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Registros recuperados: 158
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A peculiar behaviour for cell death induced by weak carboxylic acids in the wine spoilage yeast Zygosaccharomyces bailii IPB - Escola Superior Agrária
Estevinho, Leticia M.; Côrte-Real, Manuela; Leão, Cecília.
In glucose-grown cells of Zygosaccharomyces bailii, ISA 1307 acetic acid and other carboxylic acids enhanced death. The effects were much lower than those described for Saccharomyces cerevisiae, being only detectable at higher acid concentrations. In Z. bailii, acetic acid and other weak acids also induced intracellular acidification, but this effect was less pronounced than that of death and no relationship was found with death enhancement. The results suggested that in Z. bailii, unlike S. cerevisiae, intracellular acidification induced by weak acids is less pronounced and appears not to have a significant role in death at the temperature range used.
Tipo: Article Palavras-chave: Saccharomyces-Cerevisiae; Acetic-Acid; Intracellular PH; Enthalpy Death; Ethanol; Wine.
Ano: 1999 URL: http://hdl.handle.net/10198/4397
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A survey of the in vitro antifungal activity of heather (Erica sp.) organic honey IPB - Escola Superior Agrária
Feás, Xesús; Estevinho, Leticia M..
Monofloral heather (Erica sp.) honey samples (n = 89), harvested in Portugal according to European organic beekeeping rules, were analyzed to test their antifungal effect against Candida albicans, Candida krusei, and Cryptococcus neoformans. A synthetic honey solution was also tested to determine antifungal activity attributable to sugars. The specific growth rate (l) values showed that growth of all the yeasts was reduced in the presence of honey. The honey concentration (% wt/vol) that inhibited 10% of the yeast growth (Xmin) was 13.5% for C. albicans, 20.5% for C. krusei, and 17.1% for C. neoformans. The respective concentrations of heather honey and synthetic honey in the C. krusei culture medium above 60% (wt/vol) that inhibited 90% of the yeast...
Tipo: Article Palavras-chave: Antifungal effects; Erica species.
Ano: 2011 URL: http://hdl.handle.net/10198/6336
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Actividade antifúngica de extractos fenólicos do mel de Trás-os-Montes IPB - Escola Superior Agrária
Barros, Susana; Calhelha, Ricardo C.; Estevinho, Leticia M..
Tipo: ConferenceObject
Ano: 2005 URL: http://hdl.handle.net/10198/5632
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Actividade antimicrobiana de cogumelos silvestres comestíveis: Lactarius deliciosus, Sarcodon imbricatus e Tricholoma portentosum IPB - Escola Superior Agrária
Barros, Lillian; Calhelha, Ricardo C.; Vaz, Josiana A.; Ferreira, Isabel C.F.R.; Baptista, Paula; Estevinho, Leticia M..
Actualmente a prevalência de doenças infecciosas é um problema à escala mundial, exigindo uma intensa pesquisa de novos agentes antimicrobianos activos contra microrganismos patogénicos. A introdução de produtos naturais para este fim tem suscitado muito interesse nos últimos anos. Os cogumelos para além de reconhecido valor nutricional possuem benefícios medicinais, nomeadamente no tratamento contra cancro de estômago, esófago e pulmões. Tanto o corpo frutificante como o micélio contem compostos com actividade anti bacteriana e antifúngica.
Tipo: ConferenceObject
Ano: 2006 URL: http://hdl.handle.net/10198/3732
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Actividade antimicrobiana de cogumelos silvestres comestíveis: Lactarius deliciosus, Sarcodon imbricatus e Tricholoma portentosum IPB - Escola Superior Agrária
Barros, Lillian; Calhelha, Ricardo C.; Vaz, Josiana A.; Ferreira, Isabel C.F.R.; Baptista, Paula; Estevinho, Leticia M..
Actualmente a prevalência de doenças infecciosas é um problema à escala mundial, exigindo uma maior investigação de novos agentes antimicrobianos activos contra microorganismos patogénicos, Portanto a pesquisa de novos produtos com propriedades antimicrobianas tem-se tornado um grande domínio.
Tipo: ConferenceObject
Ano: 2007 URL: http://hdl.handle.net/10198/3554
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Actividade antimicrobiana de Lactarius deliciosus e Lacarius piperatus em diferentes fases de maturação IPB - Escola Superior Agrária
Barros, Lillian; Ferreira, Edna; Alves, Tânia; Baptista, Paula; Estevinho, Leticia M.; Ferreira, Isabel C.F.R..
Tipo: ConferenceObject
Ano: 2008 URL: http://hdl.handle.net/10198/3474
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Actividade antioxidante de produtos da colmeia IPB - Escola Superior Agrária
Morais, Margarida; Moreira, Leandro; Pereira, Ana Paula; Estevinho, Leticia M..
A apicultura apresenta-se em Portugal como uma actividade que é necessário incentivar tanto pela produção de mel de qualidade, constituindo uma fonte de rendimento da população, quer pela produção de outros produtos da colmeia, tais como, própolis e pólen. O mel é um produto natural produzido pelas abelhas a partir do néctar de plantas, sendo o produto maioritário da colmeia. O própolis e o pólen são produtos secundários da colmeia, pouco valorizados no nosso país, provavelmente devido à escassez de estudos relativos à sua composição e propriedades farmacológicas. O própolis é uma substância recolhida de determinadas partes da planta. O pólen é o conjunto dos minúsculos grãos recolhidos nas flores pelas abelhas. Estes três produtos apresentam uma...
Tipo: ConferenceObject
Ano: 2009 URL: http://hdl.handle.net/10198/4673
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An electronic tongue for honey classification IPB - Escola Superior Agrária
Dias, L.G.; Peres, António M.; Vilas-Boas, Miguel; Rocha, Maria A.; Estevinho, Leticia M.; Machado, A.A.S.C..
An electronic tongue system was developed based on 20 all-solid-state potentiometric sensors and chemometric data processing, with polymeric membranes applied on solid conducting silver-epoxy supports and a Ag=AgCl reference electrode. The sensor array was applied to 52 commercial honey samples obtained randomly from different regions of Portugal. These samples were analysed independently for their pollen profiles by biological techniques and the data collected with the tongue were evaluated for discrimination of the samples with multivariate statistical methods (principal component analysis and linear discriminant analysis), to investigate whether the device may provide an analytical alternative for classification of honey samples with respect to pollen...
Tipo: Article Palavras-chave: Honey; Pollen; Electronic tongue; Multivariate analysis.
Ano: 2008 URL: http://hdl.handle.net/10198/1027
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Análise sensorial de vários tipos de hidromel IPB - Escola Superior Agrária
Gomes, Teresa; Dias, Teresa; Andrade, João Verdial; Cadavez, Vasco; Morais, Jorge Sá; Ramalhosa, Elsa; Estevinho, Leticia M..
Tipo: ConferenceObject
Ano: 2011 URL: http://hdl.handle.net/10198/5618
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Answer from the yeasts Zygosaccharomyces bailii and Saccharomyces cerevisiae to the presence of fungicides: benomyl and cimoxamil IPB - Escola Superior Agrária
Ratão, Isabel; Estevinho, Leticia M..
Sometimes it was detected residues of fungicides in must. These products cause a small inhibitory effect of fermentation. Neverless, when applied 8 a 15 days before the harvest, it could affect negatively the fermentation.
Tipo: Other
Ano: 1998 URL: http://hdl.handle.net/10198/5566
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Antifungal activity of diarylamines in the benzo[b]thiophene series and SAR IPB - Escola Superior Agrária
Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Barbosa, Sandra; Estevinho, Leticia M.; Queiroz, Maria João R.P..
Several diarylamines la-f and 2 were prepared using palladium-catalyzed C-N coupling of electron deficient or rich benzo[b]thienylbromides with aromatic amines. Using 2-aminopyridine a one pot C-N coupling and intermolecular cyclization involving the nitrogen of the pyridine, with lost of ethanol, occurred giving compound 3.
Tipo: ConferenceObject
Ano: 2004 URL: http://hdl.handle.net/10198/3729
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Antifungal effect of lavender honey against Candida albicans, Candida krusei and Cryptococcus neoformans IPB - Escola Superior Agrária
Estevinho, Leticia M.; Afonso, Sílvia Marlene Esteves; Feás, Xesús.
Monofloral lavender honey samples (n=30), were analyzed to test antifungal effect against Candida albicans, Candida krusei, and Cryptococcus neoformans. The specific growth rates (μ) showed that all the yeast growths were reduced in the presence of honey. The honey concentration (% w/v) that inhibited 10% of the yeasts growth (Xmin) ranged from 31.0% (C. albicans), 16.8% (C. krusei) and 23.0% (C. neoformans). A synthetic honey solution was also tested to determine antifungal activity attributable to sugars. The presence of synthetic honey in the C. krusei culture medium at concentrations above 58.0% (w/v) was established as Xmin, while C. albicans and C. neoformans were more resistant, since Xmin values were not reached over the ranged tested (10–60%,...
Tipo: Article Palavras-chave: Antifungal; Lavender.
Ano: 2011 URL: http://hdl.handle.net/10198/5840
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Antimicrobial activity and bioactive compounds of portuguese wild edible mushrooms methanolic extracts IPB - Escola Superior Agrária
Barros, Lillian; Calhelha, Ricardo C.; Vaz, Josiana A.; Ferreira, Isabel C.F.R.; Baptista, Paula; Estevinho, Leticia M..
The antimicrobial properties of phenolic extracts of Portuguese wild edible mushroom species (Lactarius deliciosus, Sarcodon imbricatus and Tricholoma portentosum) against pathogens were investigated. The minimal inhibitory concentrations (MICs) were evaluated for the entire mushroom, the cap and the stipe, separately; the portion of the mushroom used proved to be influenced in the results obtained, which are directly correlated with the content of total phenols and flavonoids in the extracts. The growth of Grampositive bacteria (Bacillus cereus, B. subtilis,) was well inhibited by these mushrooms, while Escherichia coli (Gramnegative bacteria) was resistant. The study on the antifungal effect of these mushrooms revealed that Candida albicans and...
Tipo: Article Palavras-chave: Wild edible mushrooms; Bioactive compounds; Antimicrobial activity.
Ano: 2007 URL: http://hdl.handle.net/10198/829
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Antioxidant activity of honey samples from Trás-os-Montes and inhibitory effect of hydrogen peroxide on pathogenic yeast growth IPB - Escola Superior Agrária
Pereira, A.P.; Ferreira, Isabel C.F.R.; Estevinho, Leticia M..
Honey has been used since ancient times in the treatment of respiratory infections and to heal wounds. These effects are related to its physical and chemical properties. The major antibacterial factor in honey is hydrogen peroxide, which is produced by glucose oxidase originating from hypopharyngeal glands of honey bees, and by catalase, which a originates from pollen
Tipo: ConferenceObject
Ano: 2007 URL: http://hdl.handle.net/10198/3700
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Antioxidant activity of Portuguese honey samples IPB - Escola Superior Agrária
Aires, Edmur; Ferreira, Isabel C.F.R.; Estevinho, Leticia M..
Reactive oxygen species are known to be implicated in many cell disorders and in the development of many diseases including cardiovascular diseases, atherosclerosis, cataracts, chronic inflammation, or neurodegenerative diseases such as Alzheimer's or Parkinson's disease
Tipo: ConferenceObject
Ano: 2008 URL: http://hdl.handle.net/10198/5855
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Antioxidant activity of portuguese honey samples: different contribution of the entire honey and phenolic extract IPB - Escola Superior Agrária
Ferreira, Isabel C.F.R.; Aires, Edmur; Barreira, João C.M.; Estevinho, Leticia M..
The antioxidant activity of Portuguese honeys was evaluated considering the different contribution of entire samples and phenolic extracts. Several chemical and biochemical assays were used to screen the antioxidant properties of entire honeys with different colour intensity and phenolic extracts: reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity, and inhibition of lipid peroxidation using the b-carotene linoleate model system and the thiobarbituric acid reactive substances (TBARS) assay. The amounts of phenols, flavonoids, ascorbic acid, b-carotene, lycopene and sugars present in the samples were also determined. The highest antioxidant contents and the lowest EC50 values for antioxidant activity were obtained in the dark...
Tipo: Article Palavras-chave: Honey; Antioxidants; Phenolics; Scavenging activity; Peroxidation inhibition; Discriminant analysis.
Ano: 2009 URL: http://hdl.handle.net/10198/2648
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Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey IPB - Escola Superior Agrária
Estevinho, Leticia M.; Pereira, A.P.; Moreira, Leandro; Dias, L.G.; Pereira, Ermelinda.
Phenolic compounds of dark and clear honeys from Trás-os-Montes of Portugal were extracted with Amberlite XAD-2 and evaluated for their antioxidant and antimicrobial activities. The antioxidant effect was studied using the in vitro test capacity of scavenge the 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical and of reducing power of iron (III)/ferricyanide complex. The antimicrobial activity was screened using three Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, Staphylococcus lentus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli). The results obtained from the partial identification of honey phenolic compounds by high-performance liquid chromatography with a diode array...
Tipo: Article Palavras-chave: Honey; Phenolic compounds; Antioxidant activity; Antimicrobial activity.
Ano: 2008 URL: http://hdl.handle.net/10198/2942
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Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal IPB - Escola Superior Agrária
Moreira, Leandro; Dias, L.G.; Pereira, J.A.; Estevinho, Leticia M..
Pollen analysis, total phenols content and antioxidant activity were studied for the first time in Portuguese propolis samples from Bornes and Fundão regions. Total phenols content was determined by colorimetric assay and their amount was of 329 mg/g of GAE in Bornes sample and 151 mg/g of GAE in Fundão propolis. The antioxidant capacity of propolis extracts was assessed through the scavenging effects on DPPH (2,2- diphenyl-1-picrylhydrazyl) and reducing power of iron (III)/ ferricyanide complex assays. A concentration- dependent antioxidative capacity was verified in DPPH and reducing power assays. Low values of EC50 on DPPH scavenging assay were obtained for Bornes and Fundão propolis (of 6.22 lg/mL and 52.00 lg/mL, respectively). For reducing power the...
Tipo: Article Palavras-chave: Propolis; Polinic analysis.
Ano: 2008 URL: http://hdl.handle.net/10198/4432
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Aplicação de uma língua electrónica na classificação de méis monoflorais IPB - Escola Superior Agrária
Morais, L.; Dias, L.G.; Peres, António M.; Rocha, A.C.; Estevinho, Leticia M.; Machado, A.A.S.C..
Analisou-se o perfil polínico de 51 amostras de mel nacional. Os pólens mais frequentes no mel são originários das plantas dos géneros Lavandula, Cistus, Echium, Erica, Castanea, Thymus, Prunus, Cytisus, Carduus, Trifolium e Citrus. Considerando a classificação de mel monofloral de Lavandula (pólen predominante superior a 15%), de Erica e de Echium (pólen predominante superior a 45%) seleccionaram-se 38 méis monoflorais: 18, 9 e 11 destes méis foram classificados como méis monoflorais de Lavandula, Erica e Echium, respectivamente. Contudo há méis que podem ser classificados como mel monofloral de dois géneros de pólen (Lavandula-Echium, Erica-Lavandula, Echium-Lavandula). Os méis monoflorais foram analisados com um sistema de multi-sensores...
Tipo: Article Palavras-chave: Mel; Pólen; Língua electrónica; Análise multivariada.
Ano: 2007 URL: http://hdl.handle.net/10198/3031
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Avaliação da actividade biológica do mel IPB - Escola Superior Agrária
Douro, Rute; Estevinho, Leticia M..
Anexo
Ano: 2007 URL: http://hdl.handle.net/10198/5628
Registros recuperados: 158
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