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Effectiveness of Cryptosporidium spp. oocysts detection and enumeration methods in water and milk samples Arq. Bras. Med. Vet. Zootec.
Machado,E.C.L.; Stamford,T.L.M.; Alves,L.C.; Melo,R.G.; Shinohara,N.K.S..
Cryptosporidium spp. oocyst recovery in water and milk samples was evaluated. Samples were inoculated with a suspension of 1.2×10(7) Cryptosporidium spp. oocysts and submitted to centrifugal flotation, using different solutions (sucrose, NaCl, MgSO4, ZnSO4, AlSO4, NH4SO4 40% and NH4SO4 80%). Centrifugation of the samples was carried out in two stages for concentration using two methods that differed in the order in which the saturated solutions were used, namely only in the first stage of method I and only in the second stage of method II. Oocyst identification was performed using the Kinyoun and Koster histochemical staining techniques. Samples analyzed by method I showed different degree of oocyst recovery, namely 10.9% with NaCl and 42.5% with MgSO4 in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oocyst; Cryptosporidium spp.; Concentration methods; Water; Milk.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300023
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