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Marine microbial biodiversity, bioinformatics and biotechnology (M2B3) data reporting and service standards ArchiMer
Ten Hoopen, Petra; Pesant, Stephane; Kottmann, Renzo; Kopf, Anna; Bicak, Mesude; Claus, Simon; Deneudt, Klaas; Borremans, Catherine; Thijsse, Peter; Dekeyzer, Stefanie; Schaap, Dick M. A.; Bowler, Chris; Gloeckner, Frank Oliver; Cochrane, Guy.
Contextual data collected concurrently with molecular samples are critical to the use of metagenomics in the fields of marine biodiversity, bioinformatics and biotechnology. We present here Marine Microbial Biodiversity, Bioinformatics and Biotechnology (M2B3) standards for "Reporting" and "Serving" data. The M2B3 Reporting Standard (1) describes minimal mandatory and recommended contextual information for a marine microbial sample obtained in the epipelagic zone, (2) includes meaningful information for researchers in the oceanographic, biodiversity and molecular disciplines, and (3) can easily be adopted by any marine laboratory with minimum sampling resources. The M2B3 Service Standard defines a software interface through which these data can be...
Tipo: Text Palavras-chave: Data standard; Marine; Molecular; Biodiversity; Microbial; Bioinformatics; Reporting; Interoperability.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00311/42266/41588.pdf
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The gill chamber epibiosis of deep-sea shrimp Rimicaris exoculata: an in-depth metagenomic investigation and discovery of Zetaproteobacteria ArchiMer
Jan, Cyrielle; Petersen, Jillian M.; Werner, Johannes; Teeling, Hanno; Huang, Sixing; Gloeckner, Frank Oliver; Golyshina, Olga V.; Dubilier, Nicole; Golyshin, Peter N.; Jebbar, Mohamed; Cambon-bonavita, Marie-anne.
The gill chamber of deep-sea hydrothermal vent shrimp Rimicaris exoculata hosts a dense community of epibiotic bacteria dominated by filamentous Epsilonproteobacteria and Gammaproteobacteria. Using metagenomics on shrimp from the Rainbow hydrothermal vent field, we showed that both epibiont groups have the potential to grow autotrophically and oxidize reduced sulfur compounds or hydrogen with oxygen or nitrate. For carbon fixation, the Epsilonproteobacteria use the reductive tricarboxylic acid cycle, whereas the Gammaproteobacteria use the Calvin–Benson–Bassham cycle. Only the epsilonproteobacterial epibionts had the genes necessary for producing ammonium. This ability likely minimizes direct competition between epibionts and also broadens the spectrum of...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00177/28874/28622.pdf
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