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Registros recuperados: 14
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Authenticating the origin of different shrimp products on the Tunisian markets by PCR/RFLP method ArchiMer
Besbes, Nadia; Jerome, Marc; Berge, Jean-pascal; Sadok, Saloua.
This study describes a polymerase chain reaction using restriction fragment length polymorphism (PCR-RFLP) assay based on the 16S rRNA mitochondrial gene to identify commercial food products of wide range of Penaeidae and Pandalidae shrimp species commercialised in the Tunisian market. Phylogenetic analyses on 16S rRNA mitochondrial gene were used to study the relationships among the considered species. Penaeidae shrimp species was easily differentiated and confirmed by direct sequencing, showing a genetic distance of 0.34 with respect to Pandalidae species. A rapid and reliable PCR method using restriction fragment length polymorphism (RFLP) with three restriction enzymes (HpyCH4III/ MboI / AluI) was optimized for unambiguous differentiation of shrimp...
Tipo: Text Palavras-chave: Shrimps; Species identification; 16S rRNA mtDNA; PCR-RFLP.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00303/41445/40637.pdf
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Gene-associated markers provide tools for tackling illegal fishing and false eco-certification ArchiMer
Nielsen, Einar E.; Cariani, Alessia; Mac Aoidh, Eoin; Maes, Gregory E.; Milano, Ilaria; Ogden, Rob; Taylor, Martin; Hemmer-hansen, Jakob; Babbucci, Massimiliano; Bargelloni, Luca; Bekkevold, Dorte; Diopere, Eveline; Grenfell, Leonie; Helyar, Sarah; Limborg, Morten T.; Martinsohn, Jann T.; Mcewing, Ross; Panitz, Frank; Patarnello, Tomaso; Tinti, Fausto; Van Houdt, Jeroen K. J.; Volckaert, Filip A. M.; Waples, Robin S.; Carvalho, Gary; Albin, Jan Ej; Vieites Baptista, Juan M; Barmintsev, Vladimir; Bautista, Jose M.; Bendixen, Christian; Berge, Jean-pascal; Blohm, Dietmar; Cardazzo, Barbara; Diez, Amalia; Espineira, Montserrat; Geffen, Audrey J.; Gonzalez, Elena; Gonzalez-lavin, Nerea; Guaniero, Ilaria; Jerome, Marc; Kochzius, Marc; Krey, Grigorius; Mouchel, Olivier; Negrisolo, Enrico; Piccinetti, Corrado; Puyet, Antonio; Rastorguev, Sergey; Smith, Jane P; Trentini, Massimo; Verrez-bagnis, Veronique; Volkov, Alexander; Zanzi, Antonella.
Illegal, Unreported and Unregulated fishing has had a major role in the overexploitation of global fish populations. In response, international regulations have been imposed and many fisheries have been 'eco-certified' by consumer organizations, but methods for independent control of catch certificates and eco-labels are urgently needed. Here we show that, by using gene-associated single nucleotide polymorphisms, individual marine fish can be assigned back to population of origin with unprecedented high levels of precision. By applying high differentiation single nucleotide polymorphism assays, in four commercial marine fish, on a pan-European scale, we find 93-100% of individuals could be correctly assigned to origin in policy-driven case studies. We show...
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Ano: 2012 URL: https://archimer.ifremer.fr/doc/00090/20085/17722.pdf
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Genetic diversity analysis of isolates belonging to the Photobacterium phosphoreum species group collected from salmon products using AFLP fingerprinting ArchiMer
Jerome, Marc; Mace, Sabrina; Dousset, Xavier; Pot, Bruno; Joffraud, Jean-jacques.
An accurate amplified fragment length polymorphism (AFLP) method, including three primer sets for the selective amplification step, was developed to display the phylogenetic position of Photobacterium isolates collected from salmon products. This method was efficient for discriminating the three species Photobacterium phosphoreum, Photobacterium iliopiscarium and Photobacterium kishitanii, until now indistinctly gathered in the Photobacterium phosphoreum species group known to be strongly responsible for seafood spoilage. The AFLP fingerprints enabled the isolates to be separated into two main clusters that, according to the type strains, were assigned to the two species P. phosphoreum and P. iliopiscarium. P. kishitanii was not found in the collection....
Tipo: Text Palavras-chave: Photobacterium phosphoreum; AFLP; GyrB-gene; LuxA-gene; Salmon products; Seafood spoilage.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00286/39712/38167.pdf
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Identification of skipjack tuna juveniles based on DNA control region sequences and potential spawning area around reunion island ArchiMer
Nikolic, Natacha; Jerome, Marc; Fonteneau, Alain; Evano, Hugues; Verrez-bagnis, Veronique.
In this study, we confirmed that DNA control region can be used to identify potential spawning area from pelagic juveniles species. We used the mitochondrial DNA control region to identify tuna juveniles predated by common dolphinfish and sampled in surface waters around Reunion Island. These were skipjack juveniles at an early stage (small length and young age). The biological results confirmed the species identification based on DNA analysis. Catch data by longline reveal a higher number of skipjack from October to December around Reunion Island corresponding to the period of our skipjack juvenile sampling
Tipo: Text Palavras-chave: Skipjack tuna; Reunion island; DNA; Age; Juvenile.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00299/40993/42657.pdf
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Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds ArchiMer
Lelievre, Stephanie; Jerome, Marc; Maes, Gregory E.; Vaz, Sandrine; Calaivany, Sachidhanandam; Verrez-bagnis, Veronique.
Maps of the spawning grounds of commercially important fishes are necessary when assessing the level of connectivity between life stages of fishes and for identifying ecologically valuable marine areas. A first step toward mapping the spawning grounds is a reliable and rapid species identification of pelagic fish eggs to assess the spatio-temporal distribution of spawning aggregations. As many species have similar egg sizes and morphology, the molecular validation of visually identified eggs is often essential for the use of such data in fisheries management. In the present study, we developed a rapid 16S rRNA PCR-restriction fragment length polymorphism (RFLP) assay to distinguish between formalin-fixed fish eggs of dab Limanda limanda, flounder...
Tipo: Text Palavras-chave: Fish eggs; PCR-RFLP; 16S rRNA; Geostatistical analyses; Distribution; Spawning grounds.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00059/16999/14536.pdf
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Low mislabeling rates indicate marked improvements in European seafood market operations ArchiMer
Mariani, Stefano; Griffiths, Andrew M.; Velasco, Amaya; Kappel, Kristina; Jerome, Marc; Perez-martin, Ricardo I.; Schroeder, Ute; Verrez-bagnis, Veronique; Silva, Helena; Vandamme, Sara G.; Boufana, Belgees; Mendes, Rogerio; Shorten, Marc; Smith, Cat; Hankard, Elizabeth; Hook, Samantha A.; Weymer, Alice S.; Gunning, Daryl; Sotelo, Carmen G..
Over the span of a decade, genetic identification methods have progressively exposed the inadequacies of the seafood supply chain, revealing previously unrecognized levels of seafood fraud, raising awareness among the public, and serving as a warning to industry that malpractice will be detected. Here we present the outcome of the latest and largest multi-species, transnational survey of fish labeling accuracy to date, which demonstrates an apparent sudden reduction of seafood mislabeling in Europe. We argue that recent efforts in legislation, governance, and outreach have had a positive impact on industry regulation. Coordinated, technology-based, policy-oriented actions can play a pivotal role in shaping a transparent, sustainable global seafood market...
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Ano: 2015 URL: https://archimer.ifremer.fr/doc/00302/41323/40517.pdf
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Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes ArchiMer
Seesao, Y.; Audebert, C.; Verrez-bagnis, Veronique; Merlin, S.; Jerome, Marc; Viscogliosi, E.; Dei-cas, E.; Aliouat-denis, C. M.; Gay, Melanie.
Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
Tipo: Text Palavras-chave: Anisakidae; High-throughput sequencing; Nematode; Pooled DNA extraction; Marine fish; COX2.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00189/30020/28607.pdf
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Multigenerational exposure of the microalga Tetraselmis suecica to diuron leads to spontaneous long-term strain adaptation. ArchiMer
Stachowski-haberkorn, Sabine; Jerome, Marc; Rouxel, Julien; Khelifi, Celia; Rince, Maeva; Burgeot, Thierry.
To investigate the ability of microalgae to develop stable, long-term resistance to herbicides, the marine microalga Tetraselmis suecica was exposed to the herbicide diuron (5 µg/L) for a 43-generation exposure period followed by a 12-generation depuration phase. During the first 25 generations, diuron-exposed cultures showed doubling times ranging from 1.95 to 2.6 days, which was 2 to 2.5-fold longer than control cultures. Between generations 25 and 38, during diuron exposure, two out of the three exposed cultures exhibited a spontaneous drop in doubling time. These results provided evidence of culture adaptation to diuron. To assess persistence of the diuron adaptation observed on growth performance, one of the adapted cultures (D3) was maintained for 12...
Tipo: Text Palavras-chave: Microalgae; Diuron; Multigenerational; Adaptation; Flow cytometry.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00146/25718/23796.pdf
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PCR-RFLP analyses of formalin-fixed fish eggs for the mapping of spawning areas in the Eastern Channel and Southern North Sea ArchiMer
Lelievre, Stephanie; Verrez-bagnis, Veronique; Jerome, Marc; Vaz, Sandrine.
A better knowledge on spawning grounds of principal commercial fishes from North Sea is necessary for their monitoring. Fish egg taxonomic identification based on morphological characters cannot always be carried out effectively. This is particularly the case for a number of Gadidae fish species eggs (i.e. cod, haddock and whiting, and three rockling species) which have the same range of size and for which molecular-based techniques can help improving the accuracy of egg taxonomic identification, and hence the reliability of studies on spawning areas. In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific PCR-RFLP method. Based on the sequence of the cytochrome b gene of reference specimens, a...
Tipo: Text Palavras-chave: Fish eggs; PCR-RFLP; Cytochrome b gene; Geostatistical analyses; Interpolated map.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00005/11613/8259.pdf
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Petromyzon marinus (Petromyzontidae), an unusual host for helminth parasites in western Europe ArchiMer
Gerard, Claudia; Verrez-bagnis, Veronique; Jerome, Marc; Lasne, Emilien.
The sea lamprey Petromyzon marinus, which is among the most phylogenetically ancient vertebrates, is a hematophagous ectoparasite that feeds on vertebrates and is considered vulnerable in Europe but is a pest in the North American Great Lakes. We conducted a literature review of helminth parasites of Petromyzon marinus and investigated postmetamorphic lampreys sampled in rivers and northeast Atlantic coastal waters (western France) during spawning migration. Based on the literature review, 16 helminth taxa have been recorded in Petromyzon marinus, among them 14 in North America but only 2 in Europe, with no species in common between these areas. Specific parasites are lacking, and helminth parasites recorded in Petromyzon marinus are mostly opportunistic...
Tipo: Text Palavras-chave: Sea lamprey; Helminth parasites; Unusual host; Accidental ingestion; Anisakis simplex sensu strict; Nematode.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37964/36040.pdf
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Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes ArchiMer
Sevilla, Rafael G.; Diez, Amalia; Noren, Michael; Mouchel, Olivier; Jerome, Marc; Verrez-bagnis, Veronique; Van Pelt, Hilde; Favre Krey, Laurence; Krey, Grigorios; Bautista, José M..
This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
Tipo: Text Palavras-chave: Teleost; Rhodopsin; PCR primers; Mitochondrial cytochrome b; Fish; DNA barcoding.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3034.pdf
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Restriction fragment length analysis of the cytochrome b gene and muscle fatty acid composition differentiate the cryptic flatfish species Solea solea and Solea aegyptiaca ArchiMer
Boukouvala, Evridiki; Cariani, Alessia; Maes, Gregory E.; Sevilla, Rafael G.; Verrez-bagnis, Veronique; Jerome, Marc; Guarniero, Ilaria; Monios, Georgios; Tinti, Fausto; Volckaert, Filip A.m.; Bautista, José M.; Krey, Grigorios.
Overlapping external morphometric characters easily confound the flatfishes Solea aegyptiaca and Solea solea (Soleidae) in areas of the Mediterranean Sea where both species live in sympatry. This leads to uncertainties in the fisheries and marketing of the species, in addition to misinterpretations in biogeography and conservation studies. Here we describe a simple restriction fragment length-based diagnostic test, which differentiates S. solea from S. aegyptiaca, as well as from other species of the Soleidae family. Furthermore, we show that the two species living in sympatry in the Gulf of Kavala (North Aegean Sea, Greece) present significant qualitative differences in muscle fatty acid composition, a property which can also be used to distinguish the...
Tipo: Text Palavras-chave: Solea solea; Solea aegyptiaca; Species diagnosis; Cytochrome b gene; EcoR V restriction fragment length; White muscle fatty acid composition.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19974/17641.pdf
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Salmon Gravlax Biopreservation: Impact on organoleptic properties, microbial ecosystem and volatilome ArchiMer
Wiernasz, Norman; Chevalier, Frederique; Cardinal, Mireille; Cornet, Josiane; Rohloff, Jens; Jerome, Marc; Chopin, Christine; Donnay-moreno, Claire; Skirnisdottir, S; Pilet, Marie France; Passerini, Delphine; Leroi, Francoise.
Seafood and fishery products are very fragile commodities with a short shelf-life as the consequence of organoleptic and microbiological qualities quick deterioration. Spoilage, resulting from microbial growth and activity, is responsible for up to 25% of food losses in post-harvest and industry1. In this context and to meet the consumer’s demand for minimally processed food, developing mild preserving technologies such as biopreservation, represents a crucial challenge. In this work, we studied the impact of 6 lactic acid bacteria (LAB), selected by Wiernasz et al. (2017)2, used as bioprotective agents on dill Gravlax microbial ecosystem, organoleptic properties and volatilome.
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Ano: 2018 URL: http://archimer.ifremer.fr/doc/00459/57029/58926.pdf
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Tuna labels matter in Europe: Mislabelling rates in different tuna products ArchiMer
Sotelo, Carmen G.; Velasco, Amaya; Perez-martin, Ricardo, I; Kappel, Kristina; Schroeder, Ute; Verrez-bagnis, Veronique; Jerome, Marc; Mendes, Rogerio; Silva, Helena; Mariani, Stefano; Griffiths, Andrew.
Tuna fisheries and processing represent economic activities of paramount importance around the world. Most of these products are traded for human consumption and in general are highly demanded commodities. However, not all tuna products achieve the same market price, some consumers are willing to pay a huge amount of money for certain species (i.e. Japanese market for Bluefin tuna) while other species are rather affordable (i.e. Skipjack tuna), therefore mislabelling has been observed frequently. We collected and analysed 545 tuna samples in six European countries, including fresh, frozen and canned products, and we have investigated whether or not these products were correctly labelled under European and national legislations. We found an overall...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00440/55137/56604.pdf
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