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A leukocyte cryopreservation technique for cytogenetic studies Genet. Mol. Biol.
Duarte,José Maurício Barbanti; Ramalho,Mariney Flávia Pereira Di Tano; Lima,Vera Fernanda Hossepian de; Jorge,Wilham.
Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinez (VYM) solution with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP) as cryopreservatives. The best protocol for freezing was 4°C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still frozen material was placed onto cultivation medium for propagation.
Tipo: Info:eu-repo/semantics/article
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300019
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Chromosome study of Anteaters (Myrmecophagideae, Xenarthra): a preliminary report Genet. Mol. Biol.
Pereira Júnior,Hélio Rubens Jacintho; Jorge,Wilham; Costa,Maria Elvira Loyola Teixeira da.
Anteaters belong to the Order Xenarthra / Family Myrmecophagidae and are the only members without teeth. There are three genera with four living species in the family Myrmecophagidae: Myrmecophaga tridactyla (giant anteater), Tamandua tetradactyla (southern lesser anteater), Tamandua mexicana (northern lesser anteater), and Cyclopes didactylus (silky anteater). The karyotypes of M. tridactyla (2n = 60), T. tetradactyla (2n = 54) and C. didactylus (2n = 64) have already been described. In the present paper, three female and two male specimens of giant anteater and one lesser anteater male were analyzed. The results indicate the existence of a new karyotype in the genus Tamandua, with 2n = 56 chromosomes, which can represent a new lesser anteater species....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anteater; Chromosomes; Myrmecophagidae.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300014
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Cytogenetic and molecular analysis of the Pantaneiro cattle breed PAB
Issa,Érica Cunha; Jorge,Wilham; Sereno,José Robson Bezerra.
The objective of this work was to characterize Pantaneiro cattle genetically through its paternal ancestry by the morphology of the Y chromosome, whether submetacentric or acrocentric, as well as to identify the maternal ancestry through mitochondrial DNA. The karyotype and mitochondrial DNA of 12 bulls of Pantaneiro breed were analyzed. The Y chromosome was analyzed in lymphocyte metaphases and the mitochondrial DNA by diagnosing its haplotype (Bos taurus and Bos indicus). Among Pantaneiro animals analyzed three had a taurine (submetacentric) Y and nine had a zebuine (acrocentric) Y chromosome, suggesting breed contamination by Zebu cattle, once Pantaneiro is considered to be of European origin. The mitochondrial DNA was exclusively of taurine origin,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bos taurus taurus; Bos taurus indicus; Y chromosome; Mitochondrial DNA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001100006
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