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Registros recuperados: 29
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Adding new pieces to the Azadinium (Dinophyceae) diversity and biogeography puzzle: Non-toxigenic Azadinium zhuanum sp. nov. from China, toxigenic A. poporum from the Mediterranean, and a non-toxigenic A. dalianense from the French Atlantic ArchiMer
Luo, Zhaohe; Krock, Bernd; Mertens, Kenneth; Nezan, Elisabeth; Chomerat, Nicolas; Bilien, Gwenael; Tillmann, Urban; Gu, Haifeng.
The marine planktonic dinophyceaen genus Azadinium is a primary source of azaspiracids, but due to their small size its diversity may be underestimated and information on its biogeography is still limited. A new Azadinium species, A. zhuanum was obtained from the East China Sea and Yellow Sea of China by incubating surface sediments. Five strains were established by isolating single germinated cells and their morphology was examined with light microscopy and scanning electron microscopy. Azadinium zhuanum was characterized by a plate pattern of Po, cp, X, 4′, 2a, 6′′, 6C, 5S, 6′′′, 2′′′′, by a distinct ventral pore at the junction of Po, the first and fourth apical plates, and a conspicuous antapical spine. Moreover, Azadinium poporum was obtained for the...
Tipo: Text Palavras-chave: Azadinium; Azaspiracid; AZA-2; AZA-2 phosphate; Biogeography; China; French Atlantic; Mediterranean Sea.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00386/49765/51155.pdf
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Ailadinium reticulatum gen. et sp. nov. (Dinophyceae), a New Thecate, Marine, Sand-Dwelling Dinoflagellate from the Northern Red Sea ArchiMer
Saburova, Maria; Chomerat, Nicolas.
A new photosynthetic, sand-dwelling marine dinoflagellate, Ailadinium reticulatum gen. et sp. nov., is described from the Jordanian coast in the Gulf of Aqaba, northern Red Sea, based on detailed morphological and molecular data. A. reticulatum is a large (53–61 μm long and 38–48 μm wide), dorsoventrally compressed species, with the epitheca smaller than the hypotheca. The theca of this new species is thick and peculiarly ornamented with round to polygonal depressions forming a foveate-reticulate thecal surface structure. The Kofoidian thecal tabulation is APC (Po, cp), 4′, 2a, 6′′, 6c, 4s, 6′′′, 1p, 1′′′′ or alternatively it can be interpreted as APC, 4′, 2a, 6′′, 6c, 4s, 6′′′, 2′′′′. The plate pattern of A. reticulatum is noticeably different from...
Tipo: Text Palavras-chave: Benthic dinoflagellates; Dinophyceae; Gulf of Aqaba; Jordan; LSU rDNA; Molecular phylogeny; Morphology; Red Sea; SSU rDNA; Taxonomy.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00226/33678/38183.pdf
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An emended description and phylogeny of the little-known Prorocentrum sipadanense Mohammad-Noor, Daugbjerg & Moestrup (Prorocentrales, Dinophyceae) from the Indian Ocean, Oman ArchiMer
Saburova, Maria; Chomerat, Nicolas.
A small Prorocentrum species was found during a pilot taxonomic survey of marine benthic dinoflagellates in the northwestern Arabian Sea, Oman. Based on the study of cells from natural samples and laboratory cultures using light and scanning electron microscopy, this taxon is attributed to the little-known Prorocentrum sipadanense, recorded so far only from East Malaysia. The description of this species has been emended to include details of internal cell structure, composition of its periflagellar area and intraspecific variability in size, shape and pore pattern. Round to oval or ovoid cells of P. sipadanese are surrounded by a prominent marginal ridge and are 17.9–23.9 µm long and 15.0–19.8 µm wide, with a foveate to reticulate thecal plate surface and...
Tipo: Text Palavras-chave: Arabian Sea; Benthic dinoflagellates; Molecular phylogeny; Morphology; Oman; Prorocentrum sipadanense; Taxonomy.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00319/42990/42560.pdf
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Cabra reticulata sp. nov. (Dinophyceae), a new sand-dwelling dinoflagellate from the Atlantic Ocean ArchiMer
Chomerat, Nicolas; Nezan, Elisabeth.
A new sand-dwelling dinoflagellate species, Cabra reticulata sp. nov., is described from sandy habitats in the south of Brittany (northwestern France). This new species possesses the characteristics of the 'unusual' genus, Cabra Murray et Patterson. The thecal plate formula is Po Pt 3' 1a 4'' 'x' 3c ?s 5''' 1'''' (or alternatively interpreted as Po Pt 4' 0a 4'' 'x' 3c ?s 5''' 1''''). Because of the strong lateral compression, cells of C. reticulata are frequently seen laterally and have a polygonal shape, with three prominent antapical pointed flanges and a dorsal spine in the anterior part of the hypotheca. The cingulum is ascending and incomplete. The thecal surface is ornamented with strong reticulations forming polygonal areolae, which differs from C....
Tipo: Text Palavras-chave: Taxonomy; Sand dwelling; Roscoffia; Protists; Podolampaceae; Marine; Dinophyceae; Benthic.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6804.pdf
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Complex Toxin Profile of French Mediterranean Ostreopsis cf. ovata Strains, Seafood Accumulation and Ovatoxins Prepurification ArchiMer
Brissard, Charline; Herrenknecht, Christine; Sechet, Veronique; Herve, Fabienne; Pisapia, Francesco; Harcouet, Jocelyn; Lemee, Rodolphe; Chomerat, Nicolas; Hess, Philipp; Amzil, Zouher.
Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins...
Tipo: Text Palavras-chave: Ostreopsis cf. ovata; Palytoxin & ovatoxins; Culture; Seafood contamination; LC-MS/MS; Hemolysis assay; Mediterranean Sea.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31025/29428.pdf
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Cyanobactéries dans le Lac d’Hossegor en 2013 ArchiMer
Rumebe, Myriam; Auby, Isabelle; Chomerat, Nicolas; Amzil, Zouher; Costa, Magali; Arrue, François.
Cette note concerne une efflorescence de cyanobactéries du genre Planktothrix dans le Lac marin d'Hossegor, en janvier 2013. La provenance de ces microalgues a été identifiée et leur innocuité démontrée grâce aux résultats d'analyses des phycotoxines.
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27834/26040.pdf
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Description and phylogenetic position of Plagiolemma distortum sp. nov., a new raphid diatom (Bacillariophyceae) from French coastal waters ArchiMer
Nezan, Elisabeth; Bilien, Gwenael; Boulben, Sylviane; Mertens, Kenneth; Chomerat, Nicolas.
A new marine raphid diatom, Plagiolemma distortum Nézan, sp. nov. is described from plankton samples from the Atlantic Ocean, south Brittany (north-western France), where it was regularly observed, but never in high abundances. Cells have an unusual and complex shape under light microscopy (LM) depending on their position and orientation. The two valves are highly vaulted and narrow in width (transapical axis). Cells contain four complexly lobed plastids and a variable number of refractive granules without particular localization. In addition, capsules containing motile cells were observed. Ultrastructural details of the frustules were investigated using scanning electron microscopy (SEM). The girdle bands are simple. Each valve possesses a sigmoid raphe...
Tipo: Text Palavras-chave: Capsules; Habitat; Molecular phylogeny; Plastids; Raphid diatom; Video.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00441/55235/56797.pdf
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Dynamics of phytoplankton communities in eutrophying tropical shrimp ponds affected by vibriosis ArchiMer
Lemonnier, Hugues; Lantoine, Francois; Courties, Claude; Guillebault, Delphine; Nezan, Elisabeth; Chomerat, Nicolas; Escoubeyrou, Karine; Galinie, Christian; Blockmans, Bernard; Laugier, Thierry.
Tropical shrimp aquaculture systems in New Caledonia regularly face major crises resulting from outbreaks of Vibrio infections. Ponds are highly dynamic and challenging environments and display a wide range of trophic conditions. In farms affected by vibriosis, phytoplankton biomass and composition are highly variable. These conditions may promote the development of harmful algae increasing shrimp susceptibility to bacterial infections. Phytoplankton compartment before and during mortality outbreaks was monitored at a shrimp farm that has been regularly and highly impacted by these diseases. Combining information from flow cytometry, microscopy, pigment and phylogenetic analysis, the presence of Picocyanobacteria, Prasinophyceae and Diatomophyceae were...
Tipo: Text Palavras-chave: Tropical aquaculture pond; Litopenaeus stylirostris; Vibriosis; Eutrophication; Phytoplankton; Harmful algae.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00343/45411/44986.pdf
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Essai d'aptitude par comparaison Inter-Analystes pour l'évaluation des performances en identification et dénombrement du phytoplancton marin dans le cadre du REPHY. Résultats exercice 2016 ArchiMer
Neaud-masson, Nadine; Brun, Melanie; Chomerat, Nicolas.
La détermination des espèces phytoplanctoniques et l’estimation de leur abondance dans le cadre du Réseau d’observation et de surveillance du Phytoplancton et de l’Hydrologie, mis en œuvre par l’Ifremer (REPHY) s’appuie en 2016 sur 25 analystes, répartis dans 9 laboratoires sur 12 sites. Neuf d’entre eux ont été inscrits à titre individuel en 2016 pour participer à l'exercice d’inter-comparaison international (IPI)  organisé tous les ans par le Marine Institute Phytoplankton unit de Galway (Ireland). Les échantillons envoyés aux analystes concernés ont été utilisés pour évaluer aussi les compétences des analystes non-inscrits à l’IPI, mais réalisant également des analyses de phytoplancton dans ces mêmes laboratoires pour le REPHY. Au total, 23 analystes...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00388/49923/50497.pdf
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Essai d'aptitude par comparaison Inter-analystes pour l'évaluation des performances en identification et dénombrement du phytoplancton marin. Rapport d'évaluation des compétences des analystes du phytoplancton dans le cadre du réseau d'observation et de surveillance du phytoplancton et de l'hydrologie (REPHY) mis en oeuvre par l'Ifremer ArchiMer
Neaud-masson, Nadine; Brun, Melanie; Chomerat, Nicolas.
The determination of phytoplankton species and the estimation of their abundance in the context of the Phytoplankton and Hydrology Monitoring and Surveillance Network, implemented by Ifremer (REPHY), is based in 2017 on 21 analysts spread over 12 sites. Proficiency testing by inter-laboratory or inter-analyst comparison is used to evaluate the performance of laboratories or analysts on specific skills. An International Phytoplankton Inter-comparison (IPI) is organized annually by the Marine Institute Phytoplankton unit of Galway (Ireland), to evaluate marine phytoplankton identification and enumeration performance. The IPI is implementing its inter-comparison exercises through the Marine Institute Ireland in cooperation with the Intergovernmental...
Tipo: Text Palavras-chave: Phytoplancton; Comparaison Inter-Laboratoire; CIL; Comparaison Inter-Analyste; CIA; Essai d’aptitude; Phytoplankton; Inter-Laboratory Comparison; CIL; Inter-Analyst Comparison; CIA; Aptitude Test; International Phytoplankton Inter-comparison; IPI.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00436/54797/56255.pdf
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Evolutionary processes and cellular functions underlying divergence in Alexandrium minutum ArchiMer
Le Gac, Mickael; Metegnier, Gabriel; Chomerat, Nicolas; Malestroit, Pascale; Quere, Julien; Bouchez, Olivier; Siano, Raffaele; Destombe, Christophe; Guillou, Laure; Chapelle, Annie.
Understanding divergence in the highly dispersive and seemingly homogeneous pelagic environment for organisms living as free drifters in the water column remains a challenge. Here, we analyzed the transcriptome wide mRNA sequences, as well as the morphology of 18 strains of Alexandrium minutum, a dinoflagellate responsible for Harmful Algal Blooms worldwide, to investigate the functional bases of a divergence event. Analysis of the joint site frequency spectrum (JSFS) pointed toward an ancestral divergence in complete isolations followed by a secondary contact resulting in gene flow between the two diverging groups, but heterogeneous across sites. The sites displaying fixed SNPs were associated with a highly restricted gene flow and a strong...
Tipo: Text Palavras-chave: Dinoflagellates; Harmful algal blooms; Populations Genomics; Pseudocryptic species; Speciation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45857/45529.pdf
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First record of resting cysts of the benthic dinoflagellate Prorocentrum leve in a natural reservoir in Gujan-Mestras, Gironde, France ArchiMer
Mertens, Kenneth; Gu, Haifeng; Pospelova, Vera; Chomerat, Nicolas; Nezan, Elisabeth; Gurdebeke, Pieter; Bogus, Kara; Vrielinck, Henk; Rumebe, Myriam; Meteigner, Claire.
The resting cysts of the benthic dinoflagellate Prorocentrum leve from a natural reservoir in Gujan-Mestras (Gironde, France) were described in this study. The incubated urn-shaped cysts gave rise to cells of Prorocentrum leve. Morphological observations through light microscopy and scanning electron microscopy, particularly of the periflagellar platelets, combined with large subunit ribosomal DNA sequences obtained through single-cell analysis confirm their affinity to the species Prorocentrum leve. The cysts are characterized by a specific shape and the presence of an anterior plug. This is the first conclusive evidence for fossilizable resting stages within the Prorocentrales, one of the major orders within the Dinophyceae. Palynological treatments show...
Tipo: Text Palavras-chave: Cellulosic; Micro-FTIR; Palynology; Prorocentrales; Resting stage.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00398/50971/51728.pdf
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First report of goniodomin A production by the dinoflagellate Alexandrium pseudogonyaulax developing in southern Mediterranean (Bizerte Lagoon, Tunisia) ArchiMer
Triki, Habiba Zmerli; Laabir, Mohamed; Moeller, Peter; Chomerat, Nicolas; Daly-yahia, Ons Kefi.
The dinoflagellate Alexandrium pseudogonyaulax is widely distributed around the world including the Mediterranean waters. The objectives of this study were to determine the morphology and phylogenic affiliation of A. pseudogonyaulax strain isolated from Bizerte Lagoon (Mediterranean waters, Tunisia) and investigate its toxicity. Molecular analyses confirmed the morphological identification of the isolated strain (APBZ12) as A. pseudogonyaulax. Moreover, it showed that it is 100% identical with strains of this species found in New Zealand, Japan, China and North Sea (Norway and Denmark) suggesting that this species is cosmopolitan. Until now, no toxin studies have been conducted on fully characterized (morphologically and molecularly) A. pseudogonyaulax....
Tipo: Text Palavras-chave: Alexandrium pseudogonyaulax; Goniodomin A; Mediterranean sea; Morphology; Phylogeny.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00302/41356/40548.pdf
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Fragilidium duplocampanaeforme sp nov (Dinophyceae): A new phagotrophic dinoflagellate from the French Atlantic coast ArchiMer
Nezan, Elisabeth; Chomerat, Nicolas.
A new species of the genus Fragilidium, F. duplocampanaeforme sp. nov., is described from examinations by LM and SEM. This species has been recorded in summer on the French Atlantic coast, over a number of years. It was never abundant in the plankton and was very often associated with Fragilidium subglobosum, Pyrophacus horologium and also with toxigenic species of the genera Alexandrium and Dinophysis. Phagotrophy of F. duplocampanaeforme on Dinophysis prey is shown, and sexual reproduction is suggested by the observation of gamete-like small forms. The size and the peculiar shape of its cells do not correspond to any known taxon, but the plate arrangement fits the genus Fragilidium. The plate formula is Po, Pc, 4', 8 '', 10c, 6s?, 7"', 2 '''', lp. A...
Tipo: Text Palavras-chave: Taxonomy; Phagotrophy; Fragilidium duplocampanaeforme sp nov.; Fragilidium; Dinophyceae; Atlantic ocean.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6233.pdf
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Genetic diversity of the harmful family Kareniaceae (Gymnodiniales, Dinophyceae) in France, with the description of Karlodinium gentienii sp. nov.: A new potentially toxic dinoflagellate ArchiMer
Nezan, Elisabeth; Siano, Raffaele; Boulben, Sylviane; Six, Christophe; Bilien, Gwenael; Cheze, Karine; Duval, Audrey; Le Panse, Sophie; Quere, Julien; Chomerat, Nicolas.
The family Kareniaceae is mostly known in France for recurrent blooms of Karenia mikimotoi in the Atlantic, English Channel, and Mediterranean Sea and for the unusual green discoloration in the saltwater lagoon of Diana (Corsica) caused by Karlodinium corsicum in April 1994. In terms of diversity, this taxonomic group was long overlooked owing to the difficult identification of these small unarmored dinoflagellates. In this study, thanks to the molecular characterization performed on single cells from field samples and cultures, twelve taxonomic units were assigned to the known genera Karenia, Karlodinium and Takayama, whereas one could not be affiliated to any described genus. The molecular phylogeny inferred from the D1–D2 region of the LSU rDNA showed...
Tipo: Text Palavras-chave: Genetic diversity; Haemolytic; Kareniaceae; Pigments; Single cell; Taxonomy.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00225/33611/32310.pdf
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Madanidinium loirii gen. et sp nov. (Dinophyceae), a new marine benthic dinoflagellate from Martinique Island, Eastern Caribbean ArchiMer
Chomerat, Nicolas; Bilien, Gwenael.
A new benthic phototrophic dinoflagellate is described from sediments of a tropical marine cove at Martinique Island and its micromorphology is studied by means of light and electron microscopy. The cell contains small golden-brown chloroplasts and the oval nucleus is posterior. It is laterally compressed, almost circular in shape when viewed laterally. It consists of a small epitheca tilted toward the right lateral side and a larger hypotheca. In the left view, the cingulum is more anterior and the epitheca is reduced. The cingulum is displaced and left-handed. This organism is peculiar in having no apical pore and its thecal plate arrangement is 2 ' 1a 7 '' 5c 3s 5 ''' 1 ''''. The plates are smooth with small groups of pores scattered on their surface....
Tipo: Text Palavras-chave: Caribbean; Benthic; Dinophyceae; SEM; Phylogeny; Morphology; Taxonomy; Martinique; RDNA.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00195/30622/29458.pdf
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Micro-algues des claires ostréicoles de Charente Maritime : focus sur les producteurs potentiels de toxines DSP, PSP et ASP et toxines émergentes (palytoxines, pinnatoxine...) ArchiMer
Mertens, Kenneth; Chomerat, Nicolas; Nezan, Elisabeth; Bruneau, Audrey; Le Bec, Claude.
Les inspections vétérinaires de la Commission Européenne ayant signifié à la France son constat d'une absence de réserau de surveillance sur les claires ostréicoles, la DGAL a mandaté l'Ifremer afin de réaliser un premier diagnostic sur le phytoplancton. Cette commande de la DGAL place l'Ifremer LERBO comme prestataire de service et spécialiste des micro-algues. Les claires ostréicoles étant hors du domaine public maritime, une concertation avec le Comité Régional de la Conchyliculture de Poitou-Charentes, la DGAL et la DDTM 17 en juin 2018 a permis de proposer six claires, réparties entre l'île de Ré et l'île d'Oléron et pouvant être échantillonnées. L'objectif est de faire un état des lieux de la présence ou de l'absence de cellules de phytoplancton...
Tipo: Text Palavras-chave: Charentes; Claire; Phytoplancton; Micro-algue; Toxine.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00486/59731/62828.pdf
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Micro-algues toxiques et nuisibles de l’océan mondial. COI Manuels et Guides, 68 ArchiMer
Lassus, Patrick; Chomerat, Nicolas; Hess, Philipp; Nezan, Elisabeth.
This book updates available international databases on toxic and harmful species of phytoplankton and micro-phytobenthos. It lists regional occurrences at world-wide scale: localities, cell densities, toxicity levels in contaminated animals, human and animal poisoning, isolation of identified active molecules and toxins, documentary sources. Finally, this book addresses the geographic distributions of phycotoxin-producers from recent data. The objective is to analyze the evolution of toxic and harmful episodes over the past 30 years to identify trends, and to introduce a discussion on the reality of a time-dependent increase in the number of known species and toxins.
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Ano: 2016 URL: https://archimer.ifremer.fr/doc/00379/49054/49508.pdf
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Morphology and molecular phylogeny of prorocentrum consutum sp. nov. (dinophyceae), a new benthic dinoflagellate from south Brittany (northwestern France) ArchiMer
Chomerat, Nicolas; Sellos, Daniel Y.; Zentz, Frederic; Nezan, Elisabeth.
A new marine benthic Prorocentrum species from sandy habitats of South Brittany (northwestern France), P. consutum sp. nov., is described using LM and SEM and molecular phylogenetic analyses. Cells have a subcircular to broadly ovoid shape and are plainly flattened. They are 57-61 lm long and 52-55 lm wide. A central pyrenoid is present, and the kidney-shaped nucleus is positioned in the posterior region. In right valve view, the periflagellar area is deeply excavated, and the left valve forms a prominent apical ridge. The periflagellar area consists of nine platelets, and a small narrow collar is present around the flagellar pore. The ornamentation of this new species is very peculiar and is characterized by a ring of round areolae located at the...
Tipo: Text Palavras-chave: Taxonomy; SSU rDNA; SEM; Prorocentrum; Nuclear DNA; Molecular phylogeny; LSU rDNA; Dinoflagellates.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/2010/publication-7449.pdf
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Morpho-molecular diversity and phylogeny of Bysmatrum (Dinophyceae) from the South China Sea and France ArchiMer
Luo, Zhaohe; Lim, Zhen Fei; Mertens, Kenneth; Gurdebeke, Pieter; Bogus, Kara; Carbonell-moore, Maria Consuelo; Vrielinck, Henk; Leaw, Chui Pin; Lim, Po Teen; Chomerat, Nicolas; Li, Xintian; Gu, Haifeng.
The dinoflagellate genus Bysmatrum encompasses five epibenthic or tide-pool species and has been characterized by separated anterior intercalary plates. In the present study, we obtained six strains of Bysmatrum from the South China Sea and French Atlantic coast by isolating single cells/cysts from plankton and sediment samples. All strains were examined with light microscopy and scanning electron microscopy. Based on morphological observations, three strains were identified as Bysmatrum subsalsum, characterized by the elongated and rectangular first and a hexagonal second anterior intercalary plate. They differ from each other in the number of sulcal lists and the configuration of the first anterior intercalary plate. One strain was identified as...
Tipo: Text Palavras-chave: Benthic dinoflagellate; Biogeography; Bysmatrum granulosum; Bysmatrum gregarium; Bysmatrum subsalsum; Eyespot; Molecular phylogeny.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00450/56188/57872.pdf
Registros recuperados: 29
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