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Registros recuperados: 17
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Aggressiveness and physiological specialization of Septoria tritici Rob. isolates Scientia Agricola
Gieco,Jorge Omar; Dubcovsky,Jorge; Camargo,Luis Eduardo Aranha.
Pathogenicity tests verifying the behavior of Septoria tritici isolates should be considered as a priority in the selection of resistant wheat materials to this pathogen, since the aggressiveness of each isolate can vary significantly, causing problems in the evaluation and selection of resistant genotypes. The objective of this work was to determine whether physiologic specialization exists among Argentinean and American Septoria tritici isolates, through the analysis of their pathogenicity on cultivars and lines of bread wheat (Triticum aestivum L.). The experiments were carried out in Castelar-Argentina and in Davis-USA. In Castelar, a split plot design (n = 4) was used. The cultivars or lines were randomized in the plots and the isolates in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycosphaerella graminicola; Triticum aestivum; Leaf blotch; Vertical resistance; Horizontal resistance.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400010
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Aggressiveness between genetic groups I and II of isolates of Cercospora zeae-maydis Scientia Agricola
Mathioni,Sandra Marisa; Carvalho,; Brunelli,Kátia Regiane; Beló,André; Camargo,Luis Eduardo Aranha.
For many years, the gray leaf spot disease (GLS) caused by the fungus Cercospora zeae-maydis Tehon & Daniels, was not considered an important pathogen of maize (Zea mays, L.) in Brazil. However, the recent adoption of agronomical practices such as no-tillage and cultivation under central pivot irrigation systems increased the incidence and severity to the extent that GLS is now one of the most important diseases of maize. Isolates of C. zeae-maydis can be distinguished by two genetic groups (I and II) based on AFLP markers and on polymorphisms of the ITS and 5.8S rDNA regions. Until now, however, the biological implications of this distinction remain unclear. This study investigated whether isolates from the two genetic groups differ in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Disease resistance; Gray leaf spot; Genetic variability; Pathogenicity.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000600006
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Amplification of the cap20 pathogenicity gene and genetic characterization using different markers molecular in Colletotrichum gloeosporioides isolates BABT
Maciel,Danielli Barreto; Medeiros,Lílian Vieira de; Medeiros,Vivian Vieira de; Leão,Mariele Porto Carneiro; Camargo,Luis Eduardo Aranha; Oliveira,Neiva Tinti de.
Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers to cap20 gene were constructed using selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) with the Primer 3 program. The dendrograms analysis showed that the RFLP-ITS marker was more informative to separate the Colletotrichum sp, and that primer EI1 demonstrated greater genetic diversity. The amplification of the DNA of the Colletotrichum isolates to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cap20 gene; Colletotrichum gloeosporioides; Pathogenicity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600001
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An AFLP marker linked to the Pm-1 gene that confers resistance to Podosphaera xanthii race 1 in Cucumis melo Genet. Mol. Biol.
Teixeira,Ana Paula Matoso; Barreto,Fatima Aparecida da Silva; Camargo,Luis Eduardo Aranha.
Brazil produced 330,000 metric tons of melons in 2005, principally in the Northeast region where one of the most important melon pathogens is the powdery mildew fungus Podosphaera xanthii. The disease is controlled mainly by incorporating single dominant resistance genes into commercial hybrids. We report on linkage analysis of the Pm-1 resistance gene, introgressed from the AF125Pm-1 Cantalupensis Charentais-type breeding line into the yellow-fleshed melon (Group Inodorus) breeding line AF426-S by backcrossing to produce the resistant line AF426-R, and the amplified fragment length polymorphism (AFLP) marker M75/H35_155 reported to be polymorphic between AF426-S and AF426-R. Segregation analysis of M75/H35_155 using a backcross population of 143 plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP marker; Cucumis melo; Disease resistance; Linkage analysis; Powdery mildew.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300023
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Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus Scientia Agricola
Spadotti,David Marques de Almeida; Wassano,Débora Targino; Rezende,Jorge Alberto Marques; Camargo,Luis Eduardo Aranha; Inoue-Nagata,Alice Kazuko.
Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Potyvirus; Nucleotide sequence; Genetic diversity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000200187
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Breakdown of resistance in sweet pepper against Pepper yellow mosaic virus in Brazil Scientia Agricola
Gioria,Ricardo; Braga,Renato de Souza; Krause-Sakate,Renate; Roullier,Caroline; Rosa,Daniel Dias; Moura,Monika Fecury; Souza-Dias,José Alberto Caram de; Sawazaki,Haiko Enok; Camargo,Luis Eduardo Aranha; Rezende,Jorge Alberto Marques.
Plants of Capsicum annuum cv. Magali R, resistant to Pepper yellow mosaic virus (PepYMV), which showed severe yellow mosaic, leaf malformation and stunting were observed during the 2003/04 growing season in Lins, São Paulo State, Brazil. Potyvirus-like particles observed in leaf sap from infected plants under the electron microscope reacted with an antiserum against PepYMV in PTA-ELISA. In addition to C. annuum cv. Magali R, this potyvirus also infected systemically the resistant C. annuum cv. Rubia R. The nucleotide sequence of part of the CP gene of this potyvirus shared 96-98% identity with that of other PepYMV isolates. The partial nucleotide sequence of the 3' NTR showed 94-96% identity with that of PepYMV. These data indicate that this potyvirus is a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Potyvirus; RT-PCR; ELISA; Resistance gene.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200017
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Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry Genet. Mol. Biol.
Mendes-da-Glória,Fernanda Januzzi; Mourão Filho,Francisco de Assis Alves; Camargo,Luis Eduardo Aranha; Mendes,Beatriz Madalena Januzzi.
Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.
Tipo: Info:eu-repo/semantics/article
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300026
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Capacidade de combinação e heterose para resistência a Puccinia polysora Underw. em milho Scientia Agricola
Silva,Herberte Pereira da; Barbosa,Mauricio Pires Machado; Nass,Luciano Lourenço; Camargo,Luis Eduardo Aranha.
A resistência genética é o método mais eficiente de controle das doenças foliares da cultura do milho. Para avaliar a capacidade específica e geral de combinação (CEC e CGC, respectivamente) e heterose para resistência a Puccinia polysora, nove linhagens e seus 36 híbridos F1 foram utilizados em experimentos conduzidos em três ambientes. A severidade da doença foi avaliada na planta inteira (PI) e na folha posicionada no ponto de inserção da espiga principal (AFA). O delineamento experimental utilizado foi em blocos casualizados com três repetições e a parcela experimental foi representada por uma fileira de 5 m de comprimento. A análise da variância para as reações a ferrugem polissora foram feitas usando a análise II do método de Gardner &...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Análise dialélica; Ferrugem polissora; Resistência a doença; Melhoramento vegetal.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000400020
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Chromosomal locations of the maize (Zea mays L.) HtP and rt genes that confer resistance to Exserohilum turcicum Genet. Mol. Biol.
Ogliari,Juliana Bernardi; Guimarães,Marco Antônio; Camargo,Luis Eduardo Aranha.
We used 125 microsatellite markers to genotype the maize (Zea mays L.) near isogenic lines (NIL) L30HtPHtPRtRt and L30htphtpRtRt and the L40htphtprtrt line which contrast regarding the presence of the recently described dominant HtP and the recessive rt genes that confer resistance to Exserohilum turcicum. Five microsatellite markers revealed polymorphisms between the NIL and were considered candidate linked markers for the HtP resistance gene. Linkage was confirmed by bulked segregant sample (BSS) analysis of 32 susceptible and 34 resistant plants from a BC1F1 population derived from the cross (L30HtPHtPRtRt x L40htphtprtrt) x L40htphtprtrt. The bnlg198 and dupssr25 markers, both located on maize chromosome 2L (bin 2.08), were polymorphic between bulks....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bulked segregant sample analysis; Microsatellite markers; Maize; Near-isogenic lines; Northern leaf blight.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400021
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Controle genético da resistência à mancha-de-Phaeosphaeria em milho Ciência Rural
Lopes,Maria Teresa Gomes; Lopes,Ricardo; Brunelli,Kátia Regiane; Silva,Herberte Pereira da; Matiello,Rodrigo Rodrigues; Camargo,Luis Eduardo Aranha.
A mancha-de-Phaeosphaeria, de ampla ocorrência no Brasil, tem causado expressiva redução na produtividade da cultura do milho no país. Por isso, o desenvolvimento de híbridos resistentes à doença é um dos principais objetivos dos programas de melhoramento genético da cultura. Informações sobre o controle genético da resistência à doença são necessárias para que os programas de melhoramento sejam eficientes. O objetivo deste estudo foi determinar o controle genético da resistência à mancha-de-Phaeosphaeria em milho a partir da avaliação das médias de gerações oriundas de dois cruzamentos entre linhagens resistentes (DAS95 ou DAS72) com uma suscetível (DAS21), sob condições de infecção natural da doença. Os ensaios foram conduzidos em Indianópolis (MG), em...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaeosphaeria maydis; Resistência genética; Análise de média de gerações; Melhoramento genético.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000300001
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Genetic analysis of soybean resistance to Fusarium solani f.sp. glycines Genet. Mol. Biol.
Fronza,Vanoli; Vello,Natal Antonio; Camargo,Luis Eduardo Aranha.
In order to study the genetic control of soybean resistance to sudden death syndrome (SDS), a 5 x 5 diallel with the F2 generation, without the reciprocals, was carried out in a greenhouse. The following parents were used: Forrest, MG/BR-46 (Conquista), IAC-4, FT-Cristalina, and FT-Estrela. The first two cultivars are more resistant to SDS than IAC-4, which is considered to be moderately resistant to SDS, and the last two cultivars are highly susceptible. The fungus was inoculated with three colonized sorghum grains placed at the bottom of the holes with two soybean seeds. Single plants were evaluated between 14 and 37 days after emergency based on foliar severity symptoms (1-5) of SDS. The disease incidence and a disease index were also calculated for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Resistance; Sudden death syndrome-SDS; Diallel crosses; Jinks-Hayman; Molecular markers SSR.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300016
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Identificarion of contaminant bacteria in cachaça yeast by 16s rDNA gene sequencing Scientia Agricola
Carvalho-Netto,Osmar Vaz de; Rosa,Daniel Dias; Camargo,Luis Eduardo Aranha.
Cachaça is a typical Brazilian liquor produced from the distillation of fermented sugarcane juice mainly by Saccharomyces cerevisiae. Most of the domestic production is artisanal, and producers usually are not concerned regarding microbiological control of the fermentation. This study aimed to characterize the contaminant bacterial community of the yeast used in the production of cachaça in an artisanal still. Four samples were collected, of which one (NA) was used for comparison purposes and was collected one year earlier. The remaining samples were collected at three different periods: at the end of the first day of fermentation (NP), after fifteen days (NS), and thirty days after the same yeast was used (NT). Five hundred and eighty-seven sequences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aguardente; Alcoholic fermentation; Genetic characterization; Pé-de-cuba.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500010
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Interaction between resistance to Septoria tritici and phenological stages in wheat Scientia Agricola
Gieco,Jorge Omar; Dubcovsky,Jorge; Camargo,Luis Eduardo Aranha.
Studies dedicated to understanding the behavior of pathogens in relation to their hosts are the starting point for any breeding program aimed to develop resistant cultivars. The objective of this study was to analyze the resistance to Septoria tritici Rob in 77 F11 progenies of wheat derived from the three-way cross Tadinia <FONT FACE=Symbol>´</FONT> (Yecora rojo <FONT FACE=Symbol>´</FONT> UC554) in the phenological stages of seedling, tillering, and flag leaf/flowering. Three field experiments were conducted where plants were artificially inoculated at one of the above mentioned phenological stages by spraying with a suspension of conidia. A randomized block design with four replications was used in each experiment. Disease...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycosphaerella graminicola; Triticum aestivum; Leaf blotch; Disease resistance.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400011
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Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans Genet. Mol. Biol.
Bassi,Denis; Briñez,Boris; Rosa,Juliana Santa; Oblessuc,Paula Rodrigues; Almeida,Caléo Panhoca de; Nucci,Stella Maris; Silva,Larissa Chariel Domingos da; Chiorato,Alisson Fernando; Vianello,Rosana Pereira; Camargo,Luis Eduardo Aranha; Blair,Matthew Wohlgemuth; Benchimol-Reis,Luciana Lasry.
Abstract Angular leaf spot (ALS) and powdery mildew (PWM) are two important fungi diseases causing significant yield losses in common beans. In this study, a new genetic linkage map was constructed using single sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs), in a segregating population derived from the AND 277 x SEA 5 cross, with 105 recombinant inbred lines. Phenotypic evaluations were performed in the greenhouse to identify quantitative trait loci (QTLs) associated with resistance by means of the composite interval mapping analysis. Four QTLs were identified for ALS resistance. The QTL ALS11AS, linked on the SNP BAR 5054, mapped on chromosome Pv11, showed the greatest effect (R2 = 26.5%) on ALS phenotypic variance. For PWM resistance,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudocercospora griseola; Erysiphe polygoni; Quantitative inheritance; SSRs; SNPs.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100109
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New resistance genes in the Zea mays: exserohilum turcicum pathosystem Genet. Mol. Biol.
Ogliari,Juliana Bernardi; Guimarães,Marco Antônio; Geraldi,Isaías Olívio; Camargo,Luis Eduardo Aranha.
The use of monogenic race-specific resistance is widespread for the control of maize (Zea mays L.) helminthosporiosis caused by Exserohilum turcicum. Inoculation of 18 Brazilian isolates of E. turcicum onto elite maize lines containing previously identified resistance genes and onto differential near-isogenic lines allowed the identification of new qualitative resistance genes. The inoculation of one selected isolate on differential near-isogenic lines, F1 generations and a BC1F1 population from the referred elite lines enabled the characterization of the resistance spectrum of three new genes, one dominant (HtP), one recessive (rt) and a third with non-identified genetic action. Three physiological races of the pathogen were also identified including two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Northern leaf blight; Genetic inheritance; Resistance genes; Races; Maize.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300017
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Resistência de variedades comerciais de cana-de-açúcar ao agente causal do raquitismo-da-soqueira Ciência Rural
Gagliardi,Paulo Roberto; Camargo,Luis Eduardo Aranha.
O raquitismo-da-soqueira (RSD), causado pela bactéria Leifsonia xyli subsp. xyli, é uma das mais importantes doenças da cana-de-açúcar nas regiões produtoras do mundo. O presente trabalho teve como objetivo avaliar o grau de resistência de 10 variedades comerciais RB de cana-de-açúcar mais a variedade CB 49-260, a qual foi incluída como padrão suscetível. O trabalho visou avaliar os danos amostrados em campo pelo peso das parcelas em função da relação entre a produtividade de parcelas inoculadas e não inoculadas artificialmente com a bactéria. Os resultados mostraram que as variedades RB 72 454, RB 83 5486, RB 86 7515, RB 92 5211, RB 92 5268, RB 92 5345, RB 92 8064 e a variedade padrão CB 49-260 comportaram-se como variedades suscetíveis. A variedade RB 85...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Raquitismo-da-soqueira; Leifsonia xyli subsp. xyli; Inoculação.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000400039
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Sensibilidade dos microssatélites para determinar a pureza varietal em sementes de milho Rev. bras. sementes
Ramos,Nilza Patrícia; Brunelli,Katia Regiane; Camargo,Luis Eduardo Aranha; Marcos Filho,Julio.
Em um sistema de produção de sementes, o limite de contaminação varietal em lotes de linhagens de milho é zero, de modo que a presença de apenas uma semente de genótipo estranho acarreta a reprovação do lote. Várias técnicas vêm sendo estudadas para determinar a pureza varietal, incluindo marcadores moleculares baseados em polimorfismo de DNA. Nessa pesquisa foi avaliada a sensibilidade da técnica de microssatélites para detectar a presença de sementes de outros genótipos em lotes de linhagens de milho. Utilizaram-se quatro linhagens (L1, L2, L3 e L4), onde as sementes da L2 eram contaminantes da L1 e, as da L4, contaminantes da L3. Para simulação de diferentes níveis de contaminação, 0, 1, 2, 5 e 10 sementes do genótipo estranho foram misturadas a "bulks"...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Análise de sementes; Marcadores moleculares; Linhagens.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100014
Registros recuperados: 17
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