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Registros recuperados: 87
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A scientific review of binding and inactivation of viruses on and in food, with a focus on the role of the matrix ArchiMer
Le Guyader, Soizick; Atmar, Robert.
Tipo: Text
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00066/17762/15276.pdf
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Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers ArchiMer
Hubert, Francoise; Morga, Benjamin; Renault, Tristan; Le Guyader, Soizick.
Aims This study was performed to develop passive sampling methodology for the detection of two viruses in seawater in the area of shellfish production, The Norovirus (NoV), a human pathogen implicated in gastroenteritis outbreaks linked to oyster consumption and the ostreid herpesvirus type 1 (OsHV-1) a virus associated with mass mortalities of Pacific oysters. Methods and Results Commercially membranes were tested for their capacity to adsorb virus: Zetapor, gauze, nylon, low density polyethylene (LDPE) and Polyvinylidene difluoride (PVDF). Laboratory exposures of membranes to contaminated water samples (stool, sewage, seawater) were performed. Our data shown that the amount of NoV GII genome per membrane measured with qRT-PCR increased with the time of...
Tipo: Text Palavras-chave: Norovirus; OsHV-1; Pacific oysters; Passive samplers; Seawater; Sewage.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00365/47646/47670.pdf
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Aichi Virus, Norovirus, Astrovirus, Enterovirus, and Rotavirus Involved in Clinical Cases from a French Oyster-Related Gastroenteritis Outbreak ArchiMer
Le Guyader, Soizick; Le Saux, Jean-claude; Ambert-balay, Katia; Krol, Joanna; Serais, Ophelie; Parnaudeau, Sylvain; Giraudon, Helene; Delmas, Gilles; Pommepuy, Monique; Pothier, Pierre; Atmar, Robert L..
Following a flooding event close to a shellfish production lagoon, 205 cases of gastroenteritis were linked to oyster consumption. Twelve stool samples from different individuals were collected. Analysis showed that eight samples were positive for multiple enteric viruses, and one stool sample had seven different enteric viruses. Analysis of shellfish implicated in the outbreak allowed detection of the same diversity of enteric viruses, with some viral genomic sequences being identical to those obtained from stool sample analysis. Shellfish were contaminated by as many as five different enteric viruses. For the first time in Europe, Aichi virus was identified in oyster samples. Shellfish samples collected over 3 weeks following the outbreak showed a...
Tipo: Text
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6099.pdf
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An oyster-associated hepatitis A outbreak in France in 2007 ArchiMer
Guillois-becel, Y.; Couturier, E.; Le Saux, Jean-claude; Roque-afonso, A. M.; Le Guyader, Soizick; Le Goas, A.; Pernes, J.; Le Bechec, S.; Briand, A.; Robert, C.; Dussaix, E.; Pommepuy, Monique; Vaillant, V..
Following the notification of nine hepatitis A cases clustered in the Cotes d'Armor district in northwestern France, epidemiological, environmental and microbiological investigations were set up in order to identify the source and vehicle of contamination and implement control measures. In total, 111 cases were identified in the outbreak, all of whom lived or had stayed as tourists in the Cotes d'Armor district. Of the cases, 87% had eaten raw shellfish, and 81% specifically oysters. Traceback investigations carried out on raw shellfish consumed by the cases showed that the raw shellfish originated from a single shellfish farm. The shellfish were probably contaminated either in the submersible tanks or in a depuration land-based tank where they were...
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6685.pdf
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Analytical Methods for Virus Detection in Water and Food ArchiMer
Bosch, Albert; Sanchez, Gloria; Abbaszadegan, Morteza; Carducci, Annalaura; Guix, Susana; Le Guyader, Soizick; Netshikweta, Rembuluwani; Pinto, Rosa M.; Van Der Poel, Wim H. M.; Rutjes, Saskia; Sano, Daisuke; Taylor, Maureen B.; Van Zyl, Walda B.; Rodriguez-lazaro, David; Kovac, Katarina; Sellwood, Jane.
Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses...
Tipo: Text Palavras-chave: Enteric viruses; Gastroenteritis; Hepatitis; Detection; Concentration.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14191/11465.pdf
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Assessment of human enteric viruses in cultured and wild bivalve molluscs ArchiMer
Luz Vilarino, M.; Le Guyader, Soizick; Polo, David; Schaeffer, Julien; Krol, Joanna; Romalde, Jesus L..
Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ria de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from Cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated...
Tipo: Text Palavras-chave: Seafood industry; Viral quantification; Viral prevalence; Norovirus; Hepatitis A virus; Enteric viruses; Molluscs.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6957.pdf
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Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France ArchiMer
Grodzki, Marco; Schaeffer, Julien; Piquet, Jean-come; Le Saux, Jean-claude; Cheve, Julien; Ollivier, Joanna; Le Pendu, Jacques; Le Guyader, Soizick.
Hepatitis E virus (HEV), an enteric pathogen of both humans and animals, is excreted by infected individuals and is therefore present in wastewaters and coastal waters. As bivalve molluscan shellfish are known to concentrate viral particles during the process of filter feeding, they may accumulate this virus. The bioaccumulation efficiencies of oysters (Crassostrea gigas), flat oysters (Ostrea edulis), mussels (Mytilus edulis), and clams (Ruditapes philippinarum) were compared at different time points during the year. Tissue distribution analysis showed that most of the viruses were concentrated in the digestive tissues of the four species. Mussels and clams were found to be more sensitive to sporadic contamination events, as demonstrated by rapid...
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00204/31501/29905.pdf
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Calicivirus Removal in a Membrane Bioreactor Wastewater Treatment Plant ArchiMer
Sima, Laura C.; Schaeffer, Julien; Le Saux, Jean-claude; Parnaudeau, Sylvain; Elimelech, Menachem; Le Guyader, Soizick.
To evaluate membrane bioreactor wastewater treatment virus removal, a study was conducted in southwest France. Samples collected from plant influent, an aeration basin, membrane effluent, solid sludge, and effluent biweekly from October 2009 to June 2010 were analyzed for calicivirus (norovirus and sapovirus) by real-time reverse transcription-PCR (RT-PCR) using extraction controls to perform quantification. Adenovirus and Escherichia coli also were analyzed to compare removal efficiencies. In the influent, sapovirus was always present, while the norovirus concentration varied temporally, with the highest concentration being detected from February to May. All three human norovirus genogroups (GI, GII, and GIV) were detected in effluent, but GIV was never...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15378/12810.pdf
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Characterization of new recombinant noroviruses ArchiMer
Ambert Balay, K; Bon, F; Le Guyader, Soizick; Pothier, P; Kohli, E.
Noroviruses are important etiologic agents of acute gastroenteritis and show great genetic diversity. To characterize more fully previously detected strains that could not be assigned unequivocally to one particular genotype based on the RNA polymerase, we have sequenced a region in the capsid gene and, in some cases, in the junction between open reading frame 1 (ORF1) and ORF2. The results allowed us to identify several recombinant noroviruses: GGHb viruses were detected for the first time in France in August 2000 and then spread through France and to Europe during the following winter. Here we present the characterization of three other probable GII recombinants which showed different phylogenetic positions depending on their ORF1 and ORF2 sequences....
Tipo: Text Palavras-chave: Genogroup; RNA recombination; Gastroenteritis; Round structured virus; Reverse transcription; Norovirus.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1234.pdf
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Characterization of Norovirus and Other Human Enteric Viruses in Sewage and Stool Samples Through Next-Generation Sequencing ArchiMer
Strubbia, Sofia; Phan, My V. T.; Schaeffer, Julien; Koopmans, Marion; Cotten, Matthew; Le Guyader, Soizick.
This study aimed to optimize a method to identify human enteric viruses in sewage and stool samples using random primed next-generation sequencing. We tested three methods, two employed virus enrichment based on the binding properties of the viral capsid using pig-mucin capture or by selecting viral RNA prior to library preparation through a capture using the SureSelect target enrichment. The third method was based on a non-specific biophysical precipitation with polyethylene glycol. Full genomes of a number of common human enteric viruses including norovirus, rotavirus, husavirus, enterovirus and astrovirus were obtained. In stool samples full norovirus genome were detected as well as partial enterovirus genome. A variety of norovirus sequences was...
Tipo: Text Palavras-chave: Human enteric viruses; Norovirus; Sewage; Metagenomic; Virome.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00512/62332/66592.pdf
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Complete Genome Sequence of Campylobacter armoricus CA639, Which Carries Two Plasmids, Compiled Using Oxford Nanopore and Illumina Sequencing Technologies ArchiMer
Boukerb, Amine Mohamed; Schaeffer, Julien; Serghine, Joelle; Carrier, Gregory; Le Guyader, Soizick; Gourmelon, Michele.
As determined by a hybrid approach combining Oxford Nanopore MinION and Illumina MiniSeq sequence data, Campylobacter armoricus strain CA639 harbored a circular chromosome of 1,688,169 bp with a G+C content of 28.47% and two plasmids named pCA639-1 and pCA639-2, with lengths of 51,123 and 28,139 bp, and G+C contents of 26.5% and 28.45%, respectively.
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00600/71164/69495.pdf
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Comprehensive Analysis of a Norovirus-Associated Gastroenteritis Outbreak, from the Environment to the Consumer ArchiMer
Le Guyader, Soizick; Krol, Joanna; Ambert-balay, Katia; Ruvoen-clouet, Nathalie; Desaubliaux, Benedicte; Parnaudeau, Sylvain; Le Saux, Jean-claude; Ponge, Agnes; Pothier, Pierre; Atmar, Robert L.; Le Pendu, Jacques.
Noroviruses have been recognized to be the predominant agents of nonbacterial gastroenteritis outbreaks in humans, and their transmission via contaminated shellfish consumption has been demonstrated. Norovirus laboratory experiments, volunteer challenge studies, and community gastroenteritis outbreak investigations have identified human genetic susceptibility factors related to histo-blood group antigen expression. Following a banquet in Brittany, France, in February 2008, gastroenteritis cases were linked to oyster consumption. This study identified an association of the norovirus illnesses with histo-blood group expression, and oyster contamination with norovirus was confirmed by qualitative and quantitative analyses. The secretor phenotype was...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11309/8050.pdf
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Contamination des coquillages par les norovirus en rivière d'Auray en lien avec la rénovation d'une station d'épuration littorale ArchiMer
Treguier, Cathy; Schaeffer, Julien; Le Saux, Jean-claude; Le Guyader, Soizick; Cochennec-laureau, Nathalie.
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00312/42358/41693.pdf
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Contamination des coquillages par les norovirus en rivière d'Auray (Norocoqauray) ArchiMer
Treguier, Cathy; Schaeffer, Julien; Le Saux, Jean-claude; Cochennec-laureau, Nathalie; Le Guyader, Soizick.
Les norovirus (NoV) sont responsables des épidémies hivernales de gastroentérites aiguës dans la population. Ils sont excrétés dans les fèces humains, puis se retrouvent, après épuration insuffisante, dans le milieu marin naturel où ils peuvent contaminer des coquillages, qui à leur tour risquent de déclencher des gastroentérites chez les consommateurs. Cette étude vise à améliorer les connaissances sur la contamination des coquillages en élevage en rivière d’Auray par les NoV, en lien avec les bactéries témoins de contamination fécale Escherichia coli (E. coli). L’objectif est de suivre les flux de NoV et d’E. coli apportés par différents rejets (dont une station d’épuration) impactant la zone conchylicole et leur influence sur la qualité des coquillages...
Tipo: Text Palavras-chave: Norovirus; Coquillages; Contamination; Station d’épuration; Flux; Rivière d’Auray; Escherichia coli..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00267/37778/35829.pdf
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Detecting virus contamination in shellfish ArchiMer
Bosch, Albert; Lees, D.; Von Bonsdorff, C.h.; Pinto, R.m.; Crocci, L.; De Medici, D.; Le Guyader, Soizick.
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Ano: 2008 URL: http://archimer.ifremer.fr/doc/00066/17767/15283.pdf
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Detection and Quantification of Noroviruses in Shellfish ArchiMer
Le Guyader, Soizick; Parnaudeau, Sylvain; Schaeffer, Julien; Bosch, Albert; Loisy, Fabienne; Pommepuy, Monique; Atmar, Robert L..
Noroviruses (NoVs) are the most common viral agents of acute gastroenteritis in humans, and high concentrations of NoVs are discharged into the environment. As these viruses are very resistant to inactivation, the sanitary consequences are contamination of food, including molluscan shellfish. There are four major problems with NoV detection in shellfish samples: low levels of virus contamination, the difficulty of efficient virus extraction, the presence of interfering substances that inhibit molecular detection, and NoV genetic variability. The aims of this study were to adapt a kit for use with a method previously shown to be efficient for detection of NoV in shellfish and to use a one step real-time reverse transcription-PCR method with addition of an...
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6137.pdf
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Détection des virus entériques dans les stations d'épuration par PCR ArchiMer
Le Guyader, Soizick; Dubois, Eric; Menard, Dominique.
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Ano: 1994 URL: http://archimer.ifremer.fr/doc/00041/15209/12537.pdf
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Detection of Hepatitis E Virus in Sewage After an Outbreak on a French Island ArchiMer
Miura, Takayuki; Lhomme, Sebastien; Le Saux, Jean-claude; Le Mehaute, Philippe; Guillois, Yvonnick; Couturier, Elizabeth; Izopet, Jacques; Abranavel, Florence; Le Guyader, Soizick.
A hepatitis E outbreak, which occurred on a small isolated island, provided an opportunity to evaluate the association between the number of hepatitis E cases in the community and the concentration of virus detected in sewage. Samples were collected from the different sewage treatment plants from the island and analyzed for the presence of hepatitis E (HEV) virus using real-time RT-PCR. We demonstrated that if 1–4 % of inhabitants connected to a WWTP were infected with HEV, raw sewage contained HEV at detectable levels. The finding that such a small number of infected people can contaminate municipal sewage works raises the potential of the further distribution of the virus. Indeed, investigating the routes of transmission of HEV, including the potential...
Tipo: Text Palavras-chave: Hepatitis E virus; Wastewater treatment plant; Quantification.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00334/44482/44199.pdf
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Detection of human enteric viruses in shellfish collected in Tunisia ArchiMer
Elamri, D; Aouni, M; Parnaudeau, Sylvain; Le Guyader, Soizick.
Aims: The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. Methods and Results: Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found...
Tipo: Text Palavras-chave: Shellfish; Reverse transcription PCR; Human enteric viruses.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1881.pdf
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Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption ArchiMer
Le Guyader, Soizick; Bon, Fabienne; Demedici, Dario; Parnaudeau, Sylvain; Bertone, Alessandra; Crudeli, Silvia; Doyle, Aoife; Zidane, Mohamed; Suffredini, Elisabetta; Kohli E, Evelyne; Maddalo, Francesco; Monini, Marina; Gallay, Anne; Pommepuy, Monique; Pothier, Pierre; Ruggeri, Franco M..
An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to...
Tipo: Text Palavras-chave: Seafood; Contamination; Norovirus; Epidemiology; Virology; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2096.pdf
Registros recuperados: 87
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