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Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos ArchiMer
Sussarellu, Rossana; Lebreton, Morgane; Rouxel, Julien; Akcha, Farida; Riviere, Guillaume.
Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development. For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval...
Tipo: Text Palavras-chave: Oyster; Embryotoxicity; Copper; Gene expression; DNA methylation.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00417/52856/53756.pdf
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