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| Registros recuperados: 11 | |
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| Kozlowska-Makulska, A.; Beuve, M.; Syller, J.; Szyndel, M.S.; Lemaire, O.; Bouzoubaa, S.; Herrbach, E.. |
| Different field isolates of the ‘beet poleroviruses’Beet mild yellowing virus (BMYV) and Beetchlorosis virus (BChV) (genus Polerovirus, familyLuteoviridae) collected in France and Poland wereevaluated for transmissibility from and to sugar beetplants by different aphid species. In general, bothBMYV and BChV were efficiently transmitted by Myzus persicae and by a French clone of Macrosiphumeuphorbiae. In contrast, transmissibility of the twopoleroviruses by an English clone of M. euphorbiaewas evidently weaker, although the aphid samplescontained the virus as demonstrated by RT-PCR. Noneof the BMYV or BChV isolates was transmitted byAphis fabae or Myzus ascalonicus. In attempting tocorrelate biological properties with molecular variations,the RT proteins... |
| Tipo: Journal Article |
Palavras-chave: MILD-YELLOWING-VIRUS; CHLOROSIS-VIRUS; BMYV; BChV; TRANSMISSION EFFICIENCY; VECTOR SPECIFICITY; DETECTION IN VECTOR. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009cb35ecd8&uri=/notices/prodinra1/2010/10/ |
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| Uhde, K.; Kerschbaumer, R.J.; Koenig, R.; Hirsch, S.; Lemaire, O.; Boonham, N.; Roake, W.; Himmler, G.. |
| The detection of Beet necrotic yellow vein virus (BNYVV) instored sugar beets by means of monoclonal antibodies or antibody singlechain fragments (scFv) often poses problems, because the immunodominantC-terminal epitope of the viral coat protein is readily lost due toproteolysis. Clones which produce scFv specific for protease-stableBNYVV epitopes were selected from two naive phage display libraries.Fusion proteins of the scFv with a human IgG kappa chain (expressed fromthe newly designed vector pCL) or with alkaline phosphatase,respectively, allow the ELISA detection of BNYVV even in stored sugarbeets with a sensitivity which was comparable or often higher than thatachieved with polyclonal antibodies |
| Tipo: Journal Article |
Palavras-chave: BNYVV; BEET NECROTIC YELLOW VEIN VIRUS; EPITOPE; COQUE VIRALE; VIRUS DES VEGETAUX; ANTICORPS MONOCLONAL; TEST ELISA. |
| Ano: 2000 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0100016386085053&uri=/notices/prodinra1/2010/11/ |
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| Schellenberger, P.; Demangeat, G.; Lemaire, O.; Ritzenthaler, C.; Bergdoll, M.; Oliéric, V.; Sauter, C.; Lorber, B.. |
| The small icosahedral plant RNA nepovirus Grapevine fanleaf virus (GFLV) is specifically transmitted by anematode and causes major damage to vineyards worldwide. To elucidate the molecular mechanismsunderlying the recognition between the surface of its protein capsid and cellular components of its vector,host and viral proteins synthesized upon infection, the wild type GFLV strain F13 and a naturalmutant (GFLV-TD) carrying a Gly297Asp mutation were purified, characterized and crystallized. Subsequently,the geometry and volume of their crystals was optimized by establishing phase diagrams.GFLV-TD was twice as soluble as the parent virus in the crystallization solution and its crystals diffractedX-rays to a resolution of 2.7 Å. The diffraction limit of... |
| Tipo: Journal Article |
Palavras-chave: VIRUS; GRAPEVINE FANLEAF VIRUS; CRISTALLIZATION; PHASE DIAGRAM; AGAROSE; GEL; SOLUBILITY; VIRUS DU COURT-NOUE. |
| Ano: 2011 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201142bd24bc&uri=/notices/prodinra1/2011/05/ |
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| Registros recuperados: 11 | |
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