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Registros recuperados: 13
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Contrasting effects of historical contingency on phenotypic and genomic trajectories during a two-step evolution experiment with bacteria ArchiMer
Plucain, Jessica; Suau, Antonia; Cruveiller, Stephane; Medigue, Claudine; Schneider, Dominique; Le Gac, Mickael.
Background The impact of historical contingency, i.e. the past evolutionary history of a population, on further adaptation is mostly unknown at both the phenotypic and genomic levels. We addressed this question using a two-step evolution experiment. First, replicate populations of Escherichia coli were propagated in four different environmental conditions for 1000 generations. Then, all replicate populations were transferred and propagated for further 1000 generations to a single new environment. Results Using this two-step experimental evolution strategy, we investigated, at both the phenotypic and genomic levels, whether and how adaptation in the initial historical environments impacted evolutionary trajectories in a new environment. We showed that...
Tipo: Text Palavras-chave: Experimental evolution; Escherichia coli; Adaptation; Historical contingency; Epistasis.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00333/44447/44118.pdf
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Cophylogeny of the anther smut fungi and their caryophyllaceous hosts: Prevalence of host shifts and importance of delimiting parasite species for inferring cospeciation ArchiMer
Refregier, Guislaine; Le Gac, Mickael; Jabbour, Florian; Widmer, Alex; Shykoff, Jacqui A.; Yockteng, Roxana; Hood, Michael E.; Giraud, Tatiana.
Background: Using phylogenetic approaches, the expectation that parallel cladogenesis should occur between parasites and hosts has been validated in some studies, but most others provided evidence for frequent host shifts. Here we examine the evolutionary history of the association between Microbotryum fungi that cause anther smut disease and their Caryophyllaceous hosts. We investigated the congruence between host and parasite phylogenies, inferred cospeciation events and host shifts, and assessed whether geography or plant ecology could have facilitated the putative host shifts identified. For cophylogeny analyses on microorganisms, parasite strains isolated from different host species are generally considered to represent independent evolutionary...
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Ano: 2008 URL: http://archimer.ifremer.fr/doc/00176/28694/27138.pdf
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Étude sur la prolifération de la micro algue Alexandrium minutum en rade de Brest ArchiMer
Chapelle, Annie; Le Bec, Claude; Amzil, Zouher; Dreanno, Catherine; Guillou, Laure; Klouch, Khadidja; Labry, Claire; Le Gac, Mickael; Pineau-guillou, Lucia; Siano, Raffaele; Abernot, Chantal; Andrieux, Francoise; Caradec, Florian; Destombe, Christophe; Dia, A.; Doner, Anne; Duval, Julia; Gouriou, Jeremie; Lazure, Pascal; Le Brun, Luc; Le Gal, Dominique; Malestroit, Pascale; Petton, Sebastien; Plus, Martin; Quere, Julien; Savar, Veronique; Schmitt, Sophie; Terre, A.; Youenou, Agnes.
Suite à l’événement de grande ampleur d’efflorescence d’Alexandrium minutum et des toxicités associées en Rade de Brest en 2012, un projet a été élaboré avec la Région Bretagne dans le cadre du développement du port de Brest. L’Ifremer a organisé un échantillonnage dans l’estuaire de la rivière de Daoulas, zone la plus touchée en 2012, au cours des printemps-été 2013 et 2014. Un suivi de la présence d’A. minutum dans les sédiments a également été réalisé en hiver 2014. De ces suivis, des mesures réalisées au laboratoire à ce jour, des simulations du modèle Prévimer ainsi que des résultats du réseau Rephy et Velyger, il ressort que: * La rade de Brest peut aujourd’hui être considérée comme une zone à risque pour Alexandrium minutum. En effet, depuis 2012...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00254/36487/35029.pdf
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Etude sur la prolifération de la micro algue Alexandrium minutum en rade de Brest. Projet Daoulex. Rapport d’avancement n° 1 : Novembre 2013 ArchiMer
Chapelle, Annie; Le Bec, Claude; Le Gac, Mickael; Labry, Claire; Amzil, Zouher; Guillou, Laure; Dreanno, Catherine; Pineau-guillou, Lucia.
Suite à l’événement de grande ampleur d’efflorescence d’Alexandrium minutum et des toxicités associées en Rade de Brest en 2012, un projet a été monté avec la Région dans le cadre du développement du port de Brest. De juillet à août 2013, l’Ifremer a organisé un échantillonnage serré dans l’estuaire de Daoulas, zone la plus touchée en 2012. De ce suivi et des mesures réalisées au laboratoire à ce jour, ainsi que des résultats du réseau Rephy et Velyger, il ressort que : * Alexandrium minutum s’est développé à nouveau en rade de Brest en 2013 mais à des teneurs inférieures à 2012. * l’épisode de bloom et de toxicité supérieur aux seuils légaux s’est déroulé plus tard qu’en 2012, du 1er juillet jusqu’à 5 août. * les efflorescences et la toxicité ont touché...
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Ano: 2013 URL: https://archimer.ifremer.fr/doc/00191/30230/28644.pdf
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Evolutionary history and genetic parallelism affect correlated responses to evolution ArchiMer
Le Gac, Mickael; Cooper, Tim F.; Cruveiller, Stephane; Edigue, Claudine M.; Schneider, Dominique.
We investigated the relationship between genomic and phenotypic evolution among replicate populations of Escherichia coli evolved for 1000 generations in four different environments. By resequencing evolved genomes, we identified parallel changes in genes encoding transcription regulators within and between environments. Depending on both the environment and the altered gene, genetic parallelism at the gene level involved mutations that affected identical codons, protein domains or were widely distributed across the gene. Evolved clones were characterized by parallel phenotypic changes in their respective evolution environments but also in the three alternative environments. Phenotypic parallelism was high for clones that evolved in the same environment,...
Tipo: Text Palavras-chave: Adaptation; Epistasis; Experimental evolution; Genome sequencing; Parallelism.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00148/25928/24096.pdf
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Evolutionary processes and cellular functions underlying divergence in Alexandrium minutum ArchiMer
Le Gac, Mickael; Metegnier, Gabriel; Chomerat, Nicolas; Malestroit, Pascale; Quere, Julien; Bouchez, Olivier; Siano, Raffaele; Destombe, Christophe; Guillou, Laure; Chapelle, Annie.
Understanding divergence in the highly dispersive and seemingly homogeneous pelagic environment for organisms living as free drifters in the water column remains a challenge. Here, we analyzed the transcriptome wide mRNA sequences, as well as the morphology of 18 strains of Alexandrium minutum, a dinoflagellate responsible for Harmful Algal Blooms worldwide, to investigate the functional bases of a divergence event. Analysis of the joint site frequency spectrum (JSFS) pointed toward an ancestral divergence in complete isolations followed by a secondary contact resulting in gene flow between the two diverging groups, but heterogeneous across sites. The sites displaying fixed SNPs were associated with a highly restricted gene flow and a strong...
Tipo: Text Palavras-chave: Dinoflagellates; Harmful algal blooms; Populations Genomics; Pseudocryptic species; Speciation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45857/45529.pdf
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Historical records from dated sediment cores reveal the multidecadal dynamic of the toxic dinoflagellate Alexandrium minutum in the Bay of Brest (France) ArchiMer
Klouch, Khadidja; Schmidt, Sabine; Andrieux-loyer, Francoise; Le Gac, Mickael; Hervio-heath, Dominique; Qui-minet, Zujaila Nohemi; Quere, Julien; Bigeard, Estelle; Guillou, Laure; Siano, Raffaele.
The multiannual dynamic of the cyst-forming and toxic marine dinoflagellate Alexandrium minutum was studied over a time scale of about 150 years by a paleoecological approach based on ancient DNA (aDNA) quantification and cyst revivification data obtained from two dated sediment cores of the Bay of Brest (Brittany, France). The first genetic traces of the species presence in the study area dated back to 1873 ± 6. Specific aDNA could be quantified by a newly-developed real-time PCR assay in the upper core layers, in which the germination of the species (in up to 17–19 year-old sediments) was also obtained. In both cores studied, our quantitative paleogenetic data showed a statistically significant increasing trend in the abundance of A. minutum ITS1 rDNA...
Tipo: Text Palavras-chave: Paleoecology; Ancient DNA; Real-time PCR; Harmful algal blooms (HAB); Dinoflagellates; Coastal ecology.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00334/44516/44212.pdf
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Influence of gametogenesis pattern and sex on paralytic shellfish toxin levels in triploid Pacific oyster Crassostrea gigas exposed to a natural bloom of Alexandrium minutum ArchiMer
Hermabessiere, Ludovic; Fabioux, C.; Lassudrie, Malwenn; Boullot, F.; Long, Marc; Lambert, Christophe; Le Goic, N.; Gouriou, Jeremie; Le Gac, Mickael; Chapelle, Annie; Soudant, P.; Hegaret, Helene.
This study investigated the effect of gametogenesis pattern and sex on levels of paralytic shellfish toxins (PST) accumulated by triploid oysters Crassostrea gigas exposed to a natural bloom of the toxic dinoflagellate Alexandrium minutum in the Bay of Brest (Western Brittany, France), over the summer 2014. Toxin accumulation in oysters was proposed to be influenced by the proportion of energy allocated to reproduction versus other metabolisms, as proposed by Haberkorn et al. (2010). Thus, we hypothesized that triploid oysters with different gametogenesis patterns (α or β, producing respectively numerous gametes or rare gametes) could result in differences in toxin accumulation. Toxin level could also be different according to the gender of the oysters. To...
Tipo: Text Palavras-chave: Crassostrea gigas; Alexandrium minutum; Harmful algal bloom (HAB); Paralytic shellfish toxin (PST); Gametogenesis pattern; Triploid.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00307/41801/41010.pdf
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Inter and intra-specific growth and domoic acid production in relation to nutrient ratios and concentrations in Pseudo-nitzschia : phosphate an important factor ArchiMer
Lema, Anais Kimberley; Latimier, Marie; Nezan, Elisabeth; Fauchot, Juliette; Le Gac, Mickael.
The factors responsible for inducing the synthesis of toxins and responses from toxic phytoplankton blooms remain unclear. In this study we compare the influence of genotypic (at both the intra and interspecific levels) and environmental factors (nutrient concentration and ratio) on growth (in terms of cell densities) and domoic acid (DA) production in three Pseudo-nitzschia species: P. australis, P.pungens and P.fradulenta. A strong phosphate effect was detected. More precisely, a low initial concentration in phosphate, even at high initial nitrogen and silicate concentrations, induced the highest DA concentrations and the lowest cell densities in all strains/species studied. In contrast, a low initial concentration of nitrogen and silicate combined, with...
Tipo: Text Palavras-chave: Pseudo-nitzschia strains; Domoic acid; Ratio; Phosphate; Nitrogen.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00377/48849/49397.pdf
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Inter and intra-specific transcriptional and phenotypic responses of Pseudo-nitzschia under different nutrient conditions ArchiMer
Lema, Anais Kimberley; Metegnier, Gabriel; Quéré, Julien; Latimier, Marie; Youenou, Agnes; Lambert, Christophe; Fauchot, Juliette; Le Gac, Mickael.
Untangling the functional basis of divergence between closely related species is a step towards understanding species dynamics within communities at both the evolutionary and ecological scales. We investigated cellular (i.e: growth, domoic acid production, nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatom genus Pseudo-nitzschia. Three main results were obtained. First, strains from the same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intra-specific diversity to investigate functional divergence between species. Second, a...
Tipo: Text Palavras-chave: Phenotype; Transcriptomics; Pseudo-nitzschia; Divergence; Nutrients.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00482/59392/62157.pdf
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Species specific gene expression dynamics during harmful algal blooms ArchiMer
Metegnier, Gabriel; Paulino, Sauvann; Ramond, Pierre; Siano, Raffaele; Sourisseau, Marc; Destombe, Christophe; Le Gac, Mickael.
Harmful algal blooms are caused by specific members of microbial communities. Understanding the dynamics of these events requires comparing the strategies developed by the problematic species to cope with environmental fluctuations to the ones developed by the other members of the community. During three consecutive years, the meta-transcriptome of micro-eukaryote communities was sequenced during blooms of the toxic dinoflagellate Alexandrium minutum. The dataset was analyzed to investigate species specific gene expression dynamics. Major shifts in gene expression were explained by the succession of different species within the community. Although expression patterns were strongly correlated with fluctuation of the abiotic environment, and more...
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00622/73390/72602.pdf
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The Bay of Brest (France), a new risky site for toxic Alexandrium minutum blooms and PSP shellfish contamination ArchiMer
Chapelle, Annie; Le Gac, Mickael; Labry, Claire; Siano, Raffaele; Quere, Julien; Caradec, Florian; Le Bec, Claude; Nezan, Elisabeth; Doner, Anne; Gouriou, Jeremie.
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00278/38921/37461.pdf
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Uptake of Inorganic and Organic Nitrogen Sources by Dinophysis acuminata and D. acuta ArchiMer
García-portela, Maria; Reguera, Beatriz; Gago, Jesus; Le Gac, Mickael; Rodríguez, Francisco.
Dinoflagellate species of Dinophysis are obligate mixotrophs that require light, nutrients, and prey for sustained growth. Information about their nitrogenous nutrient preferences and their uptake kinetics are scarce. This study aimed to determine the preferred nitrogen sources in cultures of D. acuminata and D. acuta strains from the Galician Rías Baixas (NW Spain) and to compare their uptake kinetics. Well-fed versus starved cultures of D. acuminata and D. acuta were supplied with N15 labeled inorganic (nitrate, ammonium) and organic (urea) nutrients. Both species showed a preference for ammonium and urea whereas uptake of nitrate was negligible. Uptake rates by well-fed cells of D. acuminata and D. acuta were 200% and 50% higher, respectively, than by...
Tipo: Text Palavras-chave: Dinophysis; Nitrate; Ammonium; Urea; Uptake rates; N-15 incubations; Antibiotic treatment.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71770/70250.pdf
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