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Registros recuperados: 11
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Aphid transmissibility of different European beet polerovirus isolates Inra
Kozlowska-Makulska, A.; Beuve, M.; Syller, J.; Szyndel, M.S.; Lemaire, O.; Bouzoubaa, S.; Herrbach, E..
Different field isolates of the ‘beet poleroviruses’Beet mild yellowing virus (BMYV) and Beetchlorosis virus (BChV) (genus Polerovirus, familyLuteoviridae) collected in France and Poland wereevaluated for transmissibility from and to sugar beetplants by different aphid species. In general, bothBMYV and BChV were efficiently transmitted by Myzus persicae and by a French clone of Macrosiphumeuphorbiae. In contrast, transmissibility of the twopoleroviruses by an English clone of M. euphorbiaewas evidently weaker, although the aphid samplescontained the virus as demonstrated by RT-PCR. Noneof the BMYV or BChV isolates was transmitted byAphis fabae or Myzus ascalonicus. In attempting tocorrelate biological properties with molecular variations,the RT proteins...
Tipo: Journal Article Palavras-chave: MILD-YELLOWING-VIRUS; CHLOROSIS-VIRUS; BMYV; BChV; TRANSMISSION EFFICIENCY; VECTOR SPECIFICITY; DETECTION IN VECTOR.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009cb35ecd8&uri=/notices/prodinra1/2010/10/
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Biological properties and molecular characterization of beet chlorosis virus (BChV) Inra
Hauser, S.; Stevens, M.; Beuve, M.; Lemaire, O..
 Two distinct viruses belonging to the Polerovirus genus, in the family Luteoviridae, have been described as being able to induce mild yellowing on sugar beet: Beet mild yellowing virus (BMYV) and more recently, beet chlorosis virus (BChV). We have analysed biological properties and molecular organisation of two strains of BChV, one collected in England and the second from California. The biological data suggested that BChV displayed a narrower host range compared to BMYV and Beet western yellows virus lettuce isolate (BWYV). The complete genomic RNA sequence of the American isolate BChV-California and the European isolate BChV-2a showed a genetic organisation and expression typical of other Polerovirus members including 6 open reading frames (ORFs)....
Tipo: Journal Article Palavras-chave: BEET CHLOROSIS VIRUS; BCHV; CARACTERISATION MOLECULAIRE; VIRUS DES VEGETAUX; PROPRIETE BIOLOGIQUE.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200016621090487&uri=/notices/prodinra1/2010/11/
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Characterisation of a new potyvirus species infecting meadow saffron (Colchicum autumnale) Inra
Poutaraud, A.; Desbiez, C.; Lemaire, O.; Lecoq, H.; Herrbach, E..
The alkaloids contained in Colchicum autumnale seeds are used in numerous medicines. Good quality seeds are difficult to obtain from this undomesticated plant. Therefore, a research program was set up aiming to cultivate C. autumnale in order to improve alkaloid contents and seed yields. In this context, a collection was established in 1999 by transplanting corms from twelve different locations in Eastern France. However, serious symptoms of necrosis and decay have appeared in this collection since 2001. Electron microscopic observations of plants showing symptoms revealed the presence of filamentous particles and pinwheel-like structures characteristic of the Potyviridae family. Leaves and corms from symptomatic plants were assayed with potyvirus-specific...
Tipo: Journal Article Palavras-chave: COLCHICUM AUTUMNALE; LILIACEAE; PLANTE TOXIQUE; POTYVIRUS; RT-PCR; MSBV; MEADOW SAFFRON BREAKING VIRUS; GENOME VIRAL; VIRUS DES VEGETAUX; ALCALOIDE; TEST ELISA; SEQUENCE NUCLEOTIDIQUE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400031229104456&uri=/notices/prodinra1/2010/10/
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Comparisons of genomic and pathological features among barley and grapevine infecting-isolates of Arabis mosaic virus Inra
Marmonier, A.; Vigne, E.; Komar, V.; Demangeat, G.; Besse, S.; Gugerli, P.; Lemaire, O..
Tipo: Conference Paper Palavras-chave: VIRULENCE; POUVOIR PATHOGENE; HOTE GENOMIC; PATHOLOGICAL FEATURES; BARLEY; GRAPEVINE INFECTING-ISOLATES; ARABIS MOSAIC VIRUS; ARMV-BA; NEPOVIRUS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010aa22413b&uri=/notices/prodinra1/2011/05/
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Detection of leafroll GLRaV-1, 2 and 3 on grapevine rootstocks varieties Inra
Beccavin, I.; Beuve, M.; Lemaire, O.; Grenan, S..
Tipo: Conference Proceedings Palavras-chave: VIRUS DE L'ENROULEMENT.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011a83447d5&uri=/notices/prodinra1/2011/05/
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Genetic structure and variability of virus populations in cross-protected grapevines superinfected by Grapevine fanleaf virus Inra
Vigne, E.; Marmonier, A.; Komar, V.; Lemaire, O.; Fuchs, M..
Recombination was assessed in a vineyard site in which grapevines cross-protected with mild strains GHu of Grapevine fanleaf virus (GFLV) or Ta of Arabis mosaic virus (ArMV) were superinfected with GFLV field isolates following transmission by the nematode vector Xiphinema index. The genetic structure and variability within RNA2 of isolates from grapevines co-infected with GFLV field isolates and either GFLVGHu or ArMV-Ta were characterized to identify intra- and interspecies recombinants. Sequence analysis and phylogenetic relationships inferred intraspecies recombination among GFLV field isolates but not between field isolates and GFLV-GHu. SISCAN analysis confirmed a mosaic structure for two GFLV field isolates for which recombination siteswere located...
Tipo: Journal Article Palavras-chave: ARABIS MOSAIC VIRUS; GRAPEVINE FANLEAF VIRUS; GENETIC STRUCTURE; MOLECULAR DIVERSITY; PHYLOGENY; RNA RECOMBINATION .
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20098313dfaa&uri=/notices/prodinra1/2011/05/
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Improved detection of Beet necrotic yellow vein virus in a DAS ELISA by means of antibody single chain fragments (scFv) which were selected to protease-stable epitopes from phage display libraries Inra
Uhde, K.; Kerschbaumer, R.J.; Koenig, R.; Hirsch, S.; Lemaire, O.; Boonham, N.; Roake, W.; Himmler, G..
 The detection of Beet necrotic yellow vein virus (BNYVV) instored sugar beets by means of monoclonal antibodies or antibody singlechain fragments (scFv) often poses problems, because the immunodominantC-terminal epitope of the viral coat protein is readily lost due toproteolysis. Clones which produce scFv specific for protease-stableBNYVV epitopes were selected from two naive phage display libraries.Fusion proteins of the scFv with a human IgG kappa chain (expressed fromthe newly designed vector pCL) or with alkaline phosphatase,respectively, allow the ELISA detection of BNYVV even in stored sugarbeets with a sensitivity which was comparable or often higher than thatachieved with polyclonal antibodies
Tipo: Journal Article Palavras-chave: BNYVV; BEET NECROTIC YELLOW VEIN VIRUS; EPITOPE; COQUE VIRALE; VIRUS DES VEGETAUX; ANTICORPS MONOCLONAL; TEST ELISA.
Ano: 2000 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0100016386085053&uri=/notices/prodinra1/2010/11/
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"Interactive technology assessment" and Beyond: the field trial of genetically modified grapevines at INRA-Colmar Inra
Lemaire, O.; Moneyron, A.; Masson, J..
Tipo: Journal Article Palavras-chave: OGM; ORGANISME GÉNÉTIQUEMENT MODIFIE; EVALUATION DES TECHNOLOGIES; INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE; DECISION; PARTICIPATION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010536255a6&uri=/notices/prodinra1/2011/01/
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Rootstock genetic engineering for GFLV resistance in grapevine Inra
Goldschmidt, V.; Vigne, E.; Marmonier, A.; Komar, V.; Demangeat, G.; Masson, J.; Lemaire, O..
Tipo: Conference Proceedings Palavras-chave: ROOSTOCK; GENETIC; GFLV; GRAPEVINE FANLEAF DISEASE; NEPOVIRUSES.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201069103b4a&uri=/notices/prodinra1/2011/05/
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Strategies for the crystallization of viruses: Using phase diagrams and gels to produce 3D crystals of Grapevine fanleaf virus Inra
Schellenberger, P.; Demangeat, G.; Lemaire, O.; Ritzenthaler, C.; Bergdoll, M.; Oliéric, V.; Sauter, C.; Lorber, B..
The small icosahedral plant RNA nepovirus Grapevine fanleaf virus (GFLV) is specifically transmitted by anematode and causes major damage to vineyards worldwide. To elucidate the molecular mechanismsunderlying the recognition between the surface of its protein capsid and cellular components of its vector,host and viral proteins synthesized upon infection, the wild type GFLV strain F13 and a naturalmutant (GFLV-TD) carrying a Gly297Asp mutation were purified, characterized and crystallized. Subsequently,the geometry and volume of their crystals was optimized by establishing phase diagrams.GFLV-TD was twice as soluble as the parent virus in the crystallization solution and its crystals diffractedX-rays to a resolution of 2.7 Å. The diffraction limit of...
Tipo: Journal Article Palavras-chave: VIRUS; GRAPEVINE FANLEAF VIRUS; CRISTALLIZATION; PHASE DIAGRAM; AGAROSE; GEL; SOLUBILITY; VIRUS DU COURT-NOUE.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201142bd24bc&uri=/notices/prodinra1/2011/05/
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Viral determinant implicated in the transmission of the Grapevine fanleaf virus by its nematode vector, Xiphinema index Inra
Schellenberger, P.; Demangeat, G.; Andret-Link, P.; Keichinger, C.; Bergdoll, M.; Bron, P.; Lorber, B.; Lemaire, O.; Ritzenthaler, C..
Tipo: Conference Proceedings Palavras-chave: VIRAL TRANSMISSION; GRAPEVINE FANLEAF VIRUS; ITS NEMATODE VECTOR; XIPHINEMA INDEX; RNA.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010c5b4ecd0&uri=/notices/prodinra1/2011/01/
Registros recuperados: 11
Primeira ... 1 ... Última
 

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