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Registros recuperados: 9
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Characterization of 41 microsatellite loci developed from the genome sequence of the poplar rust fungus, Melampsora larici-populina Inra
Xhaard, C.; Andrieux, A.; Halkett, F.; Frey, P..
Forty-one microsatellite markers were developed for the poplar rust fungus Melampsora larici-populina. The genome sequence of M. larici-populina strain 98AG31 was screened in silico for microsatellite loci using Magellan software. Thirty-nine loci, evaluated on a sexual population of 35 M. larici-populina isolates sampled on larch trees in the French Alps, were identified to be highly polymorphic. These markers revealed two to 34 alleles, with an average of 7.23 alleles per locus. The estimated heterozygosity ranged from 0.029 to 0.958 across the 41 microsatellite loci. These markers will be highly useful for population genetics studies of M. larici-populina.
Tipo: Journal Article Palavras-chave: BASIDIOMYCETE; MELAMPSORA LARICI-POPULINA; POPLAR RUST FUNGUS; GENOME SEQUENCE; MICROSATELLITES; M13-TAILED PRIMER METHOD.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e8fd96fd&uri=/notices/prodinra1/2010/10/
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Comparison of genetic and virulence diversity of Melampsora larici-populina populations on wild and cultivated poplar and influence of the alternate host Inra
Gérard, P.R.; Husson, C.; Pinon; Frey, P..
The aims of this study were, first, to compare the genetic and virulence diversity between populations of the rust fungus Melanipsora larici-populina on wild and cultivated poplar stands and, second, to investigate the influence of the presence of the alternate host of the pathogen, larch, on which its sexual reproduction occurs, on these diversities. Nine French M. larici-populina populations collected from poplar trees in autumn and four. populations collected from larch trees during the following spring were analyzed using both virulence factors and neutral markers. In all, 30 pathotypes were identified within the 13 populations studied. The pathotypic structure clearly distinguished the cultivated stands with high richness and complexity from the wild...
Tipo: Journal Article Palavras-chave: GENETIQUE DES POPULATIONS; ROUILLE; MALADIE DES PLANTES; POUVOIR PATHOGENE; DIVERSITE GENETIQUE; PATHOTYPE; HOTE ALTERNATIF; EPIDEMIOLOGIE BLACK POPLAR; DOMINANT MARKERS; POPULATION GENETICS; RUST EPIDEMICS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007e8ace244&uri=/notices/prodinra1/2007/03/
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Distribution and expression of elicitin genes in the interspecific hybrid oomycete Phytophthora alni. Inra
Ioos, R.; Panabières, F.; Industri, B.; Andrieux, A.; Frey, P..
Phytophthora alni subsp. alni (Paa), P. alni subsp. multiformis (Pam) and P. alni subsp.uniformis (Pau) are responsible for alder disease in Europe. Class I and II elicitin genes patterns of Paa, Pam, Pau and the phylogenetically close species P. cambivora and P.fragariae were studied through mRNA sequencing and 3’ untranslated region (3’UTR)-specific PCRs and sequencing. The occurrence of multiple 3’UTR sequences in association to identical elicitin encoding sequences in Paa indicated duplication/recombination events. The mRNA pattern displayed by Paa demonstrated that elicitin genes from all the parental genomes are actually expressed in this allopolyploid taxon. The complementary elicitin patterns resolved confirmed the possible involvement of Pam and...
Tipo: Journal Article Palavras-chave: HYBRIDATION INTERSPECIFIQUE PHYTOPHTHORA ALNI; PHYTOPHTHORA ALNI SSP ALNI; PHYTOPHTHORA ALNI SSP MULTIFORMIS; PHYTOPHTHORA ALNI SSP UNIFORMIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20079b0f2c9d&uri=/notices/prodinra1/2007/10/
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First report of Melampsora larici-populina on Populus spp. in Eastern North America. Inra
Innes; Marchand; Frey, P.; Bourassa; Hamelin.
Tipo: Journal Article Palavras-chave: MALADIE DES PLANTES; ROUILLE; CHAMPIGNON PHYTOPATHOGENE MELAMPSORA LARICI-POPULINA; POPULUS MAXIMOWICZII X POPULUS BALSAMIFERA.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200742c35492&uri=/notices/prodinra1/2007/03/
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Genetic structure of the poplar rust fungus Melampsora larici-populina : Evidence for isolation by distance in Europe and recent founder effects overseas Inra
Barres, B.; Halkett, F.; Dutech, C.C.; Andrieux, A.; Pinon, J.; Frey, P..
Dispersal has a great impact on the genetic structure of populations, but remains difficult to estimate by direct measures. In particular, gradual and stochastic dispersal are often difficult to assess and to distinguish, although they have different evolutionary consequences. Plant pathogens, especially rust fungi, are suspected to display both dispersal modes, though on different spatial scales. In this study, we inferred dispersal capacities of the poplar rust fungus Melampsora larici-populina by examining the genetic diversity and structure of 13 populations from eight European and two overseas countries in the Northern hemisphere. M. larici-populina was sampled from both cultivated hybrid poplars and on the wild host, Populus nigra. The populations...
Tipo: Journal Article Palavras-chave: ROUILLE; DISPERSION; PLANTE PATHOGÈNE; DIVERSITÉ GENETIQUE MELAMPSORA LARICI-POPULINA; POPULUS NIGRA.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008b7cff2a9&uri=/notices/prodinra1/2009/02/
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INTEGRATION: youth welfare and sustainable development in Switzerland Frontis
Frey, P.; Frutig, S.; Kaltenrieder, U.; Wetzel, J..
In Switzerland with its 7.3 million inhabitants, about 200,000 people are working in the agricultural sector managing 66,000 farms. Between 1990 and 2000, the number of agricultural employees shrunk by 50,000 and more than 22,000 agrarian businesses were abandoned – mostly smallscale farms with less than 3 ha. The Emmental region is a part of Switzerland characterized by a high share of agriculture. Compared to other regions the proportion of rural inhabitants is still relatively high, the decline of farms is therefore below the figures of the rest of Switzerland. Nevertheless, as an effect of the structural change also in rural regions, the remaining farmers depend more and more on additional incomes and are looking for extra work in different branches....
Tipo: Conference proceedings
Ano: 2005 URL: http://library.wur.nl/ojs/index.php/frontis/article/view/1269
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Obligate biotrophpy features unraveled by the genomic analysis of rust fungi Inra
Duplessis, S.; Cuomo, CA; Lin, YC; Aerts, A.; Tisserant, E.; Veneault-Fourrey, C.; Joly, DL; Hacquard, S.; Amselem, J.; Cantarel, B.l; Chin, R.; Coutinho, P.M.; Feau, N.; Field, M.; Frey, P.; Gelhaye, É.; Goldberg, J.; Grabherr, M.G.; Kodira, C.D.; Kohler, A.; Kues, U.; Lindquist, E.A.; Lucas, M; Mago, R.; Mauceli, E.; Morin, E.; Murat, C.; Pangilinan, J.L.; Park, R.; Pearson, M.; Quesneville, H.; Rouhier, N.; Sakthikumar, S.; Salamov, A.A.; Schmutz, J.; Selles, B.; Shapiro, H.; Tanguay, P.; Tuskan, G.A.; Henrissat, B.; Van de Peer, Y.; Rouze, P.; Ellis, J.G.; Dodds, P.N.; Schein, J.E.; Zhong, S.; Hamelin, R.C.; Grigoriev, I.V.; Szabo, L.J.; Martin, F..
Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance or suppression of plant innate immunity. We sequenced the 101-Mb genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89-Mb genome of Puccinia graminis f. sp. tritici, the causal agent of wheat and barley stem rust. We then compared the 16,399 predicted proteins of M. larici-populina with the 17,773 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic lifestyle include expanded...
Tipo: Journal Article Palavras-chave: COMPARATIVE GENOMICS; PLANT PATHOGEN; BASIDIOMYCETE; EVOLUTION; RUST EVOLUTION.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20117dba1845&uri=/notices/prodinra1/2011/06/
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Transcript Profiling of Poplar Leaves upon Infection with Compatible and Incompatible Strains of the Foliar Rust Melampsora larici-populina Inra
Rinaldi, C.; Kohler, A.; Frey, P.; Duchaussoy, F; Ningre, F; Couloux, A.; Wincker, P; Le Thiec, D.; Fluch, S.; Martin, F.; Duplessis, S..
To understand key processes governing defense mechanisms in poplar (Populus spp.) upon infection with the rust fungus Melampsora larici-populina, we used combined histological and molecular techniques to describe the infection of Populus trichocarpa X Populus deltoides 'Beaupre' leaves by compatible and incompatible fungal strains. Striking differences in host-tissue infection were observed after 48-h postinoculation (hpi) between compatible and incompatible interactions. No reactive oxygen species production could be detected at infection sites, while a strong accumulation of monolignols occurred in the incompatible interaction after 48 hpi, indicating a late plant response once the fungus already penetrated host cells to form haustorial infection...
Tipo: Journal Article Palavras-chave: OBLIGATE BIOTROPHIC PATHOGEN; EXPRESSED SEQUENCE TAGS; FOR-GENE SPECIFICITY; HYPERSENSITIVE RESPONSE; HYBRID POPLAR; DISEASE RESISTANCE; LEAF RUST; FLAX RUST; AVIRULENCE GENES; GENOMIC SEQUENCE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20072887511d&uri=/notices/prodinra1/2011/04/
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Usefulness of single copy genes containing introns in Phytophthora for the development of detection tools for the regulated species P. ramorum and P. fragariae Inra
Ioos, R.; Laugustin, L.; Schenck, N.; Rose, S.; Husson, C.; Frey, P..
Introns are generally highly polymorphic regions within genes and were proven to be of great interest for discriminating among phylogenetically-close Phytophthora species. Phytophthora ramorum and P. fragariae are considered as quarantine pathogens by the European Union and accurate detection tools are therefore necessary for their monitoring. From introns located in different single copy genes (GPA1, RAS-like, and TRP1), we developed a series of PCR primers specific to P. ramorum and P. fragariae. The specificity of these primers was successfully checked with a wide collection of Phytophthora isolates and a protocol was developed to detect both pathogens directly in infected plant tissues. These genes should be of particular interest for the development...
Tipo: Journal Article Palavras-chave: INTRON; DIAGNOSTIC CONFIRMATION TEST; DEGENERATE PRIMERS; DIAGNOSIS; PCR.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007ad0184e&uri=/notices/prodinra1/2007/03/
Registros recuperados: 9
Primeira ... 1 ... Última
 

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