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Registros recuperados: 5
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Exposure of marine mussels Mytilus spp. to polystyrene microplastics: Toxicity and influence on fluoranthene bioaccumulation ArchiMer
Paul-pont, Ika; Lacroix, Camille; Gonzalez Fernandez, Carmen; Hegaret, Helene; Lambert, Christophe; Le Goic, Nelly; Frere, Laura; Cassone, Anne-laure; Sussarellu, Rossana; Fabioux, Caroline; Guyomarch, Julien; Albentosa, Marina; Huvet, Arnaud; Soudant, Philippe.
The effects of polystyrene microbeads (micro-PS; mix of 2 and 6 μm; final concentration: 32 μg L−1) alone or in combination with fluoranthene (30 μg L−1) on marine mussels Mytilus spp. were investigated after 7 days of exposure and 7 days of depuration under controlled laboratory conditions. Overall, fluoranthene was mostly associated to algae Chaetoceros muelleri (partition coefficient Log Kp = 4.8) used as a food source for mussels during the experiment. When micro-PS were added in the system, a fraction of FLU transferred from the algae to the microbeads as suggested by the higher partition coefficient of micro-PS (Log Kp = 6.6), which confirmed a high affinity of fluoranthene for polystyrene microparticles. However, this did not lead to a modification...
Tipo: Text Palavras-chave: Microplastics; Fluoranthene; Mussel; Depuration; Oxidative system.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00344/45502/45848.pdf
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First description of French V. tubiashii strains pathogenic to mollusk: I. Characterization of isolates and detection during mortality events ArchiMer
Travers, Marie-agnes; Mersni Achour, Rachida; Haffner, Philippe; Tourbiez, Delphine; Cassone, Anne-laure; Morga, Benjamin; Doghri, Ibtissem; Garcia, Celine; Renault, Tristan; Fruitier-arnaudin, Ingrid; Saulnier, Denis.
Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was...
Tipo: Text Palavras-chave: Crassostrea gigas Pathogenicity Extracellular products Polyphasic approach; Real time PCR.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/30028/28743.pdf
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Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling ArchiMer
Becheler, Ronan; Cassone, Anne-laure; Noel, Philippe; Mouchel, Olivier; Morrison, Cheryl L.; Arnaud-haond, Sophie.
Sampling in the deep sea is a technical challenge, which has hindered the acquisition of robust datasets that are necessary to determine the fine-grained biological patterns and processes that may shape genetic diversity. Estimates of the extent of clonality in deep-sea species, despite the importance of clonality in shaping the local dynamics and evolutionary trajectories, have been largely obscured by such limitations. Cold-water coral reefs along European margins are formed mainly by two reef-building species, Lophelia pertusa and Madrepora oculata. Here we present a fine-grained analysis of the genotypic and genetic composition of reefs occurring in the Bay of Biscay, based on an innovative deep-sea sampling protocol. This strategy was designed to be...
Tipo: Text Palavras-chave: Cold-water coral; Lophelia pertusa; Madrepora oculata; Clonality; Fine-grained spatial genetic structure; Standardized sampling.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00300/41117/40291.pdf
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Microplastic bacterial communities in the Bay of Brest: Influence of polymer type and size ArchiMer
Frere, Laura; Maignien, Lois; Chalopin, Morgane; Huvet, Arnaud; Rinnert, Emmanuel; Morrison, Hilary; Kerninon, Sandrine; Cassone, Anne-laure; Lambert, Christophe; Reveillaud, Julie; Paul-pont, Ika.
Microplastics (<5 mm) exhibit intrinsic features such as density, hydrophobic surface, or high surface/volume ratio, that are known to promote microbial colonization and biofilm formation in marine ecosystems. Yet, a relatively low number of studies have investigated the nature of microplastic associated bacterial communities in coastal ecosystems and the potential factors influencing their composition and structure. Here, we characterized microplastics collected in the Bay of Brest by manual sorting followed by Raman spectroscopy and studied their associated bacterial assemblages using 16S amplicon high-throughput sequencing. Our methodology allowed discriminating polymer type (polyethylene, polypropylene and polystyrene) within small size ranges...
Tipo: Text Palavras-chave: Bacteria; Microplastics; Coastal ecosystem; Metabarcoding; Vibrios.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56082/57614.pdf
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Microplastics in seafood: Benchmark protocol for their extraction and characterization ArchiMer
Dehaut, Alexandre; Cassone, Anne-laure; Frere, Laura; Hermabessiere, Ludovic; Himber, Charlotte; Rinnert, Emmanuel; Riviere, Gilles; Lambert, Christophe; Soudant, Philippe; Huvet, Arnaud; Duflos, Guillaume; Paul-pont, Ika.
Pollution of the oceans by microplastics (<5 mm) represents a major environmental problem. To date, a limited number of studies have investigated the level of contamination of marine organisms collected in situ. For extraction and characterization of microplastics in biological samples, the crucial step is the identification of solvent(s) or chemical(s) that efficiently dissolve organic matter without degrading plastic polymers for their identification in a time and cost effective way. Most published papers, as well as OSPAR recommendations for the development of a common monitoring protocol for plastic particles in fish and shellfish at the European level, use protocols containing nitric acid to digest the biological tissues, despite reports of...
Tipo: Text Palavras-chave: Microplastics; Digestion; Method; Seafood products; Tissue; Plastic integrity.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00335/44582/44469.pdf
Registros recuperados: 5
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