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Sensitivity of eastern oyster ( Crassostrea virginica) spermatozoa and oocytes to dispersed oil: Cellular responses and impacts on fertilization and embryogenesis ArchiMer
Vignier, J.; Volety, A. K.; Rolton, A.; Le Goic, N.; Chu, F. -l. E.; Robert, Rene; Soudant, P..
The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF. Following a 30-min exposure, the number of spermatozoa and their viability were...
Tipo: Text Palavras-chave: Deepwater Horizon oil spill; Oyster; Spermatozoa; Oocyte; Flow-cytometry.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00377/48851/51168.pdf
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