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Registros recuperados: 5
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Biological activities of a mixture of biosurfactant from Bacillus subtilis and alkaline lipase from Fusarium oxysporum BJM
Quadros,Cedenir Pereira de; Duarte,Marta Cristina Teixeira; Pastore,Gláucia Maria.
In this study, we investigate the antimicrobial effects of a mixture of a biosurfactant from Bacillus subtilis and an alkaline lipase from Fusarium oxysporum (AL/BS mix) on several types of microorganisms, as well as their abilities to remove Listeria innocua ATCC 33093 biofilm from stainless steel coupons. The AL/BS mix had a surface tension of around 30 mN.m-1, indicating that the presence of alkaline lipase did not interfere in the surface activity properties of the tensoactive component. The antimicrobial activity of the AL/BS mix was determined by minimum inhibitory concentration (MIC) micro-assays. Among all the tested organisms, the presence of the mixture only affected the growth of B. subtilis CCT 2576, B. cereus ATCC 10876 and L. innocua. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Listeria innocua; Microbial biofilm; Minimum inhibitory concentration; Stainless steel surface.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100045
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Characterization of alkaline xylanases from Bacillus pumilus BJM
Duarte,Marta Cristina Teixeira; Pellegrino,Ana Carolina Alcazar; Portugal,Edilberto Princi; Ponezi,Alexandre Nunes; Franco,Telma Teixeira.
Alkaline xylanases produced by four different strains of Bacillus pumilus were characterized. The optimal pH and temperature were pH 9.0 and 60ºC for strain 13a, and pH 8.0 and 55ºC for strains 5(2), 5(14), and 4a. Under these conditions the following activities were found after 10 min in the presence of 1% xylan (birchwood): 328 U.ml-1, 131 U.ml-1, 90 U.ml-1, and 167 U.ml-1, respectively, for the four strains. The enzymes were stable at 40ºC, with 40% of the xylanase activity remaining after 2 hours for the enzymes of strain 5(2) and 60% for the other three strains. Stability at 50ºC was improved by addition of glycerol. Taking into account the conditions under which kraft pulps are bleached during the manufacture of paper, xylanases from B. pumilus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylan xylanase; B pumilus kraft pulp bleaching.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200005
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Control of Colletotrichum gloeosporioides (penz.) Sacc. In yellow passion fruit using Cymbopogon citratus essential oil BJM
Anaruma,Nina Duarte; Schmidt,Flávio Luís; Duarte,Marta Cristina Teixeira; Figueira,Glyn Mara; Delarmelina,Camila; Benato,Eliane Aparecida; Sartoratto,Adilson.
The use of antibiotics in agriculture is limited when compared to their applications in human and veterinary medicine. On the other hand, the use of antimicrobials in agriculture contributes to the drug resistance of human pathogens and has stimulated the search for new antibiotics from natural products. Essential oils have been shown to exert several biological activities including antibacterial and antifungal actions. The aim of this study was to determine the activity of 28 essential oils from medicinal plants cultivated at CPMA (Medicinal and Aromatic Plants Collection), CPQBA/UNICAMP, against Colletotrichum gloeosporioides (Penz.) Sacc., the anthracnose agent in yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg), as well as evaluating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yellow passion fruit; Colletotrichum gloeosporioides; Anthracnose; Antimicrobial activity; Minimal inhibitory concentration.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100012
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Purification and Partial characterization of manganese peroxidase from Bacillus pumilus AND Paenibacillus sp. BJM
Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina.
The production of manganese peroxidase (MnP) from Bacillus pumilus and Paenibacillus sp. was studied under absence and presence of the inducers indulin AT, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. Indulin AT increased the activity of B. pumilus MnP up to 31.66 U/L after 8 h, but no improve was observed for Paenibacillus sp., which reached maximum activity (12.22 U/L) after 20 h. Both MnPs produced by these microorganisms were purified in phenyl sepharose resin and the proteins from crude extracts were eluted in two fractions. However, only the first fraction of each extract exhibited MnP activities. Tests in different pH and temperature values, from pH 5.0 to pH 10.0 and 30 ºC to 60 ºC, respectively, were carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Manganese peroxidase; Purification; Characterization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400012
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Use of Bacillus pumilus CBMAI 0008 and Paenibacillus sp. CBMAI 868 for colour removal from paper mill effluent BJM
Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina.
Bacillus pumilus and Paenibacillus sp. were applied on the paper mill effluent to investigate the colour remotion. Inocula were individually applied in effluent at pH 7.0, 9.0 and 11.0. The real colour and COD remotion after 48h at pH 9.0 were, respectively, 41.87% and 22.08% for B. pumilus treatment and 42.30% and 22.89% for Paenibacillus sp. Gel permeation chromatography was used to verify the molar masses of compounds in the non-treated and treated effluent, showing a decrease in the compounds responsible for the paper mill effluent colour.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Colour removal; Paper mill effluent.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200027
Registros recuperados: 5
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