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Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.) Electron. J. Biotechnol.
Tang,Yue Yi; Wang,Chuan Tang; Yang,Guan Pin; Feng,Tong; Gao,Hua Yuan; Wang,Xiu Zhen; Chi,Xiao Yuan; Xu,Ya Long; Wu,Qi; Chen,Dian Xu.
To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known...
Tipo: Journal article Palavras-chave: Chilling response; Peanut; Real-time quantitative PCR; Suppression subtractive hybridization.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005
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