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Microwave-assisted extraction of phycobiliproteins from Porphyridium purpureum ArchiMer
Juin, Camille; Cherouvrier, Jean-rene; Thiery, Valerie; Gagez, Anne-laure; Berard, Jean-baptiste; Joguet, Nicolas; Kaas, Raymond; Cadoret, Jean-paul; Picot, Laurent.
In the present study, microwave-assisted extraction was first employed to extract the phycobiliproteins of Porphyridium purpureum (Pp). Freeze-dried Pp cells were subjected to microwave-assisted extraction (MAE) to extract phycoerythin (PE), phycocyanin (PC), and allophycocyanin (APC). MAE combined reproducibility and high extraction yields and allowed a 180- to 1,080-fold reduction of the extraction time compared to a conventional soaking process. The maximal PE extraction yield was obtained after 10-s MAE at 40 A degrees C, and PE was thermally damaged at temperatures higher than 40 A degrees C. In contrast, a flash irradiation for 10 s at 100 A degrees C was the best process to efficiently extract PC and APC, as it combined a high temperature necessary...
Tipo: Text Palavras-chave: Allophycocyanin; MAE; Microalgae; Microwave; Phycobiliprotein; Phycocyanin; Phycoerythrin; Porphyridium; Thylakoid.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00253/36439/35169.pdf
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Study on the microalgal pigments extraction process: Performance of microwave assisted extraction ArchiMer
Pasquet, Virginie; Cherouvrier, Jean-rene; Farhat, Firas; Thiery, Valerie; Piot, Jean-marie; Berard, Jean-baptiste; Kaas, Raymond; Serive, Benoit; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
The performance of microwaves irradiation (MAE and VMAE) to extract pigments from two marine microalgae was compared to conventional processes (cold and hot soaking and ultrasound-assisted extraction). Pigments were quantified by RP-HPLC and extraction performance was assessed regarding rapidity, reproducibility and extraction yields. Scanning electron microscopy was used at all extraction steps to assess the impact of the process on microalgal cell integrity. Freeze-drying and pigments extraction preserved microalgae cell integrity (except sonication) and evoked agglutination in superposed cells layers. All processes performed on Dunaliella tertiolecta (chlorophyte) lead to rapid pigments extraction, and equivalent pigments extraction yields, the absence...
Tipo: Text Palavras-chave: Pigments; Microalgae; Microwave; Extraction; Chlorophyll; Fucoxanthin; Carotene; MAE; VMAE.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00029/14059/11533.pdf
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