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Algogroup: Towards a Shared Vision of the Possible Deployment of Algae to Biofuels ArchiMer
Montagne, Xavier; Porot, Pierre; Aymard, Caroline; Querleu, Cecile; Bouter, Anne; Lorne, Daphne; Cadoret, Jean-paul; Lombaert-valot, Isabelle; Petillon, Odile.
A strong interest has been focused from several years on the algae pathway for energy production, especially for transportation fuels called third generation biofuel or G3 biofuel, and mainly from microalgae route, considering it could be a high potential alternative strategy for renewable energy and fuel production. Algae, and especially microalgae, present significant advantages compared with land resources, such as much higher productivity and lack of competition with food applications. Nevertheless, based on current knowledge, the production of an algae biomass for energy remains a difficult target to reach, due to the numerous existing hurdles such as the energetic yield and the economic positioning, without neglecting the environmental and societal...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00174/28565/26941.pdf
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Antiproliferative Activity of Cyanophora paradoxa Pigments in Melanoma, Breast and Lung Cancer Cells ArchiMer
Baudelet, Paul-hubert; Gagez, Anne-laure; Berard, Jean-baptiste; Juin, Camille; Bridiau, Nicolas; Kaas, Raymond; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent.
The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 mu g.mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 mu g.mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of...
Tipo: Text Palavras-chave: Pigments; Microalgae; Cyanophora paradoxa; Melanoma; Cancer; Apoptosis; Zeaxanthin; Cryptoxanthin.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00177/28818/27289.pdf
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Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts ArchiMer
Pasquet, Virginie; Morisset, Perrine; Ihammouine, Said; Chepied, Amandine; Aumailley, Lucie; Berard, Jean-baptiste; Serive, Benoit; Kaas, Raymond; Lanneluc, Isabelle; Thiery, Valerie; Lafferriere, Mathieu; Piot, Jean-marie; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at...
Tipo: Text Palavras-chave: Pigments; Microalgae; Dunaliella; Violaxanthin; Carotenoid; Cancer; Apoptosis.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15142/12575.pdf
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Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups ArchiMer
Serive, Benoit; Nicolau, Elodie; Berard, Jean-baptiste; Kaas, Raymond; Pasquet, Virginie; Picot, Laurent; Cadoret, Jean-paul.
Phytoplankton, with an estimated 30 000 to 1 000 000 species clustered in 12 phyla, presents a high taxonomic and ecophysiological diversity, reflected by the complex distribution of pigments among the different algal classes. High performance liquid chromatography is the gold standard method for qualitative and quantitative analysis of phytoplankton pigments in seawater and culture samples, but only a few pigments can be used as robust chemotaxonomic markers. A major challenge is thus to identify new ones, characteristic of a strain, species, class or taxon that cannot be currently identified on the basis of its pigment signature. Using an optimized extraction process coupled to a HPLC de-replication strategy, we examined the pigment composition of 37...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48416/48627.pdf
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Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea ArchiMer
Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul.
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins...
Tipo: Text Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf
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Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle ArchiMer
Carrier, Gregory; Garnier, Matthieu; Le Cunff, Loic; Bougaran, Gael; Probert, Ian; De Vargas, Colomban; Corre, E; Cadoret, Jean-paul; Saint-jean, Bruno.
The applied exploitation of microalgae cultures has to date almost exclusively involved the use of wild type strains, deposited over decades in dedicated culture collections. Concomitantly, the concept of improving algae with selection programs for particular specific purposes is slowly emerging. Studying since a decade an economically and ecologically important haptophyte Tisochrysis lutea (Tiso), we took advantage of the availability of wild type (Tiso-Wt) and selected (Tiso-S2M2) strains to conduct a molecular variations study. This endeavour presented substantial challenges: the genome assembly was not yet available, the life cycle unknown and genetic diversity of Tiso-Wt poorly documented. This study brings the first molecular data in order to set up...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00175/28662/27089.pdf
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Crassostrea gigas ferritin: cDNA sequence analysis for two heavy chain type subunits and protein purification ArchiMer
Durand, Jean-pierre; Goudard, Françoise; Pieri, Jacques; Escoubas, Jean-michel; Schreiber, Nathalie; Cadoret, Jean-paul.
Ferritin has been shown as being the principal iron storage in the majority of living organisms. In marine species, ferritin is also involved in high-level accumulation of Po-210. As part of our work on the investigation of these radionuclides' concentration in natural environment, ferritin was searched at the gene and protein level. Ferritin was purified from the visceral mass of the oyster Crassostrea gigas by ion-exchange chromatography and HPLC. SDS-PAGE revealed one band of 20 kDa. An Expressed Sequence Tag (EST) library was screened and led to the identification of two complementary DNA (cDNA) involved in ferritin subunit expression. The complete coding sequences and the untranslated regions (UTRs) of the two genes were obtained and a 5' Rapid...
Tipo: Text Palavras-chave: Iron; IRE; EST; Oyster.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-693.pdf
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Digital expression profiling of novel diatom transcripts provides insight into their biological functions ArchiMer
Maheswari, Uma; Jabbari, Kamel; Petit, Jean-louis; Porcel, Betina M.; Allen, Andrew E.; Cadoret, Jean-paul; De Martino, Alessandra; Heijde, Marc; Kaas, Raymond; La Roche, Julie; Lopez, P.; Martin-jezequel, Veronique; Meichenin, Agnes; Mock, Thomas; Parker, Micaela Schnitzler; Vardi, Assaf; Armbrust, E. Virginia; Weissenbach, Jean; Katinka, Michael; Bowler, Chris.
Background: Diatoms represent the predominant group of eukaryotic phytoplankton in the oceans and are responsible for around 20% of global photosynthesis. Two whole genome sequences are now available. Notwithstanding, our knowledge of diatom biology remains limited because only around half of their genes can be ascribed a function based onhomology-based methods. High throughput tools are needed, therefore, to associate functions with diatom-specific genes. Results: We have performed a systematic analysis of 130,000 ESTs derived from Phaeodactylum tricornutum cells grown in 16 different conditions. These include different sources of nitrogen, different concentrations of carbon dioxide, silicate and iron, and abiotic stresses such as low temperature and low...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00017/12810/9758.pdf
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Effects of blue light on the biochemical composition and photosynthetic activity of Isochrysis sp. (T-iso) ArchiMer
Marchetti, Julie; Bougaran, Gael; Jauffrais, Thierry; Lefebvre, Sebastien; Rouxel, Catherine; Saint-jean, Bruno; Lukomska, Ewa; Robert, Rene; Cadoret, Jean-paul.
In aquaculture, particularly in bivalve hatcheries, the biochemical composition of algal diets has a strong influence on larval and post-larval development. Biochemical composition is known to be related to culture conditions, among which light represents a major source of variation. The effects of blue light on biochemical composition and photosynthetic rate of Isochrysis sp. (T-iso) CCAP 927/14 were assessed in chemostat at a single irradiance (300 μmol photons m−2 s−1) and compared with white light. Two different dilution (renewal) rates were also tested: 0.7 and 0.2 d−1. Relative carbohydrate content was lower under blue light than under white light at both dilution rates, whereas chlorophyll a and photosynthesis activity were higher. In contrast,...
Tipo: Text Palavras-chave: Isochrysis; Blue light; Photosynthesis; Proximate composition; Chemostat.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00085/19599/17237.pdf
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Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea ArchiMer
Da Costa, Fiz; Le Grand, Fabienne; Quere, Claudie; Bougaran, Gael; Cadoret, Jean-paul; Robert, Rene; Soudant, Philippe.
Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates...
Tipo: Text Palavras-chave: Tisochrysis lutea; Nitrogen limitation; Growth phase; Flow cytometry; Fatty acids; Lipid classes.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00399/51074/51936.pdf
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Effects of nitrogen limitation on Dunaliella sp.–Alteromonas sp. interactions: from mutualistic to competitive relationships. ArchiMer
Le Chevanton, Myriam; Garnier, Matthieu; Lukomska, Ewa; Schreiber, Nathalie; Cadoret, Jean-paul; Saint-jean, Bruno; Bougaran, Gael.
Interactions between photosynthetic and non-photosynthetic microorganisms play an essential role in natural aquatic environments and the contribution of bacteria and microalgae to the nitrogen cycle can lead to both competitive and mutualistic relationships. Nitrogen is considered to be, with phosphorus and iron, one of the main limiting nutrients for primary production in the oceans and its availability experiences large temporal and geographical variations. For these reasons, it is important to understand how competitive and mutualistic interactions between photosynthetic and heterotrophic microorganisms are impacted by nitrogen limitation. In a previous study performed in batch cultures, the addition of a selected bacterial strain of Alteromonas sp....
Tipo: Text Palavras-chave: Interactions; Nitrogen limitation; Microalgae; Bacteria; Chemostat; Dissolved organic matter (DOM); Dunaliella.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00344/45529/45087.pdf
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Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure ArchiMer
Bougaran, Gael; Rouxel, Catherine; Dubois, Nolwenn; Kaas, Raymond; Grouas, Sophie; Lukomska, Ewa; Le Coz, Jean-rene; Cadoret, Jean-paul.
Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)
Tipo: Text Palavras-chave: Strain selection; Isochrysis; Neutral lipid; Ultra violet; Flux cytometry; Biofuel.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00085/19643/17418.pdf
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High-affinity nitrate/nitrite transporter genes (Nrt2) in Tisochrysis lutea: identification and expression analyses reveal some interesting specificities of Haptophyta microalgae ArchiMer
Charrier, Aurelie Georgette; Berard, Jean-baptiste; Bougaran, Gael; Carrier, Gregory; Lukomska, Ewa; Schreiber, Nathalie; Fournier, Flora; Charrier, Aurelie; Rouxel, Catherine; Garnier, Matthieu; Cadoret, Jean-paul; Saint-jean, Bruno.
Microalgae have a diversity of industrial applications such as feed, food ingredients, depuration processes and energy. However, microalgal production costs could be substantially improved by controlling nutrient intake. Accordingly, a better understanding of microalgal nitrogen metabolism is essential. Using in silico analysis from transcriptomic data concerning the microalgae Tisochrysis lutea, four genes encoding putative high-affinity nitrate/nitrite transporters (TlNrt2) were identified. Unlike most of the land plants and microalgae, cloning of genomic sequences and their alignment with complementary DNA (cDNA) sequences did not reveal the presence of introns in all TlNrt2 genes. The deduced TlNRT2 protein sequences showed similarities to NRT2...
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Ano: 2015 URL: https://archimer.ifremer.fr/doc/00253/36467/35044.pdf
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Identification of transcription factors involved in the phenotype of a domesticated oleaginous microalgae strain of Tisochrysis lutea ArchiMer
Thiriet-rupert, Stanislas; Carrier, Gregory; Trottier, Camille; Eveillard, D.; Schoefs, B.; Bougaran, Gael; Cadoret, Jean-paul; Chenais, B.; Saint-jean, Bruno.
Microalgae are an outstanding source of high value products with applications in food, feed or biofuel production. Among these promising organisms, the haptophyte Tisochrysis lutea is commonly used as a feed for shellfish and shrimps in aquaculture. Its capacity to produce high amounts of docosahexanoic acid and storage lipids is also of economic interest for nutrition and energy production. Consequently, understanding its lipid accumulation under nitrogen deprivation is of major interest. Here, we aimed to identify Transcription Factors (TFs) involved in the establishment of the mutant phenotype of the 2Xc1 strain of T. lutea, which accumulates double the quantity of storage lipids under nitrogen deprivation than the wild type strain (WTc1). Strains were...
Tipo: Text Palavras-chave: Algae; Carbohydrates; Gene co-expression network; Lipids; Transcription factor; Tisochrysis lutea.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00418/52973/53901.pdf
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Les microalgues, une voie d’avenir pour l’alimentation aquacole ? ArchiMer
Przybyla, Cyrille; Cadoret, Jean-paul; Calleja,.
Tipo: Text
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00608/71980/70678.pdf
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Microalgae, Functional Genomics and Biotechnology ArchiMer
Cadoret, Jean-paul; Garnier, Matthieu; Saint-jean, Bruno.
Microalgae have been studied for decades, but a new wave of research has recently begun as part of the search for renewable and sustainable energy sources. For economic optimization, microalgal biomass is being considered as a whole (main products and co-products) in an overall 'biorefinery' concept Applications of microalgae cover a broad spectrum, including the food and (livestock) feed industries, bio-energy, cosmetics, healthcare and environmental restoration or protection. In the field of biotechnology, the access to genomic data is playing a growing role. As the cost of sequencing strategies has fallen, studies of gene function at the transcript, protein and biosynthesis pathway levels have multiplied. Notably, sequencing and mass spectrometry...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00125/23602/21477.pdf
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Microwave-assisted extraction of phycobiliproteins from Porphyridium purpureum ArchiMer
Juin, Camille; Cherouvrier, Jean-rene; Thiery, Valerie; Gagez, Anne-laure; Berard, Jean-baptiste; Joguet, Nicolas; Kaas, Raymond; Cadoret, Jean-paul; Picot, Laurent.
In the present study, microwave-assisted extraction was first employed to extract the phycobiliproteins of Porphyridium purpureum (Pp). Freeze-dried Pp cells were subjected to microwave-assisted extraction (MAE) to extract phycoerythin (PE), phycocyanin (PC), and allophycocyanin (APC). MAE combined reproducibility and high extraction yields and allowed a 180- to 1,080-fold reduction of the extraction time compared to a conventional soaking process. The maximal PE extraction yield was obtained after 10-s MAE at 40 A degrees C, and PE was thermally damaged at temperatures higher than 40 A degrees C. In contrast, a flash irradiation for 10 s at 100 A degrees C was the best process to efficiently extract PC and APC, as it combined a high temperature necessary...
Tipo: Text Palavras-chave: Allophycocyanin; MAE; Microalgae; Microwave; Phycobiliprotein; Phycocyanin; Phycoerythrin; Porphyridium; Thylakoid.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00253/36439/35169.pdf
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Mise au point de méthodes de manipulation embryonnaire de mollusques bivalves. Application en génétique et pathologie infectieuse ArchiMer
Cadoret, Jean-paul.
Après vingt ans de programmes pivilégiant les méthodes d'élevage, les recherches concernant l'aquaculture, évoluent maintenant vers les domaines de la pathologie infectieuse et la génétique. La maîtrise des productions larvaires a en effet favorisé l'étude de la reproduction et de l'embryologie, et permet désormais d'aborder des travaux expérimentaux sur les différents stades embryonnaires. La polyploïdisation d'oeufs de moules ou d'huîtres a d'abord été envisagée par l'application d'un procédé d'électrofusion. Des expérimentations à grande échelle ont permis l'obtention d'embryons triploïdes (55% chez les huîtres et 36% chez les moules) ainsi que tétraploïdes (20% chez les huîtres et 26% chez les moules). La microinjection de spermatozoïdes a permis de...
Tipo: Text Palavras-chave: Mollusques; Bivalves; Polyploïdie; Electrofusion; Microinjection; Fécondation assistée; Rickettsies; Embryoculture; Trasfert de gène; B-galactosidase; Résistance; Pathologie; Génétique.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00440/55127/56585.pdf
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N-Glycans of Phaeodactylum tricornutum Diatom and Functional Characterization of Its N-Acetylglucosaminyltransferase I Enzyme ArchiMer
Baiet, Berengere; Burel, Carole; Saint-jean, Bruno; Louvet, Romain; Menu-bouaouiche, Laurence; Kiefer-meyer, Marie-christine; Mathieu-rivet, Elodie; Lefebvre, Thomas; Castel, Helene; Carlier, Aude; Cadoret, Jean-paul; Lerouge, Patrice; Bardor, Muriel.
N-Glycosylation, a major co- and post-translational event in the synthesis of proteins in eukaryotes, is unknown in aquatic photosynthetic microalgae. In this paper, we describe the N-glycosylation pathway in the diatom Phaeodactylum tricornutum. Bio-informatic analysis of its genome revealed the presence of a complete set of sequences potentially encoding for proteins involved in the synthesis of the lipid-linked Glc(3)Man(9)GlcNAc(2)-PP-dolichol N-glycan, some subunits of the oligosaccharyltransferase complex, as well as endoplasmic reticulum glucosidases and chaperones required for protein quality control and, finally, the alpha-mannosidase I involved in the trimming of the N-glycan precursor into Man-5 N-glycan. Moreover, one...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14184/11951.pdf
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Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries ArchiMer
Marchetti, Julie; Bougaran, Gael; Le Dean, Loic; Megrier, Cyril; Lukomska, Ewa; Kaas, Raymond; Olivo, Erell; Baron, Regis; Robert, Rene; Cadoret, Jean-paul.
Optimal culture conditions were investigated for the continuous culture of Isochrysis affinis galbana (T-Iso) by assessing the effects of irradiance, temperature, pH and nitrogen and phosphorus requirements. Assessment of the growth rate-irradiance relationship in a turbidostat experiment highlighted that an optimum irradiance efficiency for growth (E-mu) occurred at irradiance much lower than the saturating level. Furthermore, our modeling approach for productivity pointed out that an optimal set of irradiance and dilution rate could be achieved in order to maximize irradiance efficiency. Optimal conditions for growth were assessed for temperature, pH and irradiance by means of a factorial design experiment. The resulting model predicted that optimal...
Tipo: Text Palavras-chave: Isochrysis affinis galbana; Irradiance efficiency; Factor interaction; Nutrient limitation; Microalgae cultures.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00076/18736/16465.pdf
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