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Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro BJMBR
Li,K.Q.; Jia,S.S.; Ma,M.; Shen,H.Z.; Xu,L.; Liu,G.P.; Huang,S.Y.; Zhang,D.S..
Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fluoride; Periodontitis; Periodontal ligament cells; Mineralization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800601
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