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Registros recuperados: 12
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Aflatoxin M1 in the urine of non-carriers and chronic carriers of hepatitis B virus in Maringa, Brazil BJPS
Giolo,Marcel Padovani; Oliveira,Christiane Minervino de; Bertolini,Dennis Armando; Lonardoni,Maria Valdrinez Campana; Gouveia,Matheus Sampaio; Netto,Daisy Pontes; Nixdorf,Suzana Lucy; Machinski Junior,Miguel.
Exposure to aflatoxins (AFs) in the diet may favour the development of hepatocellular carcinoma (HCC) and the acute exacerbation of hepatitis in chronic hepatitis B virus (HBV) carriers. Measurement of biomarkers such as aflatoxin M1 (AFM1), a metabolite of aflatoxin B1 (AFB1), in urine allows for the assessment of populations exposed to aflatoxins. The aim of this study was to investigate the occurrence of aflatoxin M1 in the urine of HBV carrier and non-carrier patients. One group included 43 randomly selected HBV carriers treated at two hospitals in the city of Maringa, Brazil, from March to June 2008. Control group consisted of 29 healthy adult volunteers with anti-HBs positive and HBsAg negative test results. Detection of AFM1 was performed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxins; Biomarkers; Hepatitis B; Hepatocellular carcinoma; Urina/análise toxicológica; Epidemiology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300011
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Aflatoxins, ochratoxin A and zearalenone in maize-based food products BJM
Sekiyama,Beatriz Leiko; Ribeiro,Alessandra Braga; Machinski,Paulo André; Machinski Junior,Miguel.
The occurrence of aflatoxins B1 (AFB1), B2 (AFB2 ), G1 (AFG1 ) and G2 (AFG2 ), ochratoxin A (OA) and zearalenone (ZEA) was evaluated in 121 maize-based food samples collected in the commerce of Maringá City, Paraná State, Brazil. The study was carried out between January 2002 and February 2003. Thin-layer chromatographic method was used to determine the mycotoxins. The recovery averages were 106.6%, 109.4%, 106.6%, 109.4%, 101.8% and 101.7% to AFB1, AFB2, AFG1, AFG2, OA and ZEA, respectively. Three samples (2.5%) were positive to AFB1 (8 to 59 µg/kg), two (1.7%) to AFB2 (2.4 µg/kg), one (0.8%) to OA (64 µg/kg) and one (0.8%) to ZEA (448 µg/kg). The greater frequency of positive samples and also the highest concentration of AFB1 were found in popcorn...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxins; Risk; Brazilian products derived from maize.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300016
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Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica serotype Typhimurium BJPS
Trevisan,Daliah Alves Coelho; Silva,Alex Fiori da; Negri,Melyssa; Abreu Filho,Benício Alves de; Machinski Junior,Miguel; Patussi,Eliana Valéria; Campanerut-Sá,Paula Aline Zanetti; Mikcha,Jane Martha Graton.
ABSTRACT The present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and the time-kill curve and scanning electron microscopy (SEM) were performed to evaluate antibacterial activity. Antibiofilm activity was evaluated by quantifying total biomass using crystal violet assay, and metabolic activity was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The action of carvacrol against preformed biofilm on polypropylene and stainless steel was also evaluated by colony counting and SEM. The MIC and MBC was 312 µg mL-1. Carvacrol at MIC and 2 x MIC eliminated cells...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carvacrol; Biofilm; Antibacterial activity; Salmonella Typhimurium..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100612
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Contribution of environmental factors in the formation of biofilms by Alicyclobacillus acidoterrestris on surfaces of the orange juice industry Ciência Rural
Dutra,Tatiane Viana; Prado,Daniela Biral do; Anjos,Márcia Maria dos; Machinski Junior,Miguel; Mikcha,Jane Martha Graton; Abreu Filho,Benício Alves de.
ABSTRACT: The objective of this study was to evaluate the effect of the initial microbial load, temperature and contact time on the biofilm formation of Alicyclobacillus acidoterrestris on stainless steel and natural food-grade rubber using orange juice as culture medium. The low initial load of A. acidoterrestris (2 log CFU/mL) led to biofilm formation on the stainless steel surface after 48 h of contact at 28 ºC and after 24 h at 45 ºC, and on natural food-grade rubber surface after 48 h of contact at both temperatures. The high initial microbial load (5 log CFU/mL) led to biofilm formation on stainless steel after 4 h of contact at 28 °C and 45 °C, while biofilm was formed on natural food-grade rubber after 8 h of contact at 28 °C and 4 h at 45 °C. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Concentrated orange juice; Stainless steel; Natural food-grade rubber; Spores; Biofilm.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300753
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Efeito dos extratos aquoso e oleoso de Nim [Azadirachta indica A. Juss (Meliaceae)] na produção de patulina em maçãs contaminadas por Penicillium expansum Ciência Rural
Arroteia,Carla Cristina; Kemmelmeier,Carlos; Machinski Junior,Miguel.
A Região Sul do Brasil é grande produtora de maçã, sendo 80% destinada ao consumo in natura. As micotoxinas são metabólitos secundários de fungos presentes na cadeia alimentar como contaminantes, causando diversos efeitos toxicológicos e imunológicos. Considerando que a patulina seja uma micotoxina produzida por Penicillium expansum, principal contaminante da maçã propôs-se investigar a ação de extratos aquoso e oleoso de Nim (Azadirachta indica) em maçã artificialmente contaminada. Foram testados dois tipos de extratos: o extrato aquoso obtido de maceração de folhas de Nim nas concentrações de 5, 10, 20 e 30%, e o extrato oleoso comercial (DalNeem®), obtido de sementes de Nim, nas concentrações de 0,125, 0,25, 0,5, 1, 2 e 5%. Os extratos aquosos não...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azadirachta indica; Maçã; Nim; Patulina; Penicillium expansum.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600002
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Intralaboratory optimization and validation of a method for patulin determination in grapes by Thin-Layer Chromatography BJM
Oliveira,Renata Cabrera de; Bando,Érika; Machinski Junior,Miguel.
The aim of this work was to optimize and validate, by intralaboratorial procedures, a method for the determination of patulin in grapes by thin-layer chromatography. The steps of extraction, cleanup, detection and quantification were optimized. For the validation of the method, recovery assays with standard solutions and artificially contaminated samples were carried out. The mean recovery and the variation coefficient were 65.4% and 7.58%, respectively. The optimized conditions were: 50 mL of grape juice, three extraction stages (with 100 mL of ethyl acetate in the first stage and 50 mL in second and third stages), and 100 µL of ethanol to solubilize the extract. The solvent-system used was toluene - ethyl acetate - formic acid (6:3:1), and 0.5% MBTH in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Patulin; Mycotoxins; Grape; Thin-layer chromatography.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200023
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Maize (Zea Mays L) landraces from the southern region of Brazil: contamination by Fusarium sp, zearalenone, physical and mechanical characteristics of the kernels BABT
Oliveira,Tatiana Roselena de; Jaccoud-Filho,David de Souza; Henneberg,Luciana; Michel,Milton Domingos; Demiate,Ivo Mottin; Pinto,Alessandra Teixeira Barbosa; Machinski Junior,Miguel; Barana,Ana Cláudia.
This work had as objectives the study of the physical and mechanical characteristics of maize kernels in relation to the contamination by Fusarium sp and by zearalenone in twenty landraces of maize from the southern region of Brazil. From the analyzed samples, 60 % has been considered to have a hard endosperm type and 40 % an intermediary one. A correlation between the physical and mechanical variables was observed as an indication that the higher is the proportion of hard endosperm, more dense will be the kernel and more force for its rupture will be necessary. The level of contamination by Fusarium sp was between 5.5 and 24.75% among the analyzed grains, correlating positively with the flotation index, indicating that the landraces of maize with a softer...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Texture of endosperm; Corn landraces; Mycotoxins.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700002
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Occurrence and exposure assessment to aflatoxins in peanuts commercialized in the northwest of Parana, Brazil Ciência Rural
Santos,Alini Cristini dos; Souza,Aline Amenencia de; Silva,Milena Veronezi; Nerilo,Samuel Botião; Souza,Alexandra Perdigão Maia de; Bando,Érika; Machinski Junior,Miguel.
ABSTRACT: Peanuts are a nutritious food consumed worldwide. Some species of the genus Aspergillus, producers of aflatoxins, colonize peanuts. Human exposure to aflatoxins occurs by ingesting contaminated foods. The objective of this paper was to assess the occurrence and dietary exposure of the population to aflatoxins in peanuts commercially available in Maringa, Brazil, from May 2013 to April 2017. A total of 104 peanut samples were evaluated by thin-layer chromatography and confirmed by derivatization with trifluoroacetic acid. The contamination rate detected was 24.0% in average concentration of 13.4µg/kg. Twenty positive samples (19.2%) exceeded the maximum level permitted in Brazil for the sum of four aflatoxins. Estimated probable daily intake was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxins; Aspergillus flavus; Peanuts; Risk assessment; Public health.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600750
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Occurrence of zearalenone in wheat- and corn-based products commercialized in the State of Paraná, Brazil BJM
Almeida-Ferreira,Giovanna Caputo; Barbosa-Tessmann,Ione Parra; Sega,Rose; Machinski Junior,Miguel.
The productivity of wheat and corn crops depends on climatic conditions and resistance against phytopathogenic fungi such as those of the genus Fusarium. Some species of this genus produce zearalenone (ZEA), a mycotoxin with hyperestrogenic effects. The objective of this study was to investigate the presence of ZEA in samples of cracked wheat (n = 109), popcorn (n = 51) and corn grits (n = 50) commercialized in the State of Paraná, Brazil. Commercial samples of each crop were collected between September 2007 and June 2008 and analyzed by thin-layer chromatography. The method used for detection of the mycotoxin in wheat and corn derivatives presented a recovery rate of 94.5% and 99.5%, respectively, detection limit of 40 μg.kg-1 and quantification limit of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat; Corn; Zearalenone; Thin-layer chromatography; Mycotoxins.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200005
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Ocorrência de patulina em uva fina (Vitis vinifera L. cv. "Rubi") com sinais de podridão ácida Ciência Rural
Caldas,Glória Maria Menezes; Oliveira,Renata Cabrera de; Tessmann,Dauri José; Machinski Junior,Miguel.
A podridão ácida é uma patologia das videiras, de alta freqüência nos períodos quentes e chuvosos, em conseqüência do desenvolvimento de fungos e bactérias em seus frutos. Um dos gêneros de fungos responsáveis por esta doença é o Penicillium spp., que poderá levar à produção de micotoxinas, como a patulina. Neste trabalho, foi realizada uma investigação sobre a podridão ácida e a ocorrência de patulina em uvas Itália (Vitis vinifera L. cv. Rubi) cultivadas no município de Marialva, Brasil. Foram avaliados os seguintes tratamentos: (I) testemunha sem sintomas de podridão ácida; (II) amostras com sintomas, sem a remoção das bagas infectadas; (III) amostras com sintomas, com a remoção das bagas infectadas, (IV) amostras com sintomas da doença, com a remoção...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Patulina; Videira; Fitopatologia; Micotoxinas.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000100003
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Pesticide use and cholinesterase inhibition in small-scale agricultural workers in southern Brazil BJPS
Nerilo,Samuel Botião; Martins,Fernanda Andrade; Nerilo,Luciana Botião; Salvadego,Valter Eduardo Cocco; Endo,Renan Yoshio; Rocha,Gustavo Henrique Oliveira; Mossini,Simone Aparecida Galerani; Janeiro,Vanderly; Nishiyama,Paula; Machinski Junior,Miguel.
A controlled cross-sectional study of family growers of fruit and vegetables was conducted between October 2009 and October 2010 to characterize the use of pesticides, establish the socio-demographic profile, and analyze cholinesterase activity in small-scale agricultural workers in Southern Brazil. Data was collected for 173 workers and 179 controls. A structured questionnaire was applied collecting socio-demographic information and determining knowledge and work practices in relation to pesticide use. The benchmarks for total cholinesterase (ChEs) and butyrylcholinesterase (BuChE) were obtained from the average enzymatic activity of the occupationally unexposed group (control). The mean age of the exposed population was 40.0 ± 11.2 years. The symptoms...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pesticides/use; Pesticides/occupational exposure; Cholinesterase/evaluation/exposure to pesticides; Agricultural workers/exposure to pesticides; Environmental health; Brazil/southern/environmental health.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400783
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Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat Ciênc. Tecnol. Aliment.
Faria,Carla Bertechini; Almeida-Ferreira,Giovana Caputo; Gagliardi,Karina Bertechine; Alves,Tatiane Cristina Albuquerque; Tessmann,Dauri José; Machinski Junior,Miguel; Barbosa-Tessmann,Ione Parra.
The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bulgur wheat; Fusarium graminearum; PCR; Molecular detection; Food protection.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030
Registros recuperados: 12
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