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Registros recuperados: 6
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An attempt to channel the transformation of vanillic acid into vanillin by controlling methoxyhydroquinone formation in Pycnoporus cinnabarinus with cellobiose Inra
Lesage-Meessen, L.; Haon, M.; Delattre, M.; Thibault, J.F.; Colonna Ceccaldi, B.; Asther, M..
The effects of adding cellobiose on the transformation of vanillic acid to vanillin by two strains of Pycnoporus cinnabarinus MUCL39532 and MUCL38467 were studied. When maltose was used as the carbon source in the culture medium, very high levels of methoxyhydroquinone were formed from vanillic acid. When cellobiose was used as the carbon source and/or added to the culture medium of P. cinnabarinus strains on day 3 just before vanillic acid was added, it channelled the vanillic acid metabolism via the reductive route leading to vanillin. Adding 3.5 g l−1 cellobiose to 3-day-old maltose cultures of P. cinnabarinus MUCL39532 and 2.5 g l−1 cellobiose to 3-day-old cellobiose cultures of P. cinnabarinus MUCL38467, yielded 510 mg l−1 and 560 mg l−1 vanillin...
Tipo: Journal Article Palavras-chave: PYCNOPORUS CINNABARINUS; METABOLISME ENZYMATIQUE; VANILLINE; ACIDE VANILLIQUE; COMPOSE AMINE; CELLOBIOSE.
Ano: 1997 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9700027779065989&uri=/notices/prodinra1/2010/09/
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Chromogenic substrates for feruloyl esterases Inra
Marmuse, L.; Asther, M.; Navarro, D.; Lesage-Meessen, L.; Asther, M.; Fort, S.; Driguez, H..
Chromogenic mono- and diferuloyl-butanetriol analogs were prepared by chemical syntheses and their efficiency was evaluated as substrates for feruloyl esterases from Aspergillus niger
Tipo: Journal Article Palavras-chave: ACTIVITE ENZYMATIQUE FERULOYL ESTERASE; CHROMOGENIC SUBSTRATE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200895c1f35e&uri=/notices/prodinra1/2008/06/
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Evidence of a new biotransformation pathway of p-coumaric acid into p-hydroxybenzaldehyde in Pycnoporus cinnabarinus Inra
Estrada Alvarado, I.; Lomascolo, A.; Navarro, D.; Delattre, M.; Asther, M.; Lesage-Meessen, L..
Pycnoporus cinnabarinus MUCL39533 was shown to be able to convert p-coumaric acid into p-hydroxybenzaldehyde, a component of high organoleptic note present in natural vanilla aroma. Use of phospholipid-enriched medium led to high-density cultures of P. cinnabarinus, since dry mycelial biomass was increased three-fold as compared to glucose medium. In the presence of phospholipids, 155 mg l–1 p-hydroxybenzaldehyde was produced as the major compound on culture day 13 with a molar yield of 26%. The degradation pathways of p-coumaric acid were investigated. Based on the different metabolites identified, an oxidative side-chain degradation pathway of p-coumaric acid conversion to p-hydroxybenzoic acid was suggested. This acid was further reduced...
Tipo: Journal Article Palavras-chave: PYCNOPORUS CINNABARINUS; AROME NATUREL; BENZALDEHYDE; VANILLINE.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300030628096328&uri=/notices/prodinra1/2010/09/
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Fungal Planet description sheets: 154–213 Naturalis
Crous, P.W.; Wingfield, M.J.; Guarro, J.; Cheewangkoon, R.; Bank, M. van der; Swart, W.J.; Stchigel, A.M.; Cano-Lira, J.F.; Roux, J.; Madrid, H.; Damm, U.; Wood, A.R.; Shuttleworth, L.A.; Hodges, C.S.; Munster, M.; Jesús Yáñez-Morales, M. de; Zúñiga-Estrada, L.; Cruywagen, E.M.; Hoog, G.S. de; Silvera, C.; Najafzadeh, J.; Davison, E.M.; Davison, P.J.N.; Barrett, M.D.; Barrett, R.L.; Manamgoda, D.S.; Minnis, A.M.; Kleczewski, N.M.; Flory, S.L.; Castlebury, L.A.; Clay, K.; Hyde, K.D.; Maússe-Sitoe, S.N.D.; Chen, Shuaifei; Lechat, C.; Hairaud, M.; Lesage-Meessen, L.; Pawłowska, J.; Wilk, M.; Śliwińska-Wyrzychowska, A.; Mętrak, M.; Wrzosek, M.; Pavlic-Zupanc, D.; Maleme, H.M.; Slippers, B.; Mac Cormack, W.P.; Archuby, D.I.; Grünwald, N.J.; Tellería, M.T.; Dueñas, M.; Martín, M.P.; Marincowitz, S.; Beer, Z.W. de; Perez, C.A.; Gené, J.; Marin-Felix, Y.; Groenewald, J.Z..
Novel species of microfungi described in the present study include the following from South Africa: Camarosporium aloes, Phaeococcomyces aloes and Phoma aloes from Aloe, C. psoraleae, Diaporthe psoraleae and D. psoraleae-pinnatae from Psoralea, Colletotrichum euphorbiae from Euphorbia, Coniothyrium prosopidis and Peyronellaea prosopidis from Prosopis, Diaporthe cassines from Cassine, D. diospyricola from Diospyros, Diaporthe maytenicola from Maytenus, Harknessia proteae from Protea, Neofusicoccum ursorum and N. cryptoaustrale from Eucalyptus, Ochrocladosporium adansoniae from Adansonia, Pilidium pseudoconcavum from Greyia radlkoferi, Stagonospora pseudopaludosa from Phragmites and Toxicocladosporium ficiniae from Ficinia. Several species were also...
Tipo: Article / Letter to the editor Palavras-chave: ITS DNA barcodes; LSU; Novel fungal species; Systematics.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/531798
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One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes Naturalis
Stielow, J.B.; Lévesque, C.A.; Seifert, K.A.; Meyer, W.; Irinyi, L.; Smits, D.; Renfurm, R.; Verkley, G.J.M.; Groenewald, M.; Chaduli, D.; Lomascolo, A.; Welti, S.; Lesage-Meessen, L.; Favel, A.; Al-Hatmi, A.M.S.; Damm, U.; Yilmaz, N.; Houbraken, J.; Lombard, L.; Quaedvlieg, W.; Binder, M.; Vaas, L.A.I.; Vu, D.; Yurkov, A.; Begerow, D.; Roehl, O.; Guerreiro, M.; Fonseca, A.; Samerpitak, K.; Diepeningen, A.D. van; Dolatabadi, S.; Moreno, L.F.; Casaregola, S.; Mallet, S.; Jacques, N.; Roscini, L.; Egidi, E.; Bizet, C.; Garcia-Hermoso, D.; Martin, M.P.; Deng, S.; Groenewald, J.Z.; Boekhout, T.; Beer, Z.W. de; Barnes, I.; Duong, T.A.; Wingfield, M.J.; Hoog, G.S. de; Crous, P.W.; Lewis, C.T.; Hambleton, S.; Moussa, T.A.A.; Al-Zahrani, H.S.; Almaghrabi, O.A.; Louis-Seize, G.; Assabgui, R.; McCormick, W.; Omer, G.; Dukik, K.; Cardinali, G.; Eberhardt, U.; Vries, M. de; Robert, V..
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across > 1 500 species (1 931 strains or specimens) and the outcomes of almost twenty thousand (19 577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1–D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial β-tubulin II (TUB2); iv) γ-actin (ACT); v) translation elongation factor 1-α (TEF1α);...
Tipo: Article / Letter to the editor Palavras-chave: DNA barcoding; ITS supplement; Molecular taxonomy; Phylogeny; Species identification; Universal primers.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/588731
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Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application Inra
Record, E.; Asther, M.; Sigoillot, J.C.; Pagès, S.; Punt, P.J.; Delattre, M.; Haon, M.; Van den Hondel, C.A.M.J.J.; Sigoillot, J.C.; Lesage-Meessen, L.; Asther, M..
A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l(-1)), improved FAEA activity 24.5-fold and a yield of 1 g l(-1) of the corresponding protein in the culture medium was achieved. The secreted FAEA was purified 3.5-fold to homogeneity in a two-step purification procedure with a recovery of 69%. The overproduced protein was characterised and presented properties in good agreement with those of native FAEA. The recombinant FAEA was tested for wheat straw pulp...
Tipo: Journal Article Palavras-chave: BLANCHIMENT FERULOYL ESTERASE; WHEAT-STRAW PULP; ENZYMATIC TREATMENT; LACCASE; XYLANASE.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20071bf46680&uri=/notices/prodinra1/2010/09/
Registros recuperados: 6
Primeira ... 1 ... Última
 

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