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Registros recuperados: 62
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45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor ArchiMer
Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf
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A biofilm-forming marine bacterium producing proteins as extracellular polymeric substances ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, François; Compere, Chantal; Combes, Didier.
EPS biochemical characterization and observation by epifluorescencemicroscopy. Development of a marine biofilmformation model onto a polystyrene microtiterplate using the “O’Tooleand Koltermethod”[1] adapted with the well known fluorescent DNA staining DAPI
Tipo: Text
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00089/19998/17661.pdf
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A Biosensor for Detection of DNA Sequences Based on a 50MHz QCM electronic oscillator circuit ArchiMer
Bustabad, E. A.; Garcia, G; Rodriguez-pardo, L.; Farina, J.; Perrot, H.; Gabrielli, C.; Bucur, B.; Lazerges, M.; Rose, D.; Compere, Chantal; Arnau, A..
This work deals with the development of a high sensibility DNA biosensor based on a 50MHz Quartz Crystal Microbalance (QCM) Oscillator Electronic Circuit. The designed QCM oscillator sensor is able to detect the presence of complementary DNAs in a solution that match the sequence on a given strand in function of the changes in the output frequency of the oscillator. The design is adapted so that the Barkhausen condition is satisfied even when the quartz is immerged in liquid media. Also a comparative study of the developed 50MHz biosensor in front of a QCM oscillator with smaller frequency is carried out, with object of checking if the sensibility of the oscillator increases, allowing to detect smaller concentrations of the complementary DNA.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00021/13198/10574.pdf
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A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D.
Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test. Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this...
Tipo: Text Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf
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Adhésion et substances polymériques extracellulaires d’une nouvelle espèce bactérienne marine ArchiMer
Leroy, Celine; Compere, Chantal; Delbarre Ladrat, Christine; Raguenes, Gerard; Ghillebaert, François; Combes, Didier.
Une nouvelle bactérie marine du genre Pseudoalteromonas sp., D41, formant rapidement des biofilms sur tout type de matériau (verre, acier inoxydable, Téflon) a été isolée en Baie de Brest. L’objectif de ce présent travail est de caractériser et décrire cette bactérie capable de produire des exopolymères constitués en majorité de protéines. Le rôle des protéines sécrétées, dans les mécanismes d’adhésion, est discuté.
Tipo: Text
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00088/19950/17617.pdf
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Antibiofilm Activity of the Marine Bacterium Pseudoalteromonas sp Strain 3J6 ArchiMer
Dheilly, Alexandra; Soum-soutera, Emmanuelle; Klein, Geraldine; Bazire, Alexis; Compere, Chantal; Haras, Dominique; Dufour, Aurelie.
Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and...
Tipo: Text
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00004/11530/11317.pdf
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Anti-rabbit immunoglobulin G detection in complex medium by PM-RAIRS and QCM Influence of the antibody immobilisation method ArchiMer
Briand, Elisabeth; Salmain, M; Compere, Chantal; Pradier, C.
Two antibody immobilisation procedures were compared to set up an immunosensor for goat anti-rabbit immunoglobulin (anti-rIgG), i.e. rIgG covalently bound or immobilised via affinity to protein A (PrA). In both cases, the first layer of protein was covalently bound to a mixed self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) and mercaptohexanol (C6OH) on a gold surface. The elaboration of the sensitive surfaces, as well as their selectivity and sensitivity were studied step by step by polarization modulation-reflection absorption infra-red spectroscopy (PM-RAIRS) and quartz crystal trticrobalance (QCM) with impedance measurement. QCM measurements showed that the viscoelastic properties of the antibody layer were markedly modified during the...
Tipo: Text Palavras-chave: RIgG immobilisation; PM RAIRS; QCM; Immunosensors.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3580.pdf
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Behaviour of stainless steel in natural seawater ArchiMer
Compere, Chantal; Le Bozec, Nathalie.
In this paper, investigations performed in natural and artificial seawater on stainless steels will be presented. They concerned studies on: biofilm formation, passive layers composition, electrochemical behaviour, localised corrosion and the evolution of these different parameters as a function of ageing time. According to literature surveys, the different aspects will be discussed. Some conclusions will be drawn concerning the actual knowledge on the behaviour of stainless steels in seawater.
Tipo: Text
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00089/19989/17653.pdf
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Bioactive Compounds Produced by Pseudoalteromonas Affect Marine Biofilm Formation ArchiMer
Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain.
Bacterial biofilms, frequently in association with algae, protozoa and fungi, are found on all submerged structures in the marine environment. Biofilms are responsible for a range of surface‐associated and diffusible signals, which may moderate the settling behaviour of cells, spores and larvae. Thus, marine microorganisms are a new source of bioactive compounds, which enhance or inhibit the settlement of organisms.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00089/20005/17667.pdf
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Biofilms en mileu marin ArchiMer
Compere, Chantal.
Introduction: Almost all submerged surfaces in the marine environment are subject to developing an organic film, with the exception of some living organisms, such as sponges, which do not undergo any great colonization, for no currently-known scientific reason. The presence of this biofilm is at the source of many problems in the oceanographic field and for marine business. This article introduces some drawbacks relating to the existence of this fouling and elaborates upon the main studies conducted at IFREMER concerning the most-used methods for combating it. However, the anti-fouling agents that are generally used for protecting facilities are toxic and can have disastrous consequences on the marine environment's fauna and flora. Moreover, the use of...
Tipo: Text Palavras-chave: Biofilm; Seawater.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-1698.pdf
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Biofouling protection for marine environmental sensors ArchiMer
Delauney, Laurent; Compere, Chantal; Lehaitre, Michel.
These days, many marine autonomous environment monitoring networks are set up in the world. These systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or underwater oceanographic structures. These systems commonly use various sensors to measure parameters such as dissolved oxygen, turbidity, conductivity, pH or fluorescence. Emphasis has to be put on the long term quality of measurements, yet sensors may face very short-term biofouling effects. Biofouling can disrupt the quality of the measurements, sometimes in less than a week. Many techniques to prevent biofouling on instrumentation are listed and studied by researchers and manufacturers. Very few of them...
Tipo: Text
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00008/11920/8626.pdf
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Biofouling protection for marine underwater observatories sensors ArchiMer
Delauney, Laurent; Compere, Chantal; Lehaitre, Michel.
These days, many marine autonomous environment monitoring networks are set up in the world. Such systems take advantage of existing superstructures such as offshore platforms, lightships, piers, breakwaters or are placed on specially designed buoys or deep sea fix stations. The major goal for these equipments is to provide in real time reliable measurements without costly frequent maintenance. These autonomous monitoring systems are affected by a well known phenomena in seawater condition which is biofouling. Consequently, such system without efficient biofouling protection is hopeless. This protection must be applied to the sensors and to the underwater acoustic communication equipments.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00107/21801/20176.pdf
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Biosensors for the detection and monitoring of Alexandrium minutum along the French coasts ArchiMer
Antoine, Elisabeth; Compere, Chantal; Cosnier, Serge; Crassous, Marie-pierre; Derbal, Hassina; Erard-le Denn, Evelyne; Gondran, Chantal; Guilminot, Elodie; Lehaitre, Michel; Mazeas, Florence; Moreau, Hervé; Perrot, Hubert; Pradier, Claire-marie; Salmain, Michèle.
The microalgae Alexandrium minutum produces neurotoxins responsible for paralytic shellfish poisoning, which upon accumulation in shellfish represent a human health risk. This species recurrently forms toxic blooms in the Northern part of the French Brittany coast. At present, algae identification relies on tedious microscope observations mostly performed by skilled taxonomists in laboratories. Newly emerging fields of molecular taxonomy, nanoscale technology and biotechnology offer opportunities for the development of in-situ biosensors for the detection and the monitoring of toxic algae.
Tipo: Text
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00089/19986/17650.pdf
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Building of an immunosensor: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency? ArchiMer
Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie.
Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved.
Tipo: Text Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf
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Chalcogenide Glass Optical Waveguides for Infrared Biosensing ArchiMer
Anne, Marie-laure; Keirsse, Julie; Nazabal, Virginie; Hyodo, Koji; Inoue, Satoru; Boussard-pledel, Catherine; Lhermite, Herve; Charrier, Joel; Yanakata, Kiyoyuki; Loreal, Olivier; Le Person, Jenny; Colas, Florent; Compere, Chantal; Bureau, Bruno.
Due to the remarkable properties of chalcogenide (Chg) glasses, Chg optical waveguides should play a significant role in the development of optical biosensors. This paper describes the fabrication and properties of chalcogenide fibres and planar waveguides. Using optical fibre transparent in the mid-infrared spectral range we have developed a biosensor that can collect information on whole metabolism alterations, rapidly and in situ. Thanks to this sensor it is possible to collect infrared spectra by remote spectroscopy, by simple contact with the sample. In this way, we tried to determine spectral modifications due, on the one hand, to cerebral metabolism alterations caused by a transient focal ischemia in the rat brain and, in the other hand, starvation...
Tipo: Text Palavras-chave: Planar waveguide; Fibre; Optical sensor; Chalcogenide.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6851.pdf
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Compte Rendu d’Activités 2012 de l’Unité de Recherche « Recherches et Développements Technologiques » ArchiMer
Compere, Chantal; Unité RDT.
Compte Rendu d’Activités 2012 de l’Unité de Recherche « Recherches et Développements Technologiques »
Tipo: Text
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00171/28240/26493.pdf
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De nouvelles voies d’inhibition des biofilms. Des micro-organismes producteurs de nouvelles molécules actives ArchiMer
Klein, Geraldine; Dufour, Alain; Compere, Chantal.
Lors d’une immersion en eau de mer, tous les matériaux et structures sont rapidement colonisés par des salissures d’origine biologique : des biofilms. La transformation des propriétés de surface dumatériau et la présence demicro- oumacro-organismes (bactéries, algues, balanes, larves) engendrent des risques accrus de corrosion localisée, la biodétérioration desmatériaux immergés, le blocage des fonctions mécaniques…Afin de contrôler le dépôt et le développement de ces biofilms, la méthode privilégiée est l’application de peintures anti-salissures. Cependant ces revêtements sont généralement toxiques pour l’environnement. C’est pourquoi, les travaux menés actuellement visent à isoler de nouvelles molécules, produites par les bactéries marines, dans le but...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00351/46213/45910.pdf
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Detection and molecular identification of Pseudo-nitzschia species in natural samples from the French coasts ArchiMer
Perez Blanco, Eva; Antoine, Elisabeth; Crassous, Marie-pierre; Compere, Chantal.
Domoic acid (DA) was first detected in France in mussel flesh in 1998, outbreak that was linked to the presence of Pseudo-nitzschia pseudodelicatissima. Ever since, the phytoplankton monitoring program (REPHY, Réseau de surveillance du Phytoplancton et des Phycotoxines) also monitors DA content in shellfish flesh. Amnesic shellfish poisoning (ASP) has been recurrently detected, and was over the sanitary threshold in the years 2000 and 2004 in Brittany and in 2002 in the Mediterranean coast (Amzil, 2001; Amzil pers. comm.). Toxicity events are usually dominated by several Pseudo-nitzschia species, although different strains might be or not be toxic (Bates et al., 1998). This is why an inventory of the species present in the French coasts and their molecular...
Tipo: Text
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00089/19988/17652.pdf
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Detection of polycyclic aromatic hydrocarbon (PAH) compounds in artificial sea-water using surface-enhanced Raman scattering (SERS) ArchiMer
Peron, Olivier; Rinnert, Emmanuel; Lehaitre, Michel; Crassous, Philippe; Compere, Chantal.
This paper reports an accurate synthesis of surface-enhanced Raman scattering (SERS) active substrates, based on gold colloidal monolayer, suitable for in situ environmental analysis. Quartz substrates were functionalized by silanization with (3-mercaptopropyl)trimethoxysilane (MPMS) or (3-aminopropyl)trimethoxysilane (APTMS) and they subsequently reacted with colloidal suspension of gold metal nanoparticles: respectively, the functional groups SH and NH2 bound gold nanoparticles. Gold nanoparticles were prepared by the chemical reduction of HAuCl4 using sodium tricitrate and immobilized onto silanized quartz substrates. Active substrate surface morphology was characterized with scanning electron microscopy (SEM) measurements and gold nanoparticles...
Tipo: Text Palavras-chave: Sensor; Sea water; PAH detection; SERS; Silanization; Gold nanoparticles.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6616.pdf
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Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit ArchiMer
Garcia-martinez, Gonzalo; Alonso Bustabad, Enrique; Perrot, Hubert; Gabrielli, Claude; Bucur, Bogdan; Lazerges, Mathieu; Rose, Daniel; Rodriguez-pardo, Loreto; Farina, Jose; Compere, Chantal; Arnau Vives, Antonio.
This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.
Tipo: Text Palavras-chave: DNA sequence detection; Quartz crystal microbalance; Electronic oscillator; Sensitivity; Frequency noise; Resolution.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00044/15487/12886.pdf
Registros recuperados: 62
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