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Registros recuperados: 13
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A unigene catalogue of 5700 expressed genes in cassava Inra
Lopez, C.; Jorge, V.; Piégu, B.; Mba, C.; Cortes, D.; Restrepo, S.; Soto, M.; Laudié, M.; Berger, C.; Cooke, R.; Delseny, M.; Tohme, J.; Verdier, V..
Two economically important characters, starch content and cassava bacterial blight resistance, were targeted to generate a large collection of cassava ESTs. Two libraries were constructed from cassava root tissues of varieties with high and low starch contents. Other libraries were constructed from plant tissues challenged by the pathogen Xanthomonas axonopodis pv. manihotis. We report here the single pass sequencing of 11 954 cDNA clones from the 5' ends, including 111 from the 3' ends. Cluster analysis permitted the identification of a unigene set of 5700 sequences. Sequence analyses permitted the assignment of a putative functional category for 37% of sequences whereas ~16% sequences did not show any significant similarity with other proteins present in...
Tipo: Journal Article Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; EXPRESSION DES GENES; RESSOURCE GENOMIQUE; BACTERIOSE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200758ab2534&uri=/notices/prodinra1/2007/04/
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Amélioration génétique des peupliers Inra
Bastien, C.; Jorge, V.; Dowkiw, A.; Villar, M.; Faivre-Rampant, P..
La variabilité génétique du peuplier lui assure la place de modèle pour une étude fine des gènes impliqués dans des fonctions adaptatives ou de production de bois. Une meilleure connaissance des déterminismes génétiques doit permettre la création de cultivars performants.
Tipo: Journal Article Palavras-chave: AMELIORATION DES ARBRES FORESTIERS; STRATEGIE DE SELECTION; RESISTANCE A LA ROUILLE; ORGANISATION DU GENOME.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007578db49f&uri=/notices/prodinra1/2007/05/
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An EST resource for cassava and other species of Euphorbiaceae Inra
Anderson, J.V.; Delseny, M.; Fregene, M.A.; Jorge, V.; Mba, C.; Lopez, C.; Restrepo, S.; Soto, M.; Piégu, B.; Verdier, V.; Cooke, R.; Tohme, J..
Cassava (Manihot esculenta) is a major food staple for nearly 600 million people in Africa, Asia, and Latin America. Major losses in yield result from biotic and abiotic stresses that include diseases such as Cassava Mosaic Disease (CMD) and Cassava Bacterial Blight (CBB), drought, and acid soils. Additional losses also occur from deterioration during the post-harvest storage of roots. To help cassava breeders overcome these obstacles, the scientific community has turned to modern genomics approaches to identify key genetic characteristics associated with resistance to these yield-limiting factors. One approach for developing a genomics program requires the development of ESTs (expressed sequence tags). To date, nearly 23000 ESTs have been developed from...
Tipo: Journal Article Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; SEQUENCE DE GENES; RESSOURCE GENOMIQUE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007e657bf31&uri=/notices/prodinra1/2007/04/
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Comparative mapping in Salicaceae: a tool for identifying important genes controlling adaptive traits Inra
Jorge, V.; Fabbrini, F.; Faivre-Rampant, P.; Fladung, M.; Gaudet, M.; Lagercrantz, U.; Leplé, J.C.; Morgante, M.; Pakull, B.; Sabatti, M.; Storme, V.; Taylor, G.; Vitacolonna, N.; DeWoody, J.; Bastien, C..
Comparative QTL mapping for adaptive traits among populations and species can lead to the identification of genomic regions conserved over a long evolutionary period and therefore potentially harboring genes of great importance for adaptive processes. At the same time, non-conserved QTLs could be responsible for local adaptation or interspecific differentiation.The availability of Populus as a model system for tree and woody perennial plant biology has largely been driven by the rapid development of genomic and molecular biology resources for this genus, culminating in the completion of a draft sequence of the Populus trichocarpa (black cottonwood) genome. Numerous genetic maps are available in different Populus and Salix species and QTLs for adaptive...
Tipo: Conference Paper Palavras-chave: GENE CANDIDAT; CARTE GENETIQUE; SEQUENCE GENOMIQUE; IDENTIFICATION DE GENES CANDIDATE GENE; GENETIC MAPPING; GENOME SEQUENCE; GENE IDENTIFICATION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20105597576d&uri=/notices/prodinra1/2010/11/
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Genetic architecture of qualitative and quantitative Melampsora larici-populina leaf rust resistance in hybrid poplar: genetic mapping and QTL detection Inra
Jorge, V.; Dowkiw, A.; Faivre-Rampant, P.; Bastien, C..
• In order to elucidate the genetic control of resistance to Melampsora larici-populina leaf rust in hybrid poplars, a Populus deltoides × P. trichocarpa F1 progeny was analysed for qualitative and quantitative rust resistances.• This progeny was evaluated for three components of quantitative resistance (latent period, uredinia number and uredinia size) to seven M. larici-populina strains in controlled conditions, and for one component of field susceptibility (rust colonization on the most infected leaf).• One qualitative resistance locus inherited from P. deltoides, R1, was localized on the genetic map. It segregates 1 : 1 in the F1 progeny and is effective against four of the studied strains. QTL analysis was performed separately on R1 and r1 genotype...
Tipo: Journal Article Palavras-chave: PEUPLIER HYBRIDE; CARTE GENETIQUE; ARCHITECTURE GENETIQUE; DETECTION DE QTL MELAMPSORA LARICI-POPULINA.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007fc50b4e1&uri=/notices/prodinra1/2007/04/
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Genetic linkage maps of Populus alba L. and comparative mapping analysis of sex determination across Populus species Inra
Paolucci, I.; Gaudet, M.; Jorge, V.; Beritognolo, I.; Terzoli, S.; Kuzminsky, E.; Muleo, R.; Scarascia Mugnozza, G.; Sabatti, M..
White poplar (Populus alba L.) is native toEurasia and is unexploited for its growth potential andstress-adaptive mechanisms. A better knowledge of itsgenome will allow for more effective protection and use ofcritical genetic resources. The main objective of this studywas the construction of highly informative P. alba geneticmaps. Two genotypes were selected from contrastingnatural Italian populations and crossed to generate an F1mapping pedigree. Amplified fragment length polymorphismand simple sequence repeat markers were used togenotype 141 F1 individuals. The pseudo-testcross strategywas applied for linkage analysis. The generated mapsshowed good overall colinearity to each other and allowedfor a complete alignment with the 19 haploid chromosomesof the...
Tipo: Journal Article Palavras-chave: DETERMINISME GENETIQUE; DETERMINATION DU SEXE; CARTE DE LIAISON SEX DETERMINATION; LINKAGE MAP; SSR; AFLP.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20107bbd2f12&uri=/notices/prodinra1/2011/01/
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Genetic linkage maps of Populus nigra L. including AFLPs, SSRs, SNPs, and sex trait Inra
Gaudet, M.; Jorge, V.; Paolucci, I.; Beritognolo, I.; Scarascia Mugnozza, G.; Sabatti, M..
Black poplar (Populus nigra L.) is a tree of ecological and economic interest. A better knowledge of P. nigra genome is needed for an effective protection and use of its genetic resources. The main objective of this study is the construction of a highly informative genetic map of P. nigra species including genes of adaptive and economic interest. Two genotypes originated from contrasted natural Italian populations were crossed to generate a F1 mapping pedigree of 165 individuals. Amplification fragment length polymorphism (AFLP), simple sequence repeat (SSR), and single nucleotide polymorphism (SNP) markers were used to genotype 92 F1 individuals, and the pseudo-test-cross strategy was applied for linkage analysis. The female parent map included 368...
Tipo: Journal Article Palavras-chave: CARTE GENETIQUE; IDENTIFICATION DE GENES; DETERMINISME DU SEXE.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20076d63e528&uri=/notices/prodinra1/2009/04/
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Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) Inra
Jorge, V.; Fregene, M.A.; Duque, M.C.; Bonierbale, M.W.; Tohme, J.; Verdier, V..
Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 2 CM 2177-2) led to the construction of the first molecular genetic map of cassava. To better understand the genetics of resistance to CBB, we evaluated individuals of the F1 cross for CBB resistance by controlled greenhouse inoculations and visually assessed symptoms on days 7, 15, and 30 days after inoculation, using a scale where 0 = no disease and 5 = maximum susceptibility. Five Xam strains were used: CIO-84, CIO-1, CIO-136, CIO-295, and ORST X-27. Area under the...
Tipo: Journal Article Palavras-chave: BACTERIOSE.
Ano: 2000 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007bf8f689c&uri=/notices/prodinra1/2007/05/
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Identification of pathotypes of Xanthomonas axonopodis pv. manihotis in Africa and detection of quantitative trait loci and markers for resistance to bacterial blight of Cassava Inra
Wydra, K.; Zinsou, V.; Jorge, V.; Verdier, V..
Cassava suffers from bacterial blight attack in all growing regions. Control by resistance is unstable due to high genotype–environment interactions. Identifying genes for resistance to African strains of Xanthomonasaxonopodis pv. manihotis can support breeding efforts. Five F1 cassava genotypes deriving from the male parent ‘CM2177-2’ and the female parent ‘TMS30572’ were used to produce 111 individuals by backcrossing to the female parent. In all, 16 genotypes among the mapping population were resistant to stem inoculation by four strains of X. axonopodispv. manihotis from different locations in Africa, and 19 groups with differential reactions to the four strains were identified, suggesting that the strains represent different pathotypes. Four genotypes...
Tipo: Journal Article Palavras-chave: PATHOTYPE; BACTERIOSE DETECTION DE QTL.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20076c4dbb22&uri=/notices/prodinra1/2007/06/
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Populus nigra as keystone species able to cope with the ongoing climate change Inra
Villar, M.; Chamaillard, S.; Barbaroux, C.; Bastien, C.; Brignolas, F.; Faivre-Rampant, P.; Fichot, R.; Forestier, O.; Jorge, V.; Rodrigues, S..
Populus nigra L. is one important species of the european alluvial forests, that are protected under Habitats directive 92/43/EEC in Europe. This species is often regarded as good indicator of geomorphological and biological quality of this ecosytem and is an active support of riparian biodiversity. This species is threatened by anthropogenic disturbances and gene introgression, justifying a french and european (EUFORGEN) programme on in situ and ex situ conservation of its genetic resources.In addition, riparian forest ecosystem and trees will face global and local climate modifications this century. Populus nigra, that establishes naturally within or along the active channel and that is strictly dependent of the morphodynamics of the river, will be...
Tipo: Conference Paper Palavras-chave: GENETICS; CLIMATE CHANGE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20104e36fdd8&uri=/notices/prodinra1/2011/02/
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Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis-cassava interaction Inra
Verdier, V.; Restrepo, S.; Mosquera, G.; Jorge, V.; Lopez, C..
Cassava bacterial blight, caused by xanthomonas axonopodis pv. manihotis (Xam), is a widespread disease that affects cassava (Manihot esculenta Crantz). Studies on the pathogen population structure, pathogen diagnosis, identification and expression of plant genes involved in resistance have been carried out. Different molecular techniques were developed to assess the genetic diversity among the Xampopulations. Characterization of Xam population dynamics over time had enable us to determine the different factors that are associated with resistance breakdown and those that influence the genetic structure or virulence phenotypes of the pathogen’s population. Methods for detecting the pathogen in vegetative planting materials and true seeds were developed and...
Tipo: Journal Article Palavras-chave: ETIQUETTE DE SEQUENCE TRANSCRITE; EXPRESSION DES GENES; BACTERIOSE; ADNC; GENE CANDIDAT; DETECTION DE QTL.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20073f223d29&uri=/notices/prodinra1/2007/04/
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Resources and potential for candidate-gene association studies in European black poplar for leaf rust resistance Inra
Jorge, V.; Villar, M.; Paolucci, I.; Bitton, F.; Faivre-Rampant, P.; Dowkiw, A.; Bastien, C..
High levels of variability for partial resistance to Melampsora larici-populina leaf rust in black poplar (Populus nigra L.) across Europe offers unique opportunity to identify favourable alleles through association studies.Nucleotide polymorphism (SNP and indels) within candidate genes has been explored in a discovery panel of 21 French P.nigra genotypes.Phenotyping for field and laboratory resistance components revealed significant diversity at both the intra and inter-metapopulation levels, with a suspected North vs. South geographical contrast.
Tipo: Meeting Paper Palavras-chave: GENE CANDIDAT; RESISTANCE A LA ROUILLE; RESISTANCE FOLIAIRE; POLYMORPHISME NUCLEOTIDIQUE BLACK POPLAR; CANDIDATE GENE; RUST RESISTANCE; LEAF RUST; NUCLEOTIDE POLYMORPHISM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010a6488aee&uri=/notices/prodinra1/2010/09/
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Stratégies raisonnées d'échantillonnage pour capturer la diversité génétique et sa structuration dans les populations naturelles - Application aux mesures de gestion conservatoire. Inra
Brachet, S.; Bastien, C.; Bilger, I.; Buret, C.; Dufour, J.; Grange, J.; Guerin, V.; Jorge, V.; Le Guerroue, B.; Lesage Descauses, M.C.; Leveque, L.; Mardare, I.; Mariette, S.; Musch, B.; Sanchez, L.; Stoeckel, S.; Villar, M.; Santi, F..
La diversité génétique d’une espèce se distribue entre populations et à l’intérieur des populations. Sa prise en compte à ces deux niveaux a une importance majeure pour la mise en place de modes de gestion conservatoires in situ et ex situ. La question principale qui se pose est : quel échantillonnage de la diversité faire, enterme de populations, d’individus et de gènes ? Nous abordons ici cette question, d’un point de vue théorique à travers le éveloppement d’un modèle Monte-Carlo, puis d’un point de vue expérimental sur deux espèces forestières, le merisier et le peuplier noir, à travers une comparaison de la diversité présente en populations naturelles et en collection ex situ. Les deux approches sont ensuite mises en regard l’une de l’autre afin de...
Tipo: Conference Paper Palavras-chave: DIVERSITE GENETIQUE; VARIABILITE INTRAPOPULATION; VARIABILITE INTERPOPULATION; POPULATION NATURELLE; COLLECTION.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20079d370b9b&uri=/notices/prodinra1/2009/06/
Registros recuperados: 13
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