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| Vigne, E.; Demangeat, G.; Komar, V.; Fuchs, M.. |
| The naturally occurring Grapevine fanleaf virus (GFLV) recombinant isolate A17b was recovered from its grapevine host by sap inoculation and serial passages onto Gomphrena globosa, a pseudo local lesion herbaceous host, and Chenopodium quinoa, a systemic herbaceous host, to characterize some of its biological properties. Sequence analysis of the CP gene, in which a recombinational event was previously detected, demonstrated the genetic stability of recombinant isolate A17b over a 5-year period in its natural host as well as in C. quinoa. Also, recombinant isolate A17b was graft transmissible, as shown by an in vitro heterologous approach, and transmitted by the nematode Xiphinema index as readily as nonrecombinant GFLV isolates. Furthermore, despite a... |
| Tipo: Journal Article |
Palavras-chave: VIRUS DU COURT NOUE DE LA VIGNE; ISOLAT; POUVOIR PATHOGENE; PLANTE HOTE; ISOLAT RECOMBINANT GRAPEVINE FANLEAF VIRUS; ISOLATE; CP GENE. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007d7973279&uri=/notices/prodinra1/2011/05/ |
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| Schellenberger, P.; Demangeat, G.; Lemaire, O.; Ritzenthaler, C.; Bergdoll, M.; Oliéric, V.; Sauter, C.; Lorber, B.. |
| The small icosahedral plant RNA nepovirus Grapevine fanleaf virus (GFLV) is specifically transmitted by anematode and causes major damage to vineyards worldwide. To elucidate the molecular mechanismsunderlying the recognition between the surface of its protein capsid and cellular components of its vector,host and viral proteins synthesized upon infection, the wild type GFLV strain F13 and a naturalmutant (GFLV-TD) carrying a Gly297Asp mutation were purified, characterized and crystallized. Subsequently,the geometry and volume of their crystals was optimized by establishing phase diagrams.GFLV-TD was twice as soluble as the parent virus in the crystallization solution and its crystals diffractedX-rays to a resolution of 2.7 Å. The diffraction limit of... |
| Tipo: Journal Article |
Palavras-chave: VIRUS; GRAPEVINE FANLEAF VIRUS; CRISTALLIZATION; PHASE DIAGRAM; AGAROSE; GEL; SOLUBILITY; VIRUS DU COURT-NOUE. |
| Ano: 2011 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201142bd24bc&uri=/notices/prodinra1/2011/05/ |
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