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Registros recuperados: 5
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A physical map of the heterozygous grapevine 'Cabernet Sauvignon' allows mapping candidate genes for disease resistance Inra
Moroldo, M.; Paillard, S.; Marconi, R.; Legeai, F.; Canaguier, A.; Cruaud, C.; de Berardinis, V.; Guichard, C.; Brunaud, V.; Le Clainche, I.; Scalabrin, S.; Testolin, R.; di Gaspero, G.; Morgante, M.; Adam-Blondon, A.F..
Background: Whole-genome physical maps facilitate genome sequencing, sequence assembly, mapping of candidate genes, and the design of targeted genetic markers. An automated protocol was used to construct a Vitis vinifera 'Cabernet Sauvignon' physical map. The quality of the result was addressed with regard to the effect of high heterozygosity on the accuracy of contig assembly. Its usefulness for the genome-wide mapping of genes for disease resistance, which is an important trait for grapevine, was then assessed. Results: The physical map included 29,727 BAC clones assembled into 1,770 contigs, spanning 715,684 kbp, and corresponding to 1.5-fold the genome size. Map inflation was due to high heterozygosity, which caused either the separation of allelic...
Tipo: Journal Article Palavras-chave: MILDIOU; MALADIE FONGIQUE; CARTE GENETIQUE; SYSTEME IMMUNITAIRE; VIGNE; RESISTANCE AUX MALADIES; CARTOGRAPHIE; GENE CANDIDAT; GENETIQUE; SEQUENCE NUCLEOTIDIQUE; CABERNET SAUVIGNON PLANT IMMUNE SYSTEM; VITIS VINIFERA L.; NONHOST RESISTANCE; POWDERY MILDEW; GENOME SEQUENCE; LINKAGE MAP; RICE GENOME; BAC; ARABIDOPSIS; GENETICS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011dc1cb8fa&uri=/notices/prodinra1/2011/03/
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An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research Inra
Legeai, F.; Malpel, S.; Montagné, N.; Monsempes, C.; Cousserans, F.; Merlin, C.; Francois, M.C.; Maibeche Coisne, M.; Gavory, F.; Poulain, J.; Jacquin Joly, E..
Background :Nocturnal insects such as moths are ideal models to study the molecular bases of olfaction that they use, among examples, for the detection of mating partners and host plants. Knowing how an odour generates a neuronal signal in insect antennae is crucial for understanding the physiological bases of olfaction, and also could lead to the identification of original targets for the development of olfactory-based control strategies against herbivorous moth pests. Here, we describe an Expressed Sequence Tag (EST) project to characterize the antennal transcriptome of the noctuid pest model, Spodoptera littoralis, and to identify candidate genes involved in odour/pheromone detection.Results :By targeting cDNAs from male antennae, we biased gene...
Tipo: Journal Article Palavras-chave: DETECTION OLFACTIVE; PAPILLON DE NUIT; SEQUENCE GENOMIQUE; NOCTUELLE NOCTUID MOTH SPODOPTERA LITTORALIS; PHEROMONE; OLFACTORY DETECTION.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201159b3cef&uri=/notices/prodinra1/2011/05/
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Analysis of virion structural components reveals vestiges of the ancestral ichnovirus genome Inra
Volkoff, A.N.; Jouan, V.; Urbach, S.; Samain, S.; Bergoin, M.; Wincker, P.; Demettre, E.; Cousserans, F.; Provost, B.; Coulibaly, F.; Legeai, F.; Béliveau, C.; Cusson, M.; Gyapay, G.; Drezin, J.M..
Many thousands of endoparasitic wasp species are known to inject polydnavirus (PDV) particles into their caterpillar host during oviposition, causing immune and developmental dysfunctions that benefit the wasp larva. PDVs associated with braconid and ichneumonid wasps, bracoviruses and ichnoviruses respectively, both deliver multiple circular dsDNA molecules to the caterpillar. These molecules contain virulence genes but lack core genes typically involved in particle production. This is not completely unexpected given that no PDV replication takes place in the caterpillar. Particle production is confined to the wasp ovary where viral DNAs are generated from proviral copies maintained within the wasp genome. We recently showed that the genes involved in...
Tipo: Journal Article Palavras-chave: POLYDNAVIRUS; ENDOPARASITOIDE LARVAIRE; NOCTUELLE; HYPOSOTER DIDYMATOR; VIRUS SYMBIOTIQUE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20115e58bd94&uri=/notices/prodinra1/2011/02/
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Extensive synteny conservation of holocentric chromosomes in Lepidoptera despite high rates of local genome rearrangements Inra
D'Alençon, E.; Sezutsu, H.; Legeai, F.; Permal, E.; Bernard-Samain, S.; Gimenez, S.; Gagneur, C.; Cousserans, F.; Shimomura, M.; Brun-Barale, A.; Flutre, T.; Couloux, A.; East, P.; Mita, K.; Quesneville, H.; Fournier, P.; Feyereisen, R..
The recent assembly of the silkworm Bombyx mori genome with 432 Mb on 28 holocentric chromosomes has become a reference in the genomic analysis of the very diverse Order of Lepidoptera. We sequenced BACs from two major pests, the noctuid moths Helicoverpa armigera and Spodoptera frugiperda, corresponding to 15 regions distributed on 11 B. mori chromosomes, each BAC/region being anchored by known orthologous gene(s) to analyze syntenic relationships and genome rearrangements among the three species. Nearly 300 genes and numerous transposable elements were identified, with long interspersed nuclear elements and terminal inverted repeats the most abundant transposable element classes. There was a high degree of synteny conservation between B. mori and the two...
Tipo: Journal Article Palavras-chave: COMPARATIVE GENOMICS; SILKWORM; NOCTUIDAE; TRANSPOSABLE ELEMENTS; GENE CLUSTERS.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011a868a119&uri=/notices/prodinra1/2011/02/
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Strong heterogeneity in nucleotidic composition and codon bias in the pea aphid (Acyrthosiphon pisum) shown by EST-based coding genome reconstruction Inra
Rispe, C.; Legeai, F.; Gauthier, J.P.; Tagu, D..
The aim of this study was to analyze patterns of nucleotidic composition and codon usage in the pea aphid genome (Acyrthosiphon pisum). A collection of 60,000 expressed sequence tags (ESTs) in the pea aphid has been used to automatically reconstruct 5809 coding sequences (CDSs), based on similarity with known proteins and on coding style recognition. Reconstructions were manually checked for ribosomal proteins, leading to tentatively reconstruct the nea-complete set of this category. Pea aphid coding sequences showed a shift toward AT (especially at the third codon position) compared to drosophila homologues. Genes with a putative high level of expression (ribosomal and other genes with high EST support) remained more GC3-rich and had a distinct codon...
Tipo: Journal Article Palavras-chave: CODON; PUCERON; ACYRTHOSIPHON PISUM; SEQUENCE EST.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20081541cd5d&uri=/notices/prodinra1/2010/11/
Registros recuperados: 5
Primeira ... 1 ... Última
 

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