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A Potential Use of Color Ultrasound as a Tool for Reproductive Management: New Observations Using Color Ultrasound Scanning that were not Possible with Imaging Only in Black and White OAK
MIYAMOTO, Akio; SHIRASUNA, Koumei; HAYASHI, Ken-Go; KAMADA, Daichi; KAWASHIMA, Chiho; KANEKO, Etsushi; ACOSTA, Tomas J.; MATSUI, Motozumi; 宮本, 明夫; 白砂, 孔明; 川島, 千帆; 松井, 基純.
Ultrasonography (US) has been applied to the ovary and the uterus of domestic animals from the late 1980s, and established in 1990s as a practical tool for animal production. US made it possible to detect pregnancy at a very early stage and, most importantly, to observe the real-time dynamics of follicular development and hence the discovery of follicular waves. This has greatly contributed to our understanding of ovarian physiology and helped us to develop several "pin-point" protocols for hormonal treatment. While US may not seem to fit preconceived ideas of a "green" technology, it does not contravene environmental priorities, and it is non-invasive ("ethical") and non-hormonal ("clean"). Using the US technology that is now commercially available at a...
Palavras-chave: Color Doppler ultrasound; Blood flow; Ovary; Reproduction; Cow.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2983
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Accumulation of Eosinophils in the Infundibulum of the Bovine Oviduct Just after Ovulation OAK
KOWSAR, Rasoul; JIEMTAWEEBOON, Sineenard; SHIRASUNA, Koumei; SHIMIZU, Takashi; SASAKI, Motoki; KITAMURA, Nobuo; MIYAMOTO, Akio; 清水, 隆; 佐々木, 基樹; 宮本, 明夫.
This study was to investigate the localization and distribution of eosinophils (EOS) in the bovine oviduct throughout the estrous cycle. Histological studies revealed more abundant EOS in the infundibula of the oviducts ipsilateral to the preovulatory dominant follicle and the ovulated ovary. The number of EOS was higher in the infundibula of the oviducts ipsilateral to the ovulated ovary than those of the oviducts contralateral to the ovulated ovary. The infundibula of the oviducts ipsilateral to the preovulatory dominant follicle had higher number of EOS than those of the oviducts ipsilateral to the mid-cycle corpus luteum. The number of EOS in the isthmus, but not in the ampulla, was higher in the outer layers (tunica muscularis and tunica serosa) than...
Palavras-chave: Bovine; Eosinophil; Oviduct; Ovulation.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3947
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An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro OAK
LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫.
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in...
Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946
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Areolae of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis) OAK
SASAKI, Motoki; AMANO, Yoko; HAYAKAWA, Daisuke; TSUBOTA, Toshio; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; TETSUKA, Masafumi; MIYAMOTO, Akio; FUKUI, Yutaka; BUDIPITOJO, Teguh; KITAMURA, Nobuo; 佐々木, 基樹; 手塚, 雅文; 宮本, 明夫; 北村, 延夫.
In this study, we examined the existence and structure of areolae and the steroidogenesis of areolar trophoblast cells in the Antarctic minke whale placenta morphologically and immunohistochemically. Placentas were collected from the 15th, 16th and 18th Japanese Whale Research Program under Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. The opening and cavity of fetal areolae formed by taller columnar trophoblast cells (areolar trophoblast cells) with long microvilli and a bright cytoplasm, as compared with the trophoblast cells of the chorionic villi interdigitating with the endometrial crypts, were recognized in observations of serial sections. The opening of the areolar cavity...
Palavras-chave: Antarctic minke whale; Areola; Balaenoptera bonaerensis; Placenta; Steroidogenesis.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3861
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Changes in Follicular Vascularity during the First Follicular Wave in Lactating Cows OAK
ACOSTA, Tomas J.; HAYASHI, Ken-Go; MATSUI, Motozumi; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫.
Increase in the blood supply to individual follicles appears to be associated with follicular growth rates and the ability to become the dominant follicle, while reduced thecal vascularity appears to be closely associated with follicular atresia. Therefore, this study aimed to determine the real-time changes in the vascularity of the follicle wall during the first follicular wave in cycling Holstein cows. Normally cycling and lactating cows (n=5) were examined by transrectal color Doppler ultrasonography (the sensitivity for velocity: > 2 mm/sec) to determine the changes in the vasculature of the follicle wall (presence or absence of blood flow) and the diameter of follicles. A new follicular wave and ovulation were induced by GnRH injection at 48 h...
Palavras-chave: Blood flow; Follicular wave; Selection; Deviation; Cow.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2979
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Changes in Plasma Metabolic Hormone Concentrations During the Ovarian Cycles of Japanese Black and Holstein Cattle OAK
KAWASHIMA, Chiho; KIDA, Katsuya; HAYASHI, Ken-Go; AMAYA MONTOYA, Carlos; KANEKO, Etsushi; MATSUNAGA, Nobuyoshi; SHIMIZU, Takashi; MATSUI, Motozumi; MIYAKE, Yoh-Ichi; SCHAMS, Dieter; MIYAMOTO, Akio; 川島, 千帆; 木田, 克弥; 清水, 隆; 松井, 基純; 三宅, 陽一; 宮本, 明夫.
The aim of the present study was to investigate the changing profiles of plasma metabolic hormones during the ovarian cycles of beef and dairy cattle. We used 16 non-pregnant, non-lactating Japanese Black beef cattle (6 heifers and 10 cows; parity=2.3 ± 0.8) and 12 multiparous Holstein dairy cows (parity=3.0 ± 0.3). Blood samples for hormonal analysis (growth hormone, GH; insulin-like growth factor-I, IGF-1; insulin; and progesterone, P4) were obtained twice weekly for 40 days before artificial insemination for Japanese Black cattle and from 50 to 100 days postpartum for Holstein cows. Luteal phases were considered normal if the P4 concentrations for at least 3 time points over the course of 7 days remained above 1 ng/ml and at least 2 of the time points...
Palavras-chave: Cattle; Insulin-like growth factor-I; Ovarian cycle; Progesterone.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2984
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Changes in the Messenger RNA Expressions of the Endothelin-1 and Angiotensin Systems in Mature Follicles of the Superovulated Bovine Ovary OAK
SHIMIZU, Takashi; BERISHA, Bajram; SCHAMS, Dieter; MIYAMOTO, Akio; 清水, 隆; 宮本, 明夫.
The aim of the present study was to examine the messenger RNA expressions of the endothelin and angiotensin systems during the periovulatory phase in gonadotrophin releasing hormone (GnRH)-treated cows. Ovaries were collected by transvaginal ovariectomy (n=5 cows/group), and the follicles (n=5, one follicle/cow) were classified into the following groups: before GnRH administration (control, before LH surge), 3-5 h after GnRH (during LH surge), 10 h after GnRH; 20 h after GnRH, 25 h after GnRH (peri-ovulation), and early corpus luteum (CL) (Days 2-3). Expression of mRNA was investigated using quantitative real-time PCR. The expression of angiotensin converting enzyme (ACE) mRNA significantly decreased immediately after onset of the LH surge and remained at...
Palavras-chave: Angiotensin; Bovine; Endothelin-1; Gonadotrophin releasing hormone (GnRH); Ovulation.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2987
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Changes of Progesterone and Endothelin Concentrations in the Peripheral Plasma of Female Calves and Cycling Cows: Effects of PGF2α Injection OAK
OHTANI, Masayuki; OKI, Naoko; TANIMURA, Miyune; KOBAYASHI, Shu-ichi; ACOSTA, Tomas J.; HAYASHI, Kanako; MIYAMOTO, Akio; 宮本, 明夫.
It is well known that an injection of prostaglandin F2α (PGF2α) or its analogue during the mid luteal phase of the estrous cycle induces a rapid decrease in plasma progesterone (P4) concentration, followed by luteolysis in the cow. There is evidence that a potent vasoactive peptide, endothelin-1 (ET-1), is produced in the bovine corpus luteum (CL), and that it is directly involved in luteolysis. We previously found that ET-1 concentrations in the peripheral plasma increase during the period of luteolysis and ovulation in cows. However, it is not clear whether the elevation of peripheral plasma ET-1 concentration observed during luteolysis and ovulation originates exclusively from the ovary and/or CL. Such a profile of plasma ET-1 concentration may be...
Palavras-chave: Endothelin; Progesterone; Plasma; Calves; Cows.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2973
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Classification of Bovine Follicles Based on the Concentrations of Steroids, Glucose and Lactate in Follicular Fluid and the Status of Accompanying Follicles OAK
NISHIMOTO, Hiromi; HAMANO, Seizo; HILL, Glen Alan; MIYAMOTO, Akio; TETSUKA, Masafumi; 宮本, 明夫; 手塚, 雅文.
A simple and clear means to identify the physiological status of follicles is essential for study of follicular biology. In the present study, we verified a novel classification procedure based on analysis of the follicular population and glucose concentration in follicular fluid (FF) as an alternative method to classify bovine follicles. Paired ovaries were collected from heifers, and the number of follicles and stage of the CL were recorded. Follicles were initially divided into the following 3 groups according to diameter and the ratio of E2 and P4 (E/P): E2 active (E-A: E/P≥1), E2 inactive (E-I: E/P<1, ≥8.5 mm) and small follicles (E/P<1, <8.5 mm). E-A follicles were easily identified as E2-rich dominant follicles and were further classified...
Palavras-chave: Bovine; Follicular fluid; Follicle classification; Glucose; Lactate; Steroid.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2828
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Comparison of Bacterial Endotoxin Lipopolysaccharide Concentrations in the Blood, Ovarian Follicular Fluid and Uterine Fluid: A Clinical Case of Bovine Metritis OAK
MAGATA, Fumie; ISHIDA, Yoshikazu; MIYAMOTO, Akio; FURUOKA, Hidefumi; INOKUMA, Hisashi; SHIMIZU, Takashi; 宮本, 明夫; 古岡, 秀文; 猪熊, 壽; 清水, 隆.
We investigated the concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old lactating Holstein cow exhibited continuous fever >39.5°C for more than 2 weeks after normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven follicles showed an extremely high level of LPS (12.40 EU/ml) compared to the other follicles (0.62–0.97 EU/ml). These results might suggest the presence of high concentration of LPS in follicles in cows with postpartum metritis.
Palavras-chave: Dairy cow; Follicle; Lipopolysaccharide; Metritis.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4043
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Differential Effect of Follicle-Stimulating Hormone and Estradiol on Expressions of Vascular Endothelial Growth Factor (VEGF) 120, VEGF164 and Their Receptors in Bovine Granulosa Cells OAK
SHIMIZU, Takashi; JAYAWARDANA, Barana C.; TETSUKA, Masafumi; MIYAMOTO, Akio; 清水, 隆; 手塚, 雅文; 宮本, 明夫.
Vascular endothelial growth factor (VEGF) isoforms (VEGF 120 and VEGF 164) secreted by granulosa cells are involved in thecal angiogenesis during follicular development in the bovine ovary. However, whether the transcript of the VEGF120 and VEGF164 isoforms differs during follicular development in the ovary is still unknown. We first examined the gene expression of VEGF120, VEGF164, fms-like tyrosine kinase (Flt-1), and fetal liver kinase (Flk-1) in the granulosa cells (GCs) and theca cells (TCs) of pre-selection and post-selection follicles (PRF and POF respectively) from the bovine ovary. Then we examined the effects of FSH and estradiol (E2) on these factors in cultured bovine GCs. Messenger RNA (mRNA) expression was quantified using real-time PCR...
Palavras-chave: Estradiol; Fetal liver kinase (Flk-1); Fms-like tyrosine kinase (Flt-1); Follicle-stimulating hormone (FSH); Granulosa cell; Vascular Endothelial Growth factor (VEGF).
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4057
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Distribution of Arteriolovenous Vessels, Capillaries and eNOS Expression in the Bovine Corpus Luteum During the Estrous Cycle : a Possible Implication of Different Sensitivity by Luteal Phase to PGF2α in the Increase of Luteal Blood Flow OAK
SHIRASUNA, Koumei; ASAHI, Takayuki; SASAKI, Motoki; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 佐々木, 基樹; 清水, 隆; 宮本, 明夫.
We have shown that luteal blood flow increases in the peripheral vasculature of the mature corpus luteum (CL) prior to the onset of luteolysis in response to prostaglandin F2α (PGF2α) in the cow, but this phenomenon does not occur in the early CL. We therefore hypothesize that this acute increase of luteal blood flow occurs by vasodilation of large blood vessels due to local release of nitric oxide (NO). In the present study, we characterized the CL vasculature together with endothelial NO synthase (eNOS) expression during the estrous cycle in the cow. Immunohistochemistry was used to quantify the number of arteriolovenous vessels (surrounded with smooth muscle actin-positive smooth muscle cells), capillaries (with von Willebrand Factor-positive...
Palavras-chave: Blood vessels; Corpus luteum; ENOS; Luteolysis.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2989
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Effect of Suckling on the Reproductive Performance and Metabolic Status of Obese Japanese Black Cattle during the Early Postpartum Period OAK
KAWASHIMA, Chiho; KIDA, Katsuya; MATSUHASHI, Miki; MATSUI, Motozumi; SHIMIZU, Takashi; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 川島, 千帆; 木田, 克弥; 松井, 基純; 清水, 隆; 石井, 三都夫; 三宅, 陽一; 宮本, 明夫; 松長, 延吉.
The aim of our study was to investigate the effects of suckling on reproductive performance and metabolic status of obese (mean body condition score of more than 4.0 on a scale of 1-5) maternal Japanese Black cows during early postpartum period. We used 7 postpartum Japanese Black cattle. Four cows were suckled ad libitum (suckled) until completion of their first artificial insemination (AI), while 3 cows were not suckled at all because they were separated from their calves immediately after parturition (non-suckled). Body weight and plasma concentrations of metabolites and hormones were measured from wk 1 to 9 postpartum. Ovarian activity was detected using plasma progesterone concentration, and all cows received their first AI after application of the...
Palavras-chave: Japanese Black cattle; Metabolic status; Obesity; Reproductive performance; Suckling.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2991
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Effect of the Dominant Follicle Aspiration before or after Luteinizing Hormone Surge on the Corpus Luteum Formation in the Cow OAK
HAYASHI, Ken-Go; MATSUI, Motozumi; ACOSTA, Tomas J.; KIDA, Katsuya; MIYAMOTO, Akio; 松井, 基純; 木田, 克弥; 宮本, 明夫.
Luteinizing hormone (LH) surge and follicle rupture act as trigger to start corpus luteum (CL) formation. Thus, we aimed to investigate whether a dominant follicle that has not been exposed to an LH surge can become a functional CL. For this purpose, follicular fluid from the dominant follicles (DF) of cows was aspirated before or after a GnRH-induced LH surge, and subsequent CL formation was observed. Holstein cows were divided into four groups as follows: Luteal phase, a DF was aspirated 7 days after GnRH injection; Pre-LH surge, a DF was aspirated 42 h after PGF2α injection during the mid luteal phase; Post-LH surge, a DF was aspirated 24 h after GnRH injection following PGF2α; and Intact follicle, ovulation was induced by GnRH injection after PGF2α....
Palavras-chave: Corpus luteum formation; Cow; Follicle aspiration; LH surge.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2982
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Endothelin-1 downregulates sperm phagocytosis by neutrophils in vitro: a physiological implication in bovine oviduct immunity OAK
MAREY, Mohamed Ali; YOUSEF, Mohamed Samy; LIU, Jinghui; MORITA, Kazuhiro; SASAKI, Motoki; HAYAKAWA, Hiroyuki; SHIMIZU, Takashi; ELSHAHAWY, Ibrahim I.; MIYAMOTO, Akio; 佐々木, 基樹; 清水, 隆; 宮本, 明夫.
The oviduct is an active contractile tube that provides the proper environment for sperm transport, capacitation and survival. Oviductal contractions are regulated by autocrine/paracrine secretion of several factors, such as prostaglandins (PGs) and endothelin-1 (EDN-1). We have previously shown that during the preovulatory stage, sperm are exposed to polymorphonuclear neutrophils (PMNs) in the bovine oviduct, and the bovine oviduct epithelial cells (BOECs) secrete molecules including PGE2 that suppress sperm phagocytosis by PMNs in vitro. In this study, we investigated the possible effects of EDN-1 on the phagocytic activity of PMNs toward sperm. The local concentrations of EDN-1 in oviduct fluid and BOEC culture medium ranged from 10–10 to 10–11 M as...
Palavras-chave: Endothelin-1; Oviduct; Phagocytosis; Polymorphonuclear neutrophils (PMN); Sperm.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4310
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Exposure of C57BL/6J Male Mice to an Electric Field Improves Copulation Rates with Superovulated Females OAK
HORI, Takuya; YAMSAARD, Thicomeporn; UETA, Yoshiko Yanagimoto; HARAKAWA, Shinji; KANEKO, Etsushi; MIYAMOTO, Akio; XUAN, Xuenan; TOYODA, Yutaka; SUZUKI, Hiroshi; 宮本, 明夫; 玄, 学南; 鈴木, 宏志.
It is well-known that there are considerable strain differences in the relative copulation rates between male and superovulated female mice. In particular, the C57BL/6J strain of mice has a lower rate of successful copulation. We examined the effect of exposure to an electric field on sexual behavior in C57BL/6J male mice. When C57BL/6J males were exposed to a 50 Hz, 45 kV/m electric field for 30 min per day for 11 days and placed in a cage with a superovulated female of the same strain, the successful copulation rates of males was significantly improved compared with unexposed males (P<0.05). These results suggest that the exposure of C57BL/6J male mice to an electric field improves their sub-fertility activity in mating with superovulated females.
Palavras-chave: Mice; Male; Electric field; Copulation; Superovulation; Sexual behavior.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3021
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Expression of mRNA for Cell Adhesion Molecules in the Bovine Corpus Luteum During the Estrous Cycle and PGF2α-Induced Luteolysis OAK
SHIRASUNA, Koumei; WATANABE, Sho; NAGAI, Kaya; SASAHARA, Kiemi; SHIMIZU, Takashi; RICKEN, Albert M.; SPANEL-BOROWSKI, Katharina; MIYAMOTO, Akio; 白砂, 孔明; 清水, 隆; 宮本, 明夫.
Cell-to-cell interaction via cell contact-dependent pathway is essentially important for maintenance and regulation of corpus luteum (CL) integrity and its physiological actions. The objective of the present study was to evaluate the mRNA expression of the cell adhesion molecules (CAMs) that are constituent factors of gap junctions [connexin (Cx) 43] and adherence junctions (VE-, E-, N-cadherin) in two types of endothelial cells from the mid CL and in CL tissue during the estrous cycle and PGF2α-induced luteolysis in the cow. Specific mRNA expression for Cx43 and N-cadherin was detected in cytokeratin-positive (CK+) and cytokeratin-negative (CK-) luteal endothelial cells (EC) and fully luteinized granulosa cells (LGC). E-cadherin mRNA was expressed in...
Palavras-chave: Cadherin; Connexin; Corpus luteum; Cow; Luteolysis.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2988
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Expression of mRNA for the Angiopoietin-Tie System in Granulosa Cells during Follicular Development in Cows OAK
HAYASHI, Ken-Go; BERISHA, Bajram; MATSUI, Motozumi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫.
Recent findings indicate that the changing profile of angiopoietins (ANPT) and their receptor Tie2 are closely associated with development and regression of the vascular network in the cyclic ovary. We previously reported that mRNA expression for the ANPT-Tie system in theca interna changes during bovine follicular development and atresia, and both ANPTs affect steroidogenesis in the preovulatory follicle. The aim of this study was to investigate mRNA expression for ANPT1, ANPT-2 and Tie2 in granulosa cells (GC) during follicular development in the cow. Bovine follicles were classified according to the estradiol-17β (E2) concentration in follicular fluid (FF) as follows: (1) E2<0.5, (2) 0.5<E2<5, (3) 5<E2<20, (4) 20<E2<180 and (5)...
Palavras-chave: Follicular development; Angiopoietin-Tie system; Granulosa cell; Cow.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2975
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Expression of Pin1, a peptidyl-prolyl isomerase, in the ovary of eCG/hCG-treated immature female mice. OAK
SHIMIZU, Takashi; AKIYAMA, Hirotada; ABE, Yasuyuki; SASADA, Hiroshi; SATO, Eimei; MIYAMOTO, Akio; UCHIDA, Takafumi; 清水, 隆; 宮本, 明夫.
Protein phosphorylation on certain serine or threonine residues preceding proline (Ser/Thr-Pro) is a pivotal signaling mechanism in diverse cellular processes. Pin1 is a highly conserved enzyme that isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby inducing conformational changes. Although much protein is phosphorylated in the ovary, the role of Pin1 in the ovary is still unknown. The purpose of this study is to investigate the effects of gonadotropins on protein and mRNA expression of Pin1 in mice ovaries. Quantitative PCR analysis showed that the expression of Pin1 mRNA significantly increased in the ovaries of equine chorionic gonadotropin (eCG)-treated mice compared with those of untreated mice (P<0.05). However,...
Palavras-chave: E2F; Follicle development; Gonadotropin; Ovary; Pin1.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4056
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Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone OAK
TETSUKA, Masafumi; NISHIMOTO, Hiromi; MIYAMOTO, Akio; OKUDA, Kiyoshi; HAMANO, Seizo; 手塚, 雅文; 宮本, 明夫.
Glucocorticoids modulate ovarian function in cattle. However, their regulatory mechanisms have not been fully elucidated. In the present study, we examined gene expression of two glucocorticoid-metabolizing enzymes, a bidirectional 11β-HSD type 1 (11HSD1) and a dehydrogenase 11β-HSD type 2 (11HSD2), and glucocorticoid receptor (GR) in bovine follicles during follicular maturation and atresia. Granulosa cells (GCs) and theca interna layers (TIs) were harvested from follicles classified as small growing, dominant, preovulatory, early atretic and late atretic follicles. The expression levels of 11HSD1, 11HSD2 and GR mRNA were quantified by real-time PCR. In the healthy follicles, expression of 11HSD1 mRNA increased as follicles matured, both in GCs and TIs. A...
Palavras-chave: Bovine; Cortisol; 11β-HSD; Follicle; Glucocorticoid receptor; MRNA.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2810
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