SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

SOUZA, M. M. de; ZERLOTINI NETO, A.; GEISTLINGER, L.; TIZIOTO, P. C.; TAYLOR, J. F.; ROCHA, M. I. P.; DINIZ, W. J. S.; COUTINHO, L. L.; REGITANO, L. C. de A..

Transcription factors (TFs) are pivotal regulatory proteins that control gene expression in a contextdependent and tissue-specific manner. In contrast to human, where comprehensive curated TF collections exist, bovine TFs are only rudimentary recorded and characterized. In this article, we present a manually-curated compendium of 865 sequence-specific DNA-binding bovines TFs, which we analyzed for domain family distribution, evolutionary conservation, and tissue-specific expression. In addition, we provide a list of putative transcription cofactors derived from known interactions with the identified TFs. Since there is a general lack of knowledge concerning the regulation of gene expression in cattle, the curated list of TF should provide a basis for an...

A comprehensive manually-curated compendium of bovine transcription factors.

Palavras-chave: DNA bovino; Expressão gênica; Gene expression in cattle; Gene expression regulation.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102174

SOUZA, M. M. de; ZERLOTINI NETO, A.; GEISTLINGER, L.; TIZIOTO, P. C.; TAYLOR, J. F.; ROCHA, M. I. P.; DINIZ, W. J. S.; COUTINHO, L. L.; REGITANO, L. C. de A..

Allele- and parent-of-origin-specific effects on expression of the KCNJ11 gene: a candidate for meat tenderness in cattle.

Palavras-chave: DNA bovine; Gene expression in cattle; Gene expression regulation.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101203

SOUZA, M. M. de; NICIURA, S. C. M.; TIZIOTO, P. C.; IBELLI, A. M. G.; GASPARIN, G.; ROCHA, M. I. P.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; DINIZ, W. J. S.; OLIVEIRA, P. S. N. de; LIMA, A. O.; MUDADU, M. de A.; BARIONI JUNIOR, W.; COUTINHO, L. L.; REGITANO, L. C. de A..

bitstream/item/147026/1/Souza-et-al-2016.pdf

Allele specific expression analysis in bovine muscle tissue.

Palavras-chave: Nelore; Expressão alélica diferencial; Maciez da carne; Gado Nelore; Alleles.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1052240

SOUZA, M. M.; MOKRY, F. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A.; CESAR, A. S. M.; MORÉ, D.; MOURÃO, G.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; ZERLOTINI NETO, A.; REGITANO, L. C..

Imprinted genes have been target of many studies, mainly in human and mouse, and lately in bovines due to the interest of understanding the epigenetic mechanisms underlying important meat quality phenotypes and the possibility of applying it in animal breeding programs in the future. Genomic DNA from 146 steers was genotyped using the Illumina BovineHD BeadChip in order to identify heterozygous individuals with known allele origin.

Allele specific expression analysis in bovine muscle tissue.

Palavras-chave: Bioinformática; Expressão gênica; Genoma; Bioinformatics; Gene expression; Genome.

Ano: 2015

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035220

Detection of Co-expressed pathway modules associated with mineral concentration and meat quality in nelore cattle.

Palavras-chave: Bioinformática; Expressão gênica; Genoma; Bioinformatics; Genome; Gene expression.

Ano: 2015

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035029

DINIZ, W. J. S.; MAZZONI, G.; COUTINHO; BANERJEE, P.; GEISTLINGER, L.; CESAR, A. S. M.; BERTOLINI. F.; AFONSO, J.; OLIVEIRA, P. S. N.; TIZIOTO, P. C.; KADARMIDEEN, H. N.; REGITANO, L. C. de A..

Meat quality is a complex trait that is influenced by genetic and environmental factors, which includes mineral concentration. However, the association between mineral concentration and meat quality, and the specific molecular pathways underlying this association, are not well explored. We therefore analyzed gene expression as measured with RNA-seq in Longissimus thoracis muscle of 194 Nelore steers for association with three meat quality traits (intramuscular fat, meat pH, and tenderness) and the concentration of 13 minerals (Ca, Cr, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, and Zn). We identified seven sets of co-expressed genes (modules) associated with at least two traits, which indicates that common pathways influence these traits. From pathway analysis of...

FABP1 and SLC2A5 expression levels affect feed efficiency-related traits.

Palavras-chave: AMPK pathway; Co-expression analysis; RNA sequencing; Tenderness; Gordura Animal; Gado de Corte; Intramuscular fat; Beef quality; Beef cattle.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107117

DINIZ, W. J. S.; ROSA, K. O. da; TIZIOTO, P. C.; MOURÃO, G. B.; OLIVEIRA, P. S. N. de; SOUZA, M. M. de; REGITANO, L. C. de A..

Improving the efficiency of production to reduce the environmental footprints is pivotal to the sustainability of livestock systems. Despite the advances in cattle feed efficiency (FE) measurement and identification of potential mechanisms involved, much is still unclear regarding the genetic and biological basis of this trait. Nevertheless, lipid and carbohydrate metabolism have been outlined as important in determining efficient and inefficient animals. To address the role of genes partaking in these processes and previously involved with residual feed intake (RFI), we carried out a liver expression profile in Nelore steers (n = 83). Six target genes (FABP1, FADS2, PPP1R26, RGS2, SLC2A5, and UCP2) were measured by qPCR analysis. A general linear mixed...

Gene expression differences in Longissimus muscle of Nelore steers genetically divergent for residual feed intake.

Palavras-chave: Cattle feed efficiency; Relative growth rate; Average daily gain; Gene expression; Dry matter intake.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127498

TIZIOTO, P. L.; COUTINHO, L. L.; OLIVEIRA, P. S. N.; CESAR, A. S. M.; DINIZ, W. J. S.; LIMA, A. O.; ROCHA, M. I.; DECKER, J. E.; SCHNABEL, R. D.; MOURÃO, G. B.; TULLIO, R. R.; ZERLOTINI NETO, A.; TAYLOR, J. F.; REGITANO, L. C. de A..

Residual feed intake (RFI), a measure of feed efficiency (FE), is defined as the difference between the observed and the predictable feed intake considering size and growth of the animal. It is extremely important to beef production systems due to its impact on the allocation of land areas to alternative agricultural production, animal methane emissions, food demand and cost of production. Global differential gene expression analysis between high and low RFI groups (HRFI and LRFI: less and more efficient, respectively) revealed 73 differentially expressed (DE) annotated genes in Longissimus thoracis (LT) muscle of Nelore steers. These genes are involved in the overrepresented pathways Metabolism of Xenobiotics by Cytochrome P450 and Butanoate and...

Liver DNA methylation profile in Nelore cattle extremes for feed efficiency.

Palavras-chave: Genome-wide association studies; Quantitative trait locus; Gado de corte; Gene expression; Feed conversion; Beef cattle; Nellore.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1073482

ROCHA, M. I. P.; SOUZA, M. M. de; DINIZ, W. J. S.; LIMA, A. O. de; AFONSO, J.; TIZIOTO, P. C.; OLIVEIRA, P. N. de; REGITANO, L. C. de A.; KOLTES, J. E.; NICIURA, S. C. M..

The production cost of cattle is high, especially the fraction related to animal nutrition. The increase in feed efficiency may provide increased efficiency in beef production, minimizing feed expenditures, methane gas emission and nitrogen excretion.

Monoallelic expression of NNAT gene in Nelore steers skeletal muscle.

Palavras-chave: Feed efficiency; RRBS; Differential methylation; Beef production; Gado de Corte; Bovino; Gene expression regulation; Animal nutrition.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110134

Potential biomarkers for feed efficiency-related traits in nelore cattle identified by co-expression network and integrative genomics analyses.

SOUZA, M. M.; ZERLOTINI, A.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A. L.; MOKRY, F. B.; CESAR, A. S. M.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; REGITANO, L. C. A..

ABSTRACT: The NNAT gene plays a role on adipogenesis, brain development and insulin secretion. NNAT is described as paternal imprinted in bovine fetal tissue, but it is still underexplored in adult tissues. In order to improve the understanding of NNAT expression, muscle from Nellore was used to assess its allelic expression. Genomic DNA from 38 steers was genotyped for identification of heterozygous individuals with known allele origin. Total mRNA from muscle was extracted and sequenced by HiScanSQ, and afterwards the reads of each allele were counted for each animal. A total of 8 reciprocal heterozygous had more than 10 reads for the chosen SNP. All animals showed monoallelic expression, being 5 with paternal allele expressed, while 7 had the G allele...

Tipo: Artigo em anais de congresso (ALICE)

Palavras-chave: Neuronatin; Zebu; Genomic imprinting.

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1006155

LIMA, A. O. de; KOLTES, J. E.; DINIZ, W. J. S.; OLIVEIRA, P. S. N. de; CESAR, A. S. M.; TIZIOTO, P. L.; AFONSO, J.; SOUZA, M. de S.; PETRINI, J.; ROCHA, M. I. P.; CARDOSO, T. F.; ZERLOTINI NETO, A.; COUTINHO, L. L.; MOURÃO, G. B.; REGITANO, L. C. de A..

Feed efficiency helps to reduce environmental impacts from livestock production, improving beef cattle profitability. We identified potential biomarkers (hub genes) for feed efficiency, by applying co-expression analysis in Longissimus thoracis RNA-Seq data from 180 Nelore steers. Six co-expression modules were associated with six feed efficiency-related traits (p-value ≤ 0.05). Within these modules, 391 hub genes were enriched for pathways as protein synthesis, muscle growth, and immune response. Trait-associated transcription factors (TFs) ELF1, ELK3, ETS1, FLI1, and TCF4, were identified with binding sites in at least one hub gene. Gene expression of CCDC80, FBLN5, SERPINF1, and OGN was associated with multiple feed efficiency-related traits...

PRUNE2 gene has a potential effect on residual feed intake in Nellore cattle.

Palavras-chave: Longissimus thoracis; Biomarcadores; Expressão gênica; Feed efficiency; Hub genes; Systems biology; WGCNA; Bos Indicus; Gene expression.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124356

LIMA, A. O. D.; OLIVEIRA, P. S. N.; TIZIOTO, P. C.; SOMAVILLA, A. L.; DINIZ, W. J. S.; SILVA, J. V. D.; ANDRADE, S. C. S.; BOSCHIERO, C.; CESAR, A. S. M.; SOUZA, M. M.; ROCHA, M. I. P.; AFONSO, J.; BUSS, C. E.; MUDADU, M. de A.; MOURAO, G. B.; COUTINHO, L. L.; REGITANO, L. C. de A..

Residual feed intake (RFI) can increase the profitability of producers, reduce methane emission and land allocation to livestock production. However, this trait has late and costly measurements. Identifying gene expression changes combined with polymorphisms that affect residual feed intake variation is important for identify target regulatory polymorphisms that can be used in animal breeding programs. Diverse studies performed by our research group in a Nellore population, such as genome-wide association (GWA), association weight matrix (AWM) and RNA-seq analysis of liver tissue have been pointed Prune homolog 2 (Drosophila) (PRUNE2) as a potential candidate gene influencing feed efficiency. For this reason, we select this gene for a more detailed...