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Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing Biocell
Imani,Rana; Hojjati Emami,Shahriar; Fakhrzadeh,Hossein; Baheiraei,Nafiseh; Sharifi,Ali M.
The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of regeneration. Preparation of cell aggregates is one of the important steps in 3D tissue engineering technology, particularly in organ printing. Two simple methods, hanging drop (HD) and conical tube (CT) were utilized to prepare cell aggregates. The size and viability of the aggregates obtained at different initial cell densities and pre-culture duration were compared. The proliferative ability of the cell aggregates and their ability to spread in culture plates were also investigated. In both methods, the optimum average size of the aggregates was less than 500 µm. CT aggregates were smaller than HD aggregates....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tissue engineering; Hanging drop; Conical tube; Bioprinting.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100003
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