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Comparison of thin-layer chromatography, spectrofluorimetry and bright greenish-yellow fluorescence test for aflatoxin detection in corn BABT
Ono,Elisabete Yurie Sataque; Silva,Marcelo da; Ribeiro,Ricardo Marcelo Reche; Ono,Mario Augusto; Hayashi,Luciana; Garcia,Glauco Tironi; Hirooka,Elisa Yoko.
In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry, was compared to the thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 corn samples naturally contaminated by the Aspergillus section Flavi. According to the corn processing industry criteria, all the samples were adequate for human and animal consumption by the bright greenish-yellow fluorescence test, but TLC and spectrofluorimetry analysis detected aflatoxins above the maximum tolerated limit (20 µg/kg) in 7 and 8 samples, respectively. Aflatoxins were detected in 16 (40%) corn samples by TLC, with levels ranging from 4.0 to 54.0 µg/kg (mean 19.97 ± 15.97 µg/kg), and in 25 (62.5%) samples by spectrofluorimetry,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thin-Layer Chromatography; Spectrofluorimetry; BGYF; Aflatoxins; Corn; Mycotoxins.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300025
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