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Comportements cellulaires et régulation génétique au cours des réactions d'immunité innée chez la moule Mytilus galloprovincialis ArchiMer
Li, Hui.
The mytiliculture is one of the most important aquaculture in the world, however, little is known on the mussel immune system, which plays a critical role in case of infectious diseases. Like all the other invertebrates, our model, the Mediterranean mussel Mytilus galloprovincialis, possesses only an innate immune system considered to be primitive and non-specific. In the present study, our results showed that the mussel immune system (i) is capable of responding differently to a variety of stimulations, suggesting a specific recognition process; (ii) reacts differently according to the geographic origin of the animals, showing an adaptation to their own environment; (iii) is influenced by the season, which explains the seasonal mortalities; (iv) presents...
Tipo: Text Palavras-chave: Mytilus; Haemocyte; Antimicrobial peptides; Polymorphism; Genetic regulation; Innate immunity; Mytilus; Hémocyte; Peptides antimicrobiens; Polymorphisme; Régulation génétique; Immunité innée.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-6241.pdf
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Influence of temperature, salinity and E. coli tissue content on immune gene expression in mussel: Results from a 2005-2008 survey ArchiMer
Li, Hui; Toubiana, Mylene; Monfort, Patrick; Roch, Philippe.
Several bivalves, including mussels, suffered from mortalities particularly in summer. To look for the possible effect of environmental parameters on immune capacities, Mytilus galloprovincialis were collected monthly from August 2005 to July 2008 from the Palavas Laguna, French Mediterranean coast. Q-PCR was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin B and myticin B), in addition to lysozyme and HSP70. House keeping gene was 28S rRNA. Defensin, myticin B and lysozyme appeared more expressed in spring-summer than in winter. In contrast, HSP70 expression was higher in winter. Statistical studies using principal component analysis (PCA) and multiple regression models revealed positive influence of temperature on...
Tipo: Text Palavras-chave: Mollusks; Mytilus; Innate immunity; Seasonal variations; Immune survey; Q PCR; HSP70; Lysozyme; Myticin; Mytilin; Defensin; Antimicrobial peptide.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7345.pdf
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