SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

MOREIRA, G. C. M.; PÉRTILLE, F.; GODOY, T. F.; BRASSOLOTI, R.; BOSCHIERO, C.; LEDUR, M. C.; COUTINHO, L. L..

2014

Discovery of SNPs potentially associated with fatness in a QTL region on chicken chromosome 3.

Palavras-chave: 600K SNP chip; Chicken; Poultry; Genetic.

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1014218

MOREIRA, G. C. M.; GODOY, T. F.; BOSCHIERO, C.; GHEYAS, A. A.; GASPARIN, G.; PADUAN, M.; ANDRADE, S. C. S.; MONTENEGRO, H.; BURT, D. W.; LEDUR, M. C.; COUTINHO, L. L..

2014

Gene co-expression analysis indicates potential pathways and regulators of beef tenderness in Nellore cattle.

Palavras-chave: Abdominal fat; Re sequencing; Polymorphisms.

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1032943

GONÇALVES, T. M.; REGITANO, L. C. de A.; KOLTES, J. E.; CESAR, A. S. M.; ANDRADE, S. C. da S.; MOURAO, G. B.; GASPARIN, G.; MOREIRA, G. C. M.; FRITZ-WATERS, E.; REECY, J. M.; COUTINHO, L. L..

Beef tenderness, a complex trait affected by many factors, is economically important to beef quality, industry, and consumer?s palatability. In this study, RNA-Seq was used in network analysis to better understand the biological processes that lead to differences in beef tenderness. Skeletal muscle transcriptional profiles from 24 Nellore steers, selected by extreme estimated breeding values (EBVs) for shear force after 14 days of aging, were analyzed and 22 differentially expressed transcripts were identified. Among these were genes encoding ribosomal proteins, glutathione transporter ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4), and synaptotagmin IV (SYT4). Complementary co-expression analyses using Partial Correlation with Information...

Genome-wide association studies reveal genomic windows and candidate genes related to fat deposition in chickens.

Palavras-chave: Qualidade da carne; Maciez da carne; Carne; Gado de Corte; Beef; Beef cattle; Beef quality.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106256

MOREIRA, G. C. M.; CESAR, A. S. M.; GODOY, T. F.; BOSCHIERO, C.; LEDUR, M. C.; GARRICK, D. J.; MOURA, A. S. A. M. T.; COUTINHO, L. L..

2016

Genome-wide association study for femur-related traits in broilers.

Palavras-chave: Genome-wide; Chickens.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1045625

ONO, R. K.; IBELLI, A. M. G.; CANTAO, M. E.; PEIXOTO, J. de O.; SOLLERO, B. P.; SUREK, D.; MOREIRA, G. C. M.; GODOY, T. F.; COUTINHO, L. L.; LEDUR, M. C..

Abstract: Due to the intense selection for heavier and faster growing broilers, metabolic disorders such as skeletal problems became a worldwide concern. Advances in genome-wide association study (GWAS) methodologies increased the possibility of elucidating the genetic architecture controlling bone integrity traits. Therefore, the aim of this study was to perform a GWAS to identify potential genetic markers and candidate genes associated with femur traits in a paternal broiler line developed by Embrapa. To this, three femur bone-related traits were evaluated in 1,433 chickens: dry matter (FDM), ash content (FAC) and breaking strength (FBS). Chickens were genotyped using the 600K Affymetrix® Axiom® HD panel. A total of 16 regions associated to FAC, being a...

Genome-wide characterization of genetic variations and regions under selection in broilers and layer chickens.

Palavras-chave: Integridade óssea; Características do fêmur; Associação genômica; QTLs; Melhoramento Genético Animal; Frango de Corte; Formação Óssea; Fêmur; Animal genetic resources; Broiler chickens; Bone metabolism; Quantitative trait loci.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093205

BOSCHIERO, C.; MOREIRA, G. C. M.; GHEYAS, A.; GODOY, T. F.; GASPARIN, G.; MARIANI, P. D. S. C.; PADUAN, M..

Abstract: Background Meat and egg-type chickens have been selected for several generations for different traits. Artificial and natural selection for different phenotypes can change frequency of genetic variants, leaving particular genomic footprints throghtout the genome. Thus, the aims of this study were to sequence 28 chickens from two Brazilian lines (meat and white egg-type) and use this information to characterize genome-wide genetic variations, identify putative regions under selection using Fst method, and find putative pathways under selection.

Identification of CNVs on the genome of Brazilian chicken lines.

Palavras-chave: Fat deposition; Genetic variants; Next generation sequencing; Genoma; Frango de Corte; Linhagem; Ovo; Melhoramento Genético Animal; Polimorfismo Genético; Single nucleotide polymorphism; Egg industry; Broiler chickens; Genome; Poultry carcasses.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1091368

GODOY, T. F.; MOREIRA, G. C. M.; SILVA, V. H.; BOSCHIERO, C.; LEDUR, M. C.; MOURA, A. S. A. M. T.; COUTINHO, L. L..

2016

Identification of mutations in a QTL region on chromosome 3 associated with fatness in chickens.

Palavras-chave: CNVs; Genome; Chicken.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1045633

MOREIRA, G. C. M.; GODOY, T. F.; BOSCHIERO, C.; LEDUR, M. C.; GASPARIN, G.; ANDRADE, S. C. da S.; PADUAN, M.; COUTINHO, L. L..

2013

Identification of polymorphisms in a QTL region on chicken chromosome 2 associated with muscle deposition.

Palavras-chave: Mutations segregating; QTL; Galinha; Melhoramento genético animal; Chickens; Quantitative trait loci.

Ano: 2013

URL: http://www.alice.cnptia.embrapa.br/handle/doc/971381

GODOY, T. F.; MOREIRA, G. C. M.; BOSCHIERO, C.; LEDUR, M. C.; GASPARIN, G.; ANDRADE, S. C. da S.; PADUAN, M.; COUTINHO, L. L..

2013

Identification of putative regulatory regions and transcription factors associated with intramuscular fat content traits.

Palavras-chave: Galinha; Polimorfismo genético; Chickens; Genetic polymorphism; Quantitative trait loci.

Ano: 2013

URL: http://www.alice.cnptia.embrapa.br/handle/doc/971356

CESAR, A. S. M.; REGITANO, L. C. de A.; REECY, J. M.; POLETI, M. D.; OLIVEIRA, P. S. N.; OLIVEIRA, G. B. de; MOREIRA, G. C. M.; MUDADU, M. de A.; TIZIOTO, P. L.; KOLTES, J. E.; Fritz-Waters, E.; KRAMER, L.; GARRICK, D.; BEIKI, H.; GEISTLINGER, L.; MOURÃO, G. B.; ZERLOTINI NETO, A.; COUTINHO, L. L..

Background: Integration of high throughput DNA genotyping and RNA-sequencing data allows for the identification of genomic regions that control gene expression, known as expression quantitative trait loci (eQTL), on a whole genome scale. Intramuscular fat (IMF) content and carcass composition play important roles in metabolic and physiological processes in mammals because they influence insulin sensitivity and consequently prevalence of metabolic diseases such as obesity and type 2 diabetes. However, limited information is available on the genetic variants and mechanisms associated with IMF deposition in mammals. Thus, our hypothesis was that eQTL analyses could identify putative regulatory regions and transcription factors (TFs) associated with...

Integration of GWAS, CNV and sele ction signature reveals candidate genes for abdominal fat regulation in chickens.

Palavras-chave: Expressão gênica; EQTL; Ácidos graxos; Doenças metabólicas; Expression quantitative trait loci; Gene expression; Fatty acids; Mammals; Metabolic diseases.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102203

MOREIRA, G. C. M.; GODOY, T. F.; BOSCHIERO, C.; CESAR, A. S. M.; REECY, J. M.; LEDUR, M. C.; GARRICK, D.; SILVA, V. H. da; CROOIJAMANS, R. P.; GROENEN, M. A. M.; COUTINHO, L. L..

Abstract: Carcass fat content is an economically important trait in commercial chickens. The use of genome-wide high density SNPs may improve the power and resolution to identify QTLs, putative candidate genes and copy number variations (CNVs), for selection programs. The main goal of this study was to identify genomic windows and putative candidate genes for carcass fat content. We checked the overlap of QTL with regions demonstrating signatures of selection and inherited CNVs identified in the same population. A total of 497 42 day-old chickens from the EMBRAPA F2 Chicken Resource Population developed for QTL studies were genotyped with the 600K SNP genotyping array (Affymetrix®), and phenotyped for carcass fat content weight (CFCW) and carcass fat...

Investigation for high-impact SNPs on important genes for muscle growth characteristics in broilers.

Palavras-chave: Genotyping by sequencing; Analyses were performed with GenSel; Selection signatures; 600k snp genotyping array; Melhoramento Genético Animal; Polimorfismo Genético; Frango de Corte; Carcaça; Gordura; Genoma; Animal genetic resources; Broiler chickens; Carcass characteristics; Abdominal fat; Genetic polymorphism; Single nucleotide polymorphism.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093261

PETRY, B.; MOREIRA, G. C. M.; JORGE, E. C.; LEDUR, M. C.; COUTINHO, L. L..

bitstream/item/179856/1/final8848.pdf

MiRNAs differentially expressed in skeletal muscle of animals with divergent estimated breeding values for beef tenderness.

Palavras-chave: Peito de frango; SNP; Melhoramento Genético Animal; Frango de Corte; Marcador Genético; Seleção Genética; Carcaça; Ganho de Peso; Animal genetic resources; Broiler chickens; Breast meat; Liveweight gain.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093333

KAPPELER, B. I. G.; REGITANO, L. C. de A.; POLETI, M. D.; CESAR, A. S. M.; MOREIRA, G. C. M.; GASPARIN, G.; COUTINHO, L. L..

MicroRNAs (miRNAs) are small noncoding RNAs of approximately 22 nucleotides, highly conserved among species, which modulate gene expression by cleaving messenger RNA target or inhibiting translation. MiRNAs are involved in the regulation of many processes including cell proliferation, differentiation, neurogenesis, angiogenesis, and apoptosis. Beef tenderness is an organoleptic characteristic of great influence in the acceptance of meat by consumers. Previous studies have shown that collagen level, marbling, apoptosis and proteolysis are among the many factors that affect beef tenderness. Considering that miRNAs can modulate gene expression, this study was designed to identify differentially expressed miRNAs that could be modulating biological processes...

Novel polymorphisms in the PLIN2 gene of Nellore cattle.

Palavras-chave: Qualidade da carne; Bta-miR; Shear force; Gado de Corte; Carne; Bos Indicus; Beef cattle; Beef; Beef quality; MicroRNA.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106070

DEFANT, H.; MEIRA, A. N.; COUTINHO, L. L.; REGITANO, L. C. de A.; POLETI, M. D.; MOREIRA, G. C. M.; PADUAN, M.; MARIANI, P.; ZERLOTINI NETO, A.; MOURÃO, G. B.; CESAR, A. S. M..

ABSTRACT. Genetic variations in genes involved in lipid storage, which also may interfere with important phenotypic traits such as intramuscular fat deposition in cattle, can represent useful markers in marker assisted selection programs. An important candidate gene is perilipin 2 (PLIN2) that plays a role in the capture of long chain fatty acids and in the formation and stabilization of lipid droplets in different tissues including muscle. As reported by our group, PLIN2 expression level was associated with trans-eQTL (expression quantitative loci distant of gene associated) at chr3: 87, 253, 086 in Nellore cattle, where animals with different genotypes showed different expression level of PLIN2 among the 193 animals and the co-expression analysis...

Single nucleotide polymorphism associated with breast trait in chickens.

Palavras-chave: Perilipin 2; PLIN2; Polimorfismo de nucleotídeo único; SNP; Processos biológicos; Gordura intramuscular; Gota lipídica; Expressão gênica; Biological process; Lipid droplet; Variants; Gado; Bos Indicus; Nellore; Cattle; Single nucleotide polymorphism; Gene expression; Intramuscular fat.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119464

TREVISOLI, P. A.; MOREIRA, G. C. M.; BOSCHIERO, C.; PETRINI, J.; MOURÃO, G. B.; LEDUR, M. C.; COUTINHO, L. L..

bitstream/item/179866/1/final8849.pdf

SNP discovery in a QTL region associated with breast muscle deposition on chicken chromosome 2.

Palavras-chave: Peito de frango; Melhoramento Genético Animal; Frango de Corte; Polimorfismo Genético; Gene; Genoma; Animal genetic resources; Broiler chickens; Breast meat; Weight gain; Single nucleotide polymorphism.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1093336

BOSCHIERO, C.; GODOY, T. F.; MOREIRA, G. C. M.; GHEYAS, A. A.; GASPARIN, G.; PADUAN, M.; ANDRADE, S. C. S.; MONTENEGRO, H.; BURT, D. W.; LEDUR, M. C.; COUTINHO, L. L..

2014