs produits, ce qui a des conséquences économiques parfois graves. Il n'existe jusqu'à présent aucune possibilité technique de décontaminer ces coquillages en grande quantité et dans des délais économiquement viables. L'étude entreprise visait à mettre au point une technique permettant aux professionnels de décontaminer des coquillages rapidement (quinze jours au moins) en milieu contrôlé (bassins). L'hypothèse testée était que des coquillages placés dans un milieu...
Tipo: Text Palavras-chave: Coquillages; Décontamination; Toxicité; Phytoplancton.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00134/24499/22527.pdf
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