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Evaluation des rejets biologiques de juvéniles de platax (Platax orbicularis) : approche méthodologique ArchiMer
Colleter, Julie; Gasset, Eric; Buchet, Vincent; Coves, Denis.
Tipo: Text
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00020/13174/10232.pdf
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Gene-centromere mapping in meiotic gynogenetic European seabass ArchiMer
Oral, Munevver; Colleter, Julie; Bekaert, Michhttps://w3.ifremer.fr/archimer-admin/author.jsp#ael; Taggart, John B.; Palaiokostas, Christos; Mcandrew, Brendan J.; Vandeputte, Marc; Chatain, Beatrice; Kuhl, Heiner; Reinhardt, Richard; Peruzzi, Stefano; Penman, David J..
Background Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. Results From a single meiotic gynogenetic family consisting of 79 progeny, 42...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Meiotic gynogenesis; Isogenic lines; DdRAD seq; Genetic map; Gene-Centromere map; Aquaculture.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00388/49901/50462.pdf
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Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives ArchiMer
Colleter, Julie; Penman, David J.; Lallement, Stephane; Fauvel, Christian; Hanebrekke, Tanja; Osvik, Renate D.; Eilertsen, Hans C.; D'Cotta, Helena; Chatain, Beatrice; Peruzzi, Stefano.
Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (gamma), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities....
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00239/34996/33531.pdf
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Towards the development of clonal lines in the European sea bass (Dicentrarchus labrax L.): application of uniparental reproduction techniques with an insight into sea bass eggs ArchiMer
Colleter, Julie.
Clonal lines are a powerful scientific tool for improved genetic characterization of organisms used in research. Inbred fish lines can be produced in only two generations using uniparental reproduction techniques. Androgenesis, achieved with variable success in several freshwater species, has been attempted in the European sea bass (Dicentrarchus labrax L), a marine fish of commercial and scientific interest. The low yields of progenies inheriting only the paternal genome after UV-irradiation of eggs led to considerations on the occurrence of UV screening compounds in pelagic eggs. Mycosporine-like amino acids and gadusol were found in many marine and freshwater organisms, but their occurrence in fish eggs was not clearly related to a behavioral pattern...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Lignées clonales; Androgenèse; Gynogenèse; MAAs; Gadusol; Marqueurs microsatellites; Homozygotie; Hétérozygotie; Taux de recombinaison.; Dicentrarchus labrax; Clonal lines; Androgenesis; Gynogenesis; MAAs; Gadusol; Microsatellite markers; Homozygosity; Heterozygosity; Recombination rate..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00300/41138/40326.pdf
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