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Evaluation of passive immunity transfer against G6P[1] rotavirus in Holstein Calves by ELISA. Repositório Alice
ROCHA, T. G.; SILVA, K. R.; SILVA, F. D. F.; ALFERI, A. A.; SILVA, D. G.; MONTASSIER, H. J.; BUZINARO, M. da G.; GREGORI, F.; ZAFALON, L. F.; FAGLIARI, J. J..
The main strategy to prevent bovine rotavirus group A (RVA) diarrhea in calves is to vaccinate late-term dams aiming to enhance passive immunity transfer of specific immunoglobulins against the virus. This study aimed to evaluate influence of parity in titers of immunoglobulin G (IgG), IgG1 and IgM in serum and colostrum of vaccinated or unvaccinated Holstein cows and in serum of its calves, associated with monitoring for RVA diarrhea in calves. Cows and its calves were allotted into groups according to parity and vaccination (primiparous/multiparous; vaccinated/unvaccinated) and serum and colostrum samples of cows were taken as well as serum and fecal samples of its calves. Parturition influenced colostral titers of IgG and IgG1, which were higher in...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bovine group A rotavirus; Humoral immune response; Dairy calves; Diarrhea; Vaccination.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127571
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Monitoring and molecular characterization of group D rotavirus in Brazilian poultry farms. Repositório Alice
BESERRA, L. A. R.; BERNARDES, N. T. C. G.; BRANDÃO, P. E.; GREGORI, F..
Rotaviruses are etiological agents of diarrhea both in humans and in several animal species. Data on avian Group D rotaviruses (RVD) are scarce, especially in Brazil. We detected RVD in 4 pools of intestinal contents of broilers, layer and broiler breeders out of a total of 111 pools from 8 Brazilian states, representing an occurrence of 3.6%, by a specific RVD RT-PCR targeting the VP6 gene. Phylogenetic tree confirmed that the Brazilian strains belong to group D and 3 of the sequences were identical in terms of amino acid whereas one showed 99.5% identity with the others. The sequences described in this study are similar to other sequences previously detected in Brazil, confirming the conserved nature of the VP6 protein.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Broilers; Ave; Frango; Rotavirus; Rotavirus; Broiler chickens.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1042215
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