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Registros recuperados: 9
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Cassava Pulp Enzymatic Hydrolysate as a Promising Feedstock for Ethanol Production BABT
Valeriano,Ixthá Hasselmann; Marques,Gabriel Lessa Lisboa; Freitas,Suely Pereira; Couri,Sonia; Penha,Edmar das Mercês; Gonçalves,Márcia Monteiro Machado.
ABSTRACT The aim of this study was to produce bioethanol from enzymatic hydrolysates of cassava pulp, a by-product of cassava flour manufacturing, using an alcohol-tolerant Saccharomyces cerevisiae strain. First, the best operational condition of the starch hydrolysis process was determined through a complete factorial design (24), with triplicates at the central point. The independent variables evaluated were: the concentrations of α-amylase (Termamyl 2X) and glucoamylase (AMG 300L) and both liquefaction and saccharification times. The most favorable hydrolysis condition in the assay was achieved using 0.517 mL AMG.g starch-1 and 0.270 mL Termamyl.g starch-1, with liquefaction and saccharification times of 1 and 2 h, respectively. The broth obtained at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Starch waste; Enzymatic hydrolysis; Alcoholic fermentation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100207
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Composição em ácidos graxos do óleo da polpa de açaí extraído com enzimas e com hexano Rev. Bras. Frutic.
Nascimento,Rhutynéia Joana Silva do; Couri,Sonia; Antoniassi,Rosemar; Freitas,Suely Pereira.
A palmeira Euterpe oleracea (Mart.), conhecida como açaizeiro, é nativa da Amazônia e tem-se destacado pelo potencial dos seus produtos, em especial pela importância econômica para a fruticultura regional. A polpa de açaí contém alto teor de óleo vegetal, cerca de 53% em base seca, e é uma fonte de ácidos graxos essenciais. Neste trabalho, a composição em ácidos graxos da fração lipídica da polpa de açaí foi determinada por cromatografia gasosa de alta resolução. O óleo foi obtido pelo processo enzimático ou por extração com n-hexano. A extração enzimática, em meio aquoso, tem sido indicada como uma alternativa para a obtenção de óleos vegetais derivados de polpas de frutas. Os resultados apresentaram diferenças não significativas na composição em ácidos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Óleo de açaí; Ácidos graxos; Tecnologia enzimática.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000200040
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Enzymatic technology to improve oil extraction from Caryocar brasiliense camb. (Pequi) Pulp. Rev. Bras. Frutic.
Mariano,Renata Gomes de Brito; Couri,Sonia; Freitas,Suely Pereira.
The present study aims to compare yield and quality of pequi pulp oil when applying two distinct processes: in the first, pulp drying in a tray dryer at 60ºC was combined with enzymatic treatment and pressing to oil extraction; in the second, a simple process was carried out by combining sun-drying pulp and pressing. In this study, raw pequi fruits were collected in Mato Grosso State, Brazil. The fruits were autoclaved at 121ºC and stored under refrigeration. An enzymatic extract with pectinase and CMCase activities was used for hydrolysis of pequi pulp, prior to oil extraction. The oil extractions were carried out by hydraulic pressing, with or without enzymatic incubation. The oil content in the pequi pulp (45% w/w) and the physicochemical characteristic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caryocar brasiliense; Sun drying; Hydraulic pressing; Oil quality index.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452009000300003
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Lipase Production by Aspergillus niger C by Submerged Fermentation BABT
Lima,Laisy Garcia Ribeiro; Gonçalves,Márcia Monteiro Machado; Couri,Sonia; Melo,Verônica Ferreira; Sant’Ana,Gizele Cardoso Fontes; Costa,Antônio Carlos Augusto da.
Abstract This study aimed to evaluate the effects of variables on the process of lipases production by Aspergillus niger C by submerged fermentation (SmF). The production assays were performed in shake flasks for 72 hours at 150 rpm and 32°C. First, a fractional factorial design 25-1 (FFD) was carried out to evaluate the effect of the following process variables: sucrose, ammonium sulphate, soybean oil, yeast extract concentration and pH. After the selection of the variables that significantly influenced the lipase production, a central composite rotational design 22 (CCRD) was used, aiming to find the most favorable operational conditions. The selected assay condition (15.0 g.L-1 sucrose, 4.0 g.L-1 ammonium sulphate, 4.0 g.L-1 soybean oil and 1.0 g.L-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipases; Submerged fermentation; Aspergillus nige; Experimental design..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100214
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Produção de celulases por Aspergillus niger por fermentação em estado sólido PAB
Rodríguez-Zúñiga,Ursula Fabiola; Farinas,Cristiane Sanchez; Bertucci Neto,Victor; Couri,Sonia; Crestana,Silvio.
O objetivo deste trabalho foi avaliar a produção enzimática de celulases pelo fungo filamentoso Aspergillus niger por fermentação em estado sólido de diferentes substratos. Foram avaliados os substratos sólidos bagaço de cana-de-açúcar, farelo de soja, farelo de trigo e misturas entre os substratos. Em substrato com 90% de bagaço e 10% de farelo de soja, avaliaram-se os efeitos do conteúdo de umidade (60, 70 e 80%, base úmida) e da suplementação com os meios indutores da atividade enzimática: sacarose, Mandels & Weber básico, Mandels & Weber modificado, com acréscimo de carboximetilcelulose, e Czapeck Dox. As maiores atividades de celulase total e endoglucanase, em farelo de trigo, foram obtidas após 72 horas de cultivo: 0,4 e 21,0 UI g-1,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bagaço de cana-de-açúcar; Energia renovável; Etanol celulósico; Hidrólise enzimática; Lignocelulose.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000800018
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Purification and characterisation of an extracellular phytase from Aspergillus niger 11T53A9 BJM
Greiner,Ralf; Silva,Lucineia Gomes da; Couri,Sonia.
An extracellular phytase from Aspergillus niger 11T53A9 was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. Purification was achieved by ammonium sulphate precipitation, ion chromataography and gel filtration. The purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kDa and exhibited maximal phytate-degrading activity at pH 5.0. Optimum temperature for the degradation of phytate was 55°C. The kinetic parameters for the hydrolysis of sodium phytate were determined to be K M = 54 µmol l-1 and k cat = 190 sec-1 at pH 5.0 and 37°C. The purified enzyme was rather specific for phytate dephosphorylation. It was shown that the phytase preferably...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Phytate-degrading enzyme; Phytate; Phytase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400010
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Selection of cellulolytic fungi isolated from diverse substrates BABT
Damaso,Mônica Caramez Triches; Terzi,Selma da Costa; Farias,Antonio Xavier; Oliveira,Ana Carolina Pereira de; Fraga,Marcelo Elias; Couri,Sonia.
The aim of the present work was to select filamentous fungi isolated from diverse substrates to obtain the strains with potential to produce the hydrolytic enzymes. From a total of 215 strains, seven strains from the soils, six from the plants and one from sugarcane bagasse were selected and identified as belonging to the Trichoderma, Penicillium and Aspergillus genera. The best hydrolytic activities obtained by semi-solid fermentation using these strains were approximately: 35; 1; 160; 170 and 120 U/gdm (CMCase, FPase, β-glucosidase, xylanase and polygalacturonase, respectively), demonstrating their potential to synthesize the enzymes compared with the results reported in the literature.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulases; Semi-solid fermentation; Filamentous fungi; Hydrolytic enzymes.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400005
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Selection of tannase-producing Aspergillus niger strains BJM
Pinto,Gustavo A.S.; Leite,Selma G.F.; Terzi,Selma C.; Couri,Sonia.
The aim of this work was to select strains of Aspergillus niger for tannase production. Growth of colonies in plates with tannic acid-containing medium indicated their ability to synthesize tannase. Tannase activity was also measured in solid-state fermentation. A. niger 11T25A5 was the best tannase producer (67.5 U.g-1/72 hours of fermentation).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tannase; Aspergillus niger; Screening solid-state; Fermentation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100006
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Utilization of agroindustrial residues for lipase production by solid-state fermentation BJM
Damaso,Mônica Caramez Triches; Passianoto,Moisés Augusto; Freitas,Sidinéa Cordeiro de; Freire,Denise Maria Guimarães; Lago,Regina Celi Araujo; Couri,Sonia.
The aim of this work was to produce lipases by solid-state fermentation (SSF) using, as substrate, agroindustrial residue supplemented with by-products from corn oil refining process or olive oil. For a group of ten fungi strains selected in the first steps, the lipase activity obtained by SSF varied from 7.7 to 58.6 U/g of dry substrate (gds). Among the evaluated strains, the Aspergillus niger mutant 11T53A14 was selected by presenting the best enzymatic production. For the fermentation tests, two substrates were also investigated: wheat bran and corn cob, both supplemented with olive oil. The best results were obtained with wheat bran. Additionally, three industrial by-products from corn oil refining (soapstock, stearin and fatty acids) were evaluated as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Solid state fermentation; Agroindustrial residues; Corn oil by-products.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400015
Registros recuperados: 9
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