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| Lima, Maria da Graça de Souza; Universidade Federal de Pelotas; Lopes, Nei Fernandes; Universidade Federal de Pelotas; Zimmer, Paulo Dejalma; Universidade Federal de Pelotas; Meneghello, Geri Eduardo; Universidade Federal de Pelotas; Ferrari, Cibele; Universidade Federal de Pelotas; Mendes, Cristina Rodrigues; Universidade Federal de Pelotas. |
| The present study aimed to identify salinity-tolerant genes in three cultivars (BRS-7 Taim, BRS Querência and BRS Atalanta) of Oryza sativa L. ssp. indica S. Kato and in three cultivars (BRS Bojurú, IAS 12-9 Formosa and Goyakuman) of Oryza sativa L. ssp. japonica S. Kato. Ten days after emergence seedlings were transferred to a greenhouse and placed in a 15L vessel with half strength Hoagland nutrient solution, which was changed every four days, under controlled temperature and humidity. Plants were harvested 56 days after transfer. DNA extraction was carried out by CTAB method and salinity-tolerant genes SOS and CK1 were identified by in silico research. Amplification of gene sequence was performed with in silico primers. Bands were detected by agar gel... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.03.03.00-9 Oryza sativa L.; Cultivars; Salinity-tolerance; Genes; In silico Biologia Molecular. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/15437 |
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| Lima, Maria da Graça de Souza; Universidade Federal de Pelotas; Lopes, Nei Fernandes; Universidade Federal de Pelotas; Zimmer, Paulo Dejalma; Universidade Federal de Pelotas; Meneghello, Geri Eduardo; Universidade Federal de Pelotas; Mendes, Cristina Rodrigues; Universidade Federal de Pelotas; Amarante, Luciano do; Universidade Federal de Pelotas. |
| The southern State of Rio Grande do Sul (RS) is the main rice producer in Brazil with a 60% participation of the national production and 86% participation of the region. Rice culture irrigation system is done by flooding, which leads to soil salinization, a major environmental constraint to production since it alters the plants’ metabolism exposed to this type of stress. The indica cultivar, widely used in RS, has a higher sensitivity to salinity when compared to that of the japonica cultivar in other physiological aspects. Current research analyzes enzymes expression involved in salt-subjected indica and japonica rice cultivars’ respiration. Oryza sativa L. spp. japonica S.Kato (BRS Bojuru, IAS 12-9 Formosa and Goyakuman) and Oryza sativa L. spp. indica... |
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Palavras-chave: 2.00.00.00-6; 2.03.03.00-9; 2.08.00.00-2 electrophoresis; Isoenzyme; Krebs cycle; O. sativa L.; Salinity Fisiologia Vegetal. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8535 |
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