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Cytogenetics of Colletotrichum lindemuthianum (Glomerella cingulata f. sp. phaseoli) Trop. Plant Pathol.
Roca M.,M. Gabriela; Davide,Lisete C.; Mendes-Costa,Maria C..
Cytogenetic and morphological studies were conducted with Colletotrichum lindemuthianum (Glomerella cingulata f. sp. phaseoli), the pathogen responsible for anthracnose of common bean (Phaseolus vulgaris). In this species, there is some evidence of genomic variation but it is unknown whether the process occurs in a manner similar to other fungal genetic models. Six isolates from bean plants were used and sexual reproduction was observed in vitro. Meiosis and ascospore formation were investigated by cytogenetical approaches and light microscopy. To study the nucleus and chromosome numbers, a mixture of carmine and orcein propionic dyes was used. Nucleus divisions as well as ascospore maturation were asynchronous. Meiosis was observed in three isolates. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conidia cytogenetics; Chromosome polymorphism.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000400004
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Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae) Anais da ABC (AABC)
Pierre,Patrícia M.O.; Sousa,Saulo M.; Davide,Lisete C.; Machado,Marco A.; Viccini,Lyderson F..
Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cheotype; Cytogenetic; Cytotype; DNA content; RAPD; Lippia alba.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300018
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Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage Braz. J. Plant Physiol.
Faria,José M.R.; Davide,Lisete C.; Silva,Edvaldo A. A. da; Davide,Antonio C.; Pereira,Roselaine C.; van Lammeren,André A.M.; Hilhorst,Henk W.M..
The remarkably short storability of recalcitrant seeds has prevented their inclusion in programs of ex situ conservation. The causes of their desiccation sensitivity and rapid decline in viability during storage are not fully elucidated yet. In this study the highly recalcitrant, fully viable embryos of the tree species Inga vera subsp. affinis were stored under various conditions and analyzed physiologically and cytologically at intervals, in order to obtain more insights in their loss of viability during storage. Embryos stored fully hydrated at 5ºC fully lost viability in 18 d. Sealed storage of partially dehydrated embryos slightly prolonged their viability, with 95% germination being attained after 14 d of storage. However, after 30 d of storage,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abscisic acid; Polyethylene glycol; Recalcitrant seeds; Seed conservation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000400008
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Relationship between pollen morphology and chromosome numbers in Brazilian species of Lippia L. (Verbenaceae) Anais da ABC (AABC)
Sousa,Saulo M.; Pierre,Patrícia M.O.; Torres,Giovana A.; Davide,Lisete C.; Viccini,Lyderson F..
The pollen morphology and exine structure of 17 species of Lippia L. were investigated in this work using light and scanning electron microscopy. Among the species studied, 14 showed tricolporate pollen grains, two had tri- and tetracolporate pollen grains and a single species exhibited, only tetracolporate pollen. The amb ranged from triangular to square, and the shape varied from oblate-spheroidal to prolate-spheroidal. Three different types of exine ornamentation were observed: psilate, scabrate and perforate. In addition to morphological data, we found positive association between the chromosome numbers and size of pollen grains, and also between the length and width of the colpi. The results indicate that the characteristics of pollen grains in Lippia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome numbers; Lippia; Palynology; Palynomorphology; Pollen; Verbenaceae.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100147
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Template preparation for rapid PCR in Colletotrichum lindemuthianum BJM
Roca,M. Gabriela; Davide,Lisete C.; Wheals,Alan E..
Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthracnose; Hygromycin resistance; Internal transcribed spacer; Rapid screening; PCR methodology.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003
Registros recuperados: 5
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