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Registros recuperados: 17
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Application of a bioenergetic growth model to larvae of the pearl oyster Pinctada margaritifera L. ArchiMer
Thomas, Yoann; Garen, Pierre; Pouvreau, Stephane.
We applied, for the first time, a dynamic energy budget (DEB) growth model to the larval phase in the pearl oyster Pinctada margaritifera var. cumingii (Linnaeus, 1758) to evaluate the impact of spatio-temporal variation in the atoll lagoon environment on its capacity for development. The specific parameters of the model, which represent ingestion, temperature effect and the relationship between length and biovolume of the larvae, were determined from experiments or taken from the literature. The interpretation of the values of these parameters allowed us to identify the underlying adaptive character trait: P. margaritifera larvae have a good capacity to exploit low food concentrations and a narrow range of thermal tolerance restricted to hydrobiological...
Tipo: Text Palavras-chave: Growth Model; Dynamic Energy Budget; Larva; Pinctada Margaritifera; Atoll Lagoon.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00048/15919/13346.pdf
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Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia ArchiMer
Gueguen, Yannick; Montagnani, Caroline; Joubert, Caroline; Marie, Benjamin; Belliard, Corinne; Tayale, Alexandre; Fievet, Julie; Levy, Peva; Piquemal, David; Marin, Frederic; Le Moullac, Gilles; Ky, Chin-long; Garen, Pierre; Lo, Cedrick; Saulnier, Denis.
Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes...
Tipo: Text Palavras-chave: Pearl oyster; Biomineralization; Pinctada margaritifera.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27840/26049.pdf
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Comparison of growth of mussel, Mytilus edulis, on longline, pole and bottom culture sites in the Pertuis Breton, France ArchiMer
Garen, Pierre; Robert, Stephane; Bougrier, Serge.
Growth of the mussel, Mytilus edulis, was compared for the first time under three culture regimes, longline located in open sea, pole and on-bottom both situated on an intertidal flat, in the Pertuis Breton, on the Atlantic coastline of France. Mussel sampling was performed on a monthly basis over 1 year while monitoring of hydrobiological parameters was conducted on a biweekly basis. Fluctuation of environmental parameters showed a similar pattern on both sea and shore locations with generally higher concentration levels for the intertidal area compared with the open sea, i.e. 4.07 and 3.17 mg 1(-1) for particulate organic matter, 3.16 and 2 mug 1(-1) for chlorophyll-a, 58.43 and 38.72 mg 1(-1) for inorganic-N, respectively. A clear seasonal growth...
Tipo: Text Palavras-chave: Condition index; Emersion; Food resource; Water quality; Growth; Mytilus edulis.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-581.pdf
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Continuous fluorescence recording as a way to improve Pacific oyster (Crassostrea gigas) models of paralytic shellfish toxin accumulation ArchiMer
Baron, Regis; Couedel, Marielle; Joret, Camille; Garen, Pierre; Truquet, Philippe; Masselin, Pierre; Bardouil, Michele; Lassus, Patrick.
A simple system was used to simulate the effect of alternating toxic (paralytic shellfish poisoning toxins) and non-toxic microalgal diets on oyster feeding behaviors and rates of toxin accumulation. These experimental conditions were meant to reflect, to some extent, the incoming and outgoing fluxes of toxic algae observed at the mouth of the Penze estuary (Northern Brittany, France). Physiological and toxicological parameters were estimated based oil fluorescence measurements recorded continuously at the outlet of each experimental tank, which contained a single oyster. Q(tox), this variable describes toxin uptake in oysters, it was used (instead of the toxin ingestion rate): i) in simple graphical analyses, ii) as well as in one- and two-compartment...
Tipo: Text Palavras-chave: Skeletonema costatum; Alexandrium minutum; Fluorescence; Modeling; Kinetics; Toxin uptake; Shellfish toxin; PSP.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1400.pdf
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Development of in situ hybridisation using 16S rRNA gene to monitor black-lip pearl oyster, Pinctada margaritifera, larvae in plankton samples ArchiMer
Thomas, Yoann; Belliard, Corinne; Garen, Pierre; Gueguen, Yannick; Montagnani, Caroline.
In French Polynesia, the black-lip pearl oyster Pinctada margaritifera has been farmed to produce pearls since the 1980s, forming the basis of a major industry. The sustainability of this activity relies on spat collection in the lagoons. However, pearl oyster spat can be difficult to identify for the evaluation of stock variations. It is especially hard to distinguish Pinctada spp. larvae at a very early stage of development. In the present study, a whole- mount in situ hybridisation (ISH) technique was developed to allow the discrimination of larvae of closely-related pearl oyster species found in the French Polynesian atolls. Using specific ribosomal 16S-DNA sequence data, we were able to successfully differentiate between Pinctada margaritifera and...
Tipo: Text Palavras-chave: Whole larvae in situ hybridisation; 16S rRNA; Plankton; Species identification; Pearl oyster; Pinctada margaritifera.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00035/14603/11923.pdf
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Modélisation de la contamination des bivalves par les toxines d'Alexandrium spp. Rapport final ArchiMer
Lassus, Patrick; Amzil, Zouher; Bardouil, Michele; Baron, Regis; Bohec, Madeleine; Bougrier, Serge; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Sechet, Veronique; Bablon, Clotilde; Berard, Jean-baptiste; Carre, Camille; Cheignon, Valerie; Lecamp, Sylvain; Mabit, Marie.
L'objectif général de l'étude est de simuler les épisodes de contamination de certains bivalves (huîtres et moules) par les toxines d'Alexandrium minutum et A. calenella à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à ces espèces. Ce modèle sera ensuite susceptible d'être couplé aux modèles biologiques locaux (estuaire de Penzé et étang de Thau) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer, Université de Montpellier). La première année (2001-2002) était consacrée à la simulation des contaminations en estuaire de Penzé (Alexandrium minulum) et au développement de cultures en masse d'A. calenella de Thau en bioréacteurs.
Tipo: Text
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00156/26711/24786.pdf
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Modélisation de la contamination des huîtres par les toxines d'Alexandrium minutum - Rapport final (2ème année) ArchiMer
Lassus, Patrick; Bardouil, Michele; Baron, Regis; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Joret, Camille.
L'objectif de l'étude est de simuler les épisodes de contamination des huîtres par les toxines d'Alexandrium minufum à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à cette espèce. Ce modèle sera ensuite susceptible d'être couplé au modèle biologique local (estuaire de Penzé) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer).
Tipo: Text
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00156/26712/24787.pdf
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Multi-scale distribution and dynamics of bivalve larvae in a deep atoll lagoon (Ahe, French Polynesia) ArchiMer
Thomas, Yoann; Garen, Pierre; Bennett, Auguste; Le Pennec, Marcel; Clavier, Jacques.
Bivalve larvae and hydrographic parameters were sampled over a range of spatio-temporal scales in a deep atoll lagoon. Bivalve larvae abundances were very high throughout the year: 18,550 m(-3) in average. Larvae were (i) concentrated at mid-depth with nocturnal ascent and diurnal descent, (ii) heterogeneously dispersed at the lagoon scale, (iii) subject to day-to-day variation in abundance and (iv) transferred between different parts of the lagoon providing evidence of intra-lagoonal connectivity. The primacy of physical factors was seen on large spatial scale with the diluting effect of water renewal and transfers by hydrodynamics. On smaller spatial scale, the primacy of biological processes was recognised, with larval swimming activity leading to dial...
Tipo: Text Palavras-chave: Bivalve larvae; Distribution patterns; Vertical migration; Pinctada margaritifera settlement; Deep atoll lagoon; French Polynesia.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21200/20690.pdf
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Paralytic shellfish poison outbreaks in the Penze estuary: Environmental factors affecting toxin uptake in the oyster, Crassostrea gigas ArchiMer
Lassus, Patrick; Baron, Regis; Garen, Pierre; Truquet, Philippe; Masselin, Pierre; Bardouil, Michele; Leguay, Didier; Amzil, Zouher.
Several experiments using a self-regulated system were conducted to define the factors likely to influence the uptake of paralytic shellfish poison (PSP) by oysters in the Penze estuary (France, Brittany). Each 4-day experiment was carried out in a recirculated sea water system using 15 Pacific oysters (Crassostrea gigas) separated from each other and supplied with unfiltered natural seawater containing alternatively toxic (Alexandrium minutum) or non-toxic (Skeletonema costatum) algal diets. The food supply and exposure times to toxic diets were determined according to field studies of the Upstream and downstream movement of patches containing A. minutum. The experimental parameters corresponded roughly to the hydrological conditions generally observed in...
Tipo: Text Palavras-chave: Experimental contamination; Oyster toxicity; Dinophyceae; Toxin; PSP.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-400.pdf
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Ponte et élevage larvaire de bénitier (Tridacna maxima) ArchiMer
Garen, Pierre.
Dans le cadre de l’identification des larves de P.margaritifera », il a été nécessaire de réaliser des pontes d’autres bivalves du lagon et les élevages larvaires de ces espèces. Certains élevages ont été menés à l’écloserie territoriale de Rangiroa. D’autres l’ont été au COP à Vairao, c’est le cas du bénitier Tridacna maxima. Cette note décrit l'obtention des larves de T. maxima et leur morphologie. Elle synthétise les opérations conduites de la collecte des reproducteurs à la fixation des larves.
Tipo: Text
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00173/28382/26698.pdf
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REMOULA 2001. Réseau de croissance moule des Pertuis Charentais. Mars 2001 - mars 2002 ArchiMer
Robert, Stephane; Guilpain, Patrice; Grizon, James; Garen, Pierre; Geairon, Philippe; Razet, Daniel; Faury, Nicole; Seugnet, Jean-luc; Soletchnik, Patrick; Le Moine, Olivier; Taillade, Sylvie.
La mise en place d'un réseau de suivi de croissance des moules dans les Pertuis Charentais s' inscrit dans la démarche de contractualisation de l'IFREMER (Laboratoire Conchylicole de Poitou-Charentes) avec la Section Régionale Conchylicole de Poitou-Charentes. Les intérêts de la profession pour ce réseau sont multiples : l'évolution du classement des zones d'élevage et les baisses de rendement commerciaux annoncées (bouchots) sur les littoraux charentais et vendéen ces dernières années doivent pouvoir être encadrées par des mesures de référence historique, et explicatives. De cette manière, les informations de ce réseau pourront servir d'outil d'aide à la décision pour la profession pour une gestion du milieu optimisée. Les informations obtenues par les...
Tipo: Text Palavras-chave: Réseau de surveillance; REMORA; Moules; Mytilus edulis; Croissance; Mortalité; Pertuis Charentais.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00251/36234/34795.pdf
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REMOULA 2002 - Réseau de Croissance Moule des Pertuis Charentais - Mars 2002 - Mars 2003 ArchiMer
Robert, Stephane; Guilpain, Patrice; Grizon, James; Geairon, Philippe; Razet, Daniel; Garen, Pierre; Faury, Nicole; Seugnet, Jean-luc; Soletchnik, Patrick; Le Moine, Olivier; Goulletquer, Philippe; Taillade, Sylvie.
Le réseau de suivi de croissance des moules dans les Pertuis Charentais a 3 ans d 'existence. L'année 2002 n'est pas une bonne année de croissance. Les mesures de gain de poids moyen journalier saisonnier soulignent un fort retard de croissance pour la période printanière (0,35 en 2002 contre 0,38 en 2001 et 0,69 en 2000). Ce manque de croissance n' est pas récupéré en été et reste équivalent à celui de 2001 (0,30). L'automne montre des résultats très variables, à l'avantage de l'élevage sur filières. La mortalité moyenne (20%) enregistrée en décembre 2002, atteint le même niveau qu 'en 2001, nettement supérieur à celui enregistré à la même date en 2000 ( 14%). Deux indices qualités sont proposés comme référence au remplissage du produit destiné à la vente...
Tipo: Text Palavras-chave: REMOULA; Réseau; Mytilus edulis; Croissance; Mortalité; Production; Qualité; France; Pertuis charentais.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00072/18365/15937.pdf
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Réseau de surveillance des polluants anthropiques dans les lagons de Polynésie française. Rapport final - Septembre 2014. Contrat de projet Etat-Pays ArchiMer
Bernagout, Solene; Bouisset, Patrick; Leclerc, Gael; Rua, Jean; Nohl, Marine; Garen, Pierre; Levy, Peva; Tetaura, Nono-lewis; Poroi, Destremeau; Sham Koua, Manaarii; Liao, Vetea; Bosserelle, Pauline; Planes, Serge; Espiau, Benoit.
La mise en place d’un réseau d’observation de la qualité des eaux lagonaires de Polynésie française, financé par le contrat de projet Etat – Pays, a été initiée mi 2012 pour une durée de deux ans. Durant un à trois semestres la contamination in situ a été suivie sur 6 sites : Vairao et la baie de Phaéton à Tahiti, Moorea (Vaiare), Huahine (Faie), Maupiti et Rangiroa. Le mollusque bioindicateur utilisé est l’huître perlière Pinctada margaritifera. Après transplantation et 4 mois d’immersion sur site, la mesure dans les chairs de la radioactivité gamma n’a pas permis de détecter de radionucléides artificiels, cependant du césium 137 a été mesuré dans les sédiments sur 2 sites. Le plutonium (émetteur alpha) est présent en faibles quantités dans les chairs....
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00249/35975/34499.pdf
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Response of the pearl oyster Pinctada margaritifera to cadmium and chromium: Identification of molecular biomarkers ArchiMer
Gueguen, Yannick; Saulnier, Denis; Santini, Adrien; Magre, Kevin; Garen, Pierre; Bernagout, Solene; Nohl, Marine; Bouisset, Patrick; Helme, Herehia; Planes, Serge; Le Moullac, Gilles.
This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10 μg L− 1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM,...
Tipo: Text Palavras-chave: Pinctada margaritifera; French Polynesia; Cadmium; Chromium; Gene expression; Metallothionein.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00376/48707/49146.pdf
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Spatial and temporal variability of the pico- and nanophytoplankton and bacterioplankton in a deep Polynesian atoll lagoon ArchiMer
Thomas, Y.; Garen, Pierre; Courties, C.; Charpy, L..
Spatial and temporal scales of variability in phyto- and bacterioplankton abundance were investigated in a deep atoll lagoon (Ahe, French Polynesia), Ahe Lagoon showed a classical phytoplankton composition, but its picophytoplankton (Prochlorococcus, Synechococcus and picoeukaryotes) concentrations are higher than the mean for Polynesian atoll lagoons. Phyto- and bacterioplankton descriptors were highly variable, with mean CVs of >20% in most cases: day night, day-to-day, vertical, lagoonal and seasonal. Phytoplankton biomass, which was estimated by in vivo fluorescence, was higher in the deep layer than in the upper layer. This pattern was detected on a day night scale, showing transitory vertical gradients, and through long-term events that were...
Tipo: Text Palavras-chave: Flow cytometry; Picophytoplankton; Nanophytoplankton; Bacterioplankton; Vertical distribution; Day-to-day dynamics; Large-scale heterogeneity; Deep atoll lagoon; French Polynesia.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11425/7963.pdf
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Test du potentiel antisalissure de certaines macromolécules exopolysaccharides pour l'élevage d'huîtres perlières ArchiMer
Garen, Pierre; Tiapari, Jerome.
L'élimination des salissures biologiques recouvrant les huîtres est devenue indispensable dans la plupart des fermes perlières de Polynésie pour maintenir de bonnes conditions d'élevage. Le phénomène massif de biosalissure est dû au développement sur les coquilles des huîtres perlières et sur les structures d'élevage immergées d'espèces épibiontes animales et végétales. Ces nombreuses espèces ont été décrites sur P. margaritifera par Leca (1992). Les bivalves en élevage sont souvent fortement colonisés par les biosalissures qui affectent de manière négative leurs performances (Claereboudt et aL, 1994). Il a été montré que les biosalissures réduisaient également la croissance et la survie des huîtres perlières en élevage, P. fucata (Mohammad, 1976) et P....
Tipo: Text
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00304/41520/40728.pdf
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The limits of morphometric features for the identification of black-lip pearl oyster (Pinctada margaritifera) larvae ArchiMer
Paugam, A; D' Ollone, Christine; Cochard, Jean-claude; Garen, Pierre; Le Pennec, Marcel.
As with most cultivated bivalves, culture of the Tahitian pearl oyster Pinctada margaritifera is particularly dependent on the natural environment, especially for spat supply. The ability to track in real time the abundance and the development of pearl oyster larvae in the plankton would help optimize spat collection in atolls of French Polynesia. However no identification criteria are available for the larvae of several bivalves species present in the lagoons and it is not yet possible to specifically monitor pearl oyster larvae. The aim of this study is to determine the most pertinent morphological identification criteria, to specifically identify the larvae of P. margaritifera and differentiate them from those of three other abundant species: Pinctada...
Tipo: Text Palavras-chave: Morphological features; Larval identification; Pinctada margaritifera.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2531.pdf
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