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Registros recuperados: 9
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Comparison between deep intracornual artificial insemination (dIAI) and conventional artificial insemination (AI) using low concentration of spermatozoa in beef cattle BABT
Meirelles,Ciro; Kozicki,Luiz Ernandes; Weiss,Romildo Romualdo; Segui,Marcio Saporski; Souza,Antonio; Santos,Ivo Walter dos; Breda,Jose Carlos dos Santos.
This study aimed to compare the pregnancy rate using the conventional artificial insemination (AI) or deep intracornual artificial insemination (DIAI), with low number of spermatozoa (4.0 million sperm) in 270 Nelore cows. The animals were divided in two groups (G: G1 (135 cows) conventional AI was performed (=semen deposition in the uterine body) and in G2 (135 cows) to DIAI, in ipsilateral horn where the dominant follicle in the ovary had previously been detected, by ultrasound examinations. For both the methods, a single artificial insemination was carried out after visual estrus observation, checked three times a day (morning, afternoon and evening). The pregnancy diagnosis after 45 days was conducted by ultrasound. Results showed a better pregnancy...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deep intracornual artificial insemination; Beef cattle; Artificial insemination; Nelore cows.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300006
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Dois diluentes comerciais na criopreservação de espermatozoides do epidídimo de touros Ciência Rural
Bertol,Melina Andrea Formighieri; Weiss,Romildo Romualdo; Fujita,Aline Silva; Kozicki,Luiz Ernandes; Abreu,Ana Claudia Machinski Rangel de; Pereira,João Filipi Scheffer.
A recuperação e a criopreservação de espermatozoides do epidídimo constituem alternativas viáveis para a preservação de material genético de animais valiosos. O objetivo deste estudo foi comparar o desempenho de dois diluentes comerciais Botu-Bov(r) (BB) e Bovimix(r) (BV), sobre a viabilidade pós-descongelação de espermatozoides do epidídimo de touros Tabapuã (Bos taurus indicus) pós-castração. Os espermatozoides foram colhidos da cauda de 20 epidídimos utilizando a técnica de fluxo retrógrado, centrifugados e diluídos com BB ou BV para posterior criopreservação a -196°C. Após a descongelação, as amostras foram avaliadas utilizando a análise computadorizada (CASA) e por análises microscópicas para a determinação da integridade de membranas plasmáticas,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diluente; Viabilidade espermática; Epidídimo; Análise computadorizada do sêmen (CASA).
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000901658
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Embryo transfer in no cycling Crioula and Quarter horse breeds treated with estradiol cipionate and long-acting progesterone BABT
Kaercher,Fernanda; Siqueira,Marlon; Mercadante,Alessandro; Figueiredo,Tatiana; Weiss,Romildo Romualdo; Siqueira,Felipe; Kozicki,Luiz Ernandes.
The aim of this work was to prepare the mares for embryo transfer. In group 1 (G1,treated, n=15), recipient mares in anoestrus or in a transition period were treated with 5.0, 3.0 and 2.0 mg of estradiol cipionate at the days 0, 1 and 2 respectively, beginning at the day of ovulation (DO). From the fourth day on, the mares this group received long-acting progesterone weekly, up to the 120 day of gestation. At D8, the embryo was collected from the donor and transferred to the recipient. At D12, the ultrasonographyc diagnosis of pregnancy was carried out. The control group (G2, not treated, n=20) was formed by cycling recipient mares, displaying ovulation at each 2 to 3 days after the donors mare ovulation. The pregnancy rate was higher (p<0.05) in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo transfer; Cycling and no cycling mares; Long acting progesterone; Estradiol cipionate; Mares recipient.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600009
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Estrus synchronization and Fixed Time Artificial Insemination (FTAI) in dairy buffaloes during seasonal anestrus BABT
Frares,Luciana Ferri; Weiss,Romildo Romualdo; Kozicki,Luiz Ernandes; Santangelo,Renata Prestes; Abreu,Renata Azevedo de; Santos,Ivo Walter do; Dell'aqua Junior,José Antonio; Breda,José Carlos.
The aim of this work was to study estrus synchronization and fixed time artificial insemination (FTAI) in dairy buffaloes during season anestrus. One hundred thirty-nine dairy buffaloes in seasonal anestrus were divided in two groups as G1(n=66) and G2(n=73). The protocols for both the groups were the same until day (D)14:D0 administration of 2.0 mg estradiol benzoate and implantation of progesterone device (P4) for 14 days; D14 removal of P4 plus 150 mg of cloprostenol and 400 IU of equine chorionic gonadotropin. On D16, G1 received 10 mg of buserelin and G2 100 mg deslorelin acetate. On D17, both the groups were submitted to FTAI. Ultrasonographic examinations of ovaries were performed on D0, D14, D16 and D17. Results showed that pregnancy rates in G1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Estrus Synchronization; Seasonal Anestrus; Dairy Buffaloes; Deslorelin; Buserelin; FTAI.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400007
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Impact of Equine Chorionic Gonadotropin Associated with Temporary Weaning, Estradiol Benzoate, or Estradiol Cypionate on Timed Artificial Insemination in Primiparous Bos Indicus Cows BABT
Souza,Andre Luis Bastos; Segui,Marcio Saporski; Kozicki,Luiz Ernandes; Weiss,Romildo Romulado; Abreu,Ana; Bertol,Melina Andreia Formighieri; Oliveira,Danilo Martins Amadori.
The study aimed to determine the impact of equine chorionic gonadotropin (eCG) associated with different timed artificial insemination (TAI) protocols on the pregnancy rate (PR) in Bos indicus cows previously treated with progesterone. Five hundred and fifty-seven primiparous cows were subjected to the following treatments: on day 0 (d0), GeCGTW (group equine Chorionic Gonadotropin+Temporary Weaning;n=178) received 0,558 g intravaginal progesterone (P4)+1.0 mg of estradiol benzoate (EB) (IM); on d8 (P4 removal+0,075 mg D-cloprostenol + 400 IU eCG + TW for 48 h); on d10, TAI + calves return to dam; GeCGEB (group equine Chorionic Gonadotropin+Estradiol benzoate; n=176) the same as GeCGTW without TW + application of 1.0 mg of EB on d9; GeCGEC (group equine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beef catlle; Equine Chorionic Gonadotropin; Fixed time artificial insemination.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100305
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Induction of ovulation in quarter horse mares through the use of deslorelin acetate and human chorionic gonadotrophin (hCG) BABT
Figueiredo,Tatiana; Paiva,Roman; Kozicki,Luiz Ernandes; Kaercher,Fernanda; Weiss,Romildo Romualdo; Santos,Ivo Walter dos; Muradas,Priscilla Ricabone.
The aim this study was to compare two protocols of induction for ovulation by desloreline acetate and hCG in Quarter Horse mares. The choice of the animals was based on the observations by the estrus, by rectal palpation of the ovaries and by ultrassonography of the follicular dynamics. After estrus detection and follicle control, the measurement of the follicles and the classification of uterus were carried out. The animals that had dominant follicle (diameter more than 35 mm) and swollen uterus were used. In these conditions, the mares received hCG or desloreline acetate. Once ovulation occurred, the artificial insemination was carried. Two groups were performed: G1 (20 animals) received 1.5 mg desloreline acetate and G2 (20 animals) received 1700 IU of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovulation; Deslorelin acetate; Human chorionic gonadotrophin; Quarter horse mares.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300012
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Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimários ovinos Ciência Rural
Bergstein-Galan,Tácia Gomes; Weiss,Romildo Romualdo; Barbosa,Thiago Salvati Rodrigues; Kozicki,Luiz Ernandes; Bicudo,Sony Dimas.
ABSTRACT: Preservation and use of spermatozoa that have been recovered after death can extend the use of genetically superior animals. The objective of this study was to evaluate the maximum period for which ovine spermatozoa could be successfully stored in refrigerated dilution medium post-mortem, with or without added seminal plasma. Three samples of spermatozoa collected in an artificial vagina from 10 rams, or from the tails of four epididymes from the same rams at the time of death (G0) and six (G6), twelve (G12), twenty-four (G24) and forty-eight (G48) hours after death were used. After recovery, the spermatozoa were refrigerated at 5°C in either control medium (CM) or control medium plus 20%homologous seminal plasma (SP) and evaluated for 72 hours...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Refrigeration; Epididymis; Post mortem.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000800700
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The effects of intramuscular or intravenous injections of gonadotropin releasing hormone at fixed-time artificial insemination on pregnancy rates of Bos indicus beef cows BABT
Demeterco,Danilo; Henry,Darren Dwayne; Mercadante,Vitor Rodrigues Gomes; Lamb,Graham Cliff; Gaievski,Francisco Romano; Weiss,Bruno; Turbay,Gustavo Nascimento; Segui,Márcio Saporski; Weiss,Romildo Romualdo; Betiol,Melina Andreia Formighieri; Kozicki,Luiz Ernandes.
The effect of an intramuscular versus intravenous administration of gonadotropin releasing hormone (GnRH) at fixed-time AI (FTAI) on the pregnancy rates of crossbred Bos indicus beef cows was evaluated. Pluriparous nursing calv cows (n=120) were synchronized as follows: d 0 cows received a 2.0 mg injection of estradiol benzoate (EB) and insertion of a controlled intravaginal progesterone releasing device containing 0.558 g of progesterone, d 8 removal of the progesterone device , a 0.15 mg injection of prostaglandin F2α (PGF), a 1.0 mg injection of EB, and 400 IU injection of equine chorionic gonadotropin. Fifty-four hr after PGF, all cows were exposed to FTAI and a 0.084 mg injection of GnRH was administered either via Vena caudalis (n=60), or via...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gonadotropin releasing hormone; Fixed-time artificial insemination; Intravenous intramuscular administration.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300008
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Viability of bull spermatozoa collected from the epididymis stored at 18-20°C BABT
Bertol,Melina Andrea Formighieri; Weiss,Romildo Romualdo; Thomaz-Soccol,Vanete; Kozicki,Luiz Ernandes; Fujita,Aline Silva; Abreu,Renata Azevedo de; Green,Kerriel Thandile.
The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p<0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bull; Epididymis; Orchiectomy; Sperm; Room temperature; Viability.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500008
Registros recuperados: 9
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