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Fructan degradation and hydrolytic activity in tuberous roots of Viguiera discolor Baker (Asteraceae), a herbaceous species from the cerrado Rev. Bras. Bot.
Degasperi,Maria I.; Itaya,Nair M.; Buckeridge,Marcos S.; Figueiredo-Ribeiro,Rita De Cássia L..
Fructans of the inulin type are the major reserve carbohydrates in tuberous roots of Viguiera discolor, a perennial herb native to the cerrado. Changes in molecular mass of the polymer, followed by releasing free fructose suggested that hydrolysis could be related to the sprouting of the buds after the dormant period, when aerial parts of the plant are naturally absent. Excision of aerial parts resulted in the increase of fructan 1-exohydrolase (1-FEH) activity in tuberous roots after sprouting. 1-FEH was partially purified from this material by binding to ConA-Sepharose and the highest activity was detected at pH 5.4 and between 20 and 40 °C. Values of Km for V. discolor inulin could not be determined since no saturation was observed up to 10%. The study...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asteraceae; Cerrado; Fructan 1-exohydrolase; Inulin.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042003000100003
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Fructans and water suppression on intact and fragmented rhizophores of Vernonia herbacea BABT
Dias-Tagliacozzo,Gláucia M.; Itaya,Nair M.; Carvalho,M. Angela Machado de; Figueiredo-Ribeiro,Rita de Cássia L.; Dietrich,Sonia M. C..
In order to evaluate the involvement of fructans in drought tolerance, experiments were conducted using intact plants and excised rhizophores of Vernonia herbacea. The water content in rhizophores of intact plants was maintained for 30 days when plants were watered every 7 and 15 days, whereas plants remained alive up to 60 days without water. Total fructan, oligo- to polysaccharides ratio and mean molecular mass of polysaccharides increased in these plants indicating depolymerization of median size molecules. In apical fragments of rhizophores kept dry the oligosaccharides increased in relation to polysaccharides one day after excision in treated tissues. This was reflected in the HPLC profile of the oligosaccharides in which the proportion of free...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulin; Environmental stresses; Drought; Fructan metabolism; Reserve organs; Asteraceae.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300005
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