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Registros recuperados: 23
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Amélioration des productions phytoplanctoniques en écloserie de mollusques : caractérisation des microalgues fourrage ArchiMer
Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie.
in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the...
Tipo: Text Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf
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Application of a digital pattern recognition system to Dinophysis acuminata and D-sacculus complexes ArchiMer
Truquet, Philippe; Lassus, Patrick; Honsell, Giorgio; Le Dean, Loic.
An image discrimination technique was developed to improve specific identification of some toxic Dinophysis cells (marine dinoflagellates involved in diarrhoeic shellfish poisoning), especially the ''acuminata'' and ''sacculus'' groups, which can be present at different ratios in natural sea-water samples collected during toxic episodes. This work was performed with image analysis software SAMBA (TITN Alcatel) using preserved cells directly observed through an inverted microscope and recorded with a video camera before further processing. All morphometric parameters used for discrimination of 7 different species or morphotypes of Dinophysis were tested with discriminant analysis. This study indicates that Dinophysis sp. and D. pavillardi seem well...
Tipo: Text Palavras-chave: Dinophysis sp.; Analyse d'image; Taxonomie; Phytoplancton; Dinophysis sp; Image analysis; Taxonomy; Phytoplankton.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00188/29890/28337.pdf
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Aquaculture of microalgae in New-Caledonia. AMICAL ArchiMer
Coulombier, Noemie; Chim, Liet; Le Dean, Loic; Morezzi, Nicola; Brun, Pierre; Malo, Florent; Nakagawa, Kento; Ducrocq, Manuel; Rivaton, Adrien; Colin, Fabrice.
AMICAL is a partnership program of research and innovation between ADECAL TECHNOPOLE (New Caledonia innovation and technology park) and IFREMER to initiate the development of a microalgae industry in New Caledonia, based on selected local strains. This French oversea territory, located in south Pacific, features many competitive advantages for the development of a blue economy industry (climate, biodiversity hotspot, well developed aquaculture sector...). The project relies on two laboratories (Figure 1) owned and managed by ADECAL TECHNOPOLE with the scientific support of IFREMER: • the Laboratory for MicroAlgae study (LEMA), whose scientific direction is provided by the laboratory of Physiology and Biotechnology of Algae (PBA, IFREMER, Nantes),...
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Ano: 2016 URL: http://archimer.ifremer.fr/doc/00358/46897/46783.pdf
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Aquaculture of Microalgae in New-Caledonia (AMICAL): development of a co2 supply device for intensive microalgae culture. assessment of the device with carbon budget of cultures in microcosm. ArchiMer
Nakagawa, Kento; Coulombier, Noemie; Le Dean, Loic; Brun, Pierre; Chim, Liet.
Aquaculture diversification has been set as a major policy by New Caledonia public authorities. This has been set up to carry by ADECAL Technopole which carries projects in view of developing cultivation marine species native from Caledonian waters. In this context, the development of micro-algal cultures has been identified as a priority. The study presented has been co-funded by Glencore company in New Caledonia and achieved under the program entitled Aquaculture of MIcro-algae in new-CALedonia (AMICAL) and has two objectives: (i) designing a device to bring dissolved CO2 into micro-algae cultures with a minimum release in atmosphere of this green-house gas and (ii) to assess this device by carrying out carbon budget of micro-algae cultures in...
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Ano: 2016 URL: http://archimer.ifremer.fr/doc/00358/46898/46784.pdf
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Culture en continu de Isochrysis galbana affinis Tahiti ArchiMer
Bougaran, Gael; Le Dean, Loic; Lukomska, Ewa; Picquet, Marie-lise; Kaas, Raymond; Muller-feuga, Arnaud.
La prymnésiophycée Isochrysis galbana affinis Tahiti (T -iso) est une micro algue marine classiquement utilisée comme nourriture de larves de mollusques et de proies vivantes de poissons d'aquaculture dans la plupart des écloseries produisant des juvéniles de ces espèces. Aujourd'hui, de nombreuses écloseries de mollusques et de poissons l'utilisent en association avec d'autres espèces telles que Pavlova lutheri, Chaetoceros calcitrans et Skeletonema costatum. Isolée à Tahiti par JL Martin en 1975 (comm. pers.), cette algue s'est rapidement imposée dans la nutrition larvaire des crevettes et des mollusques en raison de sa croissance rapide en conditions tropicales et de ses qualités nutritionnelles, en relation avec sa forte teneur en acides gras...
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Ano: 2001 URL: http://archimer.ifremer.fr/doc/00304/41508/40716.pdf
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Détection automatique par visionique des Dinophysis spp. contenus dans des échantillons d'eau de mer. Premiers résultats ArchiMer
Le Dean, Loic; Gauthier, Franck.
Nous avons réalisé cette étude dans le but de fournir aux agents d'IFREMER chargés de la surveillance des eaux littorales et en particulier de la flore phytoplanctonnique, un outil permettant d'identifier et de compter les cellules de Dinophysis de façon automatique. La visionique nous semblant être une discipline qui se prêtait à ce type de détection, nous avons réalisé une étude de faisabilité d'un système d'analyse d'il}1age appliqué à la reconnaissance et au comptage de Dinophysis. Le capteur utilisé est une caméra J.V.C Tri C.C.D transmettant trois images (rouge - verte - bleue) à un ordinateur compatible A.T.. Celui ci est équipé d'un ensemble de cartes MATROX de numérisation et traitement d'images et d'un coprossesseur arithmétique. Les applications...
Tipo: Text Palavras-chave: Dinophysis; Phytoplancton; Visionique; Reconnaissance de forme; Segmentation; Binarisationcontours.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00136/24714/22768.pdf
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Dinoflagelles toxiques sur les cotes francaises pendant l'ete 1983.Validite du test-souris pour le controle routinier de secteurs mytilicoles contamines par la toxine du Dinophysis. ArchiMer
Marcaillou-le Baut, Claire; Le Dean, Loic; Truquet, Philippe.
Following exceptional outbreak of toxic dinoflagellate species in Vilaine Bay (summer 1983) a survey was carried out: variations in phytoplankton abundance and distribution in water and in mussel guts were followed. In the same time toxicity studies using mouse-test were performed with contaminated shellfishes, and mouse-test calibration was attempted. In spite of sampling and analysis conditions could be open to criticism, results obviously showed relation between shellfishes toxicity and occurrence of responsible species: Dinophysis acuminata.
Tipo: Text Palavras-chave: Brittany; France; ANE; Dinophysis acuminata; Red tides; Toxicity tests; Food poisoning; Biological poisons; Mussel culture.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/publication-1808.pdf
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Etude de la décontamination de moules toxiques (toxines diarrhéiques) en laboratoire et en milieu naturel ArchiMer
Marcaillou-le Baut, Claire; Bardin, B.; Bardouil, Michele; Bohec, Madeleine; Le Dean, Loic; Masselin, Pierre; Truquet, Philippe.
Two studies of depuration, one in laboratory conditions and the other one in an oyster's pond were performed using 2 sets of contaminated mussels with diarrhetic toxins at a high and a low level (3 and 1 M.U.). Depuration kinetics were followed both by the adult mouse test and okadaïc acid analysis. Results are - in the pond, high toxic mussels need about 20 days to become eatable (toxicity level : 0.5 M.U.g-1 digestive glands), -this time decreases when the initial toxicity is low but it increases when mussels are underfed, - a good correlation is observed between the total toxicity and okadaïc acid concentration.
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Ano: 1990 URL: http://archimer.ifremer.fr/doc/00075/18595/16148.pdf
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Etudes préliminaires sur la sensibilité d'organismes planctoniques marins vis à vis de deux insecticides et d'un herbicide ArchiMer
Marcaillou Le Baut, C; Bardouil, Michele; Bocquene, Gilles; Le Dean, Loic; Truquet, Philippe.
This work is the preliminary synthesis of some toxicological tests conducted on three pesticides. This recapitulation would not have been possible without the contribution of our trainees, Misses V. Perles and H. Le Boulicot and MM. M. Falchier and G. Gaty who contributed largely to this study, supervised by the laboratory staff. The obtained results cannot be considered as definitive. The urge to answer the needs did not leave us enough time to fine tune and/or complete the results; for example we still have not determined the lethal dose for 50% of the individuals. [...]
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Ano: 1983 URL: https://archimer.ifremer.fr/doc/1983/rapport-4575.pdf
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Evaluation du potentiel nutritif des eaux de la baie de Vilaine pendant l'été 1987 ArchiMer
Le Dean, Loic; Truquet, Philippe; Lassus, Patrick.
The growth potential study of waters of the Vilaine bay was realized from march to september 1987 and for three sampling stations. The growth of depleted test cells of Skeletonema costatum was estimated and compared from existing nutrients pools for aIl water samples. As a first result it appears that inorganic nitrogen could stimulate the growth during spring while no clear relationship is observed between the algal growth and other hydrological parameters during spring or summer periods. Nevertheless these first results need further corroboration.
Tipo: Text Palavras-chave: Potentiel nutritif; Baie de Vilaine; Skeletonema costatum; Test biologique; Growth potential; Vilaine Bay; S. costatum; Bioassay.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00139/25037/23146.pdf
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Hydrologie et distribution de dinophysis en juin 1988, dans la frange côtière nord-Gascogne ArchiMer
Lassus, Patrick; Truquet, Philippe; Le Dean, Loic; Bardouil, Michele.
During the oceanographic cruise of June 1988, phytoplankton and correlated hydrological parameters were studied in the coastal area between Camaret and Ile d'Oléron. 62 stations were sampled in the 0-30 m layer within the 50 m depth limited coastal area. Observations focused on two toxic dinoflagellate species: Dinophysis spp and Gyrodinium aureolum. Dinophysis spp is more abundant in the Loire/Vilaine area, which is.characterized by high surface Nitrate levels, high 30 m depth Phosphate levels and Chlorophyll a amounts higher than elsewhere during the survey. This particuliar pattern can be related to the Nutrient outputs of Loire and Vilaine Rivers. Max. Dinophysis concentrations were observed in Vilaine bay (very high Phosphate levels) and in Douarnenez...
Tipo: Text Palavras-chave: Phytoplancton; Dinophysis; Gyrodinium aureolum; Hydrologie; Sels nutritifs; Campagne côtière; Nord Gascogne; Phytoplankton; Dinophysis; Gyrodinium aureolum; Hydrology; Nutrients; Coastal cruise; Northern Biscay.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00432/54323/58896.pdf
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Impact potentiel du fer présent dans les rejets urbains sur les blooms phytoplanctoniques ArchiMer
Lassus, Patrick; Le Dean, Loic.
Le traitement des effluents urbains au chlorure ferrique à la station de Toulon produit au rejet des teneurs en fer excédant largement les valeurs moyennes en eau de mer. Une incidence potentielle sur le phytoplancton d'eau rouge a été envisagée, et en particulier sur Prorocentrum minimum, espèce dont les blooms sont de plus en plus fréquents sur le littoral méditerranéen. Des sels ferreux et ferriques à degré de solubilité variable en eau de mer ont été testés sur la croissance de cet organisme. Toutes les formes expérimentées améliorent considérablement la croissance par rapport à une eau de mer carencée en fer, en particulier le citrate ferrique. L'espèce étudiée semble pouvoir être considérée comme "0pportuniste" compte tenu de sa capacité à utiliser...
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Ano: 1987 URL: http://archimer.ifremer.fr/doc/00132/24330/22329.pdf
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In search of standardisation: A comparison of toxicity bioassays on two marine crustaceans (Palaemon serratus and Tigriopus brevicornis). ArchiMer
Le Dean, Loic; Devineau, J.
In order to mitigate the insufficiency of the number of currently available toxicity tests in marine middle, two studies on the effects of cadmium and zinc were carried through with two marine crustaceans: the prawn Palaemon serratus and a copepoda Tigriopus brevicornis. The comparison of methodologies (rearing conditions - experimental procedure) and results obtained allowed to show the advantages and the disadvantages of these two tests in order to consider a standardization.
Tipo: Text Palavras-chave: Copepoda; Decapoda; Pollution tolerance; Toxicity tests; Marine crustaceans.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/publication-1780.pdf
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Influence d'un traitement au chlore sur la croissance d'une diatomée : Phaeodactylum Tricornutum (BOHLIN) ArchiMer
Bardouil, Michele; Bocquene, Gilles; Le Dean, Loic.
Ces travaux complètent l'étude de l'influence de chocs thermiques sur la croissance de la diatomée Phaeodactylum tricornutum (BOHLIN). Ils concernent les effets d'un traitement au chlore sur le développement de cette diatomée.
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Ano: 1981 URL: http://archimer.ifremer.fr/doc/00046/15749/13151.pdf
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Juin 1986 : Distribution de Dinophysis au large de la Bretagne sud ArchiMer
Lassus, Patrick; Le Dean, Loic; Bocquene, Gilles; Bardouil, Michele; Truquet, Philippe; Truquet, Isabelle; Larrazabal, M.e..
During EIGAS échointegration cruise, hydrological and phytoplanktonic sampling operations have been carried out along off shore and coastal South Brittany area, from June 8 th to 20 - 1986. Dinophysis cells densities have been evaluated at 3 depths and for 32 statlons between Penmarc'h and Belle Ile, and positive correlation with water stability index have been pointed out; high cells densities area have been detected in the South of Loire estuary
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Ano: 1986 URL: http://archimer.ifremer.fr/doc/00119/23000/20830.pdf
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Juin 1987 - Distribution de Dinophysis au large de la Bretagne sud (II) ArchiMer
Lassus, Patrick; Truquet, Philippe; Le Dean, Loic; Bardouil, Michele; Truquet, Isabelle.
As a part of hydrological sampLing operation in South Brittany, 8 transects for 26 sampling stations were made in order to determine horizontal, and vertical distribution of Dinophysis sacculus in the 10 m layer. Un like to 1986, results do not show any correlations between Dinophysis concentrations and stability index. That may, nevertheless, be due to lower cells densities in 1987 when compared to 1986. A thermal, gradient, lower than in 1986, is still observable in the Bouthern of Loire Mouth. And, as a last point, when chlorophyll a concentrations are higher in - 10 m layer than in surface layer (Audierne and Noirmoutier areas), it can be observed hight densities of an other toxie Dinoflagellate : Gyrodinium aureolum .
Tipo: Text Palavras-chave: Bretagne sud; Hydrologie; D. sacculus; G. aureolum; South Brittany; Hydrology; D. sacculus; G. aureolum.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00138/24921/23015.pdf
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Mise au point d'un test microcrustacé marin ArchiMer
Marcaillou-le Baut, Claire; Le Dean, Loic; Chiffoleau, Jean-francois; Sanjuan, Jane.
Because of the low number of currently available toxicity tests for marine environment, the authors describe an experimental procedure using a marine copepod : Tigriopus brevicornis. This method can be used to quantify the 50 % inhibition of larval production or growth. The variability in controls larval production reaches about 9 %. The toxicity of two contaminants : tributyltin (TBTF) and cadmium chloride (CdCl2) was determined with this method and the 50 % decreases in larval production are respectively 1,4 µg/l (TBTF) and 61 µg/l (Cd++).
Tipo: Text Palavras-chave: Test; Toxicité; Copépode; Étain; Cadmium; Test; Toxicity; Copepod; Tin; Cadmium.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00139/25036/23145.pdf
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Optimisation de la production en écloserie de mollusques : Mise au point d'un pilote industriel de production phytoplanctonique en continu et des techniques associées pour leur intégration en écloserie commerciale ArchiMer
Angeri, S.; Bougaran, Gael; Connan, Jean-paul; Jouin, Jean-claude; Kaas, Raymond; Le Dean, Loic; Legrand, J.; Loubiere, K.; Lukomska, Ewa; Olivo, Erell; Picquet, Marie-lise; Pruvost, J.; Robert, Rene.
Les travaux présentés dans le cadre de ce rapport d'activité rendent compte des avancées réalisées sur le plan (1) biologique, avec d'une part une étude sur le comportement du genre Chaetoceros en culture continue et, d'autre part, les résultats d'expérimentations sur les performances d'élevages larvaires réalisés sur la base de cultures de microalgues en continu, et (2) technologique, avec la continuité des actions de développement du photobioréacteur destiné aux écloseries commerciales ainsi que des périphériques indispensables à son intégration dans le secteur aquacole.
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Ano: 2007 URL: http://archimer.ifremer.fr/doc/00304/41518/40726.pdf
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Optimisation de l'enrichissement nutritif pour la culture d' Isochrysis galbana affinis Tahiti ArchiMer
Bougaran, Gael; Le Dean, Loic; Loizel, Céline; Lukomska, Ewa; Picquet, Marie-lise; Kaas, Raymond; Muller-feuga, Arnaud.
La prymnésiophycée Isochrysis galbana affinis Tahiti (T -iso) est classiquement utilisée comme nourriture de larves de mollusques et de proies vivantes de poissons d'aquaculture dans la plupart des écloseries produisant des juvéniles de ces espèces. La solution d'enrichissement de Conway (SEC), élaborée en 1966 par Walne, est largement utilisée pour la production de T -iso en batch. Cependant, les apports nutritifs de cette solution d'enrichissement ne sont pas spécifiquement optimisés pour la culture de cette algue, participant à engendrer des coûts de production élevés (Valenzuala-Espinoza et al., 1999 ; Fabregas et al., 1986) et des effluents inépuisés. Dans les 15 dernières années, plusieurs auteurs sont parvenus à réduire le coût de solutions...
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Ano: 2001 URL: http://archimer.ifremer.fr/doc/00304/41519/40727.pdf
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Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries ArchiMer
Marchetti, Julie; Bougaran, Gael; Le Dean, Loic; Megrier, Cyril; Lukomska, Ewa; Kaas, Raymond; Olivo, Erell; Baron, Regis; Robert, Rene; Cadoret, Jean-paul.
Optimal culture conditions were investigated for the continuous culture of Isochrysis affinis galbana (T-Iso) by assessing the effects of irradiance, temperature, pH and nitrogen and phosphorus requirements. Assessment of the growth rate-irradiance relationship in a turbidostat experiment highlighted that an optimum irradiance efficiency for growth (E-mu) occurred at irradiance much lower than the saturating level. Furthermore, our modeling approach for productivity pointed out that an optimal set of irradiance and dilution rate could be achieved in order to maximize irradiance efficiency. Optimal conditions for growth were assessed for temperature, pH and irradiance by means of a factorial design experiment. The resulting model predicted that optimal...
Tipo: Text Palavras-chave: Isochrysis affinis galbana; Irradiance efficiency; Factor interaction; Nutrient limitation; Microalgae cultures.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00076/18736/16465.pdf
Registros recuperados: 23
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