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Registros recuperados: 15
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A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies Inra
Galan, M.; Guivier, E.; Caraux, G.; Charbonnel, N.; Cosson, J.F..
Background: High-throughput sequencing technologies offer new perspectives for biomedical, agronomical and evolutionary research. Promising progresses now concern the application of these technologies to large-scale studies of genetic variation. Such studies require the genotyping of high numbers of samples. This is theoretically possible using 454 pyrosequencing, which generates billions of base pairs of sequence data. However several challenges arise: first in the attribution of each read produced to its original sample, and second, in bioinformatic analyses to distinguish true from artifactual sequence variation. This pilot study proposes a new application for the 454 GS FLX platform, allowing the individual genotyping of thousands of samples in one...
Tipo: Journal Article Palavras-chave:  .
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010d836c75&uri=/notices/prodinra1/2010/12/
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Analysis of major histocompatibility complex class II gene in water voles using capillary electrophoresis-single stranded conformation polymorphism Inra
Bryja, J.; Galan, M.; Charbonnel, N.; Cosson, J.-F..
Analysis of a single strand conformation polymorphism (SSCP) using capillary electrophoresis (CE) is a novel method to study polymorphism of DNA sequences in large scale population studies. We optimized CE-SSCP analysis to study the major histocompatibility complex (MHC) class II alpha gene (DQA) polymorphism. Short-chain linear polyacrylamide (6%) as sieving matrix, TrisCl (pH 8.5) as buffer for sample dilution, and 27 ° C, 9 kV as electrophoresis parameters were suitable for sufficient resolution of all alleles. We found that almost 25% of clones contained a PCR (polymerase chain reaction) artefact and strict criteria have to be applied when using cloning and sequencing to analyse the allelic diversity of MHC genes
Tipo: Journal Article Palavras-chave: MAJOR HISTOCOMPATIBILITY COMPLEX; MEGABACE; POPULATION GENETICS; QUISCA; RT1.BA; CAPILLARY ELECTROPHORESIS SINGLE STRANDED CONFORMATION POLYMORPHISM.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200792a93ff8&uri=/notices/prodinra1/2007/08/
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Association between the DQA MHC class II gene and Pumala virus infection in Myodes glareolus, the bank vole Inra
Deter, J.; Bryja, J.; Chaval, Y.; Galan, M.; Henttonen, H.; Laakkonen, J.; Voutilainen, L.; Vapalahti, O.; Vaheri, A.; Ribas Salvador, A.; Morand, S.; Cosson, J.F.; Charbonnel, N..
Puumala virus (PUUV) is a hantavirus specifically harboured by the bank vole, Myodes (earlier Clethrionomys) glareolus. It causes a mild form of hemorrhagic fever with renal syndrome (HFRS) in humans, called Nephropathia epidemica (NE). The clinical severity of NE is variable among patients and depends on their major histocompatibility complex (MHC) genetic background. In this study we investigated the potential role of class II MHC gene polymorphism in the susceptibility/resistance to PUUV in the wild reservoir M. glareolus. We performed an association study between the exon 2 of the DQA gene and PUUVantibodies considering a natural population of bank voles. Because immune gene polymorphism is likely to be driven by multiple parasites in the wild, we also...
Tipo: Journal Article Palavras-chave: MYODES GLAREOLUS; HANKAVIRUS; FIEVRE HEMORRAGIQUE AVEC SYNDROME RENAL; FHSR; CAMPAGNOL MYODES (CLETHRIONOMYS) GLAREOLUS; HANTAVIRUS; SSCP; COINERTIA; HELMINTHS; MITES; COWPOX VIRUS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009b2b7ea8d&uri=/notices/prodinra1/2009/03/
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Associations between MHC genes and Puumala virus infection in Myodes glareolus are detected in wild populations, but not from experimental infection data Inra
Guivier, E.; Galan, M.; Malé, P.J.G.; Kallio, E.V.; Voutilainen, L.; Henttonen, H.; Olsson, G.E.; Lundkvist, A.; Tersago, K.; Augot, D.; Cosson, J.F.; Charbonnel, N..
We analysed the influence of MHC class II Dqa and Drb genes on Puumala virus (PUUV) infection in bank voles (Myodes glareolus). We considered voles sampled in five European localities or derived from a previous experiment that showed variable infection success of PUUV The genetic variation observed in the Dqa and Drb genes was assessed by using single-strand conformation polymorphism and pyrosequencing methods, respectively. Patterns were compared with those obtained from 13 microsatellites. We revealed significant genetic differentiation between PUUV-seronegative and -seropositive bank voles sampled in wild populations, at the Drb gene only The absence of genetic differentiation observed at neutral microsatellites confirmed the important role of selective...
Tipo: Journal Article Palavras-chave: CAMPAGNOLS ROUSSÂTRES (GLAREOLUS MYODES); DIFFERENCIATION GENETIQUE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201170369f35&uri=/notices/prodinra1/2011/06/
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Characterization and PCR multiplexing of polymorphic microsatellite loci in the whipworm Trichuris arvicolae, parasite of arvicoline rodents and their cross-species utilization in T. muris, parasite of murines Inra
Deter, J.; Galan, M.; Charbonnel, N.; Cosson, J.F..
Trichuris arvicolae and T. muris are gastro-intestinal nematodes of respectively arvicoline and murine rodents. We aim to investigate the ecology of these Trichuris species using population genetics. We sampled nematodes from rodents trapped in the East of France. After confirming the species identification of the nematodes using ITS1-5.8S-ITS2 ribosomal DNA sequences, we isolated and characterized twelve dinucleotide microsatellite loci in T. arvicolae. A multiplex panel was developed. Application to a set of 30 individuals allowed clear and easy characterization of allele sizes. The number of alleles ranged from 2 to 6 per locus with observed heterozygosities ranging from 0 to 0.93. A test on eleven T. muris revealed that eight loci among twelve...
Tipo: Journal Article Palavras-chave: MICROSATELLITE MICROSATELLITE BANK; MULTIPLEX PCR; PARASITIC NEMATODE; POPULATION GENETICS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20103758e5d0&uri=/notices/prodinra1/2010/12/
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Coevolutionary relationship between helminth diversity and MHC class II polymorphism in rodents Inra
Goüy de Bellocq, J.; Charbonnel, N.; Morand, S..
Parasite-mediated selection on major histocompatibility complex (MHC) genes has mainly been explored at the intraspecific level, although many molecular studies have revealed trans-species polymorphism. Interspecific patterns of MHC diversity might reveal factors responsible for the long-term evolution of MHC polymorphism. We hypothesize that host taxa harbouring high parasite diversity should exhibit high levels of MHC genetic diversity. We test this assumption using data on rodent species and their helminth parasites compiled from the literature. Controlling for similarity due to common descent, we present evidence indicating that high helminth species richness in rodent species is associated with increased MHC class II polymorphism. Our results are...
Tipo: Journal Article Palavras-chave: POLYMORPHISME TRANS-SPECIFIQUE; HELMINTHE COMPARATIVE METHOD; HELMINTHS; MAJOR HISTOCOMPATIBILITY COMPLEX DIVERSITY; TRANS-SPECIES POLYMORPHISM.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009a10df6e3&uri=/notices/prodinra1/2010/08/
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Concomitant influence of helminth infection and landscape on the distribution of Puumala hantavirus in its reservoir, Myodes glareolus Inra
Ribas Salvador, A.; Guivier, E.; Xuéreb, A.; Chaval, Y.; Cadet, P.; Poulle, M.L.; Sironen, T.; Voutilainen, L.; Henttonen, H.; Cosson, J.F.; Charbonnel, N..
Background: Puumala virus, the agent of nephropathia epidemica (NE), is the most prevalent hantavirus in Europe. The risk for human infection seems to be strongly correlated with the prevalence of Puumala virus (PUUV) in populations of its reservoir host species, the bank vole Myodes glareolus. In humans, the infection risks of major viral diseases are affected by the presence of helminth infections. We therefore proposed to analyse the influence of both helminth community and landscape on the prevalence of PUUV among bank vole populations in the Ardennes, a PUUV endemic area in France. Results: Among the 313 voles analysed, 37 had anti-PUUV antibodies. Twelve gastro-intestinal helminth species were recorded among all voles sampled. We showed that PUUV...
Tipo: Journal Article Palavras-chave: HELMINTHE; HANTAVIRUS; FIEVRE HEMORRAGIQUE A SYNDROME RENAL; VIRUS PUUMALA; RESERVOIR HOTE.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011b57592c8&uri=/notices/prodinra1/2011/06/
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Density-related changes in selection pattern for major histocompatibility complex genes in fluctuating populations of voles Inra
Bryja, J.; Charbonnel, N.; Berthier, K.; Galan, M.; Cosson, J.F..
Host-pathogen interactions are of particular interest in studies of the interplay between population dynamics and natural selection. The major histocompatibility complex (MHC) genes of demographically fluctuating species are highly suitable markers for such studies, because they are involved in initiating the immune response against pathogens and display a high level of adaptive genetic variation. We investigated whether two MHC class II genes (DQA1, DRB) were subjected to contemporary selection during increases in the density of fossorial water vole (Arvicola terrestris) populations, by comparing the neutral genetic structure of seven populations with that estimated from MHC genes. Tests for heterozygosity excess indicated that DQA1 was subject to intense...
Tipo: Journal Article Palavras-chave: BALANCING SELECTION; LOCAL ADAPTATION; MHC; MAJOR HISTOINCOMPATIBILITY COMPLEX; POPULATION CYCLES.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200986a4a82d&uri=/notices/prodinra1/2010/08/
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Hantavirus-induced immunity in rodent reservoirs and humans Inra
Schönrich, G.; Rang, A.; Lütteke, N.; Raftery, M.J.; Charbonnel, N.; Ulrich, R.G..
Hantaviruses are predominantly rodent-borne pathogens, although recently novel shrew-associated hantaviruses were found. Within natural reservoir hosts, hantairuses do not cause obvious pathogenetic effects; transmission to humans, however, can lead to hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome, depending on the virus species involved. This review is focussed on the recent knowledge on hantavirus-induced immune responses in rodent reservoirs and humans and their impact on susceptibility, transmission, and outcome of hantavirus infections. In addition, this review incorporates a discussion on the potential role of direct cell-virus interactions in the pathogenesis of hantavirus infections in humans. Finally, questions for...
Tipo: Journal Article Palavras-chave: MUSARAIGNE; HOTES RESERVOIR; FIEVRE HEMORRAGIQUE VIRALE; IMMUNOPATHOGENESE; TRANSMISSION VIRAL HEMORRHAGIC FEVER; HANTAVIRUS CARDIOPULMONARY SYNDROME; HANTAVIRUSES; RODENT RESERVOIR; TRANSMISSION; CELL-MEDIATED PATHOGENESIS; IMMUNOPATHOGENESIS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011b275060d&uri=/notices/prodinra1/2011/07/
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Multiple parasites mediate balancing selection at two MHC class II genes in the fossorial water vole: insights from multivariate analyses and population genetics Inra
Tollenaere, C.; Bryja, J.; Galan, M.; Cadet, P.; Deter, J.; Chaval, Y.; Berthier, K.; Ribas Salvador, A.; Voutilainen, L.; Laakkonens, J.; Cosson, J.F.; Charbonnel, N..
We investigated the factors mediating selection acting on two MHC class II genes (DQA and DRB) in water vole (Arvicola scherman) natural populations in the French Jura Mountains. Population genetics showed significant homogeneity in allelic frequencies at the DQA1 locus as opposed to neutral markers (nine microsatellites), indicating balancing selection acting on this gene. Moreover, almost exhaustive screening for parasites, including gastrointestinal helminths, brain coccidia and antibodies against viruses responsible for zoonoses, was carried out. We applied a co-inertia approach to the genetic and parasitological data sets to avoid statistical problems related to multiple testing. Two alleles, Arte-DRB-11 and Arte-DRB-15, displayed antagonistic...
Tipo: Journal Article Palavras-chave: IMMUNOGENETIQUE; CO-INERTIE; GENES MHC; ANALYSE MULTIVARIEE CO-INERTIA; DQA AND DRB MHC GENES; IMMUNOGENETICS; MULTIVARIATE ANALYSIS; PARASITE-MEDIATED BALANCING SELECTION.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20091657fa4b&uri=/notices/prodinra1/2011/06/
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Negative relationships between cellular immune response, Mhc class II heterozygosity and secondary sexual trait in the montane water vole Inra
Charbonnel, N.; Bryja, J.; Galan, M.; Deter, J.; Tollenaere, C.; Chaval, Y.; Morand, S.; Cosson, J.F..
Heterogeneities in immune responsiveness may affect key epidemiological parameters and the dynamics of pathogens. The roles of immunogenetics in these variations remain poorly explored. We analysed the influence of Major histocompatibility complex (Mhc) genes and epigamic traits on the response to phytohaemagglutinin in males from cyclic populations of the montane water vole (Arvicola scherman). Besides, we tested the relevance of lateral scent glands as honest signals of male quality. Our results did not corroborate neither the hypotheses of genome-wide heterozygosity-fitness correlation nor the Mhc heterozygote advantage. We found a negative relationship between Mhc hetetozygosity and response to phytohaemagglutinin, mediated by a specific Mhc homozygous...
Tipo: Journal Article Palavras-chave: CAMPAGNOL; IMMUNOECOLOGIE; IMMUNOGENETIQUE ABUNDANCE CYCLES; DQA AND DRB; IMMUNOCOMPETENCE HANDICAP; MHC CLASS II GENES; PARASITE-MEDIATED BALANCING SELECTION; SEXUAL SELECTION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101e8c77ca&uri=/notices/prodinra1/2010/12/
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Puumala and Tula hantaviruses in France Inra
Plyusnina, A.; Deter, J.; Charbonnel, N.; Cosson, J.F.; Plyusnin, A..
The first genome sequences of Tula (TULV) and Puumala (PUUV) hantaviruses undoubtedly originated from France were recovered from tissue samples of European common voles and bank voles captured in Jura region. Genetic analysis of S and M segments of French PUUV strain revealed its highest similarity to strains from neighboring Belgium and Germany and also from Slovakia. On phylogenetic trees, French PUUV strain was placed within the central European lineage formed by strains from these three countries. Both of our French TULV strains clustered together and formed a distinct, well-supported genetic lineage.
Tipo: Journal Article Palavras-chave:  RNA VIRUSES; HANTAVIRUSES; PUUMALA VIRUS; TULA VIRUS; PHYLOGENETIC ANALYSIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010da822da1&uri=/notices/prodinra1/2011/06/
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Stress and demographic decline: a potential effect mediated by impairment of reproduction and immune function in cyclic vole populations Inra
Charbonnel, N.; Chaval, Y.; Berthier, K.; Deter, J.; Morand, S.; Palme, R.; Cosson, J.F..
The stress response is initially adaptive, operating to maintain homeostasis. However, chronic long-term exposure to stressors may have detrimental effects. We proposed that chronic stress may be a major factor in demographic vole cycles, inducing decline in high-density populations. We monitored four populations of the fossorial water vole Arvicola scherman, which undergo pluriannual demographic cycles in the Jura Mountains (France). Sampling was conducted during the high densities and the decline. We measured fecal corticosterone metabolites (FCMs) to assess stress levels and injected phytohemagglutinin to estimate the cell-mediated immune response. We demonstrated that stress levels increase between the high densities and the decline in most of the vole...
Tipo: Journal Article Palavras-chave: DECLIN DEMOGRAPHIQUE; CORTICOSTERONE  ; RODENT; DEMOGRAPHIC DECLINE; STRESS; POPULATION DYNAMICS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009776ecbf8&uri=/notices/prodinra1/2009/03/
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The intestinal nematode Trichuris arvicolae affects the fecundity of its host, the common vole, Microtus arvalis Inra
Deter, J.; Cosson, J.F.; Chaval, Y.; Charbonnel, N.; Morand, S..
Parasites have detrimental effects on host fitness. Consequently, they play a major role for host population dynamics. In this study, we investigated experimentally the impact of the nematode Trichuris arvicolae on the reproduction of its host, the common vole Microtus arvalis. Wild common voles were trapped in east of France and reared in standardized conditions before being experimentally infected. Infection with Trichuris arvicolae did not affect host consumption of food or water. Parasitized females gave birth to slightly less pups (mean 3.36 ± 0.38) than unparasitized females (mean 3.60 ± 0.40). Controlling for natal litter size using analysis of covariance (ANCOVA), T. arvicolae infection had a significant effect on the individual mass at birth, with...
Tipo: Journal Article Palavras-chave: NEMATODE; TRICHURIS ARVICOLAE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009ef3fc4a4&uri=/notices/prodinra1/2010/08/
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Tnf-a expression and promoter sequences reflect the balance of tolerance/ resistance to Puumala hantavirus infection in European bank vole populations Inra
Guivier, E.; Galan, M.; Ribas Salvador; Xuéreb, A.; Chaval, Y.; Olsson, G.E.; Essbauer, S.; Henttonen, H.; Voutilainen, L.; Cosson, J.F.; Charbonnel, N..
The tumor necrosis factor-alpha (TNF-alpha) influences the ability to limit parasite infection but its over-production might result in inflammatory disorders. The level of Tnf-alpha gene expression could thus mediate a balance of tolerance/resistance to infections. This study focused on Puumala hantavirus (PUUV) infection in its rodent host, the bank vole (Myodes glareolus). In humans, PUUV is responsible of a mild form of hemorrhagic fever with renal syndrome, nephropathia epidemica (NE). The severity of NE is associated with an over-production of TNF-alpha. By contrast, PUUV infection in bank vole is chronic and asymptomatic. It is likely that different coevolutionary histories between PUUV and its hosts could lead to different balances of...
Tipo: Journal Article Palavras-chave: HANTA VIRUS; FACTEUR DE NECROSE TUMORALE ALPHA; NEPHRITE EPIDEMIQUE; TOLERANCE; RESISTANCE; IMMUNOPATHOLOGIE; INFECTION; CAMPAGNOL ROUSSATRE MYODES GLAREOLUS; PUUMALA HANTAVIRUS; TUMOR NECROSIS FACTOR-ALPHA; NEPHROPATHIA EPIDEMICA; TOLERANCE; RESISTANCE; IMMUNOPATHOLOGY; RESERVOIR.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011e1f0c398&uri=/notices/prodinra1/2011/06/
Registros recuperados: 15
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