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| Coulombier, Noemie; Chim, Liet; Le Dean, Loic; Morezzi, Nicola; Brun, Pierre; Malo, Florent; Nakagawa, Kento; Ducrocq, Manuel; Rivaton, Adrien; Colin, Fabrice. |
| AMICAL is a partnership program of research and innovation between ADECAL TECHNOPOLE (New Caledonia innovation and technology park) and IFREMER to initiate the development of a microalgae industry in New Caledonia, based on selected local strains. This French oversea territory, located in south Pacific, features many competitive advantages for the development of a blue economy industry (climate, biodiversity hotspot, well developed aquaculture sector...). The project relies on two laboratories (Figure 1) owned and managed by ADECAL TECHNOPOLE with the scientific support of IFREMER: • the Laboratory for MicroAlgae study (LEMA), whose scientific direction is provided by the laboratory of Physiology and Biotechnology of Algae (PBA, IFREMER, Nantes),... |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00358/46897/46783.pdf |
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| Suquet, Marc; Malo, Florent; Quere, Claudie; Ledu, Christophe; Le Grand, Jacqueline; Benabdelmouna, Abdellah. |
| Triploidy induction in shellfish aims to obtain faster growth and sterility of reared individuals. Triploid Pacific oysters are most often not totally sterile, but have greatly reduced reproductive capacities compared to those recorded in diploid individuals. The description of gamete output in triploid aquatic animals is most often confined to the assessment of fecundity in females. The present work aims at further describing gamete quality of triploid Pacific oysters and comparing it to values observed in diploid individuals. Diploid and triploid oysters (produced by mating tetraploid males × diploid females) were reared in standard conditions and then transferred to the wild to allow the offspring to attain reproductive maturity. At the end of... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Reproduction; Triploidy; Gamete. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00277/38835/37581.pdf |
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| Suquet, Marc; Malo, Florent; Gourtay, Clemence; Ratiskol, Dominique; Riobe, Marion; Le Grand, Jacqueline; Queau, Isabelle; Mingant, Christian. |
| A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Mollusc; Sperm; Cryopreservation; Larval rearing. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00359/47033/46946.pdf |
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| Boulais, Myrina; Suquet, Marc; Arsenault-pernet, Marie Eve Julie; Malo, Florent; Queau, Isabelle; Pignet, Patricia; Ratiskol, Dominique; Le Grand, Jacqueline; Huber, Matthias; Cosson, Jacky. |
| Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster. The acidic pH of the gonad (5.82 ± 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared... |
| Tipo: Text |
Palavras-chave: Seminal fluid; Spermatozoa; Motility; PH; Salinity; Ions. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00428/53976/55137.pdf |
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| Suquet, Marc; Gourtay, Clemence; Donval, Anne; Le Goic, Nelly; Quere, Claudie; Malo, Florent; Le Grand, Jacqueline; Ratiskol, Dominique; Mingant, Christian; Fauvel, Christian. |
| Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol. Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Reproduction; Gamete; Sperm; Cryopreservation. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00317/42862/43912.pdf |
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