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Registros recuperados: 16
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Adaptation de la méthode peptide-spot à la recherche de sites, de la protéine RNA2 du nodavirus, reconnus par différents sérums de loup (Dicentrarchus labrax). ArchiMer
Coeurdacier, Jean-luc.
Spot-Synthesis technique was applied to study protein frame or to develop antibodies with specific characteristics for use in diagnostic or research on human or mammals. In this work this technique was used to find sites on RNA2 protein of Nodavirus which are immunogenic for sea bass (Dicentrarchus labrax). It aimed to achieve synthetic peptide vaccine production against this virus. One hundred and nine overlapping peptides, of 15 amino acids frameshifted by three residues, representing the complete RNA2 protein sequence of Sb1 and Sb2 nodavirus were synthesized on a cellulose membrane by the Spot-Technique. The set of membrane bound peptides was probed by incubation with sea-bass or rabbit serum. Anti sea bass or anti rabbit alkaline phosphatase...
Tipo: Text Palavras-chave: Peptides synthétiques; Vaccin; Peptid-spot; Séquence immunogène; Dicentrarchus labrax; Synthetic peptides; Vaccine; Immunogenicity; Dicentrarchus labrax...
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00157/26810/24919.pdf
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Compte-rendu de mission en Tunisie (13-17 Juin 2005). Atelier de travail sur les nouvelles perspectives de la recherche scientifique en aquaculture ArchiMer
Thouard, Emmanuel; Buchet, Vincent; Cahu, Chantal; Coeurdacier, Jean-luc; Elie, Pierre; Gerard, André; Hussenot, Jerome; Legendre, Marc; Prou, Jean; Robert, Rene.
Les missions réalisées fin 2004 et début 2005 ont permis de faire le point sur les coopérations potentielles entre l'INSTM et l'IFREMER. Un certain nombre de domaines de coopération et de sujets de collaboration ont été identifiés et se sont traduits (ou se traduiront) par des actions de coopération (missions, stages, formations) soutenues financièrement par le SCAC de l'Ambassade de France à Tunis. La coopération entre l'IFREMER et l'INSTM dans le domaine de l'aquaculture a été très active dans le cadre du programme« Aquaculture 2001 » (1997-2001). Depuis cette période les relations se sont limitées à la préparation du programme DORAD qui était la suite d' «Aquaculture 2001 » . En l'absence du financement FSP sollicité pour DORAD, les deux organismes ont...
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Ano: 2005 URL: http://archimer.ifremer.fr/doc/00305/41648/40857.pdf
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De la maladie au bien-être, 20 ans d’évolution de la perception de la pathologie chez le loup d’élevage (Dicentrarchus labrax) ArchiMer
Coeurdacier, Jean-luc.
By looking at the common thread between 4 programs* in which the IFREMER Palavas (34, Languedoc-Roussillon region in France) station took part, this report show the evolution over 20 years in the perception of the pathology of farmed fish, and of the sea bass in particular (Dicentrarchus labrax). During the 1990’s farming techniques became sufficiently reliable to enable to distinguish between problems of a zootechnical nature and those with a pathological origin. In anticipation of future illnesses, the necessary tools and monitoring were implemented in order to study the immune system of the sea bass. These methods arose from the recent creation of anti-immunoglobin monoclonal probes in the sea bass. These techniques, together with others, served to...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27866/26103.pdf
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Dossier récapitulatif du développement de l’élevage du Platax orbicularis en Polynésie Française ArchiMer
Coeurdacier, Jean-luc; Gasset, Eric.
Platax orbicularis est l’espèce sur laquelle est basé le développement de la pisciculture en Polynésie française. Ce choix a été guidé par le fort potentiel économique de l’espèce dû à sa notoriété sur le marché local du poisson lagonaire estimé à 100 tonnes/an et à la diminution de sa population sauvage. Depuis 2001, date du démarrage d’un programme de développement de la pisciculture lagonaire en Polynésie, les travaux de recherche et développement menés conjointement par le Service de la Pêche de Polynésie (SPE) et l’IFREMER se sont focalisés sur l’acquisition de connaissances et de savoir-faire relatifs à la maîtrise zootechnique et sanitaire de l’élevage de ce poisson. En 2011 ce programme a atteint la première étape de transfert vers le secteur privé...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00134/24490/22514.pdf
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Effect of chronic exposure to ammonia on alterations of proteins and immunoglobulins in sea bass (Dicentrarchus labrax) serum ArchiMer
Coeurdacier, Jean-luc; Dutto, Gilbert.
The relationship between serum protein, immunoglobulin concentrations and protein molecular weight profile (PMWP) alterations of sea bass (131.3 +/- 4.3 g) reared in sea water with sublethal concentrations of ammonia was studied over two periods totalling 116 days. During the exposure period (62 days) the first group (group 1) lived in sea water with 0.204 mg.L-1 unionized ammonia nitrogen equivalent to 12 % of the lethal concentration for 50 % of a population exposed for 96 h to ammonia (96-h LC50), whilst the second group (group 2) lived in sea water with 0.340 mg.L-1 UIA-N equivalent to 20 % of the 96-h LC50 of ammonia. Then, the two groups were left for a recovery period (54 days) in the same water as the control group (group 0). The determination of...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Fish culture; Proteins; Immunoglobulin; Sublethal toxicity; Ammonia; Dicentrarchus labrax; Poisson d'élevage; Protéine; Immunoglobuline; Toxicité sublétale; Ammoniaque.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-858.pdf
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Effects of rearing density on sea bass (Dicentrarchus labrax) biological performance, blood parameters and disease resistance in a flow through system ArchiMer
Roque D'Orbcastel, Emmanuelle; Lemarie, Gilles; Breuil, Gilles; Petochi, Tommaso; Marino, Giovanna; Triplet, Sebastien; Dutto, Gilbert; Fivelstad, Sveinung; Coeurdacier, Jean-luc; Blancheton, Jean-paul.
During 84 days, the effects of density on juvenile sea bass (Dicentrarchus labrax) (76 +/- 16 g) were studied in an experimental tank-based flow through system. Performance, stress response and resistance to virus infection were analysed under five stabilized rearing densities: 10, 20, 40, 70 and 100 kg m(-3). Water quality parameters (CO2, total ammonia nitrogen and O-2) were measured and maintained close to the recommended values for farmed sea bass by adjusting water renewal exchange. No significant differences were observed between density treatments, neither on stress response (cortisol) nor susceptibility to nodavirus. With regards to biological performances, the daily feed intake and specific growth rate were significantly lower in fish reared at...
Tipo: Text Palavras-chave: Rearing density; Flow through system; Stress; Water quality; Sea bass.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11287/7824.pdf
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Influence de la mise en culture de l'Ilot des Tannes sur la qualité des eaux du chenal de Brouage - Campagnes 1984-1985 ArchiMer
Coeurdacier, Jean-luc; Faury, Nicole; Ratiskol, Jacqueline.
Les agriculteurs envisagent ou ont commencé d'aménager certains marais doux à des fins de culture. Si ces projets se réalisent, 500ha seront aménagés à terme (2 à 5 ans) dans le marais de Hiers Brouage qui en totalise 10 500. Les aménagements comportent : un isolement des ilots, une mise à plat, un assainissement par drainage et pompage de l'eau avec rejet direct ou indirect dans le milieu marin. Les administrations conscientes des risques de conflit entre agriculteurs d'une part, ostréiculteurs et associations de protection de la nature d'autre part ont souhaité qu'un suivi soit effectué sur l'Ilot des Tannes (115 ha) pris comme test. Le principe d'une "lagune" a été retenu afin d'arrêter les particules fines et provoquer une certaine stabulation des eaux...
Tipo: Text Palavras-chave: Qualité des eaux; Agriculture; Conchyliculture; Rejets agricoles; Chenal de Brouage; Charente-Maritime.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00087/19851/17501.pdf
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Is total serum protein a good indicator for welfare in reared sea bass (Dicentrarchus labrax)? ArchiMer
Coeurdacier, Jean-luc; Dutto, Gilbert; Gasset, Eric; Blancheton, Jean-paul.
Reared sea bass were submitted to six stressful situations: hyperoxia with or without hypercapnia, increased stocking density in an open flow or recirculating system, transfer to another tank and nodavirus injection. The potentially negative impact of these factors on the lives of sea bass was investigated by measuring 9 water parameters and 19 fish parameters including total serum protein (TSP). TSP has already been used to evaluate stress. The present study investigates data of previous and new experiments, concentrating on the potential use of TSP as a routine indicator to assess welfare in sea bass reared on fish farms. In the current experiment, oxygen and carbon dioxide were seen to affect levels of TSP, but alterations were too erratic to enable...
Tipo: Text Palavras-chave: Fish stress; Fish welfare; Protein; Serum; Fish transfer; Nodavirus; Sea bass.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15382/12740.pdf
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La maladie: fatalité ou échec ArchiMer
Coeurdacier, Jean-luc.
Cette présentation aborde la problématique de la santé des cheptels. La maladie provoque des pertes financières importantes à cause des mortalités mais aussi par les pertes en temps et en rentabilité. La maladie n’est pas due au hasard mais à la conjonction de la présence d’un agent responsable, de l’environnement et des techniques d ‘élevages. Il faut envisager le traitement des maladies non pour remplacer une phase défaillante de la méthode d’élevage mais comme un volet indispensable de la stratégie d’une entreprise.
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Ano: 1997 URL: http://archimer.ifremer.fr/doc/00160/27110/25293.pdf
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Preliminary study of immunogenicity and protection against nodavirus following injection of synthetic peptides from its capsid protein. ArchiMer
Coeurdacier, Jean-luc; Laporte, Florent; Pepin, Jean-francois.
Cette présentation orale aborde les aspects non traités dans la publication relative aux essais de vaccination contre la Nodavirose par peptides synthétiques (https://w3.ifremer.fr/archimer/doc/00000/10600/7038.pdf) dont évaluation par différentes méthodes de la présence ou des traces d’un contact avec le Nodavirus qu’il soit vivant (culture de cellule), sous forme de fraction protéique (ELISA antigène et PCR) ou qu’il ait laissé des traces sériques (séropositivité par ELISA anticorps) ou sous forme de vacuoles encéphaliques (immunohistochimie).
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Ano: 1999 URL: http://archimer.ifremer.fr/doc/00157/26828/24943.pdf
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Proteomic approach to investigate alterations, within physiological limits, in serum protein of sea bass (Dicentrarchus labrax) ArchiMer
Coeurdacier, Jean-luc.
In previous experiments reared fish were submitted to six stressful situations. The potential negative impact on their life was investigated using 9 water parameters and 19 fish parameters including global serum protein. Proteinemia allows to follow important stress situation but it cannot be used to detect small variations within physiological limits +like stocking density. For stocking density proteinemia is at the limit of its discriminating power. Studies of protein panel modification in sera by classic chromatographic method permit in the pass to go further but shown their limits too. Recently proteomic approaches were used on fish by using either 2D electrophoresis and MALDI-TOF or SELDI-TOF to investigate panel and variation in difference type of...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00133/24459/22474.pdf
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Rapport annuel d'activité 1980 Service des contrôles sanitaires et techniques de La Tremblade ArchiMer
Coeurdacier, Jean-luc.
Rapport annuel d'activité du Service des contrôles sanitaires et techniques de La Tremblade concernant l'année 1980
Tipo: Text Palavras-chave: Contrôle sanitaire; Conchyliculture; Bactériologie; Casier sanitaire; Importation; Exportation; Marennes Oléron.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/00070/18110/15658.pdf
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Rapport annuel d'activité 1981 Service des contrôles sanitaires et techniques de La Tremblade ArchiMer
Coeurdacier, Jean-luc.
Rapport annuel du Service des Contrôles Sanitaires et Techniques de La Tremblade concernant l'année 1981
Tipo: Text Palavras-chave: Conchyliculture; Contrôle sanitaire; Casier sanitaire; Bactériologie; Pollution; Assainissement; Importation; Exportation; Crassostrea gigas; Marennes Oléron.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/00070/18120/15668.pdf
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Rapport d'activité année 1979 de l'Inspection Principale de La Tremblade ArchiMer
Coeurdacier, Jean-luc.
Rapport sur l'activité du Laboratoire "Inspection Principale de La Tremblade" de l'année 1979
Tipo: Text Palavras-chave: Contrôle sanitaire; Gisements naturels; Reparcage; Epuration; Bactériologie; Crassostrea gigas; Marennes Oléron.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00070/18087/15635.pdf
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Recherche de moyens de dépistage et de prévention de la nodavirose du bar ArchiMer
Coeurdacier, Jean-luc; Breuil, Gilles; Pepin, Jean-francois; Mouchel, Olivier; Fauvel, Christian; Laporte, Florent; Thiery, Richard; Castric, J; Raymond, Jean-christophe; Baudin Laurencin, Félix.
Ce rapport clôture une collaboration, entreprise courant 97, entre l’Agence Nationale pour la Valorisation de la Recherche (ANVAR), l’Agence Française de Sécurité Sanitaire des Aliments(AFSSA), le Syndicat National des Aquaculteurs Marins (SFAM) et l’Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER). Deux lignées de nodavirus infectant le bar ont été mises en évidence avec une pathogénie variable selon l'âge et les facteurs extérieurs. Ce virus s’est montré d’une résistance exceptionnelle aux facteurs tant physiques que chimiques. Les diagnostics existants ont été adaptés ou réabordés sous un angle différent. Une étude épidémiologique a été réalisée chez les producteurs. La vaccination par peptides synthétiques a permis de sélectionner...
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Ano: 2002 URL: http://archimer.ifremer.fr/doc/00167/27874/26120.pdf
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The effect of density on sea bass (Dicentrarchus labrax) performance in a tank-based recirculating system ArchiMer
Sammouth, Sophie; Roque D'Orbcastel, Emmanuelle; Gasset, Eric; Lemarie, Gilles; Breuil, Gilles; Marino, Giovanna; Coeurdacier, Jean-luc; Fivelstad, Sveinung; Blancheton, Jean-paul.
Sea bass (Dicentrarchus tabrax) (135 +/- 4 g) were reared under tank-based recirculating aquaculture system for a 63-day period at four densities: 10, 40, 70, 100 kg m(-3). Fish performance, stress indicators (plasma cortisol, proteonemia plus other blood parameters-Na+, K+, glucose, pH, total CO2- and water quality were monitored. At the end of the 63-day period, resistance to infection was also studied by a nodavirus challenge. A 25-day test was performed on fish from two extreme densities (10 and 100 kg m(3)) and one intermediate density (40 kg m(3)). With regards to the different density treatments, there was no significant difference between the daily feed intake (DFI) and the specific growth rate (SGR) up to a density of 70 kg m(-3). No significant...
Tipo: Text Palavras-chave: Welfare; Performance; European sea bass; Recirculating system; Density.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6761.pdf
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