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Registros recuperados: 5
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Comparison of genetic and virulence diversity of Melampsora larici-populina populations on wild and cultivated poplar and influence of the alternate host Inra
Gérard, P.R.; Husson, C.; Pinon; Frey, P..
The aims of this study were, first, to compare the genetic and virulence diversity between populations of the rust fungus Melanipsora larici-populina on wild and cultivated poplar stands and, second, to investigate the influence of the presence of the alternate host of the pathogen, larch, on which its sexual reproduction occurs, on these diversities. Nine French M. larici-populina populations collected from poplar trees in autumn and four. populations collected from larch trees during the following spring were analyzed using both virulence factors and neutral markers. In all, 30 pathotypes were identified within the 13 populations studied. The pathotypic structure clearly distinguished the cultivated stands with high richness and complexity from the wild...
Tipo: Journal Article Palavras-chave: GENETIQUE DES POPULATIONS; ROUILLE; MALADIE DES PLANTES; POUVOIR PATHOGENE; DIVERSITE GENETIQUE; PATHOTYPE; HOTE ALTERNATIF; EPIDEMIOLOGIE BLACK POPLAR; DOMINANT MARKERS; POPULATION GENETICS; RUST EPIDEMICS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007e8ace244&uri=/notices/prodinra1/2007/03/
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Rapid in planta detection of Chalara fraxinea by a real-time PCR assay using a dual-labelled probe Inra
Ioos, R.; Kowalski, T.; Husson, C.; Holdenrieder, O..
Chalara fraxinea is a fungus currently threatening ash trees (Fraxinus excelsior) in several European countries. This emerging pathogen was assigned to the EPPO’s alert list and therefore accurate detection and identification tools are needed. Because of its slow growth rate on agar media and the frequent presence of fast-growing saprotrophic fungi within the host tissue, classical isolation techniques are timeconsuming and sometimes inefficient. In this study, we used species-specific polymorphisms observed within the internal transcribed spacer region to design a primer pair and a dual-labelled probe to be used in a real-time PCR assay for the detection of C. fraxinea. The test proved to be specific, based on in silico and in vitro assessments, and could...
Tipo: Journal Article Palavras-chave: PATHOGENICITY; DIAGNOSIS; FRAXINUS EXCELSIOR; DISEASE.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010fafccf75&uri=/notices/prodinra1/2010/10/
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Risk factors for the Phytophthora-induced decline of alder in northeastern France. Inra
Thoirain, B.; Husson, C.; Marcais, B..
A lethal disease of common alder caused by Phytophthora alni, a newhybrid pathogen, has been spreading in Europe since the early 1990s. In2004, we conducted an epidemiological survey in northeastern France todetermine disease frequency and to investigate the impact of environmentalfactors on disease prevalence. Seventy-eight plots in the Rhin-Meuse basin were investigated. The survey was structured to enable criticalexamination of the possible impact of nitrogen pollution of the riverwater on disease prevalence. P. alni-induced alder decline was commonthroughout northeastern France. Altogether, disease was found in 80% ofthe plots containing alder, with 16% of all the alders affected. Strikingdifferences existed between watercourse types. Lower proportions...
Tipo: Journal Article Palavras-chave: BASSIN VERSANT RHIN-MEUSE; FACTEUR DU MILIEU; MALADIE DES PLANTES PHYTOPHTHORA ALNI.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20073c99d54&uri=/notices/prodinra1/2007/05/
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Susceptibility of native French elm clones to Ophiostoma novo-ulmi Inra
Pinon, J.; Husson, C.; Collin, E..
Tipo: Journal Article Palavras-chave: CERATOCYSTIS ULMI; OPHIOSTOMA NOVO-ULMI; ULMUS MINOR; ULMUS GLABRA; CLONE; RESISTANCE AUX ORGANISMES NUISIBLES.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500029486110664&uri=/notices/prodinra1/2009/11/
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Usefulness of single copy genes containing introns in Phytophthora for the development of detection tools for the regulated species P. ramorum and P. fragariae Inra
Ioos, R.; Laugustin, L.; Schenck, N.; Rose, S.; Husson, C.; Frey, P..
Introns are generally highly polymorphic regions within genes and were proven to be of great interest for discriminating among phylogenetically-close Phytophthora species. Phytophthora ramorum and P. fragariae are considered as quarantine pathogens by the European Union and accurate detection tools are therefore necessary for their monitoring. From introns located in different single copy genes (GPA1, RAS-like, and TRP1), we developed a series of PCR primers specific to P. ramorum and P. fragariae. The specificity of these primers was successfully checked with a wide collection of Phytophthora isolates and a protocol was developed to detect both pathogens directly in infected plant tissues. These genes should be of particular interest for the development...
Tipo: Journal Article Palavras-chave: INTRON; DIAGNOSTIC CONFIRMATION TEST; DEGENERATE PRIMERS; DIAGNOSIS; PCR.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007ad0184e&uri=/notices/prodinra1/2007/03/
Registros recuperados: 5
Primeira ... 1 ... Última
 

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