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| Fleury, E.; Huvet, A.; Lelong, C.; de Lorgeril, J.; Boulo, V.; Gueguen, Y.; Bachère, E.; Tanguy, A.; Moraga, D.; Fabioux, C.; Lindeque, P.; Shaw, J.; Reinhardt, R.; Prunet, P.; Davey, G.; Lapègue, S.; Sauvage, C.; Corporeau, C.; Moal, J.; Gavory, F.; Wincker, P.; Moreews, F.; Klopp, C.; Mathieu, M.; Boudry, P.; Favrel, P.. |
| Tipo: Journal Article |
Palavras-chave: COQUILLAGE; HUITRE; CRASSOSTREA GIGAS; EXPRESSION DES GENES; BASE DE DONNEES; GIGASDATABASE. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009dfd43c72&uri=/notices/prodinra1/2009/11/ |
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| Yang, Y.; Boze, H.; Chemardin, P.; Padilla, A.; Moulin, G.; Tassanakajon, A.; Pugnière, M.; Roquet, F.; Destoumieux-Garzon, D.; Gueguen, Y.; Bachère, E.; Aumelas, A.. |
| The anti-lipopolysaccharide factor ALF-Pm3 is a 98-residue protein identified in hemocytes from the black tiger shrimp Penaeus monodon. It was expressed in Pichia pastoris from the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter as a folded and N-15 uniformly labeled rALF-Pm3 protein. Its 3D structure was established by NMR and consists of three alpha-helices packed against a four-stranded beta-sheet. The C-34-C-55 disulfide bond was shown to be essential for the structure stability. By using surface plasmon resonance, we demonstrated that rALF-Pm3 binds to LPS, lipid A and to OM(R)-174, a soluble analogue of lipid A. Biophysical studies of rALF-Pm3/LPS and rALF-Pm3/OM(R)-174 complexes indicated rather high molecular sized aggregates, which... |
| Tipo: Journal Article |
Palavras-chave: ANTI-LIPOPOLYSACCHARIDE FACTOR ; LIPOPOLYSACCHARIDE; LIPID A; NMR; STRUCTURE; SEPTIC SHOCK; SURFACE PLASMON RESONANCE ULTRACENTRIFUGATION. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201064bb8a78&uri=/notices/prodinra1/2010/09/ |
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| Boudry, Pierre; Huvet, Arnaud; Lapegue, Sylvie; Sauvage, Christopher; Tanguy, A.; Moraga, D.; Goostrey, A.; Prunet, P.; Boulo, Viviane; Gueguen, Y.; Moal, Jeanne; Fleury, Elodie; Samain, Jean-francois; Mathieu, M.; Favrel, P.. |
| Rationale for EST and genome sequencing of the Pacific oyster: its membership of the Lophotrochozoa, an little studied branch of the Eukaryotes, its worldwide aquacultural importance (4.2 million metric tons, worth 3.5 billion US dollars), its high fecundity, with concomitantly high DNA polymorphism, its use as a biosensor of coastal pollution. In 2004, an international community of biologists, the Oyster Genome Consortium (OGC) led by D. Hedgecock (University of Southern California) and uniting 70 participants from 10 countries, first presented the Pacific oyster Crassostrea gigas as a genome-sequencing candidate to Joint Genome Institute (JGI, USA) (Hedgecock et al., 2005). Besides this project (that remains to be accepted), several EST... |
| Tipo: Text |
Palavras-chave: Characteristics; Genome size; Genome; Crassostrea gigas; Oyster; Genetic. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3414.pdf |
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| Somboonwiwat, K.; Marcos, M.; Tassanakajon, A.; Klinbunga, S.; Aumelas, A.; Romestand, B.; Gueguen, Y.; Boze, H.; Moulin, G.; Bachère, E.. |
| Anti-lipopolysaccharide factors (ALFs), originally characterized from horseshoe crabs, have been recently identified from hemocytes of the black tiger shrimp, Penaeus monodon, by a genomic approach. In order to characterize the properties and biological activities of this immune effector in shrimp, ALFPm3, the most abundant isoform found in P. monodon, was expressed in the yeast Pichia pastoris. Large-scale production in fermentor provided 262 mg/l of recombinant ALFPm3 which was purified to homogeneity by single chromatography step on expanded-bed Streamline SP6XL. The rALFPm3 was further characterized in terms of N-terminal sequencing and mass spectrometry. Anti-microbial assays demonstrated that rALFPm3 has a broad spectrum of anti-fungal properties... |
| Tipo: Journal Article |
Palavras-chave: SHRIMP; CRUSTACEAN; ANTI-LIPOPOLYSACCHARIDE FACTOR; LPS-BINDING; ANTI6mICROBIAL ACTIVITY; VIBRIO; HEMOCYTE. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20092e49f543&uri=/notices/prodinra1/2010/10/ |
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