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Registros recuperados: 27
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A "G" chromosome banding study of three cupped oyster species : Crassostrea gigas, Crassostrea angulata and Crassostrea virginica (Mollusca : Bivalvia) ArchiMer
Leitao, Alexandra; Thiriot-quievreux, Catherine; Malheiro, Isabel; Boudry, Pierre.
The G-banding technique was performed on chromosomes from gill tissue of three cupped oyster species : Crassostrea gigas, Crassostrea angulata and Crassostrea virginica. Identification of the ten individual chromosome pairs was obtained. Comparative analysis of G-banded karyotypes of the three species showed that their banding patterns generally resembled each other, with chromosome pair 3 being similar in all three species. However, differences from one species to another were also observed. The G-banding pattern highlighted greater similarities between C. gigas and C. angulata than between these two species and C. virginica, thus providing an additional argument for genetic divergence between, these two evolutionary lineages. C. gigas and C. angulata...
Tipo: Text Palavras-chave: Crassostrea virginica; Crassostrea angulata; Crassostrea gigas; G banding; Chromosome.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-2736.pdf
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A Microarray-Based Analysis of Gametogenesis in Two Portuguese Populations of the European Clam Ruditapes decussatus ArchiMer
Sousa, Joana; Milan, Massimo; Bargelloni, Luca; Pauletto, Marianna; Matias, Domitilia; Joaquim, Sandra; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud.
The European clam, Ruditapes decussatus is a species with a high commercial importance in Portugal and other Southern European countries. Its production is almost exclusively based on natural recruitment, which is subject to high annual fluctuations. Increased knowledge of the natural reproductive cycle of R. decussatus and its molecular mechanisms would be particularly important in providing new highly valuable genomic information for better understanding the regulation of reproduction in this economically important aquaculture species. In this study, the transcriptomic bases of R. decussatus reproduction have been analysed using a custom oligonucleotide microarray representing 51,678 assembled contigs. Microarray analyses were performed in four gonadal...
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29935/28623.pdf
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A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus ArchiMer
De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud.
A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment...
Tipo: Text Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00260/37147/35676.pdf
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Aneuploidy and its relationship with growth in different populations of the Pacific oyster (Crassostrea gigas, Thunberg) ArchiMer
Boudry, Pierre; Leitao, Alexandra; Mccombie, Helen; Thiriot-quievreux, Catherine.
Cytogenetic abnormalities arising both in mitosis and meiosis are known to be common in bivalves. Here we review result obtained from the observations of somatic aneuploidy in different populations of the commercially important Pacific oyster, Crassostrea gigas. The oysters studied were either (1) produced in IFREMER hatcheries between 1986 and 1999, (some of these were part of the EU funded programme GENEPHYS); or (2) collected from wild populations along the French Atlantic coast. Chromosome numbers were scored from 30 mitotic metaphases in gill tissue per individual studied. Aneuploid cells (2n - X) were observed in all the 13 populations studied. A highly significant negative correlation was observed between the level of aneuploidy and growth, i.e....
Tipo: Text Palavras-chave: Growth; Genetic; Aneuploidy; Crassostrea gigas; Pacific oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3446.pdf
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Comparative karyological study of cupped oyster species ArchiMer
Leitao, Alexandra; Boudry, Pierre; Labat, Jean-philippe; Thiriot-quievreux, Catherine.
Chromosomes of six cupped oyster species were studied using karyometric analysis alter conventional Giemsa staining, and silver staining, Karyotypes of Crassotrea angulata (nine metacentric and one submetacentric chromosome pairs), C. sikamea (nine metacentric and one submetacentric chromosome pairs), C. virginica (eight metacentric and two submetacentric chromosome pairs), C, ariakensis (eight metacentric and two submetacentric chromosome pairs), C, gasar (six metacentric and four submetacentric chromosome pairs), and Saccostrea commercialis (eight metacentric and two submetacentric chromosome pairs) are distinguishable by the number and position of the submetacentric chromosome pair and by the location of nucleolus organizer regions, Comparative...
Tipo: Text Palavras-chave: Bivalvia; Cytotaxonomy; Karyotype; Chromosome; Cupped oyster.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-2737.pdf
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Cytogenetic study of Ostrea conchaphila (Mollusca : Bivalvia) and comparative karyological analysis within Ostreinae ArchiMer
Leitao, Alexandra; Chaves, Raquel; Santos, Sara; Boudry, Pierre; Guedes Pinto, Henrique; Thiriot Quievreux, Catherine.
Chromosome preparations of the Olympia oyster Ostrea conchaphila Carpenter were studied using conventional Giemsa, silver staining, and C-banding techniques. The karyotype consists of six metacentric (1, 2, 4, 6, 8, and 10) and four submetacentric (3, 5, 7, 9) chromosome pairs. The silver-stained nucleolus organizer regions (Ag-NORs) were terminally located on the short arms of the submetacentric pair 5 (56% of cases) and on the long arms of submetacentric pair 7 (6% of cases). Constitutive heterochromatin was observed as telomeric C-bands on the short arm of the NOR-bearing chromosome pair 5 and as centromeric blocks of several chromosome pairs. Comparative analysis of patterns of karyotype, Ag-NORs, and C-bands of this species and of five other flat...
Tipo: Text Palavras-chave: Ostreinae; Cytotaxonomy; Cytogenetics; Ostrea conchaphila.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-2792.pdf
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Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Chaves, Raquel; Guedes Pinto, Henrique; Boudry, Pierre; Lapegue, Sylvie.
Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were...
Tipo: Text Palavras-chave: Crassostrea gigas; Pacific oyster; Restriction enzyme digestion chromosome banding; Atrazine; Aneuploidy; Crassostrea gigas; Huître creuse; Marquage chromosomique par digestion d'enzymes de restriction; Atrazine; Aneuploïdie.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/2005/publication-654.pdf
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Etude des anomalies chromosomiques chez Crassostrea gigas ArchiMer
Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu'il existe, chez l'huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text Palavras-chave: Génétique; Chromosomes; Anomalies; Aneuploïdie; Atrazine; Huîtres creuses; Crassostrea gigas.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00199/31013/29417.pdf
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Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat de Plan Région Poitou-Charentes 2000-2006. Convention 2000 ArchiMer
Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00043/15474/12853.pdf
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Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat Région Poitou-Charentes 2000-2006. Convention 2002 ArchiMer
Bouilly, Karine; Mccombie, Helen; Leitao, Alexandra; Lapegue, Sylvie.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Cette corrélation négative entre l’aneuploïdie somatique et le taux de croissance a été décrite à de nombreuses reprises (Thiriot-Quiévreux et al., 1988, 1992 ; Leitão et al., 2001a ; Zouros et al., 1996). Bien que les causes...
Tipo: Text Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15475/12854.pdf
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Etude des anomalies chromosomiques chez Crassostrea gigas. Contrat Région Poitou-Charentes 2000-2006. Convention 99 ArchiMer
Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00043/15473/12852.pdf
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Etude des ressources génétiques chez les huîtres creuses ArchiMer
Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Lam, Katherine; Mccombie, Helen; Leitao, Alexandra; Gerard, Andre.
L'étude de la variabilité génétique de l'huître creuse Crassostrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 'ro de la production mondiale d'huîtres. Introduite en France au début des années 1970 pour remplacer l'huître portugaise Crassostrea angulata, c'est aujourd'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n'a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la...
Tipo: Text Palavras-chave: Génétique; Huîtres creuses; Crassostrea gigas; Crassostrea gasar; Variabilité génétique.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00199/31008/29411.pdf
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Evidence of differential chromosome loss in aneuploid karyotypes of the Pacific oyster, Crassostrea gigas ArchiMer
Leitao, Alexandra; Boudry, Pierre; Thiriot Quievreux, Catherine.
The G-banding technique was performed on aneuploid karyotypes from gill tissue of the Pacific oyster, Crassostrea gigas, to assess whether chromosome losses could be explained by differential chromosomal susceptibility and to clarify the negative correlation between aneuploidy and growth rate previously reported in different populations of this oyster. The study of 95 G-banded aneuploid karyotypes showed that only 4 of the 10 chromosome pairs (viz. 1, 5, 9, and 10) of C. gigas were affected by the loss of one homologous chromosome. Pairs 1, 9, and 10, which were lost in 56, 33, and 44% of cases, respectively, may be considered to be differentially affected. Hypotheses on this differential chromosomal susceptibility are discussed.
Tipo: Text Palavras-chave: Oyster; Karyotype; G banding; Growth; Aneuploidy; Huître; Caryotype; Bandes G; Croissance; Aneuploïdie.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-742.pdf
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Experimental evidence for a genetic basis to differences in aneuploidy in the Pacific oyster (Crassostrea gigas) ArchiMer
Leitao, Alexandra; Boudry, Pierre; Mccombie, Helen; Gerard, Andre; Thiriot-quievreux, Catherine.
Aneuploidy has been previously reported in wild and cultivated Pacific oysters, Crassostrea gigas, and has been shown to be negatively correlated with growth. This is especially important since high variability of growth rate is one of the major problems in the aquacultural production of this species. The existence of a genetic basis for the observed differences in aneuploidy was first investigated through the comparative study of six full-sib families, with mean individual weights ranging from 0.59 to 1.49 g. The slowest growing family was found to have the highest level of aneuploidy. Significant differences in aneuploidy were also found among families when individuals with the same growth rate were sampled. This supports the hypothesis of the existence...
Tipo: Text Palavras-chave: Oyster; Growth; Genetics; Full sib families; Crassostrea gigas; Aneuploidy; Huître; Croissance; Génétique; Famille de plein frères; Crassostrea gigas; Aneuploïdie.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-2700.pdf
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Impact of atrazine on somatic aneuploidy in cupped oysters, Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Chaves, R.; Guedes Pinto, H; Boudry, Pierre; Lapegue, Sylvie.
In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species
Tipo: Text Palavras-chave: Genetic; Aneuploidy; Toxicology; Impact; Atrazine; Crassostrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3489.pdf
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Impact of pollutants on aneuploidy in the Pacific oyster, Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Miramand, Pierre; Lapegue, Sylvie.
Aneuploidy is the alteration of the normal diploid chromosome number. In the Pacifie oyster, Crassastrea gigas, hypodiploid cells have regularly been reported as have a negative correlation between this phenomenon and growth and evidence for a genetic basis. We previously demonstrated a positive relationship between a pollutant, atrazine, and aneuploidy in Crassastrea gigas adults and juveniles. To evaluate the persistence of this impact, the present study focused on a sample of the same juveniles previously exposed to different atrazine treatments (0.0 1 mg/l which represents a peak value found in a polluted environment and 0.1 mg/l) for three and a half months. Then, we evaluated them for aneuploidy after another two and a half months in non polluted...
Tipo: Text Palavras-chave: Cadmium; Atrazine; Pollutant; Pacific oyster; Aneuploidy.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2852.pdf
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Insights into Molecular Features of Venerupis decussata Oocytes: A Microarray-Based Study ArchiMer
Pauletto, Marianna; Milan, Massimo; Teixeira De Sousa, Joana; Huvet, Arnaud; Joaquim, Sandra; Matias, Domitilia; Leitao, Alexandra; Patarnello, Tomaso; Bargelloni, Luca.
The production of Venerupis decussata relies on wild seed collection, which has been recently compromised due to recruitment failure and severe mortalities. To address this issue and provide an alternative source of seed, artificial spawning and larval rearing programs were developed. However, hatchery-based seed production is a relatively new industry and it is still underdeveloped. A major hurdle in the European clam seed production is the control of spawning and reproduction, which is further hindered by the impossibility of obtaining fertile gametes by gonadal "stripping'', as meiosis re-initiation is constrained to a maturation process along the genital ducts. In the present study, oocytes were collected from 15 females and microarray analyses was...
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00254/36532/35059.pdf
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Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids ArchiMer
Batista, Frederico M.; Lopez-sanmartin, Monserrat; Boudry, Pierre; Navas, Jose I.; Ruano, Francisco; Renault, Tristan; Fonseca, Vera G.; Leitao, Alexandra.
Cytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid...
Tipo: Text Palavras-chave: Aneuploidy; Ostreid herpesvirus 1; Oyster; Crassostrea.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00215/32581/35759.pdf
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Le phénomène de l'anauploïdie et sa relation avec la croissance chez l'huître creuse Crassostrea gigas ArchiMer
Leitao, Alexandra; Boudry, Pierre; Mccombie, Helen; Lapegue, Sylvie; Gerard, Andre; Thiriot-quievreux, Catherine.
Cytogenetic abnormalities have often been observed on bivalves on meiosis or mitosis. This is a review of various observations of somatic aneuploidy conducted on different populations of Crassastrea gigas.
Tipo: Text Palavras-chave: Croissance; Aneuploïdie; Huîtres; Génétique; Crassostrea gigas; Cytogénétique.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3280.pdf
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New insights about the introduction of the Portuguese oyster, Crassostrea angulata, into the North East Atlantic from Asia based on a highly polymorphic mitochondrial region ArchiMer
Grade, Ana; Chairi, Hicham; Lallias, Delphine; Power, Deborah M.; Ruano, Francisco; Leitao, Alexandra; Drago, Teresa; King, Jonathan W.; Boudry, Pierre; Batista, Frederico M..
It is commonly presumed that the Portuguese oyster Crassostrea angulata was introduced into the North East (NE) Atlantic from Asia. The analysis of the nucleotide sequence of a highly polymorphic non-coding mitochondrial region (major noncoding region - MNR) of C. angulata samples collected in Europe (Portugal), Africa (Morocco) and Asia (Shantou and Taiwan) provided new insight into the introduction of this species into the NE Atlantic. Sixty haplotypes and a nucleotide diversity of 0.0077 were observed in 130 analyzed sequences. Higher nucleotide diversity levels were observed in NE Atlantic sites than in Asian sites and significant genetic differentiation was found between the two. Our results suggest that C. angulata might have been introduced to the...
Tipo: Text Palavras-chave: Crassostrea angulata; Phylogeography; Genetic signature; Biological invasions.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00371/48200/48316.pdf
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